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Antibody microarrays(1)
Atomic absorption spectroscopy(1)
Capture ELISA(2)
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Detergents(1)
Direct immunofluorescence(1)
Dot blot(1)
Enzyme-linked immunosorbent assay(2)
Flow cytometry(2)
Fractionation(1)
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Immunoassay(1)
Immunocytochemistry(2)
Immunoelectrophoresis(2)
Immunofluorescence(2)
Immunohistochemistry(2)
Immunoprecipitation(2)
Indirect ELISA(2)
Individual protein Immunoprecipitation(2)
Infrared spectroscopy(1)
Ion-exchange chromatography(1)
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Microarray Analysis(2)
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Protein extraction(1)
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Disease
Cancer(2)
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Gene expression(1)
Lipid Metabolism(1)
Metabolism(1)
Morphogenesis(2)
Oxidations(1)
Phosphorylations(3)
Precipitation(1)
Rearrangements(1)
Transcription(1)
transformation(2)
Research Area
Proteomics(1)

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Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gen...

細胞小器官マーカー用抗体

新しいタンパク質が発見された際、その機能解析だけでなく細胞内局在も重要な情報の1つです。さらに、細胞内局在を知ることは、そのタンパク質の機能を知る上で大変な助けとなります。タンパク質の局在を調べる最も一般的な手法の1つは、タンパク質特異的な抗体と細胞小器官特異的な抗体を用いて2重染色を行うことです。 シグマアルドリッチでは、細胞小器官マーカーとして有用な細胞小器官特異的もしくは細胞小器官に局在するタンパク質特異的な多くの抗体ラインナップをご提供いたします。これらの細胞小器官マーカーを用いることで共局在の有無を調べることも可能ですし、細胞小器官を単離する際には濃縮率を測定することも可能です。 シ...

Organelle Isolation & Analysis Kits

Enriching for organelles simplifies proteomic analysis and when partnered with protein pathway function allows key insight into development of healthy cell function as well as the disruption that leads to disease. The kits and reagents shown below enable researchers to enrich for functional mitochon...

STE20

The Ste20 (Sterile 20) protein kinase family consists of enzymes with sequence homology to the yeast MAP4K Ste20p in the pheromone response pathway. Based on phylogenetic relationships, they can be divided into two subfamilies: PAK (p21-activated kinase) and GCK (germinal center kinase). The PAKs in...

Src

Since the isolation of v-Src as the transforming component of Rous sarcoma virus, and the subsequent identification of its cellular homolog c-Src, there has been intense interest in its activity and regulation. Src is the founding member of a group of non-receptor protein tyrosine kinases termed the...