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136 matches found for ammonium sulphate

Ammonium Sulfate Suspensions - Technical Note

— If the ammonium sulfate might interfere with the reaction(s): 1. Remove the enzyme from the refrigerator. Note: We strongly recommend that ammonium sulfate suspensions not be frozen. 2. Make sure the cap is on tight, and then invert the vial/bottle several times to provide a milky suspension. This...

Fractional Precipitation of Affinity Chromatography Samples

Fractional precipitation is frequently used at laboratory scale to remove gross impurities from small sample volumes, and occasionally used in small-scale commercial production. Precipitation techniques separate fractions by the principle of differential solubility. Because protein species differ in...

Kjeldahl Method Standard

Michaela Buffler, Product Specialist Kjeldahl Solutions, Büchi Labortechnik AG, Meierseggstr. 40, CH-9230 Flawil, Switzerland buffler.m@buchi.com Nicole Amann, Product Manager Analytical Standards For almost 130 years the determination of nitrogen using the method developed by Danish chemist Johan G...

Sample Preparation in Ion Exchange Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, may reduce the need for stringent washing procedures and can extend the life of the chromatographic medium. Sample...

Performing a Separation with HiTrap IgM Purifcation HP

Related Links • Fractional Precipitation of Affinity Chromatography Samples • Removal of Lipoproteins from Affinity Chromatography Samples • Sample Preparation for Affinity Chromatography • Column Packing and Preparation for Affinity Chromatography • Converting From Linear Fow (cm/hour) to Volumetri...

Detergent Properties and Applications

Related Links • All About Detergents • Detergent Handbook • Detergent Poster • Bile Acids • Membrane Proteins Categories Detergents by Application • Anti-Foaming Agents • Antigen-Vaccine Preparation • Cell Culture • Cell Lysis • Diagnostic Applications • Drug Delivery-Liposomes • Electrophoresis Chr...

Glycolytic Enzymes

Product Name Product # a-N-Acetylgalactosaminidase from chicken liver, Lyophilized powder, 5-20 units/mg protein A9763 b-N-Acetylglucosaminidase A preparation from bovine epididymis, Ammonium sulfate suspension, 0.25-1.5 units/mg protein (modified Warburg-Christian) A3391 b-N-Acetylglucosaminidase B...

Solubility Table of Compounds in Water at Temperature

Related Resources Compound Name Chemical Formula Solubility in g/100 g H2O at °C Content of the total solution at 20°C in % Density of the total solution at 20°C in % 0 20 40 60 80 100 Aluminum ammonium sulfate AlNH4(SO4)2 · 12H2O 2.6 6.59 12.36 21.1 35.2 109.2 (95°C) 6.18 1.0459 (15.5°C) dodecahydr...

Streptavidin Mutein Matrix Troubleshooting

Product No. 03708152001 Biotinylated protein appears in flow-through Column overloaded Reduce sample load. Binding conditions were not optimal In some cases, monobiotinylated proteins may require a higher salt concentration to bind to the mutant streptavidin. Increase ammonium sulfate concentration ...

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximum sensitivity. • Fixing Solution (F7264) • Acetic Acid, Glacial (A6283) • Methanol (M3641) • Ammonium...

Salt Selection and Buffer Preparation

In HIC the binding process is more selective than the elution process, so it is essential to optimize the conditions of the start buffer. The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize co...

Strat-M® Membrane Chemical Compatibility

Follow these recommended chemical compatibility guidelines when testing formulations and solutions using a Strat-M® membrane. R = Recommended N = Not Recommended L = Limited Applications (test) Solvents Strat-M® Membrane Solvents Strat-M® Membrane Acetone N Guanidine Thiocyanate (5M) R Acetonitrile ...

Stable Labeled Minimal Media and Minimal Media Components for Biomolecular NMR

The use of E.coli in the expression of isotopically enriched recombinant proteins for NMR studies has remained the most popular technique to date. The prevalence of this method is due to the availability of minimal media as the source of the isotopic label(s). Minimal media has been and continues t...

Orchid Culture Media Formulations

Related Links • Plant Tissue Culture Products • Media Preparation • Media Sterilization • Orchid Seed Sterilization Orchid tissue culture has its own unique requirements. Below is formulation information for a number of media that are used to successfully culture Orchidaceae. K4003 - Knudson C modif...

Crude Collagenase For General Use - Collagenases

Crude collagenase preparations come from the culture medium of Clostridium histolyticum, an anaerobic, gram-positive bacterium, commonly found in soil. In addition to collagenase, the culture medium contains as many as six proteases, including clostripain, a caseinase, and a tryptic-like proteinase...

