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913 matches found for nuclease P1

Nuclease P1 (17 KB)

Revised: 09/08/94 Page 1 of 3 Enzymatic Assay of NUCLEASE P1 (EC 3.1.30.1) RNA as Substrate PRINCIPLE: Nuclease P1 RNA + H2O + > Acid Soluble Polynucleotides Abbreviation used: RNA = Ribonucleic Acid CONDITIONS: T = 37°C, pH = 5.3, A260nm, Light path = 1 cm METHOD: Spectrophotometric Stop Rate Deter...

Nuclease P1 3amp As Substrate (20 KB)

SSADEN01 Page 1 of 4 Revised: 09/12/97 Enzymatic Assay of NUCLEASE P1 (EC 3.1.30.1) 3'-AMP as Substrate PRINCIPLE: Nuclease P1 3'-AMP + H2O > Adenosine + Pi Abbreviations used: 3'-AMP = Adenosine 3'-Monophosphate Pi = Orthophosphate CONDITIONS: T = 37°C, pH = 7.2, A660nm, Light path = 1 cm METHOD: S...

Nuclease for Research (123 KB)

Substrates Cleave nucleic acids and proteins on your own terms Whether reducing nucleic acid viscosity during protein purification or cleaving your favorite protein for analysis, our enzymes feature: • High purity for precision • Superior enzyme activity • Extensive characterization • Lot-to-lot con...

Nuclease-Free Water at Your Fingertips (275 KB)

Application Note Biomedical Key words or phrases Nucleic acids, nuclease, RNase, molecular biology, PCR, sequencing, gene editing, ultrafiltration, ultrapure water, nuclease-free water Introduction Nucleases degrade DNA and RNA by cleaving the phosphodiester bonds of the nucleic acid backbone. While...

An Application of Zinc Finger Nuclease Technology to Create Knockout Cancer Lines (982 KB )

Results Figure 3. BAX ZFN Delivery and Clone Screening ZF N- driv en deletions cause mismatches Add CEL-I Enzyme Detect break by agarose or acrylamide gel deletion wild type +Z FN wt Dilution clonin g CEL-I assay of indi vidual clones CEL-I enzyme, isolated from celery, is a nuclease that cleaves DN...

Evaluating the Effect of Chromosomal Context on Zinc Finger Nuclease Efficiency (880 KB)

Evaluating the Effect of Chromosomal Context on Zinc Finger Nuclease Efficiency Scott Bahr, Laura Cortner, Sara Ladley, Trissa Borgschulte, CHOZN ® Platform Development T eam SAFC/Sigma-Aldrich, St Louis, MO 63103 USA, scott.bahr@sial.com 50572-1 1063 safcglobal.com Conclusions and Discussion • � Th...

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases (299 KB)

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases Kathleen E. Sampson, Jim Blasberg, Tim Brayman, Amanda Brinker, Jennifer Pratt, Neetu Venkatraman, Yongling Xaio, Maureen Bourner and David C. Thompson Life Science and Technolo...

ZFN Knock-in and Knockout Cell Lines (0 KB)

An Application of Zinc Finger Nuclease Technology to Create Knock-in and Knock-out Cell Lines Fan Zhang, Nathan Zenser, Hongyi Zhang, Deborah L. Vassar, Dmitry Malkov, Suzanne Hibbs, Melissa Jahangiri, Kelly Keys, Gregory Wemhoff, and Gary Davis Cell Based Assays, Research Biotech, Sigma-Aldrich Cor...

ZFN to Create Multiple Complete Gene Knockouts (1 MB )

An Application of Zinc Finger Nuclease Technology to Create Multiple Complete Gene Knockouts in Polyploid Cancer Lines Suzanne M. Hibbs, Melissa Jahangiri, Kelly Keys, Gregory Wemhoff*, and Gary Davis Cell Based Assays, Research Biotech, Sigma-Aldrich Corporation Overview Oncogenes and cellular path...

