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20 matches found for cannabis

GC Analysis of Terpenes in Cannabis on Equity®-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

sample preparation 30 min equilibration at 40 °C prior to extraction sample preparation fiber postbake after extraction, 3 min at 270 °C sample/matrix 0.5 g dried cannabis in 10 mL headspace vial desorption process 3 min, 270 °C extraction 20 min, headspace, 40 °C SPME fiber Divinylbenzene/Carboxen/...

LC/MS/MS Analysis of Pesticides in Cannabis on the Ascentis RP-Amide after QuEChERS Extraction and Cleanup Using Supel QuE Verde

column Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3.0 µm guard column Ascentis RP-Amide Supelguard guard cartridge, 2 cm x 2.1 mm I.D., 5 µm mobile phase [A] 5 mM ammonium formate, 0.1% formic acid in 95:5 water:acetonitrile; [B] 5 mM ammonium formate, 0.1% formic acid in 5:95 water:acetonitrile gradie...

LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis® Express 2.7µm Phenyl-Hexyl after Cleanup using Supel™ Tox AflaZea SPE

sample preparation Solid Phase Extraction sample/matrix cannabis extract spiked with aflatoxins at corresponding concentrations of 6 ppb each (for Aflatoxin B2 and G2) and 24 ppb each (for Afltoxin B1 and G1) in cannabis SPE tube/cartridge Supel Tox AflaZea SPE, 6 mL (55314-U) sample addition 2 mL o...

GC Analysis of Pesticide Residues in Cannabis on SLB®-5ms after QuEChERS Extraction then QuEChERS Cleanup using Supel™ QuE Verde

sample preparation Solid Phase Extraction (QuEChERS, Dispersive) sample preparation place supernatant in amber low adsorption vial for GC/MS/MS analysis. sample/matrix ground cannabis, 1 gm extraction tube Supel QuE Citrate (55227-U) extraction process Add 10 mL water to sample, allow to sit for 30 ...

UHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis® Express RP-Amide

sample preparation A 100 mg sample of dried cannabis sativa leaves was extracted with 50 mL of ethanol:water (50:50, v/v) for 15 mins in an ultrasonic bath. (The sample was then filtered through a 0.45 µm Millex LH filter prior to LC/MS analysis.) column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D...

HPLC Analysis of Spice Cannabinoids JWH-018, JWH-073 and Metabolites on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U) column temp. 35 °C mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in water:acetonitrile (10:90) gradient 20% B held for 3 min; 20 to 100% B in 0.1 min; 100% B held for 2 min flow rate 0.5 mL/min pressure 2...

HPLC Analysis of Cannabinoids using Ascentis® Express RP-Amide

column Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (50774-U) column temp. 35 °C mobile phase 5 mM ammonium acetate (pH 4.5 with acetic acid) in 20:80, acetonitrile:water flow rate 1.0 mL/min pressure 1450 psi (100 bar) sample each compound 50 µg/mL in water:methanol (50:50) inject...

UHPLC Analysis of Cannabinoids on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U) column temp. 35 °C mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile gradient 50% B to 100% B in 10 min flow rate 0.4 mL/min pressure 6300 psi (434 bar) sample 50 ng/mL each in methanol injection 1 µL ...

HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U) column temp. 30 °C mobile phase [A] 5 mM ammonium formate water; [B] 5 mM ammonium formate (95:5, acetonitrile:water) gradient 68 to 100% B in 8 min; held at 100% B for 2 min flow rate 0.6 mL/min pressure 4351 psi (300 bar)...

UHPLC Analysis of Cannabinoids on Ascentis Express C18

instrument Shimadzu Nexera X2 column Ascentis Express C18, 15 cm x 3.0 mm I.D., 2.7 µm mobile phase [A] water (0.1% formic acid); [B] acetonitrile (0.085% formic acid) gradient 70% B to 88% B in 6 min flow rate 1.0 mL/min pressure 5075 psi column temp. 30 °C detector UV, 220 nm injection 0.6 µL samp...

