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Search term:"mouse antibody"

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387 matches found for mouse antibody

Authenticated Breast Cancer Cell Lines for Cancer Research

Related Links • Cell Culture • ECACC Cell Lines • Protocols for ECACC Cell Lines • Browse Breast Cancer Cell Lines Related Articles • Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media • Ginseng (Panax quinquefolius) Aug...

Coleus (Coleus forskohlii) | Plant Profiler

Synonyms / Common Names / Related Terms Coleon U-quinone coleus, coleonol, Coleus amboinicus Lour (CA), Coleus barbatus Benth, Coleus blumei, Coleus blumei Benth, Coleus carnosifolius, Coleus galeatus, Coleus kilimandschari, Coleus parvifolius, Coleus scutellarioides, coleus solenostemon rotundifoli...

Enzyme Explorer: Apoptosis Products

The Enzyme Explorer Apoptosis table is organized by your enzyme of interest. Under each enzyme category are listed the corresponding enzymes, substrates, inhibitors and antibodies offered by Sigma. This is only a partial list of reagents Sigma offers for apoptosis research. For additional proteins, ...

Enzyme Explorer Alkaline Phosphatase

Related Links • Find More on the Enzyme Explorer • Tissue Dissociation & Cell Detachment Enzymes • Nucleases for DNA & RNA Digestion • Proteolytic Enzymes & Inhibitors • Bovine Serum Albumin Reagents • Enzyme Substrates for Protein Detection • Cell Signaling Enzymes, Proteins and Peptides • Glycobio...

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

What are the recommended applications for KAPA SYBR® FAST One-Step qRT-PCR Kits? • Gene expression analysis • Microarray validation • Gene knockdown validation • RNAi and miRNA research What is the difference between a one-step and a two-step qRT-PCR reaction? In a one-step qRT-PCR reaction, reverse...

Duolink® PLA Troubleshooting Guide

Tips & Tricks • Upfront Considerations • Common Experimental Parameters • Sample Storage and Analysis Troubleshooting • Too High of a Background Signal • Low or Lack of Signal • Poor Imaging & Analysis Frequently Asked Questions • General Questions • Protocol and Analysis • Specific Requirements • D...

Fullerenes for Bioscience & Photovoltaic Applications

Related Products • Fullerenes • Organic Photovoltaic (OPV) Materials • p-Type Organic Semiconductors • n-Type Organic Semiconductors Related Articles • Graphene Technologies • Nanomaterials • Organic & Printed Electronics • Novel Properties and Applications of Polyhydroxylated Fullerenes • Organic P...

Cluster of Differentiation (CD) Proteins - Cytokines and Growth Factors

Cluster of Differentiation or CD are antigen markers present on the surface of leukocytes. They are important in the immune responses and are expressed by B cells, T cells, NK cells and dendritic cells. Each CD antigen binds specific antibody and elicits immune response. CD protein group now also in...

Additional Products

• Main Page • Purification & Enrichment • Protein Deglycosylation Featured Products • Additional Products for Glycoprotein Analysis • Additional Products for Phosphoprotein Analysis • Trypsin In-Gel Digest Kit • Phosphatase Inhibitor Cocktails • Chart of mass changes resulting from typical post-tran...

Ly1 antibody reactive
Synonyms ZC2HC2, ZLYAR
Species Human (55646) , Species Mouse (17089) , Species Rat (289707) , Species sheep (101103898) , Species naked mole-rat (101704670) , Species domestic guinea pig (100727750) , Species Zebrafish (393652) , Species cow (537667) , Species domestic cat (101088840) , Species dog (479095) , Species chicken (422846)

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular material organized in a highly specialized manner. It is difficult to mimic all aspects of human biology wit...

Sepharose® High Performance: Purification with High Resolution

• Use Sepharose® High Performance for intermediate or polishing steps that require high resolution (recommended flows up to 300 cm/h). Use when SOURCE™ media do not offer the required selectivity. • Use Sepharose® High Performance (34 μm particle size) HIC media for capture or scale-up when selectiv...

Antibody Support Reagents

Supporting your research doesn’t begin and end with our collection of 90,000+ antibodies alone. Antibody workflows can extend in a multitude of different ways that are bolstered by our antibody support reagents. Whether you are using our antibodies in their wide-range of validated applications or pu...

Isotyping Kits and Reagents - Antibody Characterization and Analysis

Identifying the isotype of mouse monoclonal antibodies is important for both their purification and application. IgG variants have different pH requirements for binding to Protein A and Protein G. IgM will not bind to either of these affinity agents. Protein L can be used to purify some IgG1 anti...

Use of PEPscreen® Libraries to Identify CD8 Epitopes Presented During Malaria Infection

• • Download PDF • Malaria caused by Plasmodium species kills more than one million people per year, 90% of them in sub-Saharan Africa. An understanding of immunity to this disease can contribute to the development of vaccines, which would be invaluable in eradicating this disease. Our previous work...

