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377 matches found for nuclease P1

Nuclease-Free Water at Your Fingertips

Nuclease-free glassware and reagents are required for many molecular biology applications. Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this method has several drawbacks. Alternatively, an ultrafiltration cartridge can be pl...

Benzonase® Nuclease Q&A

What type of nucleic acids does Benzonase® Nuclease work on? Can I use it when isolating RNA? Benzonase Nuclease is a promiscuous endonuclease that attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular). For what purposes would I want to use Benzonase N...

Benzonase® Nuclease & Enhancers

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. More efficient than DNAse I or any other nuclease on the market, it degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective o...

Enzyme Explorer - Learning Center: Nucleases for DNA and RNA Digestion

The function of nucleases (DNases and RNases) includes the enzymatic breakdown of DNA and RNA and is necessary for numerous research applications. For example, the purification of proteins and specific nucleic acids often requires the digestion of DNA, RNA or both. Viscosity problems resulting from ...

CompoZr® Zinc Finger Nucleases FP-ZFN-IDLV

CompoZr Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate highly specific targeted editing of any genome by creating double-stranded breaks in DNA at user specified locations. You can use ZFNs to create your choice of stable genetically engineered cell lines...

CRISPR/Cas Nuclease RNA-guided Genome Editing

Order CRISPR Reagents On this page: What is CRISPR/Cas9? How does CRISPR/Cas work? How does CRISPR Cas9 work? What is CRISPR/Cas9? Our role in developing CRISPR/Cas9 CRISPR products: All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids CRISPR paired nickases Purified RNA-only guide...

Zinc Finger Nuclease (ZFN) License Agreement

This Product and its use are the subject of one or more of the following patents controlled by Sangamo BioSciences, Inc.: U.S. Patent Nos. 6,534,261, 6,607,882, 6,746,838, 6,794,136, 6,824,978, 6,866,997, 6,933,113, 6,979,539, 7,013,219, 7,030,215, 7,220,719, 7,241,573, 7,241,574, 7,585,849, 7,595,3...

Zinc Finger Nuclease - FAQS

Zinc Finger Nuclease — FAQs What is a Zinc Finger Nuclease (ZFN)? Why are these sometimes referred to as ZFN pairs? What specificity should be expected from a ZFN? Will a ZFN knockout a gene in my cell line? Will a ZFN insert a gene into my cell line? What is the best way to deliver the ZFN and the ...

CompoZr® Zinc Finger Nucleases (ZFNs) for Biological Manufacturing

We recognize that one of the most time consuming and costly endeavors biopharmaceutical companies face is creating robust, efficient cell lines. Zinc Finger Nuclease (ZFN) technology allows for easy creation of novel CHO cell lines with precise and heritable gene additions, deletions or modification...

Zinc Finger Nuclease References

Publications • Human • Rat • Mouse • Zebrafish • Plasmodium • Xenopus • Rainbow Trout • Mosquito • Pig • Rabbit • Cattle • CHO • ZFN Technology • Reviews • Posters • Label License Human Title Author / Journal Systematic identification of functional residues in mammalian histone H2AX Chen, WT. et al....

CompoZr™ Zinc Finger Nuclease (ZFN) Technology Learning Center

• What is ZFN Technology? • How do ZFNs Work? • ZFNs for Targeted Knockout Animals • ZFN References • ZFN FAQs back to ZFN homepage

Zinc Finger Nuclease Technology (ZFN) - Targeted Genome Editing for Custom Gene Knockout

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate genome editing by creating a double-stranded break in DNA at a user-specified location. A double-stranded break is important for site-specific mutagenesis in that it stimulates the cell’s natural DNA-repair p...

CompoZr® Custom ZFN (CompoZr Zinc Finger Nucleases) Service

CompoZr Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate highly specific targeted editing of any genome by creating double-stranded breaks in DNA at user specified locations. You can use ZFNs to create your choice of stable genetically engineered cell lines...

Enzymatic Assay of Nuclease S1, Product No. N5661

To standardize a procedure for the enzymatic assay of Nuclease S1, Product Number N5661 This procedure applies only to the enzymatic assay of N5661. 3.1 Purified Water – water from a deionizing system, resistivity > or = 18MΩ•cm @ 25ºC 3.2 Unit Definition – One unit will cause 1.0 microgram of singl...

What is Zinc Finger Nuclease (ZFN) Technology?

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Zinc Finger Nucleases: Highly-specific Genomic Scissors Figure 1: Each Zinc Finger Nuclease (ZFN) consists o...

