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Neural Stem Cell Culture Protocols

Related Products • Neuroscience Research • Neural Stem Cell Culture • Stem Cell Culture • Stem Cell Protocols • 3D Cell Culture • Primary Cell Culture • ECACC Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Proteins • Antibodies • Bioactive Small Mole...

An Investigation of Norpropoxyphene Cyclization in Methanol

• • Download PDF • • Abstract • Introduction • Observations from Norpropoxyphene Analysis by GC/MS and LC/MS • HPLC Analysis of Norpropoxyphene • LC/MS Analysis of Norpropoxyphene • LC/MS Analysis of Norpropoxyphene Amide • LC/MS Analysis of Norpropoxyphene Solution Behavior in Methanol • 1D & 2D 1H...

Cannabinoid Receptors

Cannabinoid receptors derive their name from Δ9-tetrahydrocannabinol (Δ9-THC), the psychoactive principle in Cannabis sativa (marijuana). Although marijuana has been in use for over 4,000 years as a therapeutic agent and as a recreational drug, it was not until the 1980s that evidence revealed a rec...

Research Essentials - Technical Library

Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

Gastric (Stomach) Cancer

Stomach cancer, also known as gastric cancer, is cancer developing from the lining of the stomach – wikipedia.org Gastric (stomach) cancer is a disease in which malignant (cancer) cells form in the lining of the stomach. The stomach is in the upper abdomen and helps digest food. – cancer.gov Stomach...

Carrot (Daucus carota) | Plant Profiler

Synonyms / Common Names / Related Terms Alpha-carotene, anthocyanins, beta-carotene, carotenoid, carotenoids, carrot cake, carrot jam, carrot juice, carrot puree, carrot soup, Daucus carota, dietary fiber, grated carrots, lycopene, lycopene red carrots, myristicin, purple carrots, red carrots, Umbel...

About the Sigma-Aldrich NAOSMM Program

Become the VIP of your Lab Members of the MilliporeSigma NAOSMM Program gain access to dedicated member discounts, freight savings and member only promotions. Check out some of our highlighted product areas below. Want to view your pricing? If you have already registered with MilliporeSigma to recei...

Qualitative Multiplex PCR Assay for Assessing DNA Quality from FFPE Tissues and Sources of Damaged DNA

• • Download PDF • The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aC...

The Analysis of Resveratrol in Red Wine by On-Fiber Derivatization/SPME

Related Articles • Development of a Method for Analysis of Phthalate Esters in Vegetable Oils • Chiral GC Analysis of Carvone Enantiomers in Essential Oils • SPME Method for 4-methyl-1-cyclohexanemethanol (MCHM) and propylene glycol phenyl ether (PPH) in Water Resveratrol is a phytoallexin produced ...

autophagy related 4B, cysteine peptidase
Synonyms APG4B, AUTL1
Species Human (23192) , Species Mouse (66615) , Species Rat (316640) , Species domestic guinea pig (100730562) , Species naked mole-rat (101719832) , Species sheep (101122243) , Species Zebrafish (448859) , Species cow (408002) , Species Horse (100067797) , Species domestic cat (101097334) , Species dog (609599) , Species chicken (404750)
Summary: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

DNA Ligation Kit

Product Code: LIG1 Product Description Reagents Provided Precautions and Disclaimer Storage Procedure Materials References One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology ...

Extract-N-Amp™ FFPE Tissue PCR Protocol

REDExtract-N-Amp™ Tissue PCR Kit Protocol, for formalin-fixed paraffin embedded sections Watch the video protocol Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit Video brought to you by JoVE.com Product Numbers XNATS, XNAT, and XNATR Note: The quality of DNA present in fo...

Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial contamination raise the question which methods QC labs should apply to correctly detect microorganisms...

Rooibos (Aspalathus linearis) | Plant Profiler

Synonyms / Common Names / Related Terms Aspalathin, Aspalathus acuminatus, Aspalathus contaminata, Borbornia pinfolia, chrysoeryol, Fabaceae/Leguminosae (family), hyperoside, isoorientin, isoquercitrin, isovitexin, Kaffree tea, long life tea, luteolin, orientin, Psoralea linearis, quercetin, (R)-eri...

BioFiles Volume 7, Number 2 — Neurodegenerative Diseases

BioFiles Volume 7, Number 2 — Neurodegenerative Diseases Download BioFiles v7 n2 (13.0 Mb PDF) Table of Contents • Alzheimer's Disease: Antibodies, Proteins, Peptides and Assays • Huntington's Disease: Antibodies, Proteins, and Peptides • Parkinson's Disease: Antibodies, Proteins, and Peptides Intro...

Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being expressed in the cells, the system can then be used for a number of different research applications and stud...

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute™ Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format ...