Performing a Purification of IgG Antibodies with MabTrap® Kit

MAbTrap® Kit contains one HiTrap® Protein G HP 1 ml column, binding, elution, and neutralization buffers, a syringe with fittings, and an optimized purification protocol (Figure 3.12). The kit contains sufficient material for up to 20 purifications of monoclonal or polyclonal IgG from serum, cell cultur...

HumanKine® Thermostable bFGF

Introduction Methods – Determining stability of bFGF cytokines at elevated temperatures – Culture of human iPS cells – Expansion of ENStem™-A human neural progenitors Results Discussion Materials Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmen...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Antibodies to Mouse Leukocytes - Antibodies for Immune Cell Signaling and Blood

Signal transduction by the T-cell antigen receptor (TCR) is initiated by a rapid and transient phosphorylation of tyrosine residues within conserved motifs (ITAMs) in the cytoplasmic tails of the CD3 subunits. These immunoreceptor tyrosine-based activation motifs (ITAMs) possess two repeats of the c...

Isotype Controls - Antibody Characterization and Analysis

Cell typing with monoclonal antibodies to CD antigens in flow cytometry or other immunological applications can give false positives in the absence of proper controls. Sigma recommends using a non-reactive immunoglobulin of the same isotype and concentration to detect non-specific binding. We offe...

Authenticated Lung Cancer Cell Lines for Cancer Research

Related Links • Cell Culture • ECACC Cell Lines • Protocols for ECACC Cell Lines • Browse Lung Cancer Cell Lines Related Articles • Non-Small Cell Lung Cancer • Lung Cancer & IHC Webinar • Breast Cancer Cell Lines • Colorectal Cancer Cell Lines • Liver Cancer Cell Lines • Pancreatic Cancer Cell Line...

ProteoQwest Colorimetric and Chemiluminescent Detection Kits for Western Blotting

ProteoQwest Colorimetric and Chemiluminescent Western Blotting Kits are designed for high sensitivity protein detection directly on Western Blot membranes. The kits include all qualified essential reagents and antibodies used in a Western blot. ProteoQwest™ Colorimetric Western Blotting Kits: • TMB ...

ELISA Procedures

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool. • Indirect ELISA • Capture ELISA • Troubleshooting Guide (PDF) • References • Reagents and Equipment • Procedure Reagents and Equipment 1. Phosphate buffered saline (PBS) tablet: 10 mM phosphate buf...

Use of PEPscreen® Peptides to Investigate CTL Class I MHC and Th Class II Responses to AAV-hF.IX Gene Therapy

• • Download PDF • Recombinant adeno-associated virus (AAV)-mediated gene therapy expressing human coagulant factor IX (hF.IX) is a promising gene transfer strategy due to the long-term expression of hF.IX in the liver and weak immune response using a liver specific promoter. However, in humans and ...

Gold Nanostructures

Related Products • Gold Nanoparticles • Silver Nanoparticles • Nanomaterials • Specialty Polymers and Nanomaterials for Drug Delivery Related Articles • Gold Nanoparticles: Properties and Applications • Silica Coated Gold Nanoparticles • Silver Nanoparticles • Silver Nanomaterials • Iron Oxide Nanop...

IsoQuick™ Kits for Mouse Monoclonal Isotyping

IsoQuick is based on immunoassay technology in the format of a lateral flow device (LFD). This technology is simple, robust and proven. Isoquick is available in three separate LFD strips for maximum test flexibility and cost effectiveness. IsoQuick develops results in five minutes or less with clear...

Hypoxia Detection Assays

Related Products • Cellular Assays • Cell Culture Media • Fetal Bovine Serum • Cell Culture Reagents • Cancer Cell Lines • Primary Cell Culture • 3D Cell Culture • Live Cell Imaging Reagents • CellASIC® Microfluidic Live Cell Imaging System • PHK Cell Labeling Dyes • Live Cell Fluorescent Dyes • Ang...

Silica-coated Gold Nanoparticles: Surface Chemistry, Properties, Benefits and Applications

In the past few decades, research and commercial use of gold nanoparticles has rapidly expanded into fields extending from biomedical engineering1 to photovoltaics.2 Most applications take advantage of the excellent optical properties of gold nanoparticles, which can be fine-tuned by varying their s...

Stem Cell Markers and Antibodies

• Embryonic Stem Cells • Adult Stem Cells • Cell Surface Pluripotency Markers • Transcription Factor Markers • Induced PSCs Markers • Germ Cell Markers (During Specification) • Ectoderm and Endoderm Markers Embryonic stem cells are found early in the development in the inner mass of blastocyst and p...

Luciferase Antibodies - Luciferase

Sigma Life Science’s polyclonal rabbit anti-luciferase is intended for use in an indirect immunofluorescent assay of cells transfected with a plasmid that incorporates the luciferase gene. If transfection is successful and the gene is expressed, the luciferase fusion protein will fluoresce. Our mou...

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra

Related Articles • Automated Purification of Antibodies Using ÄKTA Chromatography Systems • Column Packing and Preparation for Affinity Chromatography of Antibodies • Converting from Flow Velocity to Volumetric Flow Rates • Desalting and Buffer Exchange for Affinity Chromatography of Antibodies • Ge...