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144 matches found for flow_cytometry

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Atto Dyes for Superior Fluorescent Imaging

• Alternatives to common Fluorophores • Reactive Atto Dyes • Atto Dye Conjugates Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridiza...

Whole Genome Amplification for Single Cell Biology - Life Science Innovations

• • Download PDF • Sigma-Aldrich Corp., St. Louis, MO, USA The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level. The secret triggers that signal human maturation, regeneration and genetic ...

Lead Discovery for Early Drug Identification

Using the correct tools can help guarantee that after you’ve identified your novel target, you can create the right key to unlock it. We can support you with the innovation you need to identify, evaluate, and rank new lead candidates in a large screen and then confirm them with secondary assays and ...

Hypoxia Detection Assays

Related Products • Cellular Assays • Cell Culture Media • Fetal Bovine Serum • Cell Culture Reagents • Cancer Cell Lines • Primary Cell Culture • 3D Cell Culture • Live Cell Imaging Reagents • CellASIC® Microfluidic Live Cell Imaging System • PHK Cell Labeling Dyes • Live Cell Fluorescent Dyes • Ang...

Detecting Neural Pathways

Mapping the sensitivity and variability of the brain is crucial for interpreting findings and driving new discoveries in neuroscience research. With innovative technologies in modulation, screening and visualization, scientists can now further their understanding of the brain. This growth of knowled...

Bioconjugation

Bioconjugation involves ligating molecules together in which at least one of the reactants is a biomolecule, often an antibody, protein, or oligonucleotide. Applications include attaching fluorescent probes to antibodies for flow cytometry and microscopy imaging, attaching antibodies to beads for im...

Hematopoietic Stem Cell Media

Hematopoietic stem cells (HSCs) represent a heterogeneous population of primitive blood progenitor cells that fall into several different classes.1,2 Within the human body, they are mainly located in the bone marrow of adults, but are also found in various fetal tissues such as umbilical cord blood,...

Duolink® PLA Resource Center

Understanding protein function in the cell can create solutions to improve human health. For that reason we created the Duolink® PLA Resource Center, to provide insightful educational resources so you become knowledgeable and stay well-informed on protein detection technology. From preliminary proce...

Applications of Percoll In Other Cell Types

Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 The following tables were compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type. Liver cells Species Gr...

Nanostructured Materials Through Ultrasonic Spray Pyrolysis | Technical Article

Jin Ho Bang,1,2 Yuri T. Didenko,3 Richard J. Helmich,1,4 and Kenneth S. Suslick1* 1School of Chemical Sciences, University of Illinois at Urbana-Champaign, 600 S. Mathews Av., Urbana, IL 61801 USA 2Current address: Department of Chemistry and Applied Chemistry, Hanyang University, 55 Hanyangdaehak-r...

A Comprehensive Workflow Real-time Analysis of Cell Migration

Amedeo Cappione, Senior Scientist | Cindy Chen, Scientist | Charles Neville, Scientist | Timothy Nadler, R&D Manager Cell migration is directed by the interaction of cells with the extracellular matrix (ECM), neighboring cells, and biomolecules present in the milieu. Cell migration contributes to bo...

Cancer Research Learning Center

• Bioactive Nutrient Explorer • Cancer Research Techniques • Carcinogen Selector • Comet Assay • Flow Cytometry • Overview of Cancer Biology • Protease Finder • PTK/PTP Expression Profiling • Protocols

Live Cell Imaging Reagents

Bring your experiments to life with live cell analysis. Analyzing cellular events in real time can lead to new biological discoveries once unachievable using traditional cellular analysis techniques. Historically, techniques such as PCR, flow cytometry and antibody immunocytochemistry staining were ...

TRC Library Citations

Publish Sooner When You Partner with the MISSION® shRNA Team! The MISSION® TRC1.5 human and mouse shRNA libraries are the proven performers for small-scale to high-throughput RNAi screening. Below you will find a selection of papers where researchers have published in peer-reviewed journals using th...

