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42 matches found for chondrocytes

Chondrocytes - Primary Cells

Normal and diseased primary chondrocytes from human and animal tissue are low passage, high purity, and performance tested. Four types of normal and diseased human chondrocytes are available and can be useful cellular models for studying differences between normal and pathological conditions of rheu...

Human Chondrocytes Osteoarthritis (HC-OA) Culture Protocol

Storage Preparation for Culturing Culturing HC-OA Subculturing HC-OA Materials A. Cryopreserved Vials (402OA-05a) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storag...

Human Chondrocytes (HC) Culture Protocol

Storage Preparation for Culturing Culturing HC Subculturing HC Materials A. Cryopreserved Vials (402-05a, 402-05f) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen stora...

Human Chondrocytes Rheumatoid Arthritis (HC-RA) Culture Protocol

Storage Preparation for Culturing Culturing HC-RA Subculturing HC-RA Materials A. Cryopreserved Vials (402RA-05a) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storag...

Primary Human Chondrocytes and Chondrocyte Growth Media

Related Links • Primary Cell Culture • Technical Article: Primary Cell Culture Overview • Primary Cell Culture FAQs • Mammalian Cell Lines • Stem Cell Culture • 3D Cell Culture • Cell Culture Media • Cell Culture Supplements • Cell Dissociation Reagents • Fetal Bovine Serum • Stericup® Media Filtrat...

Mesenchymal Stem Cell Markers and Antibodies – Sigma-Aldrich

• Mesenchymal Stem Cell Markers • Fibroblasts and Connective Tissue Cells • Chondrocytes and Cartilage Cells • Adipocytes and White Fat Tissue Cells • Osteoblasts and Bone Cells • Smooth, Cardiac, and Skeletal Muscle Cells • Available Mesenchymal Stem Cell Markers and Antibodies In general, stem cel...

Human Mesenchymal Stem Cells and Growth/Differentiation Media

Related Links • ISCT guidelines on multipotent human MSC characterization • Differentiation of Human MSCs into Osteocytes, Adipocytes and Chondrocytes • Neural Differentiation of Human MSCs • Fibronectin Coating Protocol • Stem Cell Culture • Primary Cell Culture • 3D Cell Culture • Mammalian Cell L...

Interleukin-17 - Interleukins (IL)

Structure: Cysteine knot, Homodimer/heterodimerSecreted by: T cells, neutrophils, NK and NKT cells, chondrocytesFunctions: Induce proinflammatory cytokines and chemokines, chondrogenesis, osteogenesis, induction of metalloproteinasesDisease areas: Rhematoid arthritis, allergic and inflammatory disea...

Interleukin-8 - Interleukins (IL)

Structure: MonomerSecreted by: Epithelial cells, monocytes, macrophages, neutrophils, lymphocytes, chondrocytes, keratinocytesFunctions: Activation of neutrophils, NK cells, T cells and eosinophils, angiogenesis, mobility of hematopoietic stem cellsDisease areas: Infections, psoriasis, Rheumatoid Ar...

FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms CDEP, PLEKHC2, PPP1R75
Species Human (10160) , Species Mouse (223254) , Species Rat (306183) , Species chicken (418781) , Species domestic cat (101098028) , Species cow (531927) , Species Horse (100060841) , Species dog (476970) , Species sheep (101110152) , Species Domestic Rabbit (100352920) , Species naked mole-rat (101717806) , Species domestic guinea pig (100714154)
Summary: This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Mesenchymal Stem Cell Reagents - General Stem Cell Biology

Mesenchymal stem cells (MSC) have the capacity to form different cell types, such as adipocytes, chondrocytes, osteocytes, hepatocytes, cardiomyocytes and neurons, given exposure to different inductive agents. The products included here are commonly used in MSC research, including numerous growth fa...

Neuronal Mesenchymal Stem Cells

Human mesenchymal stem cells (MSCs) represent a promising tool in regenerative medicine because of their ability to self-renew and differentiate into a variety of specialized cells including adipocytes, osteocytes, chondrocytes and myocytes. These multipotent stem cells can be easily isolated from v...

Mesenchymal Stem Cell Culture

• Mesenchymal Stem Cell Lines • Mesenchymal Stem Cell Media • Mesenchymal Stem Cell Markers • Stem Cell Culture Protocols Human mesenchymal stem cells (MSCs) are multipotent adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotype...

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

Introduction Methods Human MSC Expansion in Human Platelet Lysate Human MSC Differentiation FACS Mesenchymal Stem Cell Marker Analysis Results Conclusions Materials Mesenchymal stem cells (MSCs) have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes s...

