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110 matches found for hemocytometer

Counting cells using a hemocytometer - Cell Culture Protocol 6

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

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Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology. Various methods for monitoring cell proliferation have been developed. Counting cells using a hemocytometer is one of the simplest methods used to monitor c...

Scepter™ 3.0 Cell Counter - Videos

The Scepter™ 3.0 Cell Counter has new features that make getting precise cell counts easier than ever before. Watch these videos to see how the Scepter™ counter works, and to learn what’s new with version 3.0. Scepter™ 3.0 Product Features Video Your hemocytometer just can’t do this. Watch to learn ...

Validating the Guava ViaCount® Assay, an Automated Cell Counting/Viability Method for Use in ELISpot Assays

Many of the standard methods currently used for cell counts and viability assessments of cell suspensions, e.g., peripheral blood mononuclear cells (PBMC), do not provide very accurate or reliable measurements. The Guava PCA™ System with the ViaCount assay pro-vides enhanced sensitivity and better p...

Scepter™ 3.0 Cell Counter – How it Works

• Why automate your cell counting? • The gold standard for counting: Coulter impedance • Count and measure cell diameter with accuracy. Any cell, any buffer, every time. • New Features • Sample preparation For decades, hemocytometry was the gold standard for cell counting. A sample of media or buffe...

Subculture of Adherent Cell Lines: Cell Culture Protocol 3

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Culture Protocol 4: Subculture of Semi-Adherent Cell Lines

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Culture Protocol 2: Thawing of Frozen Cell Lines

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cryopreservation of Cell Lines : Cell Culture Protocol 7

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Fundamental Techniques in Cell Culture

Get the Guide Chapters Title 1 Introduction 2 Design and Equipment for the Cell Culture Laboratory 3 Safety Aspects of Cell Culture 4 Sourcing of Cell Lines 5 Cell Types & Culture Characteristics 6 The Cell Environment (including types of culture medium) 7 Cryopreservation and Storage of Cells 8 Goo...

Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter

Micron-sized beads are used in a variety of biological applications, ranging from daily validation of flow cytometer performance, to purification of fusion protein constructs from cell lysates. Depending on the nature of the assay, the beads may either possess magnetic properties or be labeled inter...

Cultrex® 3-D Culture Matrix™ Reduced Growth Factor Basement Membrane Extract, PathClear® Protocol

Product Number 3445-010-01 3D Culture is a cell culture method that provides cells with the necessary structure and signaling cues to direct reconstruction of the tissue architecture, and these methods provide physiologically predictive in vitro models for evaluating development and disease. Normal ...

Corning® Automated Cell Counters: Open vs. Closed Systems

Related Links • 3D Imaging of Optically Cleared Spheroids in Corning® Spheroid Microplates • Rock the Science of 3D – From Promise to Reality • Corning® spheroid microplates Related Products • Corning® Cell Counter • Scepter 2.0 Handheld Automated Cell Counter (60 μm) • Scepter 2.0 Handheld Automate...

EX-CELL® Advanced™ CHO Fed-batch Medium

Without L-glutamine, without hypoxanthine, and without thymidine Liquid Catalog No. 14366C Powder Catalog No. 24366C EX-CELL® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed using multivariate analysis of 10,000+ data points that ...

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

Product Number 3447-020-01 3-D Culture is an innovative approach to modeling the morphological effects of early oncogenesis on three-dimensional microenvironments. When healthy, differentiating cells exhibit a structured, polarized morphology that is critical for cellular formation and function. Dur...

Get Better Immunofluorescence Staining Images

Related Links • Antibody Explorer: Search primary and secondary antibodies • Prestige Antibodies • Antibody Resource Center Uses for immunofluorescence (IF)—where an antibody is conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-transl...

Superior Growth of MDBK Cells in Corning® CellSTACK®- 40 Culture Chambers Under Actively Gassed Conditions

Todd Upton, Elizabeth M. Martin, and Debra Hoover Corning Incoprorated, Life Sciences Kennebunk, ME 04043 Introduction We reported the enhanced performance and increased yields of Madin-Darby Bovine Kidney (MDBK) cells in CellSTACK-40 Culture Chambers relative to a similar competitor product. In tho...

Human Lung Fibroblasts (HLF) Culture Protocol

Storage Preparation for Culturing Culturing HLF Subculturing HLF Materials A. Cryopreserved Vials (506-05a, 506-05f) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen sto...

Human Epidermal Melanocytes (HEM) Culture Protocol

Storage Preparation for Culturing Culturing HEM Subculturing HEM Materials A. Cryopreserved Vials (104-05a, 104-05n) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen sto...

Cell Dissociation with Trypsin | Mechanism in cell culture

Related Content • Trypsin • Trypsin Inhibitors • Antibiotics • Antibiotic selector tool • StableCell™ Trypsin Related Products • Cell Culture Media and Reagents • Growth Factors • ECM Protein • ECACC cell lines • Cell Freezing Media and Reagents • Fetal Bovine Serum (FBS) • Human Sera • Cell Dissoci...

Cultrex® 3-D Spheroid Colorimetric Proliferation/Viability Assay Protocol

Reagent kit for investigating spheroid cell proliferation and/or viability Catalog Number 3511-096-K Current in vitro tumor models lack either a physiological context and/or reproducible format for evaluating tumor cells in vitro. At present, the most popular method for compound screening and pathwa...

Protocol: Bovine Aortic Endothelial Cells culture

Storage Preparation for Culturing Culturing BAOEC Subculturing BAOEC Materials A. Cryopreserved Vials (B304-05) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage ...

Protocol: Canine Aortic Endothelial Cells Culture

Storage Preparation for Culturing Culturing CnAOEC Subculturing CnAOEC Materials A. Cryopreserved Vials (Cn304-05) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen stora...

Protocol: Human Umbilical Artery Endothelial Cells Culture

Storage Preparation for Culturing Culturing HUAEC Subculturing HUAEC Materials A. CRYOPRESERVED VIALS (202-05n) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage ...

Cultrex® 3-D Spheroid Fluorometric Proliferation/Viability Assay Protocol

Reagent kit for investigating spheroid cell proliferation and/or viability Product Number 3510-096-K Current in vitro tumor models lack either a physiological context and/or reproducible format for evaluating tumor cells in vitro. At present, the most popular method for compound screening and pathwa...

Protocol: Bovine Pulmonary Artery Endothelial Cells Culture

Storage Preparation for Culturing Culturing BPAEC Subculturing BPAEC Materials A. Cryopreserved Vials (B302-05) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage ...

HumanKine® Thermostable bFGF

Introduction Methods – Determining stability of bFGF cytokines at elevated temperatures – Culture of human iPS cells – Expansion of ENStem™-A human neural progenitors Results Discussion Materials Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmen...

Protocol: Bovine Coronary Artery Endothelial Cells Culture

Storage Preparation for Culturing Culturing BCAEC Subculturing BCAEC Materials A. Cryopreserved Vials (B300-05) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage ...

Rat Cardiac Fibroblasts (RCF) Culture Protocol

A. Cryopreserved Vials (R306-05a, R306-05n) Store the cryovials in a liquid nitrogen storage tank immediately upon arrival. *Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room coul...