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26 matches found for tracrrna

SygRNA CRISPRs: Synthetic CRISPR reagents for your genome editing needs (245 KB)

5’ 3’ 3’ tracrRNA crRNA 5’ 5’ 3’ Genomic DNA Target Sequence PAM 3’ 5’ RWW 84139 1056 ©2016 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA and SIGMA-ALDRICH are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. SygRNA and Where bio begins are trademarks of Sigma-Aldri...

CRISPRPL - Bulletin (280 KB)

Custom CRISPR Plant Products Catalog Number CRISPRPL Product Description Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐associated (Cas) proteins are the components of the adaptive immune systems in bacteria and archaea for defense against infectious bacteriophages and...

Synthetic CRISPR protein (228 KB)

Genome Editing Simplified • 25% off ALL Cas9 proteins March 1 – June 31 • 100% guarantee on predesigned gRNAs • Easy online CRISPR design and ordering • Same day shipping SigmaAldrich.com/ CRISPRprotein © 2019 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. MilliporeSigma,...

ECAS9GFPPR - Bulletin (369 KB)

Recombinant Cas9-GFP and eSpCas9-GFP Fusion Proteins for Fluorophore-Assisted RNP-Based Genome Editing Cas9-GFP Protein Product Number CAS9GFPPRO eSpCas9-GFP Protein Product Number ECAS9GFPPR Storage Temperature –20 C TECHNICAL BULLETIN Product Description Fluorescent Cas9 and eSpCas9 fusion protei...

SygRNA® Synthetic Single Guide RNA (sgRNA) (288 KB)

SygRNA ® synthetic single guide RNAs are the highest quality materials currently available on the market, and are backed by the most comprehensive guarantee in the industry. Each SygRNA ® guide is custom synthesized to your specifications, including chemical modifications. They are purified by HPLC ...

FNCAS9PROT - Bulletin (394 KB)

FnCas9 Protein FnCas9 - NLS from Francisella novicida, expressed in Escherichia coli Catalog Number FNCAS9PROT Storage Temperature –20 C TECHNICAL BULLETIN Product Description FnCas9 protein is a recombinant Cas9 protein derived from a type II-B Francisella novicida Cas9 and expressed in E. coli. I...

DCAS9PROT - Bulletin (216 KB)

dCas9-3xFLAG-Biotin Protein Cas9 (D10A, H840A) - NLS-3FLAG-Biotin from Streptococcus pyogenes expressed in Escherichia coli Catalog Number DCAS9PROT Storage Temperature –20 C TECHNICAL BULLETIN Product Description dCas9-3xFLAG-Biotin is a recombinant catalytically dead Cas9 (dCas9) protein deriv...

SygRNA general datasheet (288 KB)

SygRNA ® synthetic single guide RNAs are the highest quality materials currently available on the market, and are backed by the most comprehensive guarantee in the industry. Each SygRNA ® guide is custom synthesized to your specifications, including chemical modifications. They are purified by HPLC ...

CAS9D10APR - Bulletin (345 KB)

Recombinant Cas9, eSpCas9, and Cas9D10A Nickase Proteins For RNP-Based Genome Editing Cas9 Protein, Catalog Number CAS9PROT ESPCAS9 Protein, Catalog Number ESPCAS9PRO CAS9 NICKASE, Catalog Number CAS9D10APR This product is for R&D use only, not for drug, household, or other uses. Storage Temperature...

CAS9PROT - Bulletin (345 KB)

Recombinant Cas9, eSpCas9, and Cas9D10A Nickase Proteins For RNP-Based Genome Editing Cas9 Protein, Catalog Number CAS9PROT ESPCAS9 Protein, Catalog Number ESPCAS9PRO CAS9 NICKASE, Catalog Number CAS9D10APR This product is for R&D use only, not for drug, household, or other uses. Storage Temperature...

SygRNA general datasheet (363 KB)

SygRNA ® synthetic single guide RNAs are the highest quality materials currently available on the market, and are backed by the most comprehensive guarantee in the industry. Each SygRNA ® guide is custom synthesized to your specifications, including chemical modifications. They are purified by HPLC ...

ESPCAS9PRO - Bulletin (345 KB)

Recombinant Cas9, eSpCas9, and Cas9D10A Nickase Proteins For RNP-Based Genome Editing Cas9 Protein, Catalog Number CAS9PROT ESPCAS9 Protein, Catalog Number ESPCAS9PRO CAS9 NICKASE, Catalog Number CAS9D10APR This product is for R&D use only, not for drug, household, or other uses. Storage Temperature...