Zinc Finger Nucleases (ZFNs) - How they work

Cell Line Engineering Made Simple CompoZr™ ZFNs are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections. The CompoZr protocol for cell line modification starts with the delivery of a ZFN pair into a cell line of interest, and has been designed to be ...

Zinc Finger Nuclease Efficiency

Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. Using current methods, the efficiency of targeted integration (TI) into the host genome is generally low and is highly dependent on the ...

Use of Benzonase® Nuclease to Prevent Clumping of Stored Peripheral Blood Mononuclear Cells (PBMCs) During Vaccine Evaluation

Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers Journal of Immunology (2008) 180, 1451 Maria Cecilia Huaman1,2, Laura B. Martin1, Elissa Malkin1, David L. Narum1, Louis H. Miller1, Siddhartha Mahanty1,3...

Targeted Genome Editing Using Engineered Zinc Finger Nucleases

Phil Simmons and Derek Douglas Product Management, Biotechnology Division The determination of complete genome sequences for a wide variety of experimental organisms established a first step in the search for an in-depth understanding of the complex genetic functions that define living entities. Ext...

Removing Undesirable Nucleic Acids from Samples using Nuclease Mix

Related Protocols • Applying Equilibrated Immobiline DryStrip Gels to SDS Gels • Applying Equilibrated Immobiline® DryStrip Gels • Applying Immobiline® DryStrip Gels • Cleaning Up Samples using 2-D Clean-Up Kit • Components of Rehydration Solution • Components of Sample Preparation Solutions • Desal...

Genome Engineering with CompoZr Custom Zinc Finger Nucleases (ZFNs) [VIDEO]

Genome Editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. CompoZr Zinc Finger Nucleases (ZFNs) may be used for genome editing and enable gene knockout, gene integration or gene modification for in vitro or in vivo applications by introducing...

FP-ZFNs or Fluorescent Protein-Linked Zinc Finger Nucleases

Zinc finger nucleases (ZFNs) have enabled highly efficient gene targeting in a wide variety of cell types and organisms. While CompoZr® methods for engineering highly active ZFNs have become robust and reproducible, challenges relating to ZFN delivery and expression continue to limit isolation of co...

CompoZr® Zinc Finger Nucleases

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for sitespecific mutagenesis in that they stimulate the cell's natural DN...

Ready-to-use validated zinc fingers for permanent knockout of your favorite gene

We offer ready-to-use, pre-constructed and functionally validated CompoZr Knockout Zinc Finger Nucleases (ZFNs) that are designed to permanently knockout any human, mouse or rat gene in a quick, simple and target specific manner. CompoZr Knockout ZFNs are a class of engineered DNA-binding proteins t...

Protein & Nucleic Acid Isolation - Application Index

Technical Information on Nucleases for DNA and RNA DigestionTissue homogenates or cell extracts are complex mixtures from which to purify macromolecules. Nucleic acids make crude homogenates viscous, and can bind—specifically or non-specifically—to proteins. Nucleic acids can be precipitated by al...

CompoZr™ ZFNs for Targeted Knockout Animals

Enabling Unprecedented Research in Virtually Any Species Including Rats, Mice, Rabbits, Zebrafish and More Benefits Recent Developments in Animals ZFN Knockout Animal Creation via Microinjection How to Order CompoZr Zinc Finger Nuclease (ZFN) Technology is a novel system for rapid creation of target...

Rats — The Gene Knockout You Always Wanted

Congratulations to the researchers on the publication of this groundbreaking technique in creating targeted knockouts in a rat model system. Please use the provided links to access the full text article from Science, interviews with the Director of Human and Molecular Genetics at the Medical College...

Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

CompoZr® ZFN (zinc finger nuclease) technology was used to insert the sequence for fluorescent proteins adjacent to endogenous genes for signal transduction proteins to create novel reporter cell lines for compound screening. These cell lines include ones with fluorescent tags on EGFR, STAT1, and ST...

CompoZr® Targeted Integration Kits site integration and expression

Now Available! Targeted Integration into the Rat Genome at the Rosa26 Locus CompoZr Targeted Integration Kits are designed to rapidly integrate a user-specified gene of interest (GOI) into preferred sites of integration in either the human, mouse or rat genome. In the human genome the adeno-associat...

CompoZr® Disease Models

We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development. • CompoZr Oncology Disease Model Cell Lines • CompoZr ZFN-modified ...