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    Search term:"Endotoxin-Free Plasmid"

    106 matches found for Endotoxin-Free Plasmid

    GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (208 KB )

    The basic structure of an endotoxin is similar among Gram negative bacteria but the chemical composition can vary widely. The structure consists of three distinct regions: lipid A, core oligosaccharide and O-antigen (Figure 1). Lipid A is the hydrophobic, membrane-anchoring region of the endotoxin a...

    PLED35 - Datasheet (59 KB)

    Harvest & lyse bacteria ❑ Pellet cells from up to 40 ml overnight culture at 3,000-5,000 x g, 10 min. Discard supernatant. ❑ Add 1.2 ml of Resuspension Solution. Resuspend the pellet until homogeneous. ❑ Add 1.2 ml of Lysis Solution. Invert gently to mix. Incubate at room temperature for ≤ 5 min. *P...

    NA0400 - Vacuum Protocol - Bulletin (896KB)

    GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit User Guide Catalog Nos. NA0400 NA0400S NA0410 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0400S GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 4 preps NA0400 GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 10 preps NA041...

    NA0400S - Vacuum Protocol - Bulletin (896KB)

    GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit User Guide Catalog Nos. NA0400 NA0400S NA0410 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0400S GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 4 preps NA0400 GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 10 preps NA041...

    PLED25 - Bulletin (34 KB)

    GenElute™ Endotoxin-free Plasmid Midiprep Kit Product No. PLED 25 Technical Bulletin MB-845 May 2000 TECHNICAL BULLETIN Product Description Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sen...

    NA0410 - Vacuum Protocol - Bulletin (577 KB)

    GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit User Guide Catalog Nos. NA0400 NA0400S NA0410 sigma.com Ordering Information Cat. No. Product Description Pkg Size NA0400S GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 4 preps NA0400 GenElute HP Endotoxin-Free Plasmid Maxiprep Kit 10 preps NA0410 G...

    PLED35 - Bulletin (354 KB)

    GenElute™ Endotoxin-free Plasmid Midiprep Kit User Guide Catalog Nos. PLED35 sigma.com Ordering Information Cat. No. Product Description Pkg Size PLED35 GenElute™ Endotoxin-free Plasmid Midiprep Kit 35 preps Related Products Related Products Cat. No. Related Products Cat. No. Water, Molecular Biolog...

    PLEX10 - Bulletin (31 KB)

    GenElute™ Endotoxin-free Plasmid Maxiprep Kit Product No. PLEX 10 Technical Bulletin MB-875 May 2000 TECHNICAL BULLETIN Product Description Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sen...

    NA0600 - Bulletin (535KB)

    GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit User Guide Catalog Nos. NA0600 sigma.com Ordering Information Cat. No. Product Description Pkg Size NA0600 GenElute HP Endotoxin-Free Plasmid Megaprep Kit 5 preps Related Products Cat. No. Product Description Pkg Size NA0150 GenElute HP Plasmid Minipr...

    PLEX15 - Bulletin (1MB)

    GenElute™ Endotoxin-free Plasmid Maxiprep Kit USER GUIDE Catalog Number PLEX15 Ordering Information Catalog No. Product Description Pkg Size PLEX15 GenElute™ Endotoxin-free Plasmid Maxiprep Kit 15 preps Related Products Related Products Catalog No. Related Products Catalog No. Water, Molecular Biolo...

    Pled 35 (194 KB)

    GENELUTE ENDOTOXIN-FREE PLASMID MIDIPREP KIT Order/Customer Service Phone 1-800-325-3010 Fax 1-800-325-5052 Technical Service 1-800-325-5832 World Headquarters 3050 Spruce St. St. Louis MO 63103 (314)771-5750 www.sigma-aldrich.com PLED 35 1 Harvest & lyse bacteria ❑ Pellet cells from up to 40 ml ove...

    Plex 15 (166 KB)

    GENELUTE ENDOTOXIN-FREE PLASMID MAXIPREP KIT 1 Order/Customer Service Phone 1-800-325-3010 Fax 1-800-325-5052 Technical Service 1-800-325-5832 World Headquarters 3050 Spruce St. St. Louis MO 63103 (314)771-5750 www.sigma-aldrich.com PLEX 15 Harvest & lyse bacteria ❑ Pellet cells from up to 130 ml ov...

    Technical Article Jul00 (247 KB )

    The GenElute™ Endotoxin-free DNA Purification Kit: Pure DNA for Endotoxin-Sensitive Applications by Fuqiang Chen, Scott Weber and Jo Anne Kerschner Sigma-Aldrich Corporation, St. Louis, MO, USA Introduction Endotoxins are lipopolysaccharides (LPS), found among outer cell-membrane components of Gram ...

    NA0410 - Spin Protocol - Bulletin (274 KB)

    Spin Format Protocol Modification GenElute™ High Performance (HP) Endotoxin-Free Plasmid Maxiprep Kit Product Codes NA0400S, NA0400, NA0410 TECHNICAL BULLETIN Product Description Endotoxins are a common contaminant in plasmid preparations that can reduce transfection efficiencies in sensitive eukary...

    NA0400S - Spin Protocol - Bulletin (274 KB)

    Spin Format Protocol Modification GenElute™ High Performance (HP) Endotoxin-Free Plasmid Maxiprep Kit Product Codes NA0400S, NA0400, NA0410 TECHNICAL BULLETIN Product Description Endotoxins are a common contaminant in plasmid preparations that can reduce transfection efficiencies in sensitive eukary...

