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β-Secretase (BACE1) Activity Assay Kit: A FRET Based Assay Designed for BACE1 Inhibitor Screening

Cell Biology β Secretase (BACE1) Activity Assay Kit: A FRET Based Assay Designed for BACE1 Inhibitor Screening Lisa Prostak, Efrat Barnea, Pnina Yaish and Dorit Zharhary Sigma-Aldrich Corporation Introduction In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disea...

Listeria: a Survivor

Related Links • View Product List • Food and Beverage Home • Industrial Microbiology Testing Portfolio • Food Microbiology Testing Related Resources • ISO 11290-1:2017 for Listeria Detection • Short Talk: Compliant Listeria Monocytogenes Detection A bacteria on the rise, profiting from today’s trend...

Ginseng (Panax ginseng) | Plant Profiler

Synonyms / Common Names / Related Terms Ginseng (American ginseng, Asian ginseng, Chinese ginseng, Korean red ginseng, Panax ginseng:Panaxspp., includingP. ginsengC.C. Meyer andP. quinquefoliusL., excludingEleutherococcus senticosus), Acetylenic alcohol, acidic polysaccharides, acupuncture-moxibusti...

Chromatin Immunoprecipitation Kit CHP1 Protocol

Product Description Storage/Stability Workflow Preparation Instructions Procedure Analysis Troubleshooting Guide Frequently Asked Questions Materials References Poster & Application Note Protein-DNA interactions play critical roles in cellular functions including signal transduction, gene transcript...

Products for Angiogenesis, Antitumor Agents, Cancer Markers, Carcinogens, Chemopreventive Agents

During the last twenty years our understanding of the biology of cancer has increased dramatically. It is now widely recognized that cancer results from a series of genetic alterations causing a loss of normal growth controls, resulting in unregulated growth, lack of differentiation, apoptosis, geno...

Parkinson’s Disease: Misfolding and a Parkin Problem

Parkinson's disease (PD) is a progressive neurological disorder characterized primarily by motor symptoms that include rigidity, hypokinesia and tremor. PD is the second most common neurodegenerative disease and affects about 2-3% of the population 65 years of age and older. Located in the midbrain,...

Neural Stem Cell Culture Protocols

Related Products • Neuroscience Research • Neural Stem Cell Culture • Stem Cell Culture • Stem Cell Protocols • 3D Cell Culture • Primary Cell Culture • ECACC Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Proteins • Antibodies • Bioactive Small Mole...

Amplification of Genomic DNA using REDTaq DNA Polymerase

Background Reagents Procedure References Materials REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplification of genomic or other complex DNA templates. REDTa...

Metabolomic Profiling of Neurospora crassa Fungi

Related Articles Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell Fused-Core HPLC Columns Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical Compounds Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques Serum ...

Synonyms
Species Human (100302721)

Trending Papers

February 2017 Chimera animals have been created to both study drug effects in humans and also the goal of creating organs for use in humans. A recent breakthrough using CRISPR has occurred to knockout an animal fetus’ immune response to human cells. Now that the human cells can flourish in a non-hum...

Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial contamination raise the question which methods QC labs should apply to correctly detect microorganisms...

Western Blot Protocol | Immunoblotting Protocol

Related Links • Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Walk-away immunodetection with Immobilon GO • Western Blotting Home Page • Western Blotting Literature & Resources • Antibody Explorer: Se...

DNA Damage and Repair

Related Products • DNA Repair Regulators • DNA Repair Proteins and Reagents • Antibodies to DNA Repair Proteins Related Genes • PMS1 • MLH1 • MLH3 • MSH2 • MSH3 DNA Damage and Repair Mechanisms Damage to cellular DNA is involved in mutagenesis and the development of cancer. The DNA in a human cell u...

Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being expressed in the cells, the system can then be used for a number of different research applications and stud...

Long and Accurate PCR Amplification of DNA D8045 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1. effective denaturation of DNA template 2. adequate extension times to produce large products and 3. protection of target DNA from damage by depurination. Effective denaturation is accomplished by the use of higher temper...

