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67 matches found for barbital

B8632-99h88041 - Datasheet (62KB)

BARBITAL Methanol Solution Product Number B 8632 Lot Number 99H88041 CAS Number [57-44-3] Concentration 1.02 mg Barbital/mL GC grade methanol (conc. verified by UV) Molecular Formula C 8H12N2O3 Molecular Weight 184.2 DEA Class Exempt preparation of Class IV Synonyms Barbitone, Malonal, Diethylmalony...

B5934 - Datasheet (22 KB)

Barbital Buffer Product Number B 5934 Store at Room Temperature Product Description Barbital Buffer is a general purpose electrophoresis buffer for separation of serum proteins, lipoproteins, and isoenzymes such as lactate dehydrogenase and creatine kinase. The buffer provides excellent resolution o...

Appl report 217 (15 KB )

sigma-aldrich.com/supelco Application Report 217 Analysis of Barbitals Using Ascentis ™ RP-Amide This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of seven Barbitals. Structures along with the optimized chromatogram obtained on the Ascentis RP-Amide are presente...

Appl report 216 (15 KB )

sigma-aldrich.com/supelco Application Report 216 Analysis of Barbitals Using Ascentis ™ C18 This application demonstrates the suitability of the Ascentis C18 for the analysis of seven Barbitals. Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented. Key Words Ba...

Bulletin 844 (154 KB )

SUPELCO Bulletin 844A 1 Bulletin 844A Postcolumn Reactions Enhance Detection Sensitivity and Selectivity in HPLC Analyses Figure A. Barbitals With and Without Photochemical Reactor Enhancement Detection Column: SUPELCOSIL LC-18, 25cm x 4.6mm ID, 5µm particles Cat. No.: 58298 Mobile Phase: 0.02M NaH ...

A5304 - Datasheet (21 KB)

AGAROSE High EEO Product Number A 5304 Store at Room Temperature Procedure Preparation and Use of Counter Immunoelectrophoresis Gels 1. Dissolve one vial Barbital Buffer (Product No. B 6632) in one liter deionized water. 2. Prepare 30 ml of 1% agarose solution (sufficient for two 85 x 100 mm gels) b...

Nuclease P1 (17 KB)

Revised: 09/08/94 Page 1 of 3 Enzymatic Assay of NUCLEASE P1 (EC 3.1.30.1) RNA as Substrate PRINCIPLE: Nuclease P1 RNA + H2O + > Acid Soluble Polynucleotides Abbreviation used: RNA = Ribonucleic Acid CONDITIONS: T = 37°C, pH = 5.3, A260nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Deter...

T9010 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T9681 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T4265 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T0553 - Enzyme Assay (24 KB)

T0553 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

T7009 - Enzyme Assay (24 KB)

T7009 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

T7513 - Enzyme Assay (24 KB)

T7513 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

Appl report 342 (15 KB )

sigma-aldrich.com/supelco Application Report 342 Analysis of Barbituates LC-UV Using Ascentis ™ C8 Seven barbituates are separated on Ascentis C8 and detected by UV at 214 nm. Key Words LC-UV , Ascentis C8, drugs of abuse, 34966, 39253, 57-44-3, B-0375, 50-06-6, P-1636, 76-73-3, S-1503, 57-33-0, P-3...

T3399 - Enzyme Assay (54 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T8885 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T9135 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

Hirudin (15 KB)

BEPLAS03 Page 1 of 4 Revised: 03/16/98 Enzymatic Assay of HIRUDIN 1 PRINCIPLE: Hirudin + Thrombin > Hirudin/Thrombin complex + Thrombin (excess) Plasma (Fibrinogen) Thrombin (excess)> Clot (Fibrin) CONDITIONS: T = 37°C, pH = 7.35 METHOD: Fibrometer A. 36 mM Diethyl Barbiturate and 36 mM Sodium Aceta...

T6884 - Enzyme Assay (24 KB)

T6884 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

Ancrod (17 KB)

Revised: 01/05/96 Page 1 of 4 Enzymatic Assay of ANCROD (EC 3.4.21.28) PRINCIPLE: Fibrinogen Ancrod > Fibrin CONDITIONS: T = 37°C, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM 5,5-Diethylbarbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium Acetate...

Appl report 341 (15 KB )

sigma-aldrich.com/supelco Application Report 341 LC-MS Analysis of Barbituates Using Ascentis ™ C8 Seven barbituates are separated on Ascentis C8 and detected by mass spectrometry. Key Words LC-MS, Ascentis C8, drugs of abuse, 34966, 39253, 57-44-3, B-0375, 50-06-6, P-1636, 76-73-3, S-1503, 57-33-0,...

T9000 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T9677 - Enzyme Assay (24 KB)

T9677 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

T5772 - Enzyme Assay (50 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T8397 - Enzyme Assay (51 KB)

SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37ΕC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate Solution (Soln A) (Prepare 275 ml in deionized water using Sodium ...

T6759 - Enzyme Assay (24 KB)

T6759 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

T4648 - Enzyme Assay (24 KB)

T4648 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

T6634 - Enzyme Assay (24 KB)

T6634 Page 1 of 4 BEPLAS01 Revised: 09/04/95 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of THROMBIN (EC 3.4.21.5) PRINCIPLE: Thrombin Fibrinogen > Fibrin CONDITIONS: T = 37EC, pH 7.35 METHOD: Fibrometer REAGENTS: A. 143 mM Diethyl Barbiturate and 143 mM Sodium Acetate So...

P5543 - Datasheet (13 KB)

ANTI-HUMAN Placental Lactogen (HPL) Developed in Goat Whole Antiserum Product No. P5543 Product Description The antiserum is developed in goat using human placental lactogen as the immunogen. The product is provided as an undiluted antiserum containing 15 mM sodium azide as a preservative.* RIA SYST...

Acylase I - Colorimetric (23 KB)

SSACET01 Page 1 of 5 Revised: 11/11/95 Enzymatic Assay of ACYLASE I (EC 3.5.1.14) PRINCIPLE: N-Acetyl-L-Methionine + H 2O Acylase I > L-Methionine + Acetic Acid CONDITIONS: T = 37°C, pH = 8.0, A570nm, Light path = 1 cm METHOD: Colorimetric REAGENTS: A. 100 mM N-Acetyl-L-Methionine Solution, pH 8.0 a...