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28 matches found for barbital buffer

B5934 - Datasheet (22 KB)

Barbital Buffer Product Number B 5934 Store at Room Temperature Product Description Barbital Buffer is a general purpose electrophoresis buffer for separation of serum proteins, lipoproteins, and isoenzymes such as lactate dehydrogenase and creatine kinase. The buffer provides excellent resolution o...

A5304 - Datasheet (21 KB)

AGAROSE High EEO Product Number A 5304 Store at Room Temperature Procedure Preparation and Use of Counter Immunoelectrophoresis Gels 1. Dissolve one vial Barbital Buffer (Product No. B 6632) in one liter deionized water. 2. Prepare 30 ml of 1% agarose solution (sufficient for two 85 x 100 mm gels) b...

Nuclease P1 (17 KB)

Revised: 09/08/94 Page 1 of 3 Enzymatic Assay of NUCLEASE P1 (EC 3.1.30.1) RNA as Substrate PRINCIPLE: Nuclease P1 RNA + H2O + > Acid Soluble Polynucleotides Abbreviation used: RNA = Ribonucleic Acid CONDITIONS: T = 37°C, pH = 5.3, A260nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Deter...

P5543 - Datasheet (13 KB)

ANTI-HUMAN Placental Lactogen (HPL) Developed in Goat Whole Antiserum Product No. P5543 Product Description The antiserum is developed in goat using human placental lactogen as the immunogen. The product is provided as an undiluted antiserum containing 15 mM sodium azide as a preservative.* RIA SYST...

Acylase I - Colorimetric (23 KB)

SSACET01 Page 1 of 5 Revised: 11/11/95 Enzymatic Assay of ACYLASE I (EC 3.5.1.14) PRINCIPLE: N-Acetyl-L-Methionine + H 2O Acylase I > L-Methionine + Acetic Acid CONDITIONS: T = 37°C, pH = 8.0, A570nm, Light path = 1 cm METHOD: Colorimetric REAGENTS: A. 100 mM N-Acetyl-L-Methionine Solution, pH 8.0 a...

A2156 - Enzyme Assay (22 KB)

SSACET01 Page 1 of 5 Revised: 11/11/95 SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of ACYLASE I (EC 3.5.1.14) Sigma Prod. No. A-2156 PRINCIPLE: N-Acetyl-L-Methionine + H 2O Acylase I > L-Methionine + Acetic Acid CONDITIONS: T = 37°C, pH = 8.0, A570nm, Light path = 1 cm METHOD: Colorimetric ...

ISO2 - Bulletin (90KB)

Mouse Monoclonal Antibody Isotyping Reagents Catalog Number ISO2 Storage Temperature 2–8 °C TECHNICAL BULLETIN Product Description The Mouse Monoclonal Antibody Isotyping Reagents are intended for use in the qualitative isotype determination of mouse monoclonal antibodies. Antibodies in hybridoma ce...

biofiles5_complement (1 MB )

Complement Proteins and Reagents The complement system is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors. This cascade ultimately results in induction of the inflammatory response, phagocyte chemotaxis and opsonization, and cell lysis. Compl...

Cell Culture Manual (33 MB)

75558_CCM_Cover_r5_CORR.indd 1 4/25/2011 1:08:07 PM Biointegrity. Cultivating healthy cell growth means depending on a quality mix of media, sera and reagents. That’s why we offer an unparalleled line of cell culture solutions. From classical and specialty media, to fetal bovine serum, supplements a...

(2 MB)

Look closer at your MILLIPLEX ® multiplex biomarker assays Tips and Tricks The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2 Why just multiplex when you can use MILLIPLEX ®? Note: Alternate methods presented in this guide may deviate from the protocol. These met...

MILLIPLEX Tips and Tricks (2 MB)

Look closer at your MILLIPLEX ® multiplex biomarker assays Tips and Tricks The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2 Why just multiplex when you can use MILLIPLEX ®? Note: Alternate methods presented in this guide may deviate from the protocol. These met...

Tips and Tricks: The Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX MAP (9 MB)

Tips and Tricks The Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX ® map The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. 2 Why just multiplex when you can use MILLIPLEX ®? Note: Alternate methods presented in this gui...

Tips and Tricks: The Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX MAP (9 MB)

Tips and Tricks The Power of Multiplex Biomarker Analysis from the makers of MILLIPLEX ® map The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2 Why just multiplex when you can use MILLIPLEX ®? Note: Alternate methods presented in this guide may deviate from the p...

MILLIPLEX Tips and Tricks (2 MB)

Look closer at your MILLIPLEX ® multiplex biomarker assays Tips and Tricks The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. 2 Why just multiplex when you can use MILLIPLEX ®? Note: Alternate methods presented in this guide may deviate fro...

M7151 - Datasheet (23 KB)

α α α α 2-MACROGLOBULIN From Human Plasma Product No. M 7151 Store below 0 °C Product Description α 2-Macroglobulin is prepared from human plasma by a modification of the method of Swenson and Howard. 1 All plasma used has been tested for and found to be negative for HB sAg antigen and HIV antibody....