GenElute HP Endotoxin-Free Maxiprep Kit Article

By Dave Cutter and Goodarz Goodarzi Sigma-Aldrich Corporation, St. Louis, MO, USA View pdf version (209 KB) On this page: • Introduction • Materials and Methods • Results and Discussion • Conclusions • Acknowledgements • References • Ordering Information Introduction Plasmid DNA purification kits th...

Rooibos (Aspalathus linearis) | Plant Profiler

Synonyms / Common Names / Related Terms Aspalathin, Aspalathus acuminatus, Aspalathus contaminata, Borbornia pinfolia, chrysoeryol, Fabaceae/Leguminosae (family), hyperoside, isoorientin, isoquercitrin, isovitexin, Kaffree tea, long life tea, luteolin, orientin, Psoralea linearis, quercetin, (R)-eri...

Pluripotent Stem Cell Culture

Interested in receiving more content like this? Sign up for our Cell Informer newsletter! Human iPS Cell Lines iPSC Reprogramming Kits Pluripotent Stem Cell Culture Media iPSC Differentiation Media iPSC Dissociation Reagents Pluripotent Stem Cell Markers MEF Feeder Cells Stem Cell Culture Protocols ...

LCMS Separation and Identification of 2-Hydroxyglutarates

Sigma-Aldrich has recently developed an application for the separation and identification of S-2-hydroxyglutaric acids and R-2-hydroxyglutaric acids by chiral LC-MS, using a CHIROBIOTIC® R Column (covalently bonded glycopeptide - ristocetin A). Chiral 2-hydroxyglutarates provide important molecular ...

SeqPlex Enhanced DNA Amplification Kit Protocol

Product Description Workflow Reagents Provided Storage/Stability Procedure Appendix 1: Optional Primer Removal Assay Troubleshooting Guide FAQs References The SeqPlex DNA Amplification Kit (SEQXE) for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from ex...

DIG RNA Labeling Kit (SP6/T7) Protocol & Troubleshooting

Product No. 11175025910 Evaluation of DIG RNA labeling efficiency: Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction). Roche recommends loading an aliquot on ...

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

Related Links • Molecular Biology Handbook • Introduction to Nucleic Acid Electrophoresis • PerfectHyb™ Plus Buffer Protocol • Bionic™ Buffer Data Introduction Southern Blotting Protocol Northern Blotting Protocol Reference The transfer of macromolecules such as nucleic acids and proteins to solid-p...

Culturing Trophoblast (TS) Cell Lines Protocol

Product lists for commonly used protocols from Cold Spring Harbor Protocols Culturing Trophoblast Stem (TS) Cell Lines Janet Rossant This protocol was adapted from "Isolation and Culture of Blastocyst-Derived Stem Cell Lines," Chapter 8 (Protocol 8, provided by the laboratory of Janet Rossant), in M...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

Pediatric Cancer and IHC Webinar

Webinar Duration: 60 minutes Childhood cancer and pediatric cancer are general terms used to describe a wide range of neoplasms found in children and teenagers. Occurring in approximately 1 in 300 people under the age of 20, compared to 1 in 6 adults, pediatric cancers are more rare than adult cance...

GenElute Bacterial Genomic DNA Kit Protocol

Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and purify genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in co...

Echinacea (Echinacea) | Plant Profiler

Synonyms / Common Names / Related Terms Alkamides, American coneflower, Asteraceae (family), black sampson, black susan, cichoric acid and polysaccharides, cock-up-hat, combflower, Echinacea angustifolia, Echinacea pallida, Echinacea Plus, Echinacea purpurea, Echinacin®, Echinacin® EC31, Echinaforce...

Nuclease-Free Water at Your Fingertips

Nuclease-free glassware and reagents are required for many molecular biology applications. Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this method has several drawbacks. Alternatively, an ultrafiltration cartridge can be pl...

Air Monitoring Paints & Coatings - Pigments

Paint pigments have been around since prehistoric early artwork and have evolved to what they are today. Pigments come in a variety of types from those that are naturally occurring mineral-based (iron oxide, clay-based, carbon black, zinc, etc) to the synthesized pigments of today. The earliest synt...

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

▪ Background ▪ Reagents ▪ Procedure ▪ Materials ▪ References MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5'→3' DNA polymerase and exonucl...