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Search term:"Glycine"

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237 matches found for Glycine

Coupling through Hydroxy, Amino, or Thiol Groups via a 12-Carbon Spacer Arm

Epoxy-activated Sepharose 6B is used for coupling ligands that contain hydroxyl, amino, or thiol groups. Because of the long hydrophilic spacer arm, it is particularly useful for coupling small ligands such as choline, ethanolamine, and sugars. The preactivated matrix is formed by reacting Sepharose...

Immunoprecipitation of FLAG fusion proteins using monoclonal antibody affinity gels

Related Links • FLAG® Tag • FLAG® Tag Bacterial and Mammalian Expression Vectors • FLAG® System • FLAG® Antibodies • FLAG® Affinity Purification • 3X FLAG • What is the FLAG peptide tag, and how is it used to isolate and purify proteins? • Immunoprecipitation technical overview • Protocol for immuno...

Preactivated Magnetic Beads

Preactivated magnetic beads are designed for covalent coupling of antibodies, aptamers, and proteins. After coupling is performed, proteins of interest can be affinity captured and enriched using immunoprecipitation (see example in Fig 5.7). The preactivated magnetic beads include NHS Mag Sepharose, ...

Purification or Removal of Biotin and Biotinylated Substances

Biotin and biotinylated substances bind to streptavidin, a molecule isolated from Streptomyces avidinii. The binding of streptavidin to biotin is one of the strongest known noncovalent biological interactions. Hence, denaturing conditions are generally required for the efficient elution of biotinylat...

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents across multiple global locations by using a single lot of material. Our distribution capabilities and r...

Astec CHIROBIOTIC™ Chiral HPLC Column Literature References

Astec Products | Technical Resources 1. Macrocyclic Antibiotics as a New Class of Chiral Selectors for Liquid Chromatography, D. W. Armstrong, Y. Tang, S. Chen. Y. Zhou, C. Bagwell, J-R. Chen, Analytical Chemistry, Vol. 66, No. 9, 1473-1484 (1994). 2. A Covalently Bonded Teicoplanin Chiral Stationar...

Assay Procedure for Glycerokinase

The appearance of NADH is measured at 340nm by spectrophotometry. One unit causes the formation of one micromole of NADH per minute under the conditions described below. Reagents A. Glycine-hydrazine buffer, pH 9.8 :0.2M[Weigh 1.5g of glycine and 20.8g of hydrazine hydrate (MW= 50.06), dissolve in 7...

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

To standardize a procedure for Enzymatic Assay of b-Glucuronidase1. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. One Sigma or modified "Fishman" unit will liberate 1.0 microgram of Phenolphthalein from Phenolphthalein Glucuronide per ho...

Biochemicals Amino Acids

General Literature Introduction In Living Systems. Amino acids play many important roles in living systems. They are used as building blocks for peptides which in turn serve to provide structure, transport or catalysis in the organisms. Furthermore amino acids act as metabolic intermediates and subs...

glycine dehydrogenase (decarboxylating)
Synonyms GCE, GCSP, HYGN1
Species Human (2731) , Species Mouse (104174) , Species Rat (309312) , Species Zebrafish (321621) , Species domestic cat (101100163) , Species cow (507688) , Species Horse (100060355) , Species dog (481534) , Species chicken (374222) , Species sheep (101117476) , Species Domestic Rabbit (100343694) , Species domestic guinea pig (100714862) , Species naked mole-rat (101725465)
Summary: Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010]

Enzyme Class Index: Hydrolases on esters

We offer over 1,600 enzymes as well as associated inhibitors and substrates. TO SEARCH FOR AN ENZYME BY EC # OR REACTION CATALYZED... 1. Click on a category in the menu below 2. Scroll down the list to the enzyme of interest 3. Click on the Product # to access product listing and related information...

Purification or Removal of Albumin and IgG

Albumin and IgG are the most abundant proteins in plasma which tend to obscure the signals of less abundant proteins, preventing accurate detection. The high abundance of albumin and IgG also interferes with the detection of other proteins by preventing a sufficient amount of less abundant proteins ...

Enzyme Explorer Assays by Enzyme Name K - P

Sorted by: EC Number Enzyme Activator Assays Protease Inhibitor Assays Non-Specific Protease Assays Protein Quantitation Assays Quantitative Metabolite Assays - A -, - B -, - C -, - D -, - E -, - F -, - G -, - H -, - I -, - K -, - L -, - M -, - N -, - O -, - P -, - R -, - S -, - T -, - U -, - X - - ...

