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GC Analysis of Ibuprofen Enantiomers (Underivatized) on β-DEX™ 120

column Supelco beta-DEX 120, 30 m x 0.25 mm I.D., 0.25 µm (24304) carrier gas helium, 60 m/sec., constant flow gradient 50 °C (5 min), 5 °C/min to 200 °C (10 min) sample 2 mg/mL in methanolpeak 1: S(+)-ibuprofenpeak 2: R(-)-ibuprofen injection 1 µL, 100:1 split liner 4 mm I.D., FocusLiner™ inj. temp...

Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

In many cases, an exact knowledge of protein quantities is required for protein chemistry applications. Amino acid analysis enables precise determination of protein quantities and provides detailed information regarding the relative amino acid composition and free amino acids. The relative amino aci...

HPLC Analysis of 1-Aminoindan Enantiomers on LARIHC™ CF6-P

column LARIHC CF6-P, 25 cm x 4.6 mm I.D., 5 µm particles (AZYP Part No.L1001, available from Supelco/Sigma-Aldrich as a custom item) column temp. 20 °C mobile phase [A] methanol: [B] acetonitrile: [C] acetic acid: [D] triethylamine, (70:30:0.3.0.2, A:B:C:D) flow rate 1 mL/min sample 1-Aminoindan, 0....

HPLC Analysis of Octopamine Enantiomers on LARIHC™ CF6-P

column LARIHC CF6-P, 25 cm x 4.6 mm I.D., 5 µm particles (AZYP Part No.L1001, available from Supelco/Sigma-Aldrich as a custom item.) column temp. 20 °C mobile phase [A] methanol: [B] acetonitrile: [C] acetic acid: [D] triethylamine, (70:30:0.3.0.2, A:B:C:D) flow rate 1 mL/min sample Octopamine, 0.3...

Selecting a GC Column for Specific Industries/Applications

We know that only the most accurate analytical products will do. That’s why we offer the Supelco portfolio of analytical GC columns, providing you the accuracy, precision and consistency you need. Gas chromatography, first established in the 1950′s, is a mature analytical technique with many establi...

HPLC Analysis of trans-1-Amino-2-Indanol Enantiomers on LARIHC™ CF6-P

column LARIHC CF6-P, 25 cm x 4.6 mm I.D., 5 µm particles (AZYP Part No.L1001, available from Supelco/Sigma-Aldrich as a custom item.) column temp. 20 °C mobile phase [A] methanol; [B] acetonitrile; [C] acetic acid; [D] triethylamine, (70:30:0.3.0.2, A:B:C:D) flow rate 1 mL/min sample Trans-1-Amino-2...

LC/MS Analysis of 1,3-Dimethylamylamine (DMAA) in Natural and Spiked Geranium Oil on AZYP LARIHC™ CF6-P

column AZYP LARIHC CF6-P, 15 cm x 2.1 mm I.D., 5 µm particles (Available from Supelco, Please inquire) mobile phase [A] acetonitrile; [B] methanol; [C] formic acid; (90:10:0.1, A:B:C) flow rate 0.1 mL/min sample Geranium oil, natural and spiked with 100 ppb synthetic DMAA detector MS, ESI-LIT(+), SI...

HPLC Analysis of Phenylalanine Methyl Ester Enantiomers on LARIHC™ CF6-P

column LARIHC CF6-P, 25 cm x 4.6 mm I.D., 5 µm particles (AZYP Part No.L1001, available from Supelco/Sigma-Aldrich as a custom item) column temp. 20 °C mobile phase [A] methanol: [B] acetonitrile: [C] acetic acid: [D] triethylamine, (70:30:0.3:0.2. A:B:C:D) flow rate 1 mL/min sample Phenylalanine me...

GC Analysis of Fragrance Compounds in Shampoo Base on a 100% Methyl Column after SPME using 100 μm PDMS Fiber

desorption process 5 min, 250 °C desorption temp. -50 °C w/liquid CO2 extraction headspace, 5 min, 80 °C (with sample stirring) SPME fiber polydimethylsiloxane, 100 µm film (57300-U) column 100% methyl polysiloxane, 50 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, Equity-1, 28047-U) oven 35 °C to 2...