Identification of the Putative ROSA26 Locus (793 KB )

Identification of the Putative ROSA26 Locus in Chinese Hamster Ovary Cells and Targeted Gene Integration by Zinc-Finger Nuclease (ZFN) Technology Scott Bahr, Nan Lin, Trissa Borgschulte, Jeanne Brooks, Henry George and Kevin Kayser Cell Sciences and Development, SAFC/Sigma-Aldrich, St Louis, MO 6310...

Novel Knock-in Cell Lines Development (1 MB )

Development of Novel Knock-In Cell Lines with Target Genes Endogenously Tagged by Fluorescent Reporters Utilizing Zinc Finger Nuclease Technology Deborah L. Vassar; Fan Zhang; Hongyi Zhang; Nathan Zenser; Dmitry Malkov Sigma-Aldrich Research Biotech, Cell-Based Assays/Reporter Cell Lines, 2909 Lacle...

Tagging Endogenous Genes (0 KB)

Targeted integration (creation of knock-ins) DSB No recombination Recombination with a donor NHEJ (creation of knock-outs) Targeted integration (creation of knock-ins) DSB No recombination Recombination with a donor NHEJ (creation of knock-outs) DSB No recombination Recombination with a donor NHEJ (...

Targeted Integration of Fluorescent Reporter (1 MB )

Targeted Integration of Fluorescent Reporter Genes Utilizing Zinc Finger Nucleases Deborah L. Vassar, Nathan Zenser, Hongyi Zhang, Fan Zhang, Dmitry Malkov Sigma-Aldrich Research Biotech, 2909 Laclede Ave., Saint Louis, MO 63103 USA Introduction Engineering of the mammalian genome is an area of basi...

N5661 - Datasheet (22 KB)

Nuclease S1 from Aspergillus oryzae Product Number N 5661 Storage Temperature –20 °C EC 3.1.30.1 CAS # 37288-25-8 Product Description Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-p...

N3755 - Product Information Sheet (24 KB)

Nuclease micrococcal from Staphylococcus aureus Product Number N 3755 Storage Temperature -0 ºC Product Description Enzyme Commission (EC) Number: 3.1.31.1 CAS Number: 9013-53-0 Molecular Weight: 16,807 (based on sequence) 1 This enzyme has an absolute need for Ca 2+ for activity. The pH optimum var...

N5386 - Enzyme Assay (24 KB)

N5386 Page 1 of 3 SS-DNA03 Revised: 12/21/94 ProductInformation SIGMA QUALITY CONTROL TEST PROCEDURE Enzymatic Assay of NUCLEASE, MICROCOCCAL (EC 3.1.31.1) PRINCIPLE: DNA + H2O Nuclease, Micrococcal > Acid Soluble Polynucleotides CONDITIONS: T = 37EC, pH = 8.8, A260nm, Light path = 1 cm METHOD: Spec...

CS0004 - Bulletin (127 KB)

Nuclease Micrococcal from Staphylococcus aureus with reaction buffer and BSA solution Catalog Number CS0004 Storage Temperature –20 C TECHNICAL BULLETIN CAS RN 9013-53-0 EC 3.1.31.1 Synonyms: Endonuclease micrococcal, MNase, Micrococcal endonuclease Product Description Nuclease Micrococcal is an en...

N8630 - Product Information Sheet (25 KB)

Nuclease P1 from Penicillium citrinum Product Number N 8630 Storage Temperature 2-8 °C Product Description Enzyme Commission (EC) Number: 3.1.30.1 CAS Number: 54576-84-0 Molecular Weight: 42-50 kDa 1,2 This enzyme hydrolyzes both 3’-5’ phosphodiester bonds in RNA and heat-denatured DNA, and 3’-phosp...

CSTZFN - Bulletin (1 MB)

CompoZr ® Custom Zinc Finger Nuclease (ZFN) Table of Contents Product Description 2 Procedure Overview 3 Precautions and Disclaimers 4 Storage and Stability 4 Kit Components 4 Protocols 5 Delivery of ZFNs for Gene Knockout 5 a. Nucleofection b. Electroporation c. Lipid-based d. Lentiviral transducti...

