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以酪蛋白为底物的蛋白酶酶分析

为了标准化在 Sigma-Aldrich St.Louis 使用酪蛋白作为底物的蛋白酶的酶测定程序。 P4630, P4755, P0384, P5380, P7431, P6141, P1512, P9040, P5147, P5647, P8775, P7026, P4032, P8038, P8298, P2789, P4789, P6670, P3910, P4806,以及所有指定的杂质。 3.1 单位定义——一个单位在 37ºC pH 7.5 时每分钟水解酪蛋白产生相当于 1.0 μmole 酪氨酸的颜色。颜色与 Folin 和 Ciocalteau 试剂一致。 3.2 纯化水——由...

Enzymatic Assay of Protease Using Casein As a Substrate

• • Download PDF • To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate at Sigma-Aldrich St. Louis. P4630, P4755, P0384, P5380, P7431, P6141, P1512, P9040, P5147, P5647, P8775, P7026, P4032, P8038, P8298, P2789, P4789, P6670, P3910, P4806, and any specified impu...

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products. The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction: Unit Definition = One unit of ficin will produce a ΔA280 of 1.0 per minute at pH 7.0 at 37 °C when measuring TCA soluble products from casein...

General Protease Substrates - Proteolytic Enzymes and Substrates

Sigma-Aldrich Online Catalog Product List: General Protease Substrates

Casein and Other Milk Proteins - Proteins and Derivatives

Sigma-Aldrich Online Catalog Product List: Casein and Other Milk Proteins

通用型蛋白酶活性检测:酪蛋白底物法

操作流程 A. 底物 蛋白酶可切割肽键。在实验室中,通常需要测量或比较蛋白酶的活性。Sigma的非特异性蛋白酶活性检测可作为一种标准的方法测定蛋白酶活性,这也是我们在进行质控时所使用的方法。该检测以酪蛋白作为底物,当用待测蛋白酶消化酪蛋白时,酪氨酸与其他氨基酸和肽段一起释放。Folin & Ciocalteus苯酚或Folin试剂可主要与游离的酪氨酸发生反应并产生蓝色的发色基团,并可利用分光光度计的吸光值进行定量和测量。酪蛋白释放的酪氨酸越多,则产生的发色基团也越多,表明蛋白酶的活性越强。检测时,将由蛋白酶活性产生的吸光值与标准曲线比较,而标准曲线则是通过将已知量的酪氨酸与F-C试剂反应产生,...

Proteolytic Enzymes Substrates Procedures

Substrate Product # Substrate Procedure C0528 Casein fluorescein isothiocyanate from bovine milk, Type III Fluorometric Protease Assay (PDF 75.3 KB) C3777 Casein fluorescein isothiocyanate from bovine milk, Type II Fluorometric Protease Assay (PDF 75.3 KB) C7078 Casein bovine milk, Technical Grade C...

Universal Protease Activity Assay: Casein as a Substrate

A. Abstract Proteases break peptide bonds. It is often necessary to measure and/or compare the activity of proteases. This non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases for quality control purposes. In this assay, casein acts as a...

Universal Protease Activity Assay: Casein as a Substrate [VIDEO]

A. Abstract Proteases break peptide bonds. It is often necessary to measure and/or compare the activity of proteases. Our non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality control procedures. In...

タンパク質&酵素

• p~6 • A • B~C • D~G • H~L • M~O • P~S • T~Z • α~γ p~6 製品番号 製品名 CAS番号 純度 溶解度 形状 保管温度 分子量 P6001 p-Phenylenediamine 106-50-3 ≥98% (HPLC) 50mg/mL, Ethanol powder or flakes or chunk(s) 108.14 L9756 (−)-Tetramisole hydrochloride 16595-80-5 ≥99% (GC) 50 mg/mL, H2O powder 240.75 M107 (+)-MK-801 hydrogen m...