Mesenchymal Stem Cell Culture Protocols | MSC Culture Media

Introduction Methods Isolation of Mesenchymal Stem Cells Expansion of Human Mesenchymal Stem Cells Preparation of Coated Plates Thawing of Mesenchymal Stem Cells Expansion of Mesenchymal Stem Cells Differentiation of Human Mesenchymal Stem Cells Osteogenesis of Mesenchymal Stem Cells Adipogenesis of...

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Related Products • Mesenchymal Stem Cell Culture • Stem Cell Culture • Stem Cell Protocols • Mobius Single Use Bioreactors • 3D Cell Culture • Primary Cell Culture • ECACC Cancer Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Protein • Antibodies • B...

Complex Carbohydrates Analysis: Enzymes, Kits, and Reagents

BioFiles 2007, 2.3, 1. Complex carbohydrates compose the most abundant class of biopolymers on earth. Because of their structural and functional diversity, they have found applications in biomedical, nutritional, textile, cosmetic and countless other industries. The complex carbohydrates of the extr...

Fibronectin (FN)

• • Download PDF • Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein composed of two nearly identical disulfide bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma, therefore poly...

Mesenchymal Stem Cell Differentiation

Mesenchymal stem cells (MSCs) are fibroblastoid multipotent adult stem cells with a high capacity for selfrenewal and differentiation. These cells have been isolated from several human tissues, including bone marrow, adipose tissue, umbilical cord matrix, tendon, lung, and the periosteum.1 Recently ...

Mesenchymal Stem Cell Characterization

Mesenchymal stem cells (MSCs) are fibroblast-like multipotent adult stem cells derived from multiple tissue (including bone marrow, adipose tissue, umbilical cord, and dental pulp) with the capacity to self-renew. Historically, researchers have isolated these stem cells using various methods and a s...

Modulated Gene Expression in 3D Cell Culture

In-vitro cell models are essential for validating the efficacy of drug candidates. However, large numbers of compounds are discontinued in subsequent phases of clinical studies. The reason for these failures is poor predictability of 2D cell cultures1, which could not mimic physiological conditions....

HyStem™ Glossary of Terms

A | B | C | D | E | F | G | H | I | L | M | N | O | P | R | S | T | U A Acinus (plural: acini) - The word comes from the latin word “grape”. It refers to the sac-like termination of a secretary exocrine gland. In vitro, mammary epithelial cells cultured in a laminin-rich matrix form acinus-like sphe...

Primary Cell Culture Basics | Introduction Of Cell Culture

Related Links • Primary Cell Culture Homepage • Introduction to Primary Cell Culture Video • Primary Cell Culture FAQs • Stem Cell Culture • 3D Cell Culture • Mammalian Cell Lines • Cell Culture Media • Cellular Assays • ECACC Cell Culture Handbook Primary cells most closely represent the tissue of ...

Pluripotent and Multipotent Stem Cells

Related Products • Stem Cell Culture • Stem Cell Protocols • Pluripotent Stem Cell Culture • Neural Stem Cell Culture • Mesenchymal Stem Cell Culture • Hematopoietic Stem Cell Culture • Cell Culture Media and Reagents • Growth Factors • ECM Protein • Stem Cell Antibodies • CRISPR Genome Editing • 3D...

What is Hyaluronan?

Related Links • 3D cell culture • 3D hydrogels • HyStem® hydrogels Related Products • Cell culture • 3D cell culture • Brief Summary • Nomenclature • Chemistry and Physical Properties • Biological Roles • Degradation of Hyaluronan • Hyaluronan Synthesis • Glycosil vs. Unmodified HA • History of Hyal...

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the cell culture vessel, have proven to be efficient methods to better mimic in vivo cell behavior. We de...

Vascular Pericytes

Figure 1. Vascular pericyte overview. Pericytes surround blood vessel capillaries and endothelial cells, neurons and astrocytes within the blood-brain-barrier (BBB) neurovascular unit. Pericytes, also known as mural cells, are multipotent perivascular cells that play an important role in the develop...

Avocado (Persea americana) | Plant Profiler

Synonyms / Common Names / Related Terms Abokado, aguacate, ahuacate, ahuacatl, alligator pear, avocado pear, Avocato, Persea americana, Persea americana var. drymifolia Blake, Persea gratissima, Persea leiogyna, Persea nubigena var. guatamalensis L., Persea persea, Laurus persea. Mechanism of Action...

Collagen

Collagen is the most abundant mammalian protein and the main component of connective tissues. Typically collagen is first synthesized as individual glycine-rich preprocollagen monomeric strands from the ribosomes in the rough endoplasmic reticulum (RER). After transport to the lumen of the RER signa...

FGFR

Fibroblast growth factors (FGFs) comprise a large family of signaling polypeptides. In the adult organism, FGFs are homeostatic factors and function in tissue repair and response to injury. In the embryo, FGFs regulate cell proliferation, migration, differentiation and survival. When inappropriately...

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a variety of cells (as indicated in the product manual). Scientific publications continue to add to the...