GC Analysis of Phenols in Water on a 5% Phenyl Column after SPME using 85 μm Polyacrylate Fiber

sample/matrix 50 ppb phenols in water, 27% NaCl, pH 2 desorption process splitless, 280 °C (closed 3 min) extraction 20 min immersed in water with rapid stirring SPME fiber polyacrylate, 85 µm (57304) column 5% Phenyl Polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, SLB-5ms, 28471-U) ...

GC Analysis of a 38-Component FAME Mix on SP™-2560, 200 m Column

column SP-2560, 200 m x 0.25 mm I.D., 0.20 µm (29688-U) oven 100 °C (13 min), 4 °C/min to 240 °C (30 min) carrier gas hydrogen, 27 cm/sec sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME injection 1.0 µL, 50:1 split liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID,...

GC Analysis of a 38-Component FAME Mix on SLB®-IL111, 200 m Column

column SLB-IL111, 200 m x 0.25 mm I.D., 0.20 µm (29689-U) oven 100 °C (13 min), 4 °C/min to 240 °C (30 min) carrier gas hydrogen, 27 cm/sec sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME injection 1.0 µL, 50:1 split liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FI...

GC Analysis of a 38-Component Fatty Acid Methyl Ester (FAME) Mix on SLB®-IL60, Comparison to a Polyethylene Glycol (PEG) Column

column SLB-IL60, 30 m x 0.25 mm I.D., 0.20 µm (29505-U) column PEG 3, 30 m x 0.25 mm I.D., 0.25 µm oven 170 °C, 1 °C/min to 225 °C carrier gas helium, 1.2 mL/min sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3, in methylene chloride liner 4 mm I.D., split/splitless type, single taper wool p...

GC Analysis of Semivolatiles in Water on a 5% Phenyl Column after SPME using 7 μm PDMS

sample/matrix water spiked with PAHs and phthalates at 50 ppb extraction direct immersion in water, 15 min (rapid stirring) SPME fiber polydimethylsiloxane, 7 µm (57302) column 5% Phenyl Polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, SLB-5ms, 28471-U) oven 60 °C (3 min), 10 °C/min t...

GC Analysis of Gasoline Residue in Arson Samples on a 100% Methyl Column after SPME using 100 μm PDMS Fiber

SPME fiber polydimethylsiloxane phase fiber, 100 µm headspace sampling (20 min) 10 sec desorption (splitless mode) (57300-U) column 100% methyl polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, Equity-1, 28046-U) oven 35 °C (2 min) to 220 °C at 10 °C/min, hold 2 min, to 300 °C at 30 °C...

GC Analysis of a 37-Component FAME Mix on Omegawax®

column Omegawax 250, 30 m x 0.25 mm I.D., 0.25 µm (24136) oven 50 °C, 3.0 °C/min to 280 °C carrier gas helium, 40 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL50:1 split inj. temp. 240 °C detector FID, 280 °C...

GC Analysis of Explosives in Water on a 14% Cyanopropylphenyl Column after SPME using 65 μm PDMS/DVB Fiber

sample/matrix 50 ng/mL each explosive in water desorption process 5 min, 250 °C extraction immersion, 30 min SPME fiber polydimethylsiloxane/divinylbenzene, 65 μm (57310-U) column 14% cyanopropylphenyl polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, Equity-1701, 28372-U) oven 95 °C (...

GC Analysis of a 37-Component FAME Mix on Equity®-1 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

column Equity-1, 15 m x 0.10 mm I.D., 0.10 µm (28039-U) oven 100 °C, 50 °C/min to 300 °C (1 min) carrier gas hydrogen, 50 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 0.2 µL, 200:1 split liner 4 mm I.D., split, c...

GC Analysis of Chemical Signals from Spruce Seedlings (Healthy vs. Wounded) on a 5%Phenyl Column after SPME using 100 μm PDMS Fiber

sample/matrix headspace surrounding Picea abies seedlings desorption process 225 °C for 30 sec extraction headspace, 60 min. SPME fiber 100 µm polydimethylsiloxane (57300-U) column 5% phenyl polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, Equity-5, 28089-U) oven 40 °C (4 min), 4 °C/m...

