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173 matches found for age

Delivery of Nucleic Acids Using Polymers

Olivia Merkel Wayne State University, Detroit, Michigan Email: olivia.merkel@wayne.edu The medical application of molecular nanotechnology, referred to as “nanomedicine,” is believed to lead to progress in human therapeutics in terms of improving health at the molecular scale.1 This has great potent...

Hot Start Amplification of DNA with SYBR® Green JumpStart™ Taq ReadyMix™

DNA Preparation The single most important step in assuring success with PCR is high quality DNA preparation. Integrity and purity of DNA template is essential. Quantitative PCR involves multiple rounds of enzymatic reactions and is therefore more sensitive to impurities such as proteins, phenol/chlo...

Carrot (Daucus carota) | Plant Profiler

Synonyms / Common Names / Related Terms Alpha-carotene, anthocyanins, beta-carotene, carotenoid, carotenoids, carrot cake, carrot jam, carrot juice, carrot puree, carrot soup, Daucus carota, dietary fiber, grated carrots, lycopene, lycopene red carrots, myristicin, purple carrots, red carrots, Umbel...

Synthesis of Gold Nanorods: Avoiding Common Pitfalls

NanoHybrids Inc. 3913 Todd Ln #310, Austin, TX 78744, USA *Email: kimberly.homan@nanohybrids.net Plasmonic nanoparticles have unique optical properties that can be tailored to suit a variety of applications in the biotechnology1–8 and electronics9–16 industries. Gold nanorods (Figure 1), a particula...

Induced Pluripotent Stem Cell or IPS Cells FAQs

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • RNA Reprogramming Kits • Human iPSC Media • Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media Filtration • Growth Factors • Bioactive Small Molecules • Ste...

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of target DNA from damage by depurination. Effective denaturation is accomplished by the use of higher tempe...

Lymphomas and Leukemias Webinar

Leukemia and lymphoma are hematologic neoplasms that affect members of all age groups. Each year, over 140,000 people in the US are diagnosed with a hematologic malignancy of some kind. With constant advancement of treatment options, the importance of accurate diagnosis and detection of lymphomas an...

Neuropeptidases

The term "neuropeptidases" refers to those enzymes that participate in the inactivation of synaptically released neuropeptides and therefore serve to turn off the generated peptide signal. In general, these enzymes are integral plasma membrane proteinases located as ectoenzymes. Some of the enzymes ...

Culturing Trophoblast (TS) Cell Lines Protocol

Product lists for commonly used protocols from Cold Spring Harbor Protocols Culturing Trophoblast Stem (TS) Cell Lines Janet Rossant This protocol was adapted from "Isolation and Culture of Blastocyst-Derived Stem Cell Lines," Chapter 8 (Protocol 8, provided by the laboratory of Janet Rossant), in M...

Synonyms
Species Human (100302563)

Guarana (Paullinia cupana) | Plant Profiler

Synonyms / Common Names / Related Terms 1,3,7-trimethyl-2, 6-dioxopurine, 1,3,7-trimethylzanthine, Brazilian cocoa, caffeine, caffeine-tannin complex, Dark Dog Lemon®, elixir of youth, gift of the gods, Go Gum®, guarana bread, guarana gum, guarana paste, Guarana Rush®, guarana seed paste, guaranin, ...

DNA / Protein Electrophoresis and Troubleshooting Tables

• Effective Range of Separation of DNA in Polyacrylamide Gels • Effective Range of Separation of DNA in Agarose Gels • DNA Size Migration of Sample Loading Dyes • Troubleshooting Guide for SDS-PAGE Protein Electrophoresis % Acrylamide (w/v)1 Efficient Range of Separation (bp) 3.5 1000-2000 5.0 80-50...

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step. When standard cycling protocols are employed, a 0–...

Safflower (Carthamus tinctorius) | Plant Profiler

Synonyms / Common Names / Related Terms 18:2, American saffron, Asteraceae (family), bastard saffron, Carthamus tinctorius , Carthamus tinctorius L., Compositae (family), dyer's saffron, EH0202, fake saffron, false saffron, high oleic acid safflower oil, hing hua, honghua, Intralipid®, kinobeon A, l...

Air Monitoring Paints & Coatings - Pigments

Paint pigments have been around since prehistoric early artwork and have evolved to what they are today. Pigments come in a variety of types from those that are naturally occurring mineral-based (iron oxide, clay-based, carbon black, zinc, etc) to the synthesized pigments of today. The earliest synt...

Hopane Reference Standard Biomarkers from Sigma-Aldrich

An interesting application of analytical chemistry is in biomarker analysis: the use of a chemical, a set of chemicals, or chemical modifications, as clues to answering scientific questions. Sigma-Aldrich® researchers and product managers pay close attention to the needs in the biomarker industry, p...

