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188 matches found for age

Expression of the Multiple Sclerosis-Associated MHC Class II Allele HLA-DRB1*1501 Is Regulated by Vitamin D

• • Download PDF • 1Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, 2 Department of Clinical Neurology, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom,3 Department of Medical Genetics, Division of Neurology, University of British Columbi...

Extract-N-Amp™ FFPE Tissue PCR Protocol Procedure

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol. Other DNA starting samples (PDF) may also be used. Download the full REDExtract-N-Amp™ Tissue PCR Kit Protocol (PDF) of the original protocol for a complete list. Click the image to see a researcher use the Extract-...

GenElute Bacterial Genomic DNA Kit Protocol

Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and purify genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in co...

Fragmentation of chromatin in ChIP

Fragmentation ensures that protein/DNA complexes in high–molecular-weight chromatin are soluble and accessible to your ChIP antibody. The method of fragmentation that you choose should depend on whether you performed N-ChIP or X-ChIP. If you are performing N-ChIP, then you should fragment the DNA wi...

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

Carol A. Kreader and Kevin P. Forbes One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are unreliable and there is little agreement between different algo...

LCMS Separation and Identification of 2-Hydroxyglutarates

Sigma-Aldrich has recently developed an application for the separation and identification of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LC-MS, using a CHIROBIOTIC® R Column (covalently bonded glycopeptide - ristocetin A). Chiral 2-hydroxyglutarates provide important molecular ...

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

▪ Background ▪ Reagents ▪ Procedure ▪ Materials ▪ References MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5'→3' DNA polymerase and exonucl...

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Our GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, a...

Transplex Whole Transcriptome RNA Amplifications

• • Download PDF • The efficacy of amplification of small quantities of total RNA with the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasing numbers of FACS-isolated bone marrow stem cells (10-, 100-, and 1000-cells sampl...

advanced glycosylation end product-specific receptor
Synonyms RAGE
Species Human (177) , Species Mouse (11596) , Species Rat (81722) , Species sheep (101111586) , Species domestic guinea pig (100726130) , Species naked mole-rat (101709133) , Species dog (403168) , Species cow (280986) , Species domestic cat (101096892)
Summary: The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Pediatric Cancer and IHC Webinar

Webinar Duration: 60 minutes Childhood cancer and pediatric cancer are general terms used to describe a wide range of neoplasms found in children and teenagers. Occurring in approximately 1 in 300 people under the age of 20, compared to 1 in 6 adults, pediatric cancers are more rare than adult cance...

DIG RNA Labeling Mix Protocol & Troubleshooting

Product No. 11277073910 Evaluation of DIG RNA labeling efficiency: Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction). Roche recommends loading an aliquot on ...

PerfectHyb™ Plus

Product No. H7033 Technical Bulletin No. MB-610 Store at room temperature Product Description Precautions and Disclaimer Storage/Stability Preparation Instructions Procedure Troubleshooting Guide Materials Reference PerfectHyb™ Plus hybridization buffer has been optimized to yield maximum signal wit...

A New Cancer Biomarker Discovered

Cancer is a common disease and a major cause of death in the Western world. In general, incidence rates increase with age for most cancer forms. As human populations continue to live longer due to an improvement of the general health status, cancer will affect an increasing number of individuals. A ...

Pluripotent and Multipotent Stem Cells

Related Products • Stem Cell Culture • Stem Cell Protocols • Pluripotent Stem Cell Culture • Neural Stem Cell Culture • Mesenchymal Stem Cell Culture • Hematopoietic Stem Cell Culture • Cell Culture Media and Reagents • Growth Factors • ECM Protein • Stem Cell Antibodies • CRISPR Genome Editing • 3D...

Cell Dissociation with Trypsin

Related Content • Trypsin • Trypsin Inhibitors • Antibiotics • Antibiotic selector tool • StableCell™ Trypsin Related Products • Cell Culture Media and Reagents • Growth Factors • ECM Protein • ECACC cell lines • Cell Freezing Media and Reagents • Fetal Bovine Serum (FBS) • Human Sera • Cell Dissoci...

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian biologist Metchnikoff noted people from cultures which consumed large amounts of fermented milk, gene...