Neuropeptidases

The term "neuropeptidases" refers to those enzymes that participate in the inactivation of synaptically released neuropeptides and therefore serve to turn off the generated peptide signal. In general, these enzymes are integral plasma membrane proteinases located as ectoenzymes. Some of the enzymes ...

Technical Articles on DNA and RNA Purification

• GenElute™ HP Plasmid Kits • GenElute™ Bacterial Genomic DNA Miniprep Kit • GenElute™ Blood Genomic DNA Miniprep Kit • GenElute™ Mammalian Genomic DNA Miniprep Kit • GenElute™ Plant Genomic DNA Miniprep Kit • GenElute™ Gel Extract Kit • GenElute™ PCR Clean-Up Kit • GenElute™ Mammalian Total RNA Kit...

Prestige Antibodies® in Breast Cancer Research

• The Human Protein Atlas • Prestige Antibodies® Powered by Atlas Antibodies • Monoclonal Antibody Development • Clinical Markers (ESR1, HER2, Ki67, PGR) • Antibodies used in Breast Cancer Research • Antibodies Against Gene Products in MammaPrint, Oncotype, EndoPredict and uPA Tests • Antibodies Ide...

Current References on RNAi

Looking for current application references in the field of RNAi? Look no further. Listed below are many of the top research articles relevant to RNAi and Functional Genomics research. Articles include shRNA, lentiviral-based systems, delivery to various cell lines and transgenic animals, as well as ...

PerfectHyb™ Plus

Product No. H7033 Technical Bulletin No. MB-610 Store at room temperature Product Description Precautions and Disclaimer Storage/Stability Preparation Instructions Procedure Troubleshooting Guide Materials Reference PerfectHyb™ Plus hybridization buffer has been optimized to yield maximum signal wit...

TRANSIA® PLATE Staphylococcal Enterotoxins

Food poisoning with staphylococcal enterotoxins (SE) produced by coagulase-positive staphylococci (mainly Staphylococcus aureus) is a major health concern, causing nausea, vomiting, abdominal cramps and diarrhea. Outbreaks of SE in a variety of foods have been reported.1-4 There are seven major SE t...

Acute and Chronic Inflammation: Microglia in Neuroprotection and Neurodegeneration

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments. The inci­dence of neurodegeneration generally increases with age. However, infectious agents may also induce neuronal death via apoptosis. Years of research have link...

Additional Products

• Main Page • Purification & Enrichment • Characterization & Detection • Protein Deglycosylation Featured Products • Additional Products for Glycoprotein Analysis • Additional Products for Phosphoprotein Analysis • Trypsin In-Gel Digest Kit • Phosphatase Inhibitor Cocktails • Chart of mass changes r...

Working with PCR

Sign-up to receive the Genomics Express eNewsletter. Subscribe now! • General Considerations ○ Setting up the laboratory to avoid contamination ○ Special equipment ○ PCR reaction components ○ Thermal cycling profile for standard PCR ○ Properties of PCR enzymes • Factors to Consider in RT-PCR ○ Choic...

Turmeric, Curcumin (Curcuma longa) | Plant Profiler

Synonyms / Common Names / Related Terms Amomoum curcuma, anlatone (constituent), ar-tumerone, CUR, Curcuma, Curcuma aromatica, Curcuma aromatica Salisbury, Curcuma domestica, Curcuma domestica Valet, Curcuma longa, Curcuma longa Linn., Curcuma longa rhizoma, curcuma oil, curcumin, diferuloylmethane,...

Exploring Colon Cancer with Prestige Antibodies®

Colorectal cancer (CRC) is one of the most common types of cancer. Each year, approximately one million new cases are detected, and approximately 600,000 deaths can be attributed to this disease worldwide. Today, surgery is the only curative treatment for colorectal cancer, but adjuvant treatment ma...

Authenticated Pancreatic Cancer Cell Lines for Cancer Research

Related Links • Cell Culture • ECACC Cell Lines • Protocols for ECACC Cell Lines • Browse Pancreatic Cancer Cell Lines Related Articles • Cell-in-a-Box® • Breast Cancer Cell Lines • Colorectal Cancer Cell Lines • Liver Cancer Cell Lines • Lung Cancer Cell Lines In developed countries, pancreatic can...

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting MicroRNAs are small (22-24 nucleotide) non-coding RNA molecules that are important regulators of gene expression in a variety of eukaryotic organi...

PCR Protocol | Standard PCR Protocol

Standard PCR PCR Basics PCR In Depth PCR Historical Timeline Nucleic Acid Electrophoresis Protocols & Introduction PCR Troubleshooting Guide PCR Set-Up Considerations & Troubleshooting Popular PCR Resources PCR homepage Genomics Express newsletter Master Mix Calculator About PCR Master Mix Shop PCR ...