Assay Optimization & Validation - PCR Technologies Guide

On This Page • Optimizing PCR Conditions • Validating Primer Design • Optimizing Probe Concentration • Optimizing Reaction Components and Multiplex Assays • Optimizing Mg2+ Concentration • Probe Fluorophore and Quencher Selection • Guidelines for Optimization of Quantitative Reverse Transcription PC...

LuceBridge ELISA Kit Protocol

Product Number. ABK0014 Complete kit for the systematic 3-D conformational comparability analysis of Lucentis biosimilar molecule to Ranibizumab (Lucentis trade name). Read the entire protocol before performing the assay. This kit is intended for research use only and not for use in diagnostic proce...

n-Butanol Molecular Biology Reagent

CAS RN 71-36-3 EC 200-751-6 Synonyms: 1-Butanol, Butyl Alcohol n-Butanol is useful for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation (Sambrook, p. 1.46). It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions...

Frequently Asked Questions About Chromatin Immunoprecipitation (ChIP)

For more information about ChIP experiment guides, troubleshooting tips and supplementary protocols, please view our Chromatin Immunoprecipitation (ChIP) page. Antibodies 1. Should I use a monoclonal or polyclonal antibody? Either monoclonal or polyclonal antibodies can work for ChIP. Monoclonals ar...

YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast

The yeast widely studied as a model eukaryotic system. The availability of the complete genome sequence, Saccharomyces cerevisiae is expression libraries, and deletion strains provide necessary tools for genetic screens and selections.1 Yeasts are capable of critical posttranslational modifications ...

Immunohistochemistry: Current Applications in Skin Cancer

Cutaneous Spindle Cell Tumors Cutaneous Adnexal Neoplasms Melanocytic Neoplasms Malignant Small Blue Cell Tumors Fibrohistiocytic Lesions Vascular Neoplasms Hematopoietic and Hystiocytic Tumors Infectious Agents Although immunohistochemistry techniques and applications have made great strides in the...

Glycosylation - Product Lines

Chemical Deglycosylation Enzymes for Deglycosylation & Analysis Inhibitors & Substrates Kits for Glycoprotein Analysis Labeling & Detection MALDI Analytical Tools Purification Standards & Controls Chemical Deglycosylation Product Name Product # Kits GlycoProfileTM IV, Chemical Deglycosylation Kit TF...

Applications of Percoll In Blood Cells

Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 The entire spectrum of cell types present in blood can be resolved on preformed gradients of Percoll. The method described by Pertoft et al. (55) (Fig 19) utilizes both rate zonal (separation by size) and isopycni...

KAPA3G Plant PCR Kits FAQs

What are the recommended applications for KAPA3G Plant PCR Kits? • Direct PCR from crude plant samples including leaf tissue and seed • Crude PCR from leaf tissue and seeds • Successful amplification of CTAB-extracted DNA • Streamlined workflows for transgenic screening • As a solution for improving...

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting MicroRNAs are small (22-24 nucleotide) non-coding RNA molecules that are important regulators of gene expression in a variety of eukaryotic organi...

SeqPlex Enhanced DNA Amplification Kit Protocol

Product Description Workflow Reagents Provided Storage/Stability Procedure Appendix 1: Optional Primer Removal Assay Troubleshooting Guide FAQs References The SeqPlex DNA Amplification Kit (SEQXE) for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from ex...

Exploring Colon Cancer with Prestige Antibodies®

Colorectal cancer (CRC) is one of the most common types of cancer. Each year, approximately one million new cases are detected, and approximately 600,000 deaths can be attributed to this disease worldwide. Today, surgery is the only curative treatment for colorectal cancer, but adjuvant treatment ma...

Epigenetics Overview - A portfolio of products for each step of your Epigenetic research workflow.

Epigenetics is a term coined to describe changes that are not mutation based but can still be passed on from generation to generation. Genes that are activated or repressed without any change in DNA sequence are epigenetically controlled. Epigenetic modifications are stable, but potentially reversib...

Monitoring apoptosis using four different assays on the Guava® PCATM system

Authors: Kathy Yokobata, Colleen Helsel, Larry Boyle, Jestine Ho, Katherine Gillis, and Dianne Fishwild The Guava PCA system allows you to perform multiple assays to examine cells at various stages in the progression of apoptosis. The Guava ViaCount® assay can identify cells which have entered the a...

Cultivation of Shigella

Testing for Shigella unique granulated culture media The genus Shigella consists of four species: S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. These gram-negative, non-motile and non-sporulating bacilli belong to the Enterobacteriaceae family. Genetically, they are extremely closely relate...