T2652 - Datasheet (72KB)

Anti-L-Thyroxine (T4) produced in rabbit, whole antiserum Catalog Number T2652 Product Description Anti-L-Thyroxine (T4) is produced in rabbit using as immunogen L-Thyroxine-BSA (T4-BSA). Reagent Supplied as whole antiserum containing 15 mM sodium azide as a preservative. Precautions This product is...

T3901-011K4817 - Datasheet (34KB)

Mouse Monoclonal Anti-L-Thyroxine (T4) Immunoglobulin Fraction of Cell Culture Supernatant Clone 16A Product Number T 3901 Lot Number 011K4817 Product Description Monoclonal anti-Thyroxine (T4) is derived from the hybridoma (16A) produced by the fusion of mouse myeloma cells and BALB/c mice splenocy...

T3776 - Datasheet (54KB)

MOUSE MONOCLONAL ANTI-TRIIODOTHYRONINE (T3) Immunoglobulin Fraction of Cell Culture Supernatant Clone T3 -10 Product Number T3776 Product Description Monoclonal Anti-Triiodothyronine (T 3) is derived from the hybridoma (T3 -10) produced by the fusion of mouse myeloma cells and BALB /c mice splenocyt...

T2652. - Product Information Sheet (0 KB)

Anti-L-Thyroxine (T4) produced in rabbit, whole antiserum Catalog Number T2652 Product Description Anti-L-Thyroxine (T4) is produced in rabbit using as immunogen L-Thyroxine-BSA (T4-BSA). Reagent Supplied as whole antiserum containing 15 mM sodium azide as a preservative. Precautions This product is...

Chiral Method Development Techniques for Analytical Preparative HPLC (1 MB )

Chiral Method Development Techniques for Analytical Preparative HPLC Method Development Techniques � Column coupling enables simultaneous multi-column screening CHIROBIOTIC R/V/T Method 1 - Fast � Generic screening methods for fast chiral method development with switching valves for: A. 9 columns, 9...

T2777 - Datasheet (54KB)

ANTI-TRIIODOTHYRONINE (T3) Developed in Rabbit Whole Antiserum Product No. T2777 Product Description The antiserum is developed in rabbit using L-triiodo-thyronine-BSA (T 3-BSA) as the immunogen. Reagents The product is provided as an undiluted antiserum containing 0.1% sodium azide as a preservativ...

T3901-071K4842 - Datasheet (34KB)

Mouse Monoclonal Anti-L-Thyroxine (T4) Clone 16A Immunoglobulin Fraction of Cell Culture Supernatant Product Number T 3901 Lot Number 071K4842 Product Description Monoclonal Anti-Thyroxine (T 4) is derived from the hybridoma (16A) produced by the fusion of mouse myeloma cells and BALB/c mice splenoc...

H1511 - Datasheet (46 KB)

HAPTOGLOBIN From Pooled Human Plasma Product No. H1511 Store at 2-8°C Product Description Haptoglobin is found in normal plasma at a concentration of 30-190 mg/dl and accounts for 0.4-2.6% of the total plasma proteins. Some conditions such as cancer and coronary artery disease can raise this level, ...

MM-Universal Reagents for Microscopy-Brochure (9 MB)

Universal Reagents for Microscopy The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2019-20188-Universal-Reagents-for-Microscopy-Brochure_MRK.indd 1 4/26/19 1:59 PM 2 Universal Reagents for Microscopy For over 100years, dyes and solutions of dyes have been part of...

Supelco Reporter, Volume 31.2 (3 MB)

Applications Newsletter Volume 31.2 pg. 12 INCREASE REMOVAL OF FAT AND PIGMENT FROM AVOCADO EXTRACTS pg. 18 LC-MS ANALYSIS OF WARFARIN IN PLASMA SAMPLES Introducing UHPLC Columns Setting a New Performance Standard pg. 3 sigma-aldrich.com/analytical Reporter 31.2 | 2 Table of Contents Pharmaceutical ...

Cerilliant catalogue (8 MB)

The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. Accurate reference, accurate results Cerilliant ® Certified Reference Materials for your specific applications Contents Multi-Component Drug 9 Standards and Kits Acylcarnitines 12 Alcohol S...

Titan UHPLC Columns: Setting a New Performance Standard (5 MB)

Applications Newsletter Volume 55 October 2013 pg. 3 pg. 12 INCREASE REMOVAL OF FAT AND PIGMENT FROM AVOCADO EXTRACTS pg. 18 LC-MS ANALYSIS OF WARFARIN IN PLASMA SAMPLES Introducing UHPLC Columns Setting a New Performance Standard sigma-aldrich.com/analytical Reporter 55 | 2 Pharmaceutical Table of ...

Discovery HPLC Columns for Small Molecule Separations (1 MB )

1 sigma-aldrich.com/supelco The Discovery Suite of Reversed-Phase HPLC Columns Gives Better Separations in Less Time. Rediscover Method Development HPLC Columns for Pharmaceutical Analysis and Purification ® SUPELCO DISCOVERY HPLC COLUMNS 2 Table of Contents Discovery Column Quick Look-Up Guide .......