    NA0400 - Spin Protocol - Bulletin (274 KB)

    Spin Format Protocol Modification GenElute™ High Performance (HP) Endotoxin-Free Plasmid Maxiprep Kit Product Codes NA0400S, NA0400, NA0410 TECHNICAL BULLETIN Product Description Endotoxins are a common contaminant in plasmid preparations that can reduce transfection efficiencies in sensitive eukary...

    HP Plasmid DNA Purification (4.3 MB )

    Plasmid DNA Purification 4 sigma.com/nap ORDER: 800-325-3010 | TECHNICAL SERVICE: 800-325-5832 HP Plasmid DNA Purification Five-minute Miniprep Endotoxin-free High Performance High Performance Sigma’s GenElute ™ DNA Plasmid Purification Kits A Kit for Every Application — DNA Never Had It So Good! Pl...

    NA0500 - Bulletin (700KB)

    GenElute™ HP Plasmid Megaprep Kit User Guide Catalog No. NA0500 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0500 GenElute HP Plasmid Megaprep Kit 5 preps Related Products Catalog No. Product Description Pkg Size NA0150 GenElute HP Plasmid Miniprep Kit 70 preps NA0200 Ge...

    FAQs LSQ Vol6 Issue1 (111 KB)

    7 Order: 1.800.325.3010 Technical Service: 1.800.325.5832 What size does the kit come in? The kit is available in 4-, 10- and 25-prep sizes. Are additional materials needed to use this kit? Yes. One of the wash solutions included in the kit requires the addition of ethanol. How does the HP Endotoxin...

    NA0800 - Bulletin (543 KB)

    GenElute™ HP Select Plasmid Gigaprep Kit User Guide Catalog Nos. NA0800 sigma.com Ordering Information Catalog Number Product Description Pkg Size NA0800 GenElute HP Select Plasmid Gigaprep Kit 5 preps Related Products Catalog Number Product Description Pkg Size NA0150 GenElute HP Plasmid Miniprep K...

    NA0300 - Bulletin (517KB)

    GenElute™ HP Plasmid Maxiprep Kit User Guide Catalog Nos. NA0300 NA0300S NA0310 sigma.com Ordering Information Cat. No. Product Description Pkg Size NA0150 GenElute HP Plasmid Miniprep Kit 70 preps NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps NA03...

    NA0200S - Bulletin (682 KB)

    GenElute™ HP Plasmid Midiprep Kit User Guide Catalog Nos. NA0200S NA0200 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps Related Products Catalog No. Product Description Pkg Size...

    NA0310 - Bulletin (517KB)

    GenElute™ HP Plasmid Maxiprep Kit User Guide Catalog Nos. NA0300 NA0300S NA0310 sigma.com Ordering Information Cat. No. Product Description Pkg Size NA0150 GenElute HP Plasmid Miniprep Kit 70 preps NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps NA03...

    NA0200S - Bulletin (682 KB)

    GenElute™ HP Plasmid Midiprep Kit User Guide Catalog Nos. NA0200S NA0200 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps Related Products Catalog No. Product Description Pkg Size...

    NA0200 - Bulletin (682 KB)

    GenElute™ HP Plasmid Midiprep Kit User Guide Catalog Nos. NA0200S NA0200 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps Related Products Catalog No. Product Description Pkg Size...

    NA0300S - Bulletin (517KB)

    GenElute™ HP Plasmid Maxiprep Kit User Guide Catalog Nos. NA0300 NA0300S NA0310 sigma.com Ordering Information Cat. No. Product Description Pkg Size NA0150 GenElute HP Plasmid Miniprep Kit 70 preps NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps NA03...

    NA0200 - Bulletin (682 KB)

    GenElute™ HP Plasmid Midiprep Kit User Guide Catalog Nos. NA0200S NA0200 sigma.com Ordering Information Catalog No. Product Description Pkg Size NA0200S GenElute HP Plasmid Midiprep Kit 4 preps NA0200 GenElute HP Plasmid Midiprep Kit 25 preps Related Products Catalog No. Product Description Pkg Size...

    renap (1 MB )

    Applications Bioreagents for Nucleic Acid Purification Cloning DNA/RNA Electrophoresis Northern/Southern Blotting Cell Culture RNAi PCR Protein Electrophoresis Transfection Western Blotting Nucleic Acid Purification Protein Expression and Purification Applications Bioreagents for Nucleic Acid Purifi...

    PLN10 - Bulletin (307 KB)

    GenElute™ Plasmid Miniprep Kit User Guide Catalog Nos. PLN10 PLN70 PLN350 sigma.com Ordering Information Catalog No. Product Description Pkg Size PLN10 GenElute Plasmid Miniprep Kit 10 preps PLN70 GenElute Plasmid Miniprep Kit 70 preps PLN350 GenElute Plasmid Miniprep Kit 350 preps Related Products ...

    PFM250 - Bulletin (598KB)

    GenElute™ HP Five–Minute Plasmid Miniprep Kit User Guide Catalog Nos. PFM10 PFM50 PFM250 sigma.com Ordering Information Catalog No. Product Description Pkg Size PFM10 GenElute Five-Minute Plasmid Miniprep Kit 10 preps PFM50 GenElute Five-Minute Plasmid Miniprep Kit 50 preps PFM250 GenElute Five-Minu...