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314 matches found for flow_cytometry

transit-crispr-transfection-reagent

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein) Complexes into two adherent cell lines, U2OS and HepaRG was achieved using TransIT-CRISPR. Sigma-Ald...

Muse® Assays for Cell Health Analysis

In addition to using cell health assessment for elucidating disease mechanisms and therapeutic discovery, monitoring key indicators of cell health and performance helps establish uniform standards of cellular performance across long-term research studies. Knowing the performance profile of your cell...

Echinacea (Echinacea) | Plant Profiler

Synonyms / Common Names / Related Terms Alkamides, American coneflower, Asteraceae (family), black sampson, black susan, cichoric acid and polysaccharides, cock-up-hat, combflower, Echinacea angustifolia, Echinacea pallida, Echinacea Plus, Echinacea purpurea, Echinacin®, Echinacin® EC31, Echinaforce...

柠檬草(香茅)

同义词/常用名/相关术语 Abafado(葡萄牙语),α-柠檬醛,α-松油烯,Andropogon citratus,Andropogon nardus,bai mak nao(老挝语),β-柠檬醛(neral),β-月桂烯,bhustrina(印度),英国印度柠檬草,capim-cidrao,carene-2,caspase-3,Ceylon香茅草,柠檬醛,科钦柠檬香茅,Cymbopogon ambiguus, Cymbopogon citrates, Cymbopogon citratus DC, Cymbopogon excavatus, Cymbopogon flexuosus, Cy...

How to Choose a Secondary Antibody

Related Links • Antibody Explorer The following information is provided to help you decide which secondary antibody may be best for your particular application. Questions to Ask: 1. In what species was the primary antibody developed? 2. What is the class (isotype) and/or subclass of the primary anti...

Bioconjugation

Bioconjugation involves ligating molecules together in which at least one of the reactants is a biomolecule, often an antibody, protein, or oligonucleotide. Applications include attaching fluorescent probes to antibodies for flow cytometry and microscopy imaging, attaching antibodies to beads for im...

Cell Signaling & Neuroscience Assay Kits by Name

A-B C D-G H-L M-O P R-T Product Code NAME Area of Interest Platform Size Sensitivity Species Description CS0740 Acid Phosphatase Assay Kit Nitric Oxide/ Cell Stress Enzymatic 1,000 in microwell plate 0.2 mUnit mammalian Quantitative Acid Phosphatase activity measurement in tissues, whole cell extrac...

duolink pla applications

With Duolink® PLA, you can detect, quantify, and visualize protein-protein interactions, post-translational modifications, and low expression protein detection with an easy-to-execute immunodetection protocol that is similar to traditional IF or IHC experiments, but yields far more sensitive signals...

Guava® easyCyte Flow Cytometers

Guava benchtop microcapillary flow cytometers now provide up to 14 parameters of detection, with more powerful violet, blue, green, and red lasers for exceptional sensitivity to enable detection of small particles and rare signals. Video Discover the Versatility of Microcapillary Flow Cytometry Down...

duolink-troubleshooting-guide

Tips & Tricks • Upfront Considerations • Common Experimental Parameters • Sample Storage and Analysis Troubleshooting • Too High of a Background Signal • Low or Lack of Signal • Poor Imaging & Analysis Frequently Asked Questions • General Questions • Protocol and Analysis • Specific Requirements • D...

Hematopoietic Stem and Immune Cell Culture

• Hematopoietic Stem Cells • Human Monocytes • Human Mononuclear Cells (PBMCs) • Human Macrophages • Human Dendritic Cells • Stromal Cells for T-Cell Differentiation • Stem Cell Protocols Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myelo...

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody Explorer search tool to view and compare our antibodies from brands you know and trust, including Si...

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

During the first half of the 20th century, a series of scientific discoveries resolved that antibody-mediated immunity is the cornerstone of the specific immune response. Since their first use as immunolabeling research tools in the early 1970s, antibody technologies have vastly improved, and antibo...

Cell Division Monitoring using Cell Tracking Dyes

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Although there are literally thousands of publications using such dyes, some of the most commonly encounter...

Secondary Antibodies, Conjugates and Kits

Related Links • Antibody Explorer • How to Choose a Secondary Antibody Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purifi...

antibodies-in-practice-conjugated-and-secondary-antibody-uses

Conjugated Antibodies Secondary Antibodies Often for signal amplification and detection purposes, purified antibodies are conjugated to enzymes, fluorophores, or haptens, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), rhodamine, fluorescein isothiocyanate (FITC), or biotin. The var...

