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TSKgel® Butyl-NPR HPLC Column

phase C4 (butyl), L × I.D. 10 cm × 4.6 mm, 2.5 μm particle size



Related Categories Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Hydrophobic Interaction (HIC) Columns, TSKgel Butyl-NPR Column (non-porous),
material   stainless steel
mfr. no.   Tosoh, 42168
parameter   ≤4 M salt concentration
  ≤50% organic solvent
  1.5 mL/min flow rate
  10-60 °C temperature
  200 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   10 cm × 4.6 mm
matrix   hydroxylated methacrylic polymer
matrix active group   C4 (butyl) phase
particle size   2.5 μm
operating pH   2 - 12
separation technique   hydrophobic interaction (HIC)


General description

TSKgel® Butyl-NPR columns are not as hydrophobic, i.e., less retentive, than Phenyl-5PW and Ether-5PW HIC columns. They are the best choice for high speed separations and provide excellent recovery even for more hydrophobic proteins. The non-porous resin requires lower sample loading and leads to faster analysis time because the binding kinetics occur only on the bead′s surface. The ultra efficient 2.5 micron bead allows for fast, high efficiency separations using a 3.5cm x 4.6mm ID column format. Thus, TSKgel Butyl-NPR is ideal for time critical QC analysis or sample limited applications. Featuring polymethacrylate base material, the TSKgel Butyl-NPR column is stable in either acid or caustic cleaning regimes up to 1N.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Protocols & Articles


HPLC Analysis of of Fab and Fc Fragments on TSKgel® Butyl-NPR

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of of Fab and Fc Fragments on TSKgel® Butyl-NPR
Keywords: Chromatography, High performance liquid chromatography

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