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638 matches found for BAR

HPLC Analysis of HSA Fusion Protein on Proteomix® SAX-NP5

column Proteomix SAX-NP5 25 cm x 4.6mm I.D., 5 µm particles (Z777229) column temp. 30 °C mobile phase Piperazine, Triethanolamine, bis-tris propane and N-methylpiperazine, 20 mM each,(A) pH 9.7; (B) pH 3.0 ( adjust by HCl ) flow rate 0.5 mL/min pressure 2016 psi (139 bar) sample HSA fusion peptide 5...

HPLC Analysis of DNA on Proteomix® WAX-NP5

column Proteomix WAX-NP5, 15 cm x 4.6 mm I.D., 5 µm particles (Z777262) mobile phase (A) 20 mmol Tris, pH 6.0; (B) A + 1.0 M NaCl gradient 20 to 100% B in 40 min flow rate 0.6 mL/min pressure 1015 psi (70 bar) temp. ambient sample DNA injection 5 µL detector UV, 260 nm Categories Analytical Chromato...

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

column Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U) column temp. 35 °C mobile phase [A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B) flow rate 1.0 mL/min pressure 1247 psi (86 bar) injection 5 µL detector ESI(+); SIR Analysis ...

LC/MS (TOF) Analysis of 2C Amine Drugs of Abuse on Ascentis® Express HILIC

column Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 µm particles (53934-U) column temp. 35 °C mobile phase 2 mM ammonium formate in 90:10 (v:v) acetonitrile:water flow rate 0.4 mL/min pressure 943 psi (65 bar) sample 300 ng/mL in acetonitrile injection 2 µL detector TOF/MS Analysis Note The 2C am...

LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express OH5

column Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U) column temp. 35 °C mobile phase 5 mM ammonium formate in 95:5, acetonitrile:water, adjusted to pH 4.0 with formic acid flow rate 0.4 mL/min pressure 1200 psi (83 bar) sample ethyl sulfate (2 µg/mL), ethyl-beta-D-glucuronide...

HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express 2.7 μm RP-Amide

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U) column temp. 35 °C mobile phase [A] 25 mM ammonium phosphate pH 7.0 with 30% Phosporic Acid; [B] methanol; (30:70, A:B) flow rate 0.3 mL/min pressure 3162 psi (218 bar) sample Amitriptyline, Clomipramine, Desipramine, ...

LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express RP-Amide

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U) column temp. 35 °C mobile phase 0.1% (v/v) formic acid in water flow rate 0.4 mL/min pressure 3066 psi (211 bar) sample 2 µg/mL in 99:1, water:methanol injection 2 µL detector MS, ESI(-), combined XIC, 125.0 m/z, 221.1...

HPLC Analysis of Bimatoprost on Chromolith® Performance 100-3mm

column Chromolith® Performance 100 x 3 mm (1.52001.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water + 0,05% TFA v/v; [C] metanol; (30:60:10, A:B:C) flow rate 850 μL/min pressure 10827 psi (747 bar) sample Bimatoprost (1mg/mL), Bimatoprost free acid (1mg/mL) injection 2 μL detector...

HPLC Analysis of Acidic Drugs on Chromolith® Performance 100- 4 mm (analyte set 2)

column Chromolith® Performance 100 x 4 mm (1.52028.0001) column temp. ambient mobile phase [A] acetonitrile; [B] ammoniumacetate 20mM pH=6,8; (28:70, A:B) flow rate 2 mL/min (u=2mm/s) pressure 5629 psi (39 bar) sample Indoprofen, Naprofen, Ethacrynic acid, Ibuprofen, Indomethacine injection 5.0 μL d...

LC/MS Analysis of Sodium Cyclamate on SeQuant® ZIC-HILIC

column SeQuant® ZIC-cHILIC, PEEK, 150 x 2.1 mm, 3 μm, 100Ã… (1.50658.0001) column temp. ambient mobile phase [A] acetonitrile; [B] 100 mM ammonium acetate adjusted to pH 4.5 by glacial acetic acid; (75:25, A:B) flow rate 0.2 mL/min pressure 566 psi 39 Bar sample 1.0 mg/mL sodium cyclamate in diluent...