SAB4200701 - Datasheet (142 KB)

Anti-CRISPR/CAS9 antibody, Mouse monoclonal clone 7A9-3A3, purified from hybridoma cell culture Product Number SAB4200701 Product Description Anti-CRISPR/CAS9 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 7A9-3A3 produced by the fusion of mouse myeloma cells and splen...

CAS9GFPPRO - Bulletin (369 KB)

Recombinant Cas9-GFP and eSpCas9-GFP Fusion Proteins for Fluorophore-Assisted RNP-Based Genome Editing Cas9-GFP Protein Product Number CAS9GFPPRO eSpCas9-GFP Protein Product Number ECAS9GFPPR Storage Temperature –20 C TECHNICAL BULLETIN Product Description Fluorescent Cas9 and eSpCas9 fusion protei...

SAB4200751 - Datasheet (142 KB)

Anti-CRISPR/Cas9 (C-terminal) antibody, Mouse monoclonal clone 10C11-A12, purified from hybridoma cell culture Product Number SAB4200751 Product Description Monoclonal Anti-CRISPR/Cas9 (C-terminal) (mouse IgG1 isotype) is derived from the hybridoma 10C11-A12 produced by the fusion of mouse myeloma c...

RNP T Cell Data Sheet (338 KB)

Genome editing experiments have never been easier or more efficient than with SygRNA ® synthetic guide RNAs and Sigma-Aldrich ® Cas9 proteins. However, some cells can be more difficult to edit than others. That’s why we have extensively validated our reagents and protocols to work in even the most c...

RNP T Cell Data Sheet (335 KB)

Genome editing experiments have never been easier or more efficient than with SygRNA ® synthetic guide RNAs and Sigma-Aldrich ® Cas9 proteins. However, some cells can be more difficult to edit than others. That’s why we have extensively validated our reagents and protocols to work in even the most c...

CRISPR Promo (622 KB)

A A A A G G G G G U U U U U C C C C C C C C U U U U G G G G A A A A A A Design CRISPR A A A A G G G G G U U U U U C C C C C C C C U U U U G G G G A A A A A A Order *Guaranteed Predesigned gRNAs A A A A G G G G G U U U U U C C C C C C C C U U U U G G G G A A A A A A Order Custom gRNAs CRISPR Core Par...

sygRNA Ms core datasheet (300 KB)

Data Sheet Model Organism Engineering with SygRNA ® Guides Mouse models are a vital tool for investigating human disease, but creating them is an expensive and labor-intensive process. SygRNA ® guides are designed to produce optimal editing results with minimum toxicity in your valuable mouse embryo...

sygRNA Ms core datasheet (290 KB)

Data Sheet Model Organism Engineering with SygRNA ® Guides Mouse models are a vital tool for investigating human disease, but creating them is an expensive and labor-intensive process. SygRNA ® guides are designed to produce optimal editing results with minimum toxicity in your valuable mouse embryo...

Cell Design Studio (2 MB)

Cell Design Studio™ Cellular Models Tailored to Customer Needs The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2 Cell Design Studio™ The research partner you can trust to deliver customized cellular models for your drug discovery and disease modeling research. C...

Cell Design Studio (2 MB)

Cell Design Studio™ Cellular Models Tailored to Customer Needs The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the U.S. and Canada. 2 Cell Design Studio™ The research partner you can trust to deliver customized cellular models for your drug discovery and dis...

sgRNA Protocol (232 KB)

SigmaAldrich.com INSTRUCTIONS FOR USE CRISPR SygRNA ® Chemically synthesized single guide RNA The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada. 2 CRISPR SygRNA ® chemically synthesized single guide RNA (sgRNA) Table of Contents Product Warra...

sgRNA Protocol (217 KB)

SigmaAldrich.com INSTRUCTIONS FOR USE CRISPR SygRNA ® Chemically synthesized single guide RNA The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2 CRISPR SygRNA ® chemically synthesized single guide RNA (sgRNA) Table of Contents Product Warranty 03 Ordering Informa...

T1706 - Bulletin (132 KB)

TransIT-CRISPR ® Transfection Reagent Catalog Number T1706 Product Description TransIT-CRISPR is a non-liposomal polymeric transfection reagent for efficient delivery of CRISPR/Cas components. TransIT-CRISPR allows for simple, fast and efficient delivery of CRISPR DNA, guide RNA and RNP (Cas9-gRNA r...

XTG360RO - Bulletin (2 MB)

R sigma-aldrich.com X-tremeGENE 360 Transfection Reagent For life science research only. Not for use in diagnostic procedures. y Version: 02 Content Version: October 2019 For transient and stable transfection of eukaryotic cells. Cat. No. 08 724 105 001 0.4 ml Cat. No. 08 724 121 001 1.0 ml Cat. No....