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821893 Supelco

ToyoScreen® Phenyl-600M Process Development Column

phase phenyl, pkg of 6 × 5 mL, 65 μm particle size



Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories, ToyoScreen Hydrophobic Interaction Process Development Columns,
material   aluminum seal
  polypropylene and polyethylene frit
packaging   pkg of 6 × 5 mL
mfr. no.   Tosoh, 21893
parameter   ≤50% organic solvent
  1-5 mL/min flow rate
  20 mL/min max. flow rate
  3 bar max. pressure
L × I.D.   3 cm × 14.6 mm
matrix   hydroxylated methacrylic polymer
matrix active group   phenyl phase
particle size   65 μm
operating pH   1 - 13
capacity   55 g/L±10 adsorption capacity (lysozyme)
separation technique   hydrophobic interaction (HIC)


General description

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

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Clean in place with 0.5 M NaOH or 0.1 M HCl.

Legal Information

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation

Safety & Documentation

Safety Information

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Certificate of Analysis

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Protocols & Articles

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