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1085003 USP

Caffeine

United States Pharmacopeia (USP) Reference Standard

Synonym: 1,3,7-Trimethylxanthine

  • CAS Number 58-08-2

  • Empirical Formula (Hill Notation) C8H10N4O2

  • Molecular Weight 194.19

  •  Beilstein Registry Number 17705

  •  MDL number MFCD00005758

  •  PubChem Substance ID 329749194

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, Pharmacopeia & Metrological Institutes Standards, USP Standards, USP Standards C - D More...
InChI Key   RYYVLZVUVIJVGH-UHFFFAOYSA-N
form   neat
mp   234-236.5 °C (lit.)
Featured Industry   Pharmaceutical (small molecule)
format   neat

Description

Analysis Note

These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind.  ​

General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia.For further information and support please go to the website of the issuing Pharmacopoeia.

Other Notes

Sales restrictions may apply.

USP issued SDS can be found here.

Application

Caffeine is used as an internal standard in the quantitative determination of the trihydrate and anhydrous forms of ampicillin and its sodium salt in drug samples using reverse-phase high performance liquid chromatography (RP-HPLC). It is also used as a reference standard in determining the concentration of caffeine present, in samples of human serum using high performance liquid chromatography (HPLC).

Biochem/physiol Actions

A central nervous system stimulant believed to act through adenosine receptors and monoamine neurotransmitters. It is an adenosine receptor antagonist and adenosine 3′,5′-cyclic monophosphate (cAMP) phosphodiesterase inhibitor. Thus, levels of cAMP increase in cells following treatment with caffeine. It has been reported to affect cellular calcium levels, releasing calcium from intracellular stores. It overrides the cell cycle effects of various chemicals such as protease inhibitors, preventing apoptosis; and it has been shown to inhibit cellular DNA repair mechanisms.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Same Products - New Packaging
Protocols & Articles

Articles

HPLC Analysis of Caffeine, Choline, Taurine, Inositol, and Carnitine on SeQuant ZIC-HILIC

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Caffeine, Choline, Taurine, Inositol, and Carnitine on SeQuant ZIC-HILIC
Keywords: High performance liquid chromatography

HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)
Keywords: Analgesics, Chromatography, Herbicides, High performance liquid chromatography, Ion Exchange, Pesticides, Pharmaceutical

HPLC Analysis of Xanthines on Purospher®STAR RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Xanthines on Purospher®STAR RP-18 endcapped
Keywords: Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical, Separation

LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

TLC Analysis Analgesics and Preservatives on HPTLC Silica Gel 60 RP-18 WF254s (analyte set 4)

From our library of Articles, Sigma-Aldrich presents TLC Analysis Analgesics and Preservatives on HPTLC Silica Gel 60 RP-18 WF254s (analyte set 4)
Keywords: Chromatography, Pharmaceutical, Thin layer chromatography

TLC Analysis of Alkaloids by Ion-Pair Chromatography on HPTLC Silica Gel 60 RP-8 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Alkaloids by Ion-Pair Chromatography on HPTLC Silica Gel 60 RP-8 F254s
Keywords: Chromatography, Pharmaceutical, Thin layer chromatography

TLC Analysis of Ingredients in Anti-Migraine Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 2)

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Ingredients in Anti-Migraine Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 2)
Keywords: Thin layer chromatography

TLC Analysis of Ingredients in Antirheumatic Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 1)

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Ingredients in Antirheumatic Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 1)
Keywords: Thin layer chromatography

TLC Analysis of Ingredients in Antirheumatic Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 2)

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Ingredients in Antirheumatic Agents on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone (analyte set 2)
Keywords: Thin layer chromatography

TLC Analysis of N-Methyl Xanthine Derivatives on HPTLC NH2 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis of N-Methyl Xanthine Derivatives on HPTLC NH2 F254s
Keywords: Thin layer chromatography, Vitamins

TLC Analysis of Nitrophenols on HPTLC Pre-Coated Plate CN F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Nitrophenols on HPTLC Pre-Coated Plate CN F254s
Keywords: Thin layer chromatography

TLC Analysis of Pharmaceutical Products on HPTLC Silica Gel 60 F 254

From our library of Articles, Sigma-Aldrich presents TLC Analysis of Pharmaceutical Products on HPTLC Silica Gel 60 F 254
Keywords: Pharmaceutical, Thin layer chromatography

TLC analysis of Catecholamines by ion-pair chromatography on TLC pre-coated plate RP-8 F 254s

From our library of Articles, Sigma-Aldrich presents TLC analysis of Catecholamines by ion-pair chromatography on TLC pre-coated plate RP-8 F 254s
Keywords: Thin layer chromatography

Peer-Reviewed Papers
15

References

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PHR1009 Caffeine, Pharmaceutical Secondary Standard; Certified Reference Material

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