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134 matches found for bacterial_conjugations

Detection, quantification and visualisation of protein interactions for hit profiling by high content screening

Alongside established drug target classes, innovative approaches are addressing previously undruggable target classes such as protein–protein interactions. Protein interactions and their relocation within the cell have traditionally been studied using an over-expressed cell model or cell lysate meas...

Comprehensive solutions for your ADC – collaboration with Baxter BioPharma Solutions

SAFC and Baxter’s BioPharma Solutions business (BPS), two of the industry leading contract manufacturing organizations (CMOs) in the Antibody-Drug Conjugate (ADC) space, are offering a combined approach for your ADC to simplify the supply chain and provide comprehensive, coordinated and collaborativ...

ADC Mimic Enables LC-MS Method Development

A non-toxic Cys-conjugated ADC-mimic characterized by top-down, middle-out, and bottom-up LC-MS methods. ADCs conjugated at Cys residues of inter-chain disulfide bonds give mAbs bearing 0-4 pairs of drug-linkers. Characterizing this distribution is paramount to understanding ADC efficacy and process...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Duolink® PLA Troubleshooting Guide

Tips & Tricks • Upfront Considerations • Common Experimental Parameters • Sample Storage and Analysis Troubleshooting • Too High of a Background Signal • Low or Lack of Signal • Poor Imaging & Analysis Frequently Asked Questions • General Questions • Protocol and Analysis • Specific Requirements • D...

ADC-Mimic for LC-UV-MS Method Development

Antibody-drug conjugates (ADC) represent a rapidly emerging class of biotherapeutic molecules, which harness the specificity of monoclonal antibodies (mAb) to selectively deliver highly potent cytotoxic drugs. Like all biotherapeutics they require significant analytical characterization. A non-toxic...

Competent Cell Selection & E. coli Markers Guide

Transformation • Introduction to Bacterial Transformation • Introduction to Yeast Transformation • Introduction to Blue-white Screening • Transformation Protocols for Bacteria • Transformation Protocols for Yeast Related Resources • Molecular Biology Handbook • E. coli genotypes • Cloning & Expressi...

Duolink® PLA Multicolor Detection Protocol

Related Products • Duolink® PLA • Duolink® PLA Probemaker Guide for Multicolor Detection • PLA-Validated Primary Antibodies • Duolink® PLA Custom Services Related Articles • How to optimize the Duolink® Proximity Ligation Assay • Duolink® PLA Trouble Shooting Guide • How Proximity Ligation Assay (PL...

Next-Gen ADCs: Collaborating to Improve Supply Chains for Development and Technology Transfer with Novel Technology Platforms

Related Links • Safe Manufacture of Antibody Drug Conjugates • Antibody-Drug Conjugates (ADCs) • Baxter BioPharma Solutions • • Download PDF • Dr. Cynthia Wooge Global Segment Marketing Manager, SAFC Dr. Jyothi Swamy QC Supervisor, SAFC A look into today’s ADC landscape and how collaboration aims to...

Peptide Upload

What's available • Defining the Peptide Type • Defining the Delimiter • D-Form Amino Acid Option • Tab Delimited Data • Comma Delimited Data • Element Codes • Sequence Codes • Packaging Options • What happens after you click "Price"? Defining the Peptide Type To get started using the copy/paste func...

Stem Cell Models for Drug Discovery and Metabolomics Research

Product Manager robert.gates@sial.com Over the past three decades non-cellular and cellular systems have supplanted whole animal studies for the preliminary screening of drug candidates and well as for initial drug metabolism and toxicology studies. In 1991 inadequate metabolic and pharmacokinetic p...

NLO Materials

Introduction Crystal Grade Materials Plastic Optical Fibers References Organic NLO Materials The field of organic nonlinear optics has come a long way since the its triggering development in 1970. In that year, Davydov et al. reported a strong second harmonic generation (SHG) in organic molecules ha...

Bile Acids Kit for LC-MS

A Rapid and Reliable Kit for Bile Acids Research Bile Acids (BA) are synthesized in the liver and play important roles in cholesterol homeostasis, absorption of vitamins and lipids, and various key metabolic processes. BA analysis is an important area of study in various settings including clinical ...

Gold Nanostructures

Related Products • Gold Nanoparticles • Silver Nanoparticles • Nanomaterials • Specialty Polymers and Nanomaterials for Drug Delivery Related Articles • Gold Nanoparticles: Properties and Applications • Silica Coated Gold Nanoparticles • Silver Nanoparticles • Silver Nanomaterials • Iron Oxide Nanop...

Organic Field Effect Transistors Based on DNTT and Related Organic Semiconductors

Professor Kazuo Takimiya Emergent Molecular Function Research Group, RIKEN Center for Emergent Matter Science (CEMS), Wako, Saitama 351-0198, Japan FAX: +81-48-462-1473 Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan Introducti...