UHPLC Analysis of Haloperidol and Metabolite on Titan™ C18

column Titan C18 5 cm x 2.1 mm, 1.9 µm particles (577122-U) column temp. 35 °C mobile phase [A] 10 mM potassium phosphate, monobasic pH 3.0; [B] acetonitrile; (70:30, A:B) flow rate 0.6 mL/min pressure 4310 psi (297 bar) sample 1-10 µg/mL in 70:30, water:methanol injection 1 µL detector UV, 200 nm C...

LC/MS Analysis of Hydroxyamphetamine Hydrobromide on SeQuant® ZIC-HILIC

column SeQuant® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Ã… (1.50661.0001) column temp. 25 °C mobile phase [A] acetonitrile; [B] 1.361 g of monopotassium phosphate in 1000 ml water (10mM); (70:30, A:B) flow rate 1.0 mL/min pressure 1174.5 psi (81 bar) sample Hydroxyamphetamine Hydrobromide RS 100 p...

UHPLC Analysis of Meropenem and Biapenem Antiobiotics on Ascentis® Express HILIC

column Ascentis Express HILIC, 10 cm x 3.0 mm, 2 µm particles (51428-U) column temp. 35 °C mobile phase [A] 100 mM ammonium formate: [B] acetonitrile: (25:75), pH to 5 with formic acid flow rate 0.5 mL/min pressure 1800 psi (193 bar) sample 50 ug/mL each in 90% acetonitrile injection 5 µL detector U...

HPLC Analysis of Bimatoprost on Chromolith® Performance 100-2mm

column Chromolith® Performance, 100 x 2 mm (1.52006.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water + 0,05% TFA v/v; [C] methanol; (30:60:10, A:B:C) flow rate 380 μL/min pressure 691 psi (48 bar) sample Bimatoprost (1mg/mL), Bimatoprost free acid (1mg/mL) injection 1 μL detector ...

LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2

column Astec CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 µm particles (15023AST) column temp. 20 °C mobile phase [A] methanol; [B] water; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.1:0.02, A:B:C:D flow rate 1 mL/min pressure 1220 psi (84 bar) sample 50 ng/mL each enantiomer in methanol injection 2 ...

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U) column temp. 35 °C mobile phase [A] 10mM ammonium formate in water; [B] 10mM ammonium formate in acetonitrile; (32:68, A:B) flow rate 0.4 mL/min pressure 1711 psi (118 bar) sample 10 µg/mL in 25:75, water:methanol injection ...

LC/MS Analysis of Sodium, Potassium and Chloride on SeQuant® ZIC-HILIC

column SeQuant ZIC-cHILIC, PEEK, 150 x 4.6 mm, 5 μm, 100 Ã… (1.50461.0001) column temp. 30 °C mobile phase [A] acetonitrile; [B] ammonium acetate 62.5mM pH 5; (80:20, A:B) flow rate 1.0 mL/min pressure 1088 psi 75 Bar sample 100 μg/mL KCl and 100 μg/mL NaCl injection 20 μL detector Sedere Sedex 85 E...

HPLC Analysis of α-Pyrrolidinobutiophenone (PBP) Enantiomers on Astec® CHIROBIOTIC® V2

column Astec CHIROBIOTIC V2, 15 cm x 4.6 mm I.D., 5 µm particles (15023AST) column temp. 20 °C mobile phase [A] methanol; [B] water; [C] acetic acid; [D] ammonium hydroxide; (95:5:0.1:0.02, A:B:C:D) flow rate 1 mL/min pressure 1100 psi (70 bar) sample 100 µg/mL in methanol injection 5 µL detector UV...

HPLC Analysis of Ergosterol in Vacuum Cleaner Dust on Chromolith® Performance 100-2mm

sample preparation See "Analysis Note." column Chromolith® Performance, 100 x 2 mm (1.52006.0001) column temp. 23 °C mobile phase [A] methanol; [B] Water; (98:2, A:B) flow rate 190 μL/min pressure 595 psi 41 Bar sample Standard: Ergosterol; Matrix: Dust of a vacuum cleaner injection 1 μL detector 28...

HPLC Analysis of B-Vitamins on Chromolith NH2 100 - 4.6mm

column Chromolith® NH2 100 x 4.6 mm (1.52028.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water; (20:80, A:B) gradient 0-3min 1mL/min, 3,1-10min 2mL/min pressure 290 psi (7-20 bar) sample Riboflavin (8.8mg/10ml), Pyridoxin ( 5.5mg/10ml), Cyanocobalamin (5.2mg/10ml) injection 2 μL de...

