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41 matches found for p8465

Plant Biotech Guide (395 KB )

CelLytics CelLytic� P Plant Cell Lysis Reagent 50 mL 250 mL C 2360 F This is a non-ionic detergent-based reagent,which offers a convenient method for efficient plant cell lysis and protein solubilization. It’s a non-denaturing reagent and maintains protein immunoreactivity and biological activity. C...

Technical Article Apr01 (189 KB )

Molecular Biology Order: 1.800.325.3010 Technical Service: 1.800.325.5832 www.sigma-aldrich.com 16 Lysis of E.coli for the Purification of Soluble Recombinant Proteins using CelLytic-B TM and CelLytic-B TM II by Rick Mehigh Sigma-Aldrich Corporation, St. Louis, MO USA Introduction The first step in ...

P8465 - Datasheet (24 KB)

Protease Inhibitor Cocktail For Bacterial Cells Product Number P 8465 Storage Temperature –20 °C Product Description Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of degrading the proteins present in the extract. The best way to imp...

Protein Inhibitor Selection Guide (1.1 MB )

Coming Soon! SigmaFAST ™ Protease Inhibitor Tablet for General Use (Cat. No. S8820) Sample Type General Use Bacterial Mammalian Plant Yeast Histidine-tagged Proteins Tissue Culture Media Phosphatase Inhibitors Tyrosine/Alkaline Serine/Threonine Catalog Number P2714 / S8820 P8465 P8340 P9599 P8215 P8...

Recombinant Protein Expression (269 KB )

biomapping Recombinant Protein Expression: Extraction and Stabilization Reference and Selection Guide Efficiently extract protein Preserve protein function Prevent protein degradation biomapping Sample Type General Use Bacterial Mammalian Plant Yeast Histidine-tagged Proteins Tissue Culture Media Ph...

Cell Lysis Reference and Selection Guide (1 MB )

Efficiently Extract Protein Preserve Protein Function Prevent Protein Degradation Integrated Solutions for Recombinant Protein Extraction and Stabilization Reference and Selection Guide BACTERIAL MAMMALIAN PLANT YEAST Coming Soon! SigmaFAST ™ Protease Inhibitor Tablet for General Use (Cat. No. S8820...

C8740 - Bulletin (68 KB)

CelLytic ™ B 10× Product Code C8740 Store at Room Temperature TECHNICAL BULLETIN Product Description CelLytic™ B 10× is a proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins. CelLytic B 10× contains no buffer...

B7435 - Bulletin (65 KB)

CelLytic ™ B Product Code B7435 Store at Room Temperature TECHNICAL BULLETIN Product Description CelLytic™ B is used for the lysis of bacterial cells for the purification of recombinant and wild type proteins. CelLytic B contains 40 mM Tris-HCl, pH 8.0, and a proprietary, non-denaturing formulation ...

2019-Protease-Inhibitor-list (43 KB)

PURPLE Portfolio Protease/Phosphatase inhibitor Code Des BRAND 2019 Unit Price M-Bonus P8340-5ML PROTEASE INHIBITOR COCKTAIL FOR USE WITH Sigma 481,800 10,000 P8340-1ML PROTEASE INHIBITOR COCKTAIL FOR USE WITH Sigma 133,400 10,000 P5726-5ML PHOSPHATASE INHIBITOR COCKTAIL 2& Sigma 503,600 10,000 P004...

IP0020 - Bulletin (511 KB)

Anti-c-Myc Immunoprecipitation Kit Catalog Number IP0020 Storage Temperature 2–8 °C TECHNICAL BULLETIN Product Description The Anti-c-Myc Immunoprecipitation Kit is specially designed to allow maximal recovery of c-Myc-tagged proteins in the immunoprecipitates. The whole process is performed in mini...

Buy 1, Get 1 Free-GRCF Core Store (142 KB)

For 4 Weeks, Buy 1 Get 1 Free Buy 1 product from the list below through the GRCF Core Store and receive the same product for FREE Offer good through 10/26/2018. Cat. No. Full Product Description Core Store Price ($) Amino Acids S5897-100G Sephadex ® G-50 BioReagent, for molecular biology, DNA grade,...

IP50 - Bulletin (218 KB)

Protein G Immunoprecipitation Kit Catalog Number IP50 Storage Temperature 2–8 °C. Do Not Freeze TECHNICAL BULLETIN Product Description The Protein G Immunoprecipitation Kit is especially designed to allow maximal recovery of immuno-precipitates. The whole process is performed in mini-spin columns, i...

C5491 - Bulletin (156 KB)

CelLytic Express 1 mL Tablets Catalog Number C5491 Storage Temperature –20 C TECHNICAL BULLETIN Product Description CelLytic Express 1 mL Tablets are used to extract proteins from bacterial cells by lysis directly in the culture medium. CelLytic Express consists of a proprietary detergent and enz...

C1990 - Bulletin (213 KB)

CelLytic Express Catalog Number C1990 Storage Temperature –20 C TECHNICAL BULLETIN Product Description CelLytic Express is used to extract proteins from bacterial cells by lysis directly in the culture medium. CelLytic Express consists of a proprietary blend of a detergent and enzymes optimized f...

