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138 matches found for fbs

FBS Select - Fetal Bovine Serum (FBS) for cell culture

FBS SelectProducts designated FBS Select meet standards for specialized cell and tissue culture including supplementation of stem cells, cardiomyocytes, and cells cultured for immunotherapeutics. FBS Select products include sera that are:EmbryoMax ES (embryonic stem) cell-qualifiedMesenchymal stem c...

FBS Classic - Fetal Bovine Serum (FBS) for cell culture

FBS ClassicFBS suitable for general cell culture applications and includes the following to ensure quality and consistency from lot-to-lot:Must pass rigorous testing of up to 48 defined quality release parametersTriple filtered with 0.1 m membraneUSA, USDA, Canada, Australia, and other origin

FBS Premier - Fetal Bovine Serum (FBS) for cell culture

FBS PremierFBS suitable for biomanufacturing and other applications where enhanced testing, documentation, traceability and certifications may be required. Irradiated FBS products are further complemented by our SER-TAIN enhanced process validation.Stringent testing and release procedures based on i...

Protocol for Charcoal-stripping FBS to Deplete Hormones

Related Products • Cell culture sera • Fetal Bovine Sera • Serum Replacements • Cell Culture Media • Supplements & reagents • Mammalian Cell Lines • Primary Cells • Hormones Related Links • Request a Serum Sample • Serum University • Serum Learning Center • Serum-free Media • Cell Culture Troublesho...

Fetal Bovine Serum (FBS) for cell culture - Fetal Bovine Serum (FBS) and Other Animal Sera

Fetal bovine serum (FBS) is the most widely used growth supplement for cell culture media because of its high content of embryonic growth promoting factors. When used at appropriate concentrations it supplies many defined and undefined components that have been shown to satisfy specific metabolic re...

Fetal Bovine Serum (FBS) and Other Animal Sera - Cell Culture

Fetal bovine serum (FBS) contains embryonic growth factors that make it an excellent choice as a growth supplement in cell culture. Growth factors, hormones, proteins, vitamins, and trace elements in FBS have been shown to contribute to the metabolic requirements of cells. Many of these biomolecules...

Freezing and Thawing FBS and Other Sera

Related Products • Cell culture sera • Fetal Bovine Sera • Serum Replacements • Cell Culture Media • Supplements & reagents • Mammalian Cell Lines • Primary Cells • Stem Cells • Freezing Container Related Links • Request a Serum Sample • Serum University • Serum Learning Center • Serum-free Media • ...

Frequently-asked questions about gamma-irradiated FBS (serum) and other products for cell culture

Related Products • Cell Culture Sera • Fetal Bovine Sera • Serum Replacements • Cell Culture Media • Supplements & Reagents • Mammalian Cell Lines • Primary Cells • Stem Cells Related Links • Request a serum sample • Serum learning center • Serum University • Serum-free media • Cell culture troubles...

Newborn, Calf and Adult Bovine Sera - Fetal Bovine Serum (FBS) and Other Animal Sera

Sigma-Aldrich Online Catalog Product List: Newborn, Calf and Adult Bovine Sera

F-box protein 8
Synonyms FBS, FBX8
Species Human (26269) , Species Mouse (50753) , Species Rat (306436) , Species sheep (101119879) , Species Domestic Rabbit (100358150) , Species naked mole-rat (101713357) , Species domestic guinea pig (100726283) , Species Zebrafish (678549) , Species cow (538528) , Species Horse (100061095) , Species domestic cat (101088075) , Species dog (477359) , Species chicken (422565)
Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq, Jul 2008]

Bovine Spongiform Encephalopathy (BSE)-free fetal bovine serum (FBS)

Related Products • Cell culture sera • Fetal Bovine Sera • Cell Culture Media • Supplements & reagents • Mammalian Cell Lines • Primary Cells • Stem Cells Related Links • Request a Serum Sample • Serum University • Serum Learning Center • Cell Culture Troubleshooting • Cell Freezing Although the age...

Microbe selection for the SER-TAIN™ gamma irradiation FBS validation process

Related Products • Cell Culture Sera • Fetal Bovine Sera • Cell Culture Media • Supplements & Reagents • Mammalian Cell Lines • Primary Cells • Stem Cells • Mycoplasma Detection • Cell Culture Antibiotics Related Links • Request a serum sample • Serum learning center • Serum University • Serum-free ...

Pooled Human Serum for Cell Culture - Fetal Bovine Serum (FBS) and Other Animal Sera

Before pooling, each individual donor unit is tested and found negative for Hepatitis B [HBsAg], Hepatitis C [Anti-HCV], and Human Immunodeficiency Virus [HIV] antibodies by ELISA. Final product is tested and found negative for HBsAg, Hepatitis A, and HIV. Biological performance is assessed using ce...

Horse Sera and Sera from Other Species - Fetal Bovine Serum (FBS) and Other Animal Sera

Browse Sigma-Aldrich's Horse Sera and Sera from Other Species to find products in Horse Sera, Sera from Other Species

Serum-Free Stem Cell Media & Supplements

Historically, fetal bovine serum (FBS) has been the supplement of choice for in vitro mammalian cell culture including stem cells. However, due to the undefined composition of FBS, researchers have transitioned to more chemically defined serum-free and xeno-free media and supplements for stem cell r...