GC Analysis of a 37-Component FAME Mix on SLB®-IL111

column SLB-IL111, 100 m x 0.25 mm I.D., 0.20 µm (29647-U) oven 140 °C (5 min), 8 °C/min to 180 °C, 5 °C/min to 260 °C carrier gas hydrogen, 40 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 100:1 split liner 4 mm I.D....

GC Analysis of Diphenylamine and Methoxychlor in Apples on a 14%Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb

sample preparation SPE (Solid Phase Extraction) sample/matrix homogenize 50 g sample in 100 mL acetonitrile then add 10 g NaCl and homogenize 5 min SPE tube/cartridge ENVI-Carb, 500 mg/6 mL column 14% cyanopropylphenyl polysiloxane, 30 m x 0.25 mm I.D., 0.15 µm (Supelco equivalent, Equity-1701, 2837...

Ionic Liquid GC Capillary Columns

We know that only the most accurate analytical products will do. That’s why we offer the only portfolio of Ionic Liquid GC columns under the Supelco brand, providing you the accuracy, precision and consistency you need. In 2005, Prof. Daniel W. Armstrong (University of Texas at Arlington) showed tha...

GC Analysis of a 37-Component FAME Mix on SPB-PUFA

column SPB-PUFA, 30 m x 0.32 mm I.D., 0.20 µm (24323) oven 50 °C (2 min), 4 °C/min to 210 °C carrier gas helium, 25 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 100:1 split inj. temp. 250 °C detector FID, 260 °C Cat...

GC Analysis of a 37-Component FAME Mix on Omegawax® (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

column Omegawax 100, 15 m x 0.10 mm I.D., 0.10 µm (23399-U) oven 140 °C, 40 °C/min to 280 °C (2 min) carrier gas hydrogen, 50 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 0.2 µL, 200:1 split liner 4 mm I.D., spli...

GC Analysis of a 37-Component FAME Mix on SLB®-IL100

column SLB-IL100, 30 m x 0.25 mm I.D., 0.20 µm (28884-U) oven 50 °C, 3.0 °C/min to 240 °C carrier gas helium, 40 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL50:1 split inj. temp. 240 °C detector FID, 240 °C ...

GC Analysis of a 37-Component FAME Mix on SP™-2560

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 140 °C (5 min), 8 °C/min to 180 °C, 4 °C/min to 210 °C, 20 °C/min to 250 °C (7 min) carrier gas hydrogen, 40 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 10...

GC Analysis of Organophosphorus Pesticides (OP-Pest) on a 5% Phenyl Column after SPME using 100 μm PDMS Fiber

sample/matrix 50 ppb each in 1.5 mL saturated salt water, pH 7.2 extraction 20 min immersion with rapid stirring SPME fiber polydimethylsiloxane, 100 µm (57300-U) column 5% phenyl polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, SLB-5ms, 28471-U) oven 60 °C (1 min) to 300 °C at 12 °C/...

GC Analysis of Cocaine in Urine on a 100%Methyl Column after SPME using 100 μm PDMS Fiber

sample/matrix 0.5 mL urine (250 ng each analyte, 20 µL 2.5% NaF) in 1 mL vial desorption process 3 min, 240 °C extraction immersion, 30 min SPME fiber polydimethylsiloxane, 100 µm (57300-U) column 100% methyl polysiloxane, 30 m x 0.32 mm I.D., 0.25 µm (Supelco equivalent, Equity-1, 28055-U) oven 120...

Selecting a GC Column for Specific Methods

Related Links • GC analysis • GC Workflow tools • GC Sample preparation • Selecting a GC Column for Specific Industries/Applications • Selecting a GC Column by a Specific Stationary Phase • Capillary GC Columns for GC x GC • Ionic liquid capillary and SLB® i-series columns • Application/Chromatogram...

OSHA Method 52: GC Analysis of Formaldehyde on SUPELCOWAX® 10 after Collection/Desorption using ORBO™-24

sample/matrix HMP-coated Supelpak 20 spiked with formaldehyde (10 mug) adsorbent tube ORBO-24 (20231) column SUPELCOWAX 10, 30m x 0.53 mm I.D., 0.5 µm (25325) oven 50 °C (2 min) to 200 °C at 10 °C/min carrier gas helium, 7 mL/min injection 1 µLdirect, 220 °C detector NPD, 220 °C Categories Analytica...