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172 matches found for NaOH

NaOH - Sodium Hydroxide

What is sodium hydroxide?NaOH is a strongly basic versatile inorganic compound often referred to as caustic soda, lye, or sodium hydrate. This common laboratory alkali is used for general cleaning of equipment, buffer solutions, and titrations, among other uses. In the home, NaOH is found in soaps, ...

NIOSH 7605– Products and Materials Guide

CHROMIUM, HEXAVALENT by Ion Chromatography This method can be used for the determination of Cr(VI) using 2% NaOH - 3%Na2CO3for extraction. All sample preparation should be performed in a hood. Chromium Hexavalent are chemical compounds that contain choromium in the +6 oxidation state. Hexavalent chr...

NIOSH 7401– Products and Materials Guide

Alkaline Dusts This method was was validated using generated atmospheres of NaOH over the range 0.76 to 3.9 mg/m3 using a 360-L sample. The NaOH concentration was independently verified by analyzing additional samples for sodium by atomic absorption spectrophotometry. An overall average collection e...

Blocking Reagent Protocol & Troubleshooting

Product No. 11096176001 Preparation of stock solution (10x): Blocking Reagent is dissolved in Maleic acid buffer [100 mM Maleic acid, 250 mM NaCl, pH 7.5 (20 °C) adjust with conc. or solid NaOH], to a final concentration of 10% (w/v) with shaking and heating either on a heating block to +60 °C for a...

Slow-Release of Unstable Boronic Acids from MIDA Boronates

Aaron Thornton, Ph.D. Product Manager aaron.thornton@sial.com In addition to attenuated reactivity towards anhydrous cross-coupling conditions, MIDA boronates also possess the capacity for in situ slowrelease of boronic acids under aqueous basic conditions (Scheme 1). Harnessing this phenomenon, bor...

MIDA Boronates - Boronic Acids and Derivatives

MIDA boronates represent a new class of caged boronic acids and have proven exceptionally successful in iterative Suzuki-Miyaura cross-couplings. These boronic acid surrogates attenuate the transmetallation between the boronic acid and palladium species; however, deprotection is readily achieved at ...

Assay Procedure for Protease

The appearance of peptides is measured as tyrosine equivalent at 275nm by spectrophotomertry. One unit causes the increase of optical density at 275nm corresponding to one micromoloe of tyrosine per minute under the conditions described below. Reagents A. Casein solution :0.6% [Suspend 3.0g of Hamme...

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.7) One unit causes the formation of one milligram of reducing sugars equivalent to the glucose at 3 minutes under the conditions described below (This activity is equivalent to the international unit that hy...

FLAG IP Detect Kit

Immunoprecipitation Detection Kit (Product Code COIP-D) The Immunoprecipitation Detection Kit provides all of the unique reagents required to detect protein-protein interactions using a plate-based colorimetric assay. The substrate p-Nitrophenyl Phosphate Disodium (pNPP) is hydrolyzed by Anti-c-Myc ...

Assay Procedure for Creatininase

The appearance of creatinine-picrate (orange dye based on Jaffe's reaction) is measured at 520nm by spectrophotometry. One unit causes the formation of one micromole of orange dye per minute under the conditions described below. Reagents A. Creatine solution :0.1M[1.49g creatine /100ml of 50mM phosp...

Assay Procedure for L-Glutamic Dehydrogenase (NADP)

The disappearance of NADPH is measured at 340nm by spectrophotometry. One unit causes the oxidation of one micromole of NADPH per minute under the conditions described below. Reagents A. Buffer solution :0.1M Tris-HCl buffer, pH 8.3 B. NH4Cl solution :3.3M C. α-Ketoglutarate solution 0.225M (adjust ...

Small-Scale Isolation of pGEX DNA

Rapid and phenol-free isolation of plasmid DNA is greatly simplified by the use of illustra plasmidPrep Midi Flow Kit or illustra plasmidPrep Mini Spin Kit. An alternate procedure is described below. Reagents required Solution I: 100 mM Tris-HCl, pH 7.5, 10 mM EDTA, 400 µg of heat-treated RNase I pe...

Asymmetric Reduction of Ketones

ChemFiles 2008, 8.6, 5. The enantioselective reduction of ketones to alcohols gives access to a pool of chiral building blocks that can be used for the synthesis of natural products. Adolfsson and co-workers reported a novel class of ligands, based on a pseudo-dipeptide,1 for the mild and efficient ...

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing medium in concentrated form is not recommended as precipitates may form. Supplements can be added prio...

Concentrations of Common Reagents Chart

Composition of concentrated reagent grade acids, ammonium hydroxide, and sodium and potassium hydroxide solutions (with dilution directions to prepare 1N solution) Chemical Name Molecular Formula Approx. Strength of Concd. Reagenta Molarity of Concd. Reagent Milliliters of Concd. Reagent Necessary t...