Nanostructured Materials Through Ultrasonic Spray Pyrolysis

Related Articles • A Review of Mesoprous TiO2 Thin Film • Synthesis of Melting Gels using Mono-Substituted and Di-Substituted Alkoxysiloxanes • Advanced Inorganic Materials for Solid State Lighting • Materials for Energy Efficiency (4.18 Mb PDF) • Nanoscale Surface Modification (3.24 Mb PDF) • Organ...

Cell Biology | Highest Quality and Largest Selection of Biomolecules

Enhance your understanding of biology with our world-class biomolecules. Our portfolio includes over 50,000 primary antibodies, 12,000 of which are Prestige Antibodies® powered by Atlas Antibodies, as well as a vast range of proteins, bioactive peptides, and kinases. Offering over 3,000 Bioactive Sm...

FLAG® Tag Mammalian Expression Vectors

• FLAG® Tag Expression Vector Maps and 3xFLAG® Peptides • FLAG® and 3xFLAG® Epitope Tag DNA Sequence and Amino Acid Peptide Sequence • FLAG® and 3x FLAG® Bacterial and Mammalian Expression Vector Products • FLAG® and 3xFLAG®: A Proven System for Detection and Purification of Proteins • Highly Specif...

Superior Fluorescent Imaging Using Atto Dyes

• • Download PDF • Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. The A...

LentiBrite™ for fluorescent cell imaging

Introduction Methods • Construction of lentiviral vectors encoding fluorescent protein fusions • Cell seeding and lentiviral transduction • Live cell imaging and antibody staining • Analysis of GFP-LC3 localization by flow cytometry Results Conclusion Materials Autophagy, a degradative pathway that ...

Functionalization of Fluorescent Nanodiamonds for Bioimaging

Related Products • Fluorescent Nanodiamonds • PEGs & PEOs • Nanomaterials Related Content • Nanomaterial Bioconjugation Techniques • Monodispersed Nanodiamonds & their Applications • Nanomaterials What are Fluorescent Nanodiamonds? Fluorescent nanodiamonds (FNDs) exhibit distinct properties such as ...

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Videos for the Life Scientist

  A. Abstract The Sigma Aldrich Accuspin System-Histopaque 1077 is a proven system for separating lymphocytes and other mononuclear cells from whole blood. The separated mononuclear cells can then be used for downstream applications and analysis such as flow cytometry, HLA typing, and many othe...

Tools for Biosimilars: R&D through Manufacturing

Robust analytical testing, thorough biophysical characterization, and a cost-effective strategy to scale up are the keys to accelerating the development of biosimilars. We are the only trusted industry partner that combines comprehensive technologies and the regulatory know-how to help you make the ...

Live Cell Calcium Indicators

Calcium ions (Ca2+) play vital cellular physiology roles in signal transduction pathways, in neurotransmitter release, in contraction of all muscle cell types, as enzyme cofactors, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable...

How to Choose a Secondary Antibody

Related Links • Antibody Explorer The following information is provided to help you decide which secondary antibody may be best for your particular application. Questions to Ask: 1. In what species was the primary antibody developed? 2. What is the class (isotype) and/or subclass of the primary anti...

Custom Project Flexibility

Cell Design studio has engineered a breadth of mammalian cell lines using both CRISPR and ZFN technologies. We can provide an in-house cell line or use your existing cell line to best meet your needs. In addition to immortalized lines, we have substantial experience working with human iPSCs and ESCs...

Amnis® Imaging Flow Cytometers

Combining microscopy and flow cytometry, Amnis imaging flow cytometry systems provide bright-field, dark-field (SSC), and several fluorescent images of each cell in flow, at high speed. Paired with IDEAS® software for quantitative image analysis and population statistics, the technology gives you th...

Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

Abstract Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. ...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...