• USA Home
  • 821982 - TSKgel® Amide-80 HPLC Column


TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size

Synonym: TSKgel® Amide-80 HR

  •  eCl@ss 32110501



Related Categories 5 and 10 μm TSKgel Amide-80 and Amide-80 HR Columns, Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, TSKgel HILIC HPLC Columns,
material   stainless steel hardware
description   Amide-80 HR
mfr. no.   Tosoh, 21982
parameter   0-100% organic solvent
  10-80 °C temperature
  150 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
matrix   silica particle platform
matrix active group   carbamoyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH   2.0 - 7.5
suitability   suitable for L68 per USP
separation technique   hydrophilic interaction (HILIC)


General description

TSKgel Amide-80 columns are now also available packed with 2 μm spherical silica particles, offering higher resolution and faster analysis with equivalent retention and selectivity as TSKgel Amide-80, 3 μm columns. This allows for smooth method transfer from a 3 μm to a 2 μm column. In addition, TSKgel Amide-80, 2 μm columns more highly retain hydrophilic compounds than competitive amide columns on the market.
Packed with 2, 3, 5, or 10 μm spherical silica particles that are covalently bonded with carbamoyl groups, TSKgel Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.
TSKgel Amide-80 HR, 5 μm columns offer higher specifications for plate count compared to traditional TSKgel Amide-80, 5 μm, 4.6 mm ID x 25 cm columns (N = > 18,000 vs. 8,000). This equates to more efficient chromatography.

Physical form

Shipped in 75% acetonitrile - 25% water.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?