Novel Knock-in Cell Lines Utilizing ZFN (1 MB )

Development of Novel Knock-In Cell Lines with Target Genes Endogenously Tagged by Fluorescent Reporters Utilizing Zinc Finger Nuclease Technology Deborah L. Vassar; Fan Zhang; Hongyi Zhang; Nathan Zenser; Dmitry Malkov Sigma-Aldrich Research Biotech, Cell-Based Assays/Reporter Cell Lines, 2909 Lacle...

Fluorescent Reporter Genes Utilizing ZFN (1 MB )

Targeted Integration of Fluorescent Reporter Genes Utilizing Zinc Finger Nucleases Deborah L. Vassar, Nathan Zenser, Hongyi Zhang, Fan Zhang, Dmitry Malkov Sigma-Aldrich Research Biotech, 2909 Laclede Ave., Saint Louis, MO 63103 USA Introduction Engineering of the mammalian genome is an area of basi...

SAB4200752 - Datasheet (43 KB)

Anti-Nuclease P1 antibody produced in rabbit IgG fraction of antiserum Product Number SAB4200752 Product Description Anti-Nuclease P1 is produced in rabbit using Nuclease P1 from Penicillium citrinum (N8630) as the immunogen. Whole antiserum is purified using protein A immobilized on agarose to prov...

Biopak Polisher Brochure (367 KB)

Biopak ® Polisher Ultrafilter for pyrogen-, nuclease- and bacteria-free water at the point of dispense The Biopak® Polisher is a disposable ultrafiltration cartridge typically used in cell culture, biochemistry or molecular biology applications. It can be installed at the outlet of Milli-Q® water pu...

LSI 25 (8 MB )

Inside Targeted Genome Editing Using Engineered Zinc Finger Nucleases Enhancing Lentiviral Transduction Efficiency siRNA Design, Quality and Delivery: Key Drivers of Performance in the siRNA Workflow Transplex ® RNA Amplification Kit: Whole Transcriptome Amplifica-tion of Highly-Degraded RNA from FF...

Biopak Polisher Brochure (333 KB)

Biopak ® Polisher Ultrafilter for pyrogen-, nuclease- and bacteria-free water at the point of dispense The Biopak® Polisher is a disposable ultrafiltration cartridge typically used in cell culture, biochemistry or molecular biology applications. It can be installed at the outlet of Milli-Q® water pu...

MTOX1018P48 - Bulletin (158 KB)

HepaRG™ Assay Ready Plates containing HepaRG BSEP Knockout Cells Catalog Number MTOX1018P48 TECHNICAL BULLETIN Product Description CompoZr ® zinc finger nuclease (ZFN) technology is a fast and reliable way to manipulate the genome in a targeted fashion. ZFNs are naturally occurring proteins that can...

ZFNSLC35A1 - Bulletin (1 MB)

Catalog No. ZFNSLC35A1 COMPOZR ® KNOCKOUT ZFN KIT - CHO SLC35A1 Product Description CompoZr Zinc Finger Nucleases (ZFNs), a class of engineered DNA-binding proteins, facilitate targeted genome editing by binding to a user-specified locus and causing a double-strand break (DSB). The cell then employs...

MTOX1011P96 - Bulletin (157 KB)

HepaRG™ Assay Ready Plates containing HepaRG PXR Knockout Cells Catalog Number MTOX1011P96 TECHNICAL BULLETIN Product Description CompoZr ® zinc finger nuclease (ZFN) technology is a fast and reliable way to manipulate the genome in a targeted fashion. ZFNs are naturally occurring proteins that can ...

MTOX1024P24 - Bulletin (290 KB)

HepaRG Assay Ready Plates containing PERK Knockout HepaRG Cells Catalog Number MTOX1024P24 TECHNICAL BULLETIN Product Description CompoZr  zinc finger nuclease (ZFN) technology is a fast and reliable way to manipulate the genome in a targeted fashion. ZFNs are naturally occurring proteins that ca...

Transporter Function in Caco-2 Knockout Cells (390 KB)

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases Kathleen E. Sampson, Jim Blasberg, Tim Brayman, Amanda Brinker, Jennifer Pratt, Neetu Venkatraman, Yongling Xaio, Maureen Bourner and David C. Thompson Life Science and Technolo...