LuminiCell Live Cell Tracking Nanoparticles

Related Products • CellASIC Live Cell Imaging • Live Cell Imaging Reagents • Fluorescent Dyes and Probes • Lentiviral Biosensors • Live Cell Imaging Videos • Cell Culture Media • Cell Lines • Cellular Assays • Material Sciences • Nanomaterials • Antibodies Fluorescent imaging techniques with high sp...

Response Modeling

Assessing the activity of effector cell modifications in vitro is critical to refining on-target efficacy and predicting clinical response of immunotherapies. The potency of cellular therapeutics can be predicted by analyzing activation markers, antigen presentation, cytotoxicity, and the quantitati...

Assay Kits by AOI

Apoptosis/Cell Cycle Cytokines, Growth Factors and Hormones Cytoskeleton/Extracellular Matrix G Proteins Gene Regulation Intracellular Calcium Lipid Signaling Multi-Drug Resistance Neuroscience Neurotransmission Nitric Oxide/Cell Stress Protein Phosphorylation Product Code NAME Area of Interest Plat...

Vector Maps | FLAG® and 3xFLAG® Overview | System for Detection and Purification of Proteins

• FLAG® Tag Expression Vector Maps and 3xFLAG® Peptides • FLAG® and 3xFLAG® Epitope Tag DNA Sequence and Amino Acid Peptide Sequence • FLAG® and 3x FLAG® Bacterial and Mammalian Expression Vector Products • FLAG® and 3xFLAG®: A Proven System for Detection and Purification of Proteins • Highly Specif...

duolink-pla-flow-cytometry-kits

Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of protein-protein interactions with f...

Muse® Cell Analyzer

Related Links • View all Flow Cytometry Instruments • CellStream™ Benchtop Flow Cytometers • Explore how Muse® Cell Analyzer would enhance your discovery efforts Related Categories • Cell Culture • Cell Lines • Cell Dissociation Free Poster Track cell health with our new poster, illustrating pathway...

bioconjugation

Bioconjugation involves ligating molecules together in which at least one of the reactants is a biomolecule, often an antibody, protein, or oligonucleotide. Applications include attaching fluorescent probes to antibodies for flow cytometry and microscopy imaging, attaching antibodies to beads for im...

PKH Bibliography

1. Akewanlop C, Watanabe M, Singh B. et al. Phagocytosis of breast cancer cells mediated by anti-MUC- monoclonal antibody, DF3, and its bispecific antibody. Cancer Res 2001 May; 61(10):4061-5. 2. lbertine KH, Gee MH. In vivo labeling of neutrophils using a fluorescent cell linker. J Leukoc Biol 1996...

Target Discovery for Early Drug Identification

Is your lab having difficulty discovering novel targets? Our broad portfolio of assays, reagents, and libraries can help you find the right lock so you can begin the work to unlock it. From the Sanger Whole Genome CRISPR Library to Duolink® PLA to measure protein-protein interactions, and bioactive ...

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Peptide Modification Applications Acetylation Increase peptide stability by preventing N-terminal degradation Biotin Commonly used in immunoassays, histocytochemistry, and fluorescence based flow cytometry Dansyl and 2, 4-Dinitrophenyl Fluorescence based assays Fluorescein and 7-methoxycoumarin acet...

antibody-antigen-interaction

Now that you know what an antigen and antibody are, let us consider the interaction between them. The strength of interaction between antibody and antigen at single antigenic sites can be described by the affinity of the antibody for the antigen. Within each antigenic site, the variable region of th...

Serum-Free 3D Spheroid Media

Related Products • 3D Cell Culture Tools and Applications • 3D Scaffolds • 3D Hydrogels • Stem Cell Culture Basics: Media, Cell Lines and Culture Reagents • The Cancer Stem Cell Hypothesis • Stem Cell Protocols • ECACC Cell Lines • Cell Culture • Growth Factors and Cytokines • Extracellular Matrix P...

Antibodies in Practice: Proper Controls

Proper Controls The use of proper controls will help eliminate any false positive and false negative results and will enable better interpretation of the experimental data. The key to proving antibody specificity is often the correct use of controls. They will also be invaluable in troubleshooting t...

Applications of Percoll In Other Cell Types

Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 The following tables were compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type. Liver cells Species Gr...