HPLC Analysis of EPA 8330 Mix A and EPA 8330 Mix B on Ascentis® Express C18

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 µm particles (50537-U) column temp. 42 °C mobile phase mobile phase: [A] water [B] methanol (72:28, A:B) flow rate 2.00 mL/min pressure 3277 psi (226 bar) sample 0.033 mg/mL in water/acetonitrile; 33:67 injection 10 µL detector UV, 254 nm Categorie...

UHPLC Analyis of Protein Standards on Sepax Unix™ SEC-300: Column Performance as a Function of Flow Rate

column Sepax Unix SEC-300, 15 cm x 4.6 mm I.D., 1.8 µm particles column temp. 25 °C mobile phase 0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid flow rate 0.3 mL/min flow rate 0.1 mL/min flow rate 0.5 mL/min pressure 1550 psi (107 bar) pressure 2400 psi (165 bar) pressure 500 psi (34 ba...

LC/MS Analysis of Illicit Bath Salts on Ascentis® Express HILIC, Set 4

column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U) column temp. 35 °C mobile phase 5 mM ammonium formate in [A] acetonitrile; [B] water; (98:2, A:B) flow rate 0.6 mL/min pressure 267 psi (18.4 bar) sample 500 ng/mL in acetonitrile injection 2 µL detector MS, ESI(+) TOF, S...

HPLC Analysis of Voriconazole Without Stress on Chromolith Performance 100-3mm

column Chromolith® Performance 100 x 3 mm (1.52001.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water; (50:50, A:B) flow rate 425 μL/min pressure 4032 psi (28 bar) sample Voriconazole 50 μg/mL injection 5 μL detector 256 nm cell: 1.4 μL Featured Industry Pharmaceutical (small molecu...

HPLC Analysis of Bimatoprost on Chromolith® Performance 25-2mm

column Chromolith® Performance, 25 x 2 mm (1.52014.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water + 0,05% TFA v/v; [C] methanol; (25:70:5, A:B:C) flow rate 190 μL/min pressure 3898 psi (269 bar) sample Bimatoprost (1mg/mL), Bimatoprost free acid (1mg/mL) injection 1 μL detector ...

HPLC Analysis of Magnesium Ascorbyl Phosphate (MAP) on Chromolith® NH2 50 - 4.6mm

column Chromolith® NH2, 50 x 4.6 mm (1.52027.0001) column temp. 23 °C mobile phase [A] acetonitrile; [B] Na-buffer 10mM pH=4.0; (20:80, A:B) flow rate 2 mL/min pressure 421 psi (29 bar) sample Magnesiumascorbylphosphate, 100μg/ml injection 1 μL detector 255 nm cell: 11 μL Featured Industry Vitamins,...

LC/MS Analysis of Isobaric Bath Salts Ethylone and Butylone on Ascentis® Express HILIC

column Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 µm particles (53934-U) column temp. 35 °C mobile phase 5 mM ammonium formate (98:2 acetonitrile:water) flow rate 0.6 mL/min pressure 1262 psi (87 bar) sample 20 ng/mL injection 1 µL detector ESI(+), 222 m/z Categories Analytical Chromatography, ...

HPLC Analysis of Oligonucleotides on Proteomix® SAX

column Proteomix SAX-NP5, 25 cm x 4.6 mm I.D., 5 µm particles (Z777229) column temp. 25 °C mobile phase A: 20 mM Tris, pH 8.0 B: A + 0.5 M NaCl gradient 0 - 100% B in 30 minutes flow rate 0.5 mL/min pressure 1073 psi (74 bar) sample (10 mM each oligonucleotide in water) injection 5 µL detector UV 26...

SFC Analysis of Diastereomers on Astec® Cellulose DMP, Methanol Additive

column Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 µm particles (51098AST) column temp. 35 °C mobile phase [A] CO2; [B] methanol; (80:20, A:B) flow rate 3 mL/min pressure 1450 psi (100 bar) injection 5 µL detector UV, 220 nm Analysis Note This application shows the ability of Astec Cellulose DMP to ...

UHPLC Analysis of Diazepam and Metabolites on Titan™ C18, Competitive Comparison

column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U) column temp. 35 °C mobile phase [A] 0.1% formic acid; [B] acetonitrile; (60:40, A:B) pressure 6450 psi (445 bar) detector UV, 254 nm Analysis Note This application shows a comparison between Titan™ C18 and a competitive column for di...