IP0010 - Bulletin (496 KB)

Anti-HA Immunoprecipitation Kit Catalog Number IP0010 Storage Temperature 2–8 °C TECHNICAL BULLETIN Product Description The Anti-HA Immunoprecipitation Kit is specially designed to allow maximal recovery of HA-tagged proteins in the immunoprecipitates. The whole process is performed in mini-spin col...

A2095 - Datasheet (115 KB)

Monoclonal Anti-HA-Agarose, clone HA-7 produced in mouse, purified immunoglobulin Catalog Number A2095 Storage Temperature 2-8 °C Product Description Monoclonal Anti-HA-Agarose is the immunoglobulin fraction of Monoclonal Anti-HA (mouse IgG1 isotype) covalently linked to agarose. The monoclonal anti...

C0356 - Bulletin (150 KB)

Protein Extraction Reagent Type 4 Catalog Number C0356 Store at Room Temperature TECHNICAL BULLETIN Product Description Protein Extraction Reagent Type 4 is designed to solubilize many types of proteins, including membrane proteins, during sample preparation. The solubilized proteins can be analyzed...

C0606 - Bulletin (150 KB)

Protein Extraction Reagent Type 2 Catalog Number C0606 Store at Room Temperature TECHNICAL BULLETIN Product Description Protein Extraction Reagent Type 2 is designed to solubilize many types of proteins during sample preparation. The solubilized proteins can be analyzed with two-dimensional (2D) gel...

Protease Inhibitors (212 KB)

Protect Your Proteins Protease inhibitors are valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases. During isolation and characterization, proteases may pose a threat to the fate of a protein. Four main cl...

C0481 - Bulletin (59 KB)

Protein Extraction Reagent Type 1 Product Number C0481 Store at Room Temperature TECHNICAL BULLETIN Product Description Protein Extraction Reagent Type 1 is designed to solubilize many types of proteins during sample preparation. The solubilized proteins can be analyzed in two-dimensional (2D) gel e...

Biomapping Brochure (2 MB)

Bioprecision. Introducing new levels of accuracy for all your proteomics research. biomapping Protease Inhibitor Cocktails Magnetic Beads Seppro® AQUA Peptides™ UPS Panorama® Arrays biomapping Sigma Magnetic Beads mean faster protein recovery and more accurate quantitation The magnetic properties of...

Protein Preparation Workflow Brochure (3 MB)

Preparation, Separation, Filtration & Monitoring Products REFINE PROTEIN PREPARATION. Tools for better protein analysis. The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. KEY FEATURES Unmatched Flexibility Isolate proteins from a diverse range of sample types with...

E6406 - Bulletin (97KB)

EZview™ Red Glutathione Affinity Gel Catalog Number E6406 Storage Temperature 2–8 °C; freezer safe TECHNICAL BULLETIN Product Description EZview™ Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel, designed for use in small-scale affinity capture (pull-dow...

Protein Preparation Workflow Brochure (3 MB)

Preparation, Separation, Filtration & Monitoring Products REFINE PROTEIN PREPARATION. Tools for better protein analysis. The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. KEY FEATURES Unmatched Flexibility Isolate proteins from a diverse r...

Epitope Tags in Protein Research (7 MB )

biomapping Epitope Tags in Protein Research Tag Selection & Immunotechniques Choosing an Epitope Tag . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Detection . . . . . . . . . . . . . . . . ...

(3 MB)

REFINE PROTEIN PREPARATION. Tools for better protein analysis. The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. KEY FEATURES Unmatched Flexibility Isolate proteins from a diverse range of sample types with our flexible, broad range of kits. Diverse downstream app...

F2426 - Bulletin (107KB)

EZview Red ANTI-FLAG  M2 Affinity Gel Catalog Number F2426 Storage Temperature –20 C TECHNICAL BULLETIN Product Description EZview Red ANTI-FLAG  M2 Affinity Gel is a highly visible, red-colored ANTI-FLAG M2 agarose affinity gel, designed for use in immunoprecipitation experiments. The affinity...

E5529bul. (66 KB)

EZview™ Red Streptavidin Affinity Gel Product Code E5529 Storage Temperature 2-8 °C TECHNICAL BULLETIN Product Description EZview™ Red Streptavidin Affinity Gel is a highly visible, red colored streptavidin agarose affinity gel, designed for use in small-scale affinity capture (pull-down) experiment...

E3528 - Bulletin (104KB)

EZview™ Red HIS-Select ® HC Nickel Affinity Gel Catalog Number E3528 Storage Temperature -20 °C TECHNICAL BULLETIN Product Description EZview™ Red HIS-Select ® HC Nickel Affinity Gel is a highly visible, red colored, immobilized metal-ion affinity chromatography (IMAC) resin, designed for use in sma...

PEP Guide Indices PDF (744 KB )

Catalog Number Index 229 Our Innovation, Your Research — Shaping the Future of Life Science 09830. . . . . . . . . . . . . . . . . . . . 151 14078. . . . . . . . . . . . . . . . . . . . 157 24689. . . . . . . . . . . . . . . . . . . . 157 31966. . . . . . . . . . . . . . . . . . . . 157 34668. . . ....