Functionalized Polyethylene Glycol (PEG) Reagents

Related Links • PEG Selection Guide The chemical modification of biologically active compounds, such as peptides, proteins, antibody fragments, aptamers, enzymes, and small molecules with polyethylene glycol (referred to as “PEGylation”), is an effective method to tailor molecular properties to part...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Understanding and Successfully Applying Materials for Dye-Sensitized Solar Cells

Hans Desilvestro, Yanek Hebting, Mikael Khan, Damion Milliken Dyesol, Queanbeyan NSW 2620, Australia Email: yhebting@dyesol.com While dye sensitization as the basis for color photography has been accepted for a very long time,1 attempts to use this principle for the conversion of solar light to elec...

Duolink® PLA Probemaker Guide for Multicolor Detection

This guide describes the use of the Duolink® PLA Multicolor Probemaker kits for the conjugation of a unique pair of PLA oligonucleotides (Red Oligos A and B, Green Oligos C and D, Orange Oligos F and G, or FarRed Oligos H and I) to a pair of primary antibodies for use in Multicolor Duolink® PLA expe...

Smart Oligonucleotide Delivery by Multifunctional Block Copolymers

Related Content • Microfluidic-Assisted Manufacturing of Materials for Nanomedicine • Three-Dimensional Bioprinting for Tissue and Disease Modeling • Nanoparticle-Based Drug and Gene Delivery for Tumor Targeting • Applications of Glucose Polymers in the Synthesis of Diagnostic and Drug Delivery Nano...

OEM and Contract Manufacturing Services

Your trusted clinical diagnostics contract manufacturing partner Key Technologies • Molecular Amplification • Oligos, probes, amidites • DNA/RNA enzymes • Molecular-grade buffers, formulations • Nucleic acid purification • Conjugation • Large molecules to beads/resins • Linkage chemistry • Detection...

ZooMAb® Antibodies Frequently Asked Questions

Related Links • View all ZooMAb® Antibody Products • ZooMAb® Antibodies • ZooMAb® Antibodies Technical Article • Antibody Explorer • Highly Cited Antibodies • Antibody Guarantee 1. Why are these called ZooMAb® antibodies? 2. How do I know what species my ZooMAb® antibody is? 3. How many species do y...

References for Labeling Antibodies

Labeling Antibodies with Alkaline Phosphatase Labeling Antibodies with Fluorochromes FITC TRITC Biotin Labeling Antibodies with Horseradish Peroxidase Glutaraldehyde Periodate Labeling Antibodies with Alkaline Phosphatase Voller, A., et al., Bull. World Health Organ., 53, 55 (1976). Harlow, E., and ...

Create Your Own PLA® Probes

Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue. Direct detection and localization of protein interactions are achieved with unparalleled specifi...

Functional Biomaterials Synthesized by Double-Head Polymerization Agents

Hua Wei1 and Suzie H. Pun2* 1Department of Chemistry, Lanzhou University, Lanzhou 730000 China 2Department of Bioengineering and Molecular Engineering and Sciences Institute University of Washington, Seattle, Washington 98195 USA *Email: spun@u.washington.edu Over the past two decades, the rapid adv...

Chaparral (Larrea divaricata) | Plant Profiler

Synonyms / Common Names / Related Terms 1 aryl tetralin lignans, chaparral taxa, chaparral tea, chaparro, creosote, creosote bush, dwarf evergreen oak, el gobernadora, falsa alcaparra, flavonoids, furanoid lignans, geroop, gobernadora, greasewood, guaiaretic acid, guamis, gumis, hediondilla, hideono...

Clean-up of Antibody Labeling Reactions Using Amicon® Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample. Use the protocol below to remove unreacted fluorescent labels using centrifugal ultrafiltration devices. Remova...

Delivery of Nucleic Acids Using Polymers

Olivia Merkel Wayne State University, Detroit, Michigan Email: olivia.merkel@wayne.edu The medical application of molecular nanotechnology, referred to as “nanomedicine,” is believed to lead to progress in human therapeutics in terms of improving health at the molecular scale.1 This has great potent...

Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18

Related Articles • Reporter US Volume 33.1 (4 Mb PDF) • Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum • Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma • LC/MS/MS Analysis of Steroid Hormones in Plasma • Chiral and Achi...

Horseradish Peroxidase (HRP) Enzymes

Peroxidase from horseradish, Enzyme Products, Peroxidase Substrates and Inhibitors Related Links • Find More on the Enzyme Explorer • Tissue Dissociation & Cell Detachment Enzymes • Nucleases for DNA & RNA Digestion • Proteolytic Enzymes & Inhibitors • Bovine Serum Albumin Reagents • Enzyme Substrat...