Aldol Condensation Reaction

The aldol condensation reaction is an organic reaction introduced by Charles Wurtz, who first prepared the β-hydroxy aldehyde from acetaldehdye in 1872.1 In an aldol condensation, an enolate ion reacts with a carbonyl compound in the presence of acid/base catalyst to form a β-hydroxy aldehyde or β-h...

Assay Procedure for Lipase

The appearance of quinoneimine dye is measured at 545nm by spectrophotometry. One unit causes the formation of one micromole of glycerol (half a micromole of quinoneimine dye) per minute under the conditions described below. Reagents A. Olive oil emulsion :Sonicate the mixture of 5.0g of olive oil[r...

Maximize recombinant protein yields with ISOGRO®

• Substantially increase recombinant protein expression levels using ISOGRO as a stand-alone media versus M9 media. • Save time and money by using ISOGRO growth media to shorten production time. • Stable isotope label sufficient amounts of protein to permit the achievement of high quality NMR spectr...

Catalytic Direct Cross-Coupling of Organolithium Compounds with Aryl Chlorides

Complex, hindered biaryls have been prepared at temperatures ranging from 0�C to room temperature, or with gentle heating. The Pd-PEPPSI-IPent catalyst nicely couples starting materials containing acidic moieties and routinely prepares biaryl derivatives where one or both rings comprising the biaryl...

Enzymatic Assay of Collagenase

This procedure may be used for Collagenase products. The continuous spectrophotometric rate determination (A345, Light path = 1 cm) is based on the following reaction: where: FALGPA = N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala FAL = N-(3[2-Furyl]acryloyl)-Leu Unit Definition – One unit of Collagenase h...

Kjeldahl Method Standard

Michaela Buffler, Product Specialist Kjeldahl Solutions, Büchi Labortechnik AG, Meierseggstr. 40, CH-9230 Flawil, Switzerland buffler.m@buchi.com Nicole Amann, Product Manager Analytical Standards For almost 130 years the determination of nitrogen using the method developed by Danish chemist Johan G...

Sample Preparation for Ettan DIGE System Applications

CyDye™ DIGE Fluor minimal dyes, used for protein labeling in Ettan DIGE system applications, form a peptide linkage between the fluor and lysine residues within the protein. Components such as primary amines (e.g. ampholytes) will compete with the proteins for fluor binding. Thiols (e.g. DTT) also c...

Enzymatic Assay of Protease Using Casein As a Substrate

• • Download PDF • To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis. P4630, P4755, P0384, P5380, P7431, P6141, P1512, P9040, P5147, P5647, P8775, P7026, P4032, P8038, P8298, P2789, P4789, P6670, P3910, P4806, and any specified impu...

ELISA Procedures

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool. • Indirect ELISA • Capture ELISA • Troubleshooting Guide (PDF) • References • Reagents and Equipment • Procedure Reagents and Equipment 1. Phosphate buffered saline (PBS) tablet: 10 mM phosphate buf...

Oxazolidinone Chiral Auxiliaries in Chemical Sythesis

• High Diastereoselectivity • Crystalline Products • High Organic Solubility • Mild Hydrolysis • Higher Temperature Acylation • Easier to Recycle Products Reaction Schemes Oxazolidinone chiral auxiliaries are often used for diastereoselective Michael additions, alkylations, aldol condensations, cycl...

KitAlysis™ High-Throughput Base Reaction Screening Kit

Contents in each of the 2 individually sealed Mylar (foil bags): • 22 (11 x 2) pre-weighed bases in glass vials loaded with stir bars, topped with cap mat. • 4 empty 4 mL reaction vials The screening sets come pre-loaded with 30 µmol of base in each vial according to the following design. Mouse over...

Cotinine Determination by HPLC RP Separation Coupled to UV Detection Using Ascentis™ C18 Column

Pasquale Moio and Andrea Urbani, Centro Studi sull’Invecchiamento (Ce.S.I.), Fondazione Università “G. D’Annunzio”, Chieti, Italy and Dipartimento di Scienze Biomediche, Università “G. D’Annunzio” di Chieti e Pescara, Italy. Email: a.urbani@unich.it, pmoio@unich.it Tobacco smoking elicits a complex ...

Enzymatic Assay Of Alcohol Dehydrogenase Attached To Agarose (A2529)

The objective of this procedure is to standardize the enzymatic assay of Alcohol Dehydrogenase attached to Agarose, Product Number (A2529), at our R&D center St. Louis. The scope of this procedure includes activity assays of Alcohol Dehydrogenase attached to Agarose, Product Number (A2529). One unit...

Direct and Crude Extract qPCR

Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE FORCE qPCR Kits and KAPA Express Extract Enzymatic inhibition, caused by both sample and extraction carryover inhibitors, is a significant obstacle when optimizing crude sample workflows. KAPA PROBE FORCE q...

Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA? Benzonase Nuclease is a promiscuous endonuclease that attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular). For what purposes would I want to use Benzonase N...