Serum for Cell and Tissue Culture: Frequently Asked Questions (FAQs)

Related Products • ECACC cell lines • Cell culture freezing media • Classical media • Bovine serum albumin • Human sera Related Information • Cell culture • Primary cell culture • Assays and Reagents • Stem cell culture • Growth factors and cytokines • 3D cell culture Request Sample Why do cell and ...

Cell Culture

• Stericup® & Steritop Bottle-top Filters • Sera / FBS • Liquid Media • Sterile Millex® Syringe Filters • CellASIC® ONIX2 System • Scepter™ 3.0 Automated Cell Counter Cell culture is fundamental to life science research in universities, biotechnology, pharmaceuticals, and industry. Whether your goal...

Mouse Embryonic Stem Cell Culture

• Transgenic Mouse Models • ESGRO Leukemia Inhibitory Factor • ES Cell Qualified FBS • Serum-Free/Feeder-Free Media • MEF Feeder Cells • Mouse Genotyping • Stem Cell Culture Protocols Transgenic and gene knockout technologies are powerful tools for studying gene function. A commonly used method for ...

MegaCell Media - Reduce Serum to 3%

Discover and save with our MegaCell™ line of enriched cell culture media products designed to reduce fetal bovine serum (FBS) supplementation to 3% or less. We have optimized this classic media through fortification of a multitude of specially engineered supplements, growth factors and amino acids. ...

Cell Dissociation with Trypsin | Mechanism in cell culture

Related Content • Trypsin • Trypsin Inhibitors • Antibiotics • Antibiotic selector tool • StableCell™ Trypsin Related Products • Cell Culture Media and Reagents • Growth Factors • ECM Protein • ECACC cell lines • Cell Freezing Media and Reagents • Fetal Bovine Serum (FBS) • Human Sera • Cell Dissoci...

MegaCell™ Media - Classical Media and Salts

The MegaCell media product line is a group of fortified media based on classical media formulations that have been enriched to support cell growth under reduced serum conditions. When supplemented with 3% FBS these media have been shown to support cell growth comparable to non-fortified classical me...

Serum Learning Center

With over 30 years in the cell culture and serum industry, we are pleased to share our knowledge and experience of FBS and other serum products. As a member of the International Serum Industry Association (ISIA), we share the same mission and vision of providing our customers quality serum products ...

Antibiotic Kill Curve

Related Products • Antibiotic selection guide • Antibiotic solutions • Antibiotic powders • Antibiotics • ECACC cell lines • Cell culture reagents • Growth factors • CRISPR Genome Editing • CRISPR Genome Editing • 3D Cell Culture • Cell dissociation • FBS Related Links • Optimization of Antibiotic S...

DMEM/Nutrient Mixture F-12 Ham (DMEM F12) - Classical Media and Salts

DMEM:F12 is a 50:50 mixture of DMEM and Ham’s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Hams Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary ...

Serum free cancer cell line media

Historically, cancer cell lines have been cultured almost exclusively in culture media supplemented with significant amounts of fetal bovine serum (FBS). Like other undefined media supplements, fetal bovine serum has unwanted physiological, genetic and epigenetic cellular effects1-4 and is known to ...

Techniques Glossary

Abbreviations ACDP Advisory Committee on Dangerous Pathogens BSA Bovine Serum albumin BSE Bovine spongiform encephalopathy BVDV Bovine viral diarrhoea virus FBS Fetal bovine serum EC European Community ECACC European Collection of Cell Cultures FDA Food and Drug Administration FCS Fetal Calf Serum H...

Common Cell Culture Problems: Cell Death

Related Links • Troubleshooting Guide Home • Poor Cell Growth • Precipitates • Poor Cell Attachment • Cell Clumping • Contamination • Cell Line Misidentification Related Products • Culture media • Fetal Bovine Serum (FBS) • Mycoplasma Detection • Culture Flasks • Trypsin • Antibiotics • Hoechst Stai...

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

• Healthy rapidly dividing mouse embryonic fibroblasts (MEF) cells • ExpressMag Super Magnetic Kit (SHM01) • 0.25% Trypsin EDTA solution (T3924) • Puromycin Solution (P9620) • DMEM (D5671) • MEF cell media – DMEM (D5671) supplemented with L-glutamine, 10% FBS (F0926) and nonessential amino acids (M7...

CHO Cells at Passage 20 stained for B-gal Expression

Figure 3. CHO Cells at Passage #20 stained for ß-gal Expression On day 1, CHO-K1 cells were seeded in a 6-well plate and were transfected with 2 µg/well of pBICEP-CMV-1+lacZ. Cells were grown in DMEM media supplemented with 10% FBS, 4 mM L-glutamine and 1 mg/ml G-418. At day 72, passage number reach...

B-gal Assay Normalized to Total Cell Protein

Figure 2. ß-gal Assay Normalized to Total Cell Protein CHO-K1 cells were seeded in a 6-well plate. Cells were transfected with 2 µg per well of DNA using Sigma's Escort II reagent and grown in DMEM supplemented with 10% FBS plus 4 mM L-glutamine. G-418 was introduced 48 hrs post transfection at 1 mg...

Cell Culture Serum - sera for cell culture

With more than 35 years of experience in the management and production of sera for use in cell culture, we offer many varieties of bovine serum (cow), chicken, caprine (goat), equine (horse), human, ovine (sheep), porcine (pig) and rabbit sera produced and tested for use in cell culture applications...