Professor Product Portal - Andrew Wilson Group

Related Links • VIEW PRODUCT LIST • Professor Product Portal • Organic Synthetic Chemistry Professor Product Portal Index Professor Andrew Wilson The Wilson group use innovative synthetic methods to develop small molecule mimics and probes of biomacromolecule structure and function. A particular foc...

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the cell culture vessel, have proven to be efficient methods to better mimic in vivo cell behavior. We de...

Anti-FLAG® M2 Magnetic Beads

• Anti-FLAG® M2 Magnetic Beads for FLAG® Tag Protein Capture • Sample Preparation and Affinity Purification • Electrophoresis and Western Blotting • Comparison of Elution Techniques for FLAG-tagged Fusion Proteins • FLAG® Tag Antibodies, Control Proteins, and Affinity Purification Tools Anti-FLAG® M...

Dulbecco's Modified Eagle's Medium (DME) Formulation

Many composition modifications of Eagle's Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco's Modified Eagle's medium (DMEM). DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold...

Formulation of Biomolecules

We offer a wide range of high-quality stabilizers, buffers and salts to successfully purify and formulate your biomolecules. Chemicals play an important role in the stabilization of a biologic drug during its manufacturing and formulation process – for instance, by preventing aggregation. Exemplary ...

Bioactive Small Molecules Literature References

Looking for current application references for Bioactive Small Molecules? Look no further! • Apoptosis • Kinase Phosphatase Biology • Neuroscience Apoptosis Publications: Click on the title to view the abstract on PubMed. Title Author / Journal Product Cited The retinoblastoma protein regulates peri...

Enzymatic Assay and ATP Sensitivity Test of Luciferase

To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase. This scope of this procedure applies to products that have a specification for enzymatic activity of Luciferase and/or a specification for ATP sensitivity. 3.1 ATP ...

LUCY® Stains - fluorescence dyes for protein gel stains

LUCY Protein Stain Product List Three fluorescent protein stains, LUCY-506, LUCY-565 and LUCY-569 have been developed. These dyes are suitable for staining proteins on polyacrylamide gels (both 1D- and 2D-Electrophoresis) and offer a reliable, convenient and economic alternative to current silver st...

Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

Sample extraction procedures often release proteases into solution, requiring the addition of protease inhibitors to prevent unwanted proteolysis. An alternative to the addition of inhibitors is to use a group-specific AC medium to remove the proteases from the sample. The same procedure can be used ...

Maruoka Catalysts

Asymmetric phase transfer catalysis using the Maruoka catalysts has proven to be an ideal method for the enantioselective preparation of natural and unnatural α-alkyl and α,α-dialkyl-α-amino acids from glycine derivatives. While various related catalysts have been developed for this type of transfor...

Physical Stability of a Gel Filtration Column for Antibody Analysis

Independent of the mode of liquid chromatography, the lifetime of an HPLC or UHPLC column depends on the characteristics of the packing material, how that material was packed into the column and how the column was subsequently used by the operator. Clearly, when the structure of the packed column li...

Mitochondrial Stress and ROS

Oxidative stress is implicated in the pathogenesis of lipotoxicity in both animal and human studies. Chronic oxidative stress is linked to insulin resistance in multiple tissues. Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled ...

Peptide Upload

What's available • Defining the Peptide Type • Defining the Delimiter • D-Form Amino Acid Option • Tab Delimited Data • Comma Delimited Data • Element Codes • Sequence Codes • Packaging Options • What happens after you click "Price"? Defining the Peptide Type To get started using the copy/paste func...

Bile Acids Kit for LC-MS

A Rapid and Reliable Kit for Bile Acids Research Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes. BA analysis is an important area of study in various settings including clinical ...

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

• • Download PDF • Immunoblotting (Western blot transfer) is a common technique in modern proteomics research. After electrophoresis, proteins are immobilized on a nitrocellulose or PVDF membrane and then probed with a primary antibody against the protein-of-interest. A secondary antibody conjugated...

Collagen

Collagen is the most abundant mammalian protein and the main component of connective tissues. Typically collagen is first synthesized as individual glycine-rich preprocollagen monomeric strands from the ribosomes in the rough endoplasmic reticulum (RER). After transport to the lumen of the RER signa...

Biodegradable Aliphatic Polyesters for Drug Delivery

1Department of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, TX 75080 2Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080 *Email: mihaela@utdallas.edu Innovations in polymer technology have had a significant impact on the advancement of novel d...

Automated IgG Purification

Related Links • PureProteome™ Protein A Magnetic Beads • PureProteome™ Protein G Magnetic Beads • SNAP® i.d. 2.0 immunodetection system • Immobilon®-E PVDF transfer membranes • Enhanced Chemiluminescent (ECL) Substrates Related Products • Protein Sample Prep • Western Blotting • Proteomics As the de...