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12 matches found for tracrrna

Custom tracrRNA Quotation Request

Please use this form to request a quotation for custom tracrRNA synthesis. Fill out all applicable fields. If you are not sure what to enter in one or more fields, provide supporting information within the ‘Special Instructions’ text box. We will respond to your request as soon as possible. Need hel...

Webinar: SygRNA®-Synthetic Two Part CRISPR RNA System

The CRISPR/Cas genome editing system has revolutionized most every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus. Each reagent has been successful in its own right, however, each approach has limitations. SygRNA...

SygRNA ® Synthetic gRNA and sgRNA Design

What is the difference between modified and unmodified guide RNAs? Modified guide RNAs have a chemically modified backbone by adding phsphorothioate linkages to the 3' and 5' ends of the guide RNA as well as stabilizing 2'O-methyl bases whereas unmodified gRNAs do not. How do I choose between modifi...

SygRNA® Synthetic CRISPR guide RNAs

SygRNA® synthetic single guide RNAs (sgRNA), crRNAs and tracrRNAs are synthesized using our proprietary process and reagents using the highest quality materials currently available on the market. Editing activity is assured regardless of your target, cell type or application, and is backed by the mo...

Efficient Delivery of Sigma-Aldrich CRISPR via a Non-Liposomal Polymeric Transfection Reagent, TransIT-CRISPR®

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein) Complexes into two adherent cell lines, U2OS and HepaRG was achieved using TransIT-CRISPR. Sigma-Ald...

CRISPR Synthetic gRNAs, Cas9 Proteins, Controls and Vectors

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins. • CRISPR Formats • Cas9 Pr...

CRISPR/Cas Nuclease RNA-guided Genome Editing

Order CRISPR Reagents On this page: What is CRISPR/Cas9? How does CRISPR/Cas work? How does CRISPR Cas9 work? What is CRISPR/Cas9? Our role in developing CRISPR/Cas9 CRISPR products: All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids CRISPR paired nickases Purified RNA-only guide...

CRISPR Webinars [VIDEOS]

CRISPR Webinars • Primary T-Cell Editing using Nickase RNPs • New CRISPR Strategies in Visualization and Chromatin Accessibility • Cas9 GFP, Proxy CRISPR. CRISPR Chrom • SygRNA® Synthetic CRISPR • Cas9 Proteins • SAM CRISPR Activator Libraries • Sanger Whole-Genome Arrayed CRISPR Screening Library •...

Genome Editing in Plants with CRISPR/Cas9

Related Links • View Product List • CRISPR Core Partnership Program • CRISPR Use License Agreement • Agriculture Homepage • Plant Breeding Workflow Related Articles • Tips for Cell Engineering using CRISPR • A CRISPR/Cas-GFP Vector for Rapid Expression Verification • View CRISPR Webinar Successful Z...

Ribonucleoprotein (RNP) Protocols using SygRNA ® gRNAs and Cas9 proteins

Although the CRISPR system can be delivered to cells via plasmids, direct introduction of Cas9 RNP strengthens and expands the applications of CRISPR genome modification technology by eliminating the possibility of plasmid DNA integration into the host genome. RNP complexes offer faster gene editing...

CRISPR Cas9 gene editing protocol for human iPSCs

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • RNA Reprogramming Kits • Human iPSC Media • Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media Filtration • Growth Factors • CRISPR Genome Editing Basics • ...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...