Rapid, Ultrafiltration-based Method for Purification of Monoclonal Antibodies Using the Centricon® Plus-70 Device

Monoclonal antibodies (MAb) continue to gain importance as therapeutic and diagnostic agents for many cancers. The process of screening hybridoma libraries for candidate MAbs is both time consuming and labor intensive. Once a hybridoma cell line expressing the desired MAb is established, a bench-sca...

Enzyme Class Index: Hydrolases on esters

We offer over 1,600 enzymes as well as associated inhibitors and substrates. TO SEARCH FOR AN ENZYME BY EC # OR REACTION CATALYZED... 1. Click on a category in the menu below 2. Scroll down the list to the enzyme of interest 3. Click on the Product # to access product listing and related information...

Murashige and Skoog Media Formulations

Related Links • Plant Tissue Culture Products • Media Preparation • Media Sterilization Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media. • Basal Salts Mixtures • Medi...

FAQ Videos Spectroquant® USEPA Methods Equivalency

Related Products • USEPA • Safe Drinking Water Act & Clean Water Act • Request USEPA Documents • Spectroquant® Prove Family • Spectroquant® Test Kits A–Z • All Spectroquant® Kits • Spectroquant® Prove Connect • Diluted, Certified Standard Solutions (CRM) • Test Strips and Visual Tests • Water, Food ...

Lab Chemicals

Analytical Lab Products | Biomonitoring Products | Life Science Products Lab Chemicals Product No. Description 900522 Toluene anhydrous, ZerO2®, 99.8% 298964 N,N′-Diisopropylethylenediamine 99% 402729 2-Methyl-5-nitroaniline 95% 244554 Barium chlorate monohydrate 98% 270245 4-Methoxybenzyl chloride ...

DMEM/nutrient Mixture F-12 Formulation

Click on the product numbers at the top of each column. Component D0547 g/L D2906 g/L D6421 [1x] g/L D6434 [1x] g/L D8062 [1x] g/L D8437 [1x] g/L D8900 g/L D9785 g/L Inorganic Salts Ammonium Molybdate • 4H2O 0.00000618 — — — — — — — Ammonium Metavandate 0.00000058 — — — — — — — Calcium Chloride 0.11...

Common Cell Culture Problems: Precipitates- Temperature shift

Related Links • Troubleshooting Guide Home • Contamination • Poor Cell Growth • Poor Cell Attachment • Cell Clumping • Cell Death • Cell Line Misidentification • Preventing Cell Culture Contamination (video) • Cell Culture Related Products • Copper(II) chloride dihydrate powder • apo-Transferrin • I...

Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA? Benzonase Nuclease is a promiscuous endonuclease that attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular). For what purposes would I want to use Benzonase N...

Screening for Selectivity of HIC Medium

Fig 19. HiTrap™ HIC Selection Kit and RESOURCE™ HIC Test Kit. Time and sample can be saved in the early stages of development by using small (1 ml), prepacked columns such as those in the HiTrap™ HIC Selection Kit and RESOURCE™ HIC Test Kit. Media can be quickly and efficiently screened for the requ...

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today. Below, find formulation information for these commonly used media. Product No. Description C1416 Chu (N6) basal salt mixt...

Precipitation Procedures

Protein precipitation is an optional step in sample preparation for 2-D electrophoresis. Precipitation, followed by resuspension in sample solution, is generally employed to selectively separate proteins in the sample from contaminating species such as salts, detergents, nucleic acids, lipids, etc.,...

Performing a Separation with Sepharose® High Performance: Purification with High Resolution

Correct sample and buffer preparation is essential in order to achieve optimal separation and avoid any deterioration in column performance. Samples must be fully dissolved and free from particles or other material likely to interfere with the separation. Refer to Appendix 1 for recommendations and ...

Storage of Biological Samples for Affinity Chromatography

The advice given here is of a general nature and cannot be applied to every biological sample. Always consider the properties of the specific sample and its intended use before following any of these recommendations. • Add stabilizing agents, when necessary. Stabilizing agents are often required for ...

Multiplex Quantification of Infliximab and Adalimumab

Related Products • LC-MS solutions • Sample Preparation • HPLC and UHPLC columns • UHPLC solvents Infliximab and Adalimumab monoclonal antibodies (mAbs) are used widely to treat rheumatoid arthritis, psoriatic arthritis and many autoimmune diseases by binding to tumor necrosis factor-alpha (TNFα) to...

Trypsin Inhibitors

Trypsin Inhibitor Products Cell Culture Application Trypsin Inhibitor Assay Procedure Natural trypsin Inhibitors also known as serine protease inhibitors (serpins) are the largest and most diverse family of protease inhibitors.1 Serpins control the activation and catabolism of proteins by the inhibi...