Onion (Allium cepa) | Plant Profiler

Synonyms / Common Names / Related Terms Allium cepa, Allium cepa L., allium vegetables, botanicals, Liliaceae (family), onion extract, onion juice, pickling onions, white onion. Combination product example: Contractubex gel (cepae extract from onions, heparin, and allantoin). Bioactive products foun...

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

• Background • Reagents • Preparation Instructions • Storage and Stability • Procedure • Primer Hot Start • Materials • Troubleshooting Guide • References Restorase® DNA Polymerase with 10x Reaction Buffer combines Sigma's Long and Accurate enzyme technology with a DNA repair enzyme resulting in a b...

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

Introduction Protocol • Human ReNcell® VM NSC Maintenance and Passaging • Viral Infection of Human ReNcell® VM NSCs • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs • Culturing Sorted Human ReNcell® VM NSCs • 3D Matrix Cultures of Human ReNcell® VM NSCs • 3D Culture Cell Seeding of ...

MSI 测试和 IHC

By: Mike Lacey, M.D., Medical Director • MSI测试 • 历史 • 错配修复 • 遗传咨询和遗传性非息肉病直肠癌处理 • 预后 • 参考文献 结直肠癌在大多数西方国家居民中发生率普遍较高,仅次于肺癌。1虽然外科技术和化疗方面不断进步,但在过去的数十年间其预后并没有得到显著的改善。因此,这也进一步引起了人们对结直肠癌病因和致病性的关注。 尽管人们对饮食因素的致病性做了全面研究2,但疏忽了其最容易预测的风险因素,即遗传性。这可能是因为医师们对于遗传在癌症致病中的作用了解不足。3 比如说,许多内科医生认为家族性腺瘤息肉病(FAP)是唯一表征结直肠癌风险的遗传因...

RNAstable®/ RNAstable® LD Handbook

• Product Formats • Introduction • Principles and Procedures • Material needed by user • Storage • Applications • Important Notes • Protocol: Sample preparation • Protocol: Sample drying and storage • Protocol: Sample recovery • Protocol: Downstream applications • Troubleshooting Guide • Appendix A:...

Qualitative Multiplex PCR Assay for Assessing DNA Quality from FFPE Tissues and Sources of Damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.). FFPE tis...

Agricultural Contaminants

People living and working in rural areas are at risk of exposure to wide range of pollutants emitted from agricultural operations. Exposure to pesticides and herbicides is a significant concern; roughly only 85% of sprayed airborne pesticides reach their target destination [1]; the remaining 15% is ...

Certified Polymer Standards for Gel Permeation Chromatography

By Rainer Walz Knowledge of the molecular structure of polymers, in- cluding chemical composition, branching, average chain length and chain length distribution, is critical to the manufacture of many commercial products, from plas- tic bottles to canopies of fighter jets. One analytical dif- ficult...

KAPA Long Range PCR Kits FAQs

For which applications should I use KAPA Long Range PCR Kits? • PCR amplification of long targets and/or PCR using low amounts of template DNA. • Standard short- and mid-range PCR amplification. • Amplification of PCR products to be used for ligation into 3′-T-overhang cloning vectors. What is the e...

Nuclease-Free Water at Your Fingertips

Nuclease-free glassware and reagents are required for many molecular biology applications. Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this method has several drawbacks. Alternatively, an ultrafiltration cartridge can be pl...

Restriction Enzymes

Click here to return to the Molecular Biology Guide Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA. T...

Characterisation of Antibodies with BRAND® PCR Plates

Introduction The generation of fully human antibodies with unique functional properties that are exploitable for tailored therapeutic interventions remains a major challenge in the antibody technology field. However, it is possible to isolate these antibodies from human B-cellls in contact with spec...

Extract-N-Amp™ Tissue PCR Protocol

REDExtract-N-Amp™ Tissue PCR Kit Protocol, for formalin-fixed paraffin embedded sections The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol. Other DNA starting samples (49 kb PDF) may also be used. Download the full REDExtract-N-Amp™ Tissue PCR Kit Protocol (49 kb PD...

Troubleshooting PCR and RT-PCR Amplification

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 Many of the common problems with PCR and RT-PCR are identified following agarose gel electrophoresis of the reaction products. These include the absence of the expected amplification product, the presence of ...