UHPLC/MS Analysis of Ten Cannabinoids on Ascentis® Express RP-Amide

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 µm particles (53913-U) column temp. 35 °C mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile gradient 60% B to 100% B in 10 min flow rate 0.4 mL/min pressure 7600 psi (524 bar) sample 1000 ng/mL each in methanol injectio...

HPLC Analysis of Spice Cannabinoids on Ascentis® Express F5

column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U) column temp. 30 °C mobile phase [A] 50 mM ammonium formate; [B] water; [C] acetonitrile; (10:35:55, A:B:C) flow rate 0.6 mL/min pressure 4075 psi (281 bar) sample 100 µg/mL in 45:55 water:acetonitrile injection 3 µL detector...

UHPLC/MS Analysis of Cannabinoids on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 µm particles (50813-U) column temp. 35 °C mobile phase [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile gradient 60% B to 100% B in 10 min flow rate 0.4 mL/min pressure 7200 psi (496 bar) sample 100 ng/mL each in methanol injection 1 µL...

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Chromolith® HR RP-18e

instrument Dionex/Thermo Electron Ultimate 3000 HPLC column Chromolith HR RP-18e, 10 cm x 4.6 mm (1.52022) mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile isocratic 30/70 [A]/[B] flow rate 1.5 mL/min pressure 1100 psi (75,8 bar) column temp. 30°C detector UV at 230nm...

LC/MS/MS Analysis of Spice Cannabinoids in Plasma on Ascentis® Express F5 after Solid Phase Extraction (SPE) using HybridSPE®-Phospholipid

sample/matrix rabbit plasma, unfiltered K2-EDTA spiked with Spice cannabinoids (5 ng/mL each) SPE well plate HybridSPE-Phospholipid, 96-well plate, 50 mg bed wt., 2 mL well vol (575656-U) sample addition to each well add 100 µL plasma, followed by a 300 µL of 1% formic acid in acetonitrile, agitate ...

UHPLC (Isocratic) Analysis of 14 Cannabinoids on Ascentis® Express C18

instrument Dionex/Thermo Electron Ultimate 3000 HPLC column Ascentis Express C18, 15 cm x 2.1 mm, 2.7 µm mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile isocratic 30/70 [A] / [B] flow rate 0.6 mL/min pressure 6390 psi column temp. 20 °C detector UV at 230nm injection...

UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18

column Titan C18, 10 cm x 2.1 mm, 1.9 µm particles (577124-U) column temp. 35 °C mobile phase [A] 0.1% formic acid in 95:5, water:acetonitrile; [B] 0.1% formic acid in 5:95, water:acetonitrile; gradient 25% B held for 1 min; to 45% B in 0.5 min; 45% B held for 4.5 min; to 80% B in 0.5 min; 80% B hel...

GC Analysis of Residual Solvents in Hemp Extract on Supel-Q™ PLOT after Headspace SPME using 75 μm Carboxen®/PDMS Fiber

sample preparation fiber post bake: 2 min, 320 °C sample preparation Solid Phase Microextraction sample/matrix 5 g sample in 10 mL vial desorption process 3 min, 320 °C; split 10:1 extraction 5 min, headspace, 40 °C SPME fiber SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS) (57319) colu...

GC Analysis of Tetrahydrocannabinol (THC) and Carboxytetrahydrocannabinol (THCCOOH) in Surface Water on SLB®-5ms after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber, On-Fiber Derivatization

sample preparation post-extraction derivatization: 500 µL MSTFA in 10 mL vial, headspace, 30 min, 60 °C sample preparation fiber post-bake: 10 min, 270 °C sample preparation SPME sample/matrix 8 mL surface water in 10 mL vial, adjusted to pH 2 with HCl, spiked with THC and THCCOOH at 50 ng/L each co...

Selecting a GC Column for Specific Industries/Applications

We know that only the most accurate analytical products will do. That’s why we offer the Supelco portfolio of analytical GC columns, providing you the accuracy, precision and consistency you need. Gas chromatography, first established in the 1950′s, is a mature analytical technique with many establi...