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145 matches found for flow_cytometry

Bioconjugation

Bioconjugation involves ligating molecules together in which at least one of the reactants is a biomolecule, often an antibody, protein, or oligonucleotide. Applications include attaching fluorescent probes to antibodies for flow cytometry and microscopy imaging, attaching antibodies to beads for im...

Response Modeling

Assessing the activity of effector cell modifications in vitro is critical to refining on-target efficacy and predicting clinical response of immunotherapies. The potency of cellular therapeutics can be predicted by analyzing activation markers, antigen presentation, cytotoxicity, and the quantitati...

In Pursuit of the Elements

How Proximity Ligation Assay (PLA) Works

Duolink® Proximity Ligation Assay (PLA) is a powerful tool that allows in situ detection of endogenous proteins, protein modifications, and protein interactions with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution in unmodified ...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Stem Cell Culture Glossary and Terms

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • Pluripotent Stem Cell Culture • Neural Stem Cell Culture • Mesenchymal Stem Cell Culture • Hematopoietic Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media ...

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

During the first half of the 20th century, a series of scientific discoveries resolved that antibody-mediated immunity is the cornerstone of the specific immune response. Since their first use as immunolabeling research tools in the early 1970s, antibody technologies have vastly improved, and antibo...

Apoptosis Assays

Related Links • Cellular Assays • Cell Viability and Proliferation Assays • Autophagy Assays • Cell Culture Media • ECACC Authenticated Cell Lines • 3D Cell Culture • Primary Cell Culture • Stem Cell Culture • Doulink™ PLA Protein Interaction Assays • Research Antibodies • Apoptosis Inhibitors • Apo...

Cell Culture Protocol 6: Cell Counting Using a Hemocytometer

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Although there are literally thousands of publications using such dyes, some of the most commonly encounter...

Flow Cytometry Webinars

Related Links • Flow Cytometry Instruments • Amnis® Imaging Flow Cytometers • Amnis® Resources • Muse® Cell Analyzer • Guava® Flow Cytometers • CellStream™ Benchtop Flow Cytometers • Applications of imaging flow cytometry for the diagnosis of leukaemia • ToxTracker Assay for High-throughput Screenin...

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must differentiate into diverse innate and adaptive phenotypes for the body to respond to infections. In t...

Cancer Stem Cell Detection Utilizing a Red-shifted Fluorescent Live Cell Probe for Aldehyde Dehydrogenase (ALDH), AldeRed™ 588-A.

Introduction Methods Reagent Preparation Stem Cell Sample Preparation AldeRed ALDH Assay Flow Cytometry Analysis Results Materials Cancer stem cells (CSCs) are subpopulations of cancer cells that can self-renew, generate diverse cells in the tumor mass, and sustain tumorigenesis. Some researchers be...

Antibodies in Practice: Conjugated and Secondary Antibody Use

Conjugated Antibodies Secondary Antibodies Often for signal amplification and detection purposes, purified antibodies are conjugated to enzymes, fluorophores, or haptens, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), rhodamine, fluorescein isothiocyanate (FITC), or biotin. The var...

PBMC Dendritic Cell Culture Media

Dendritic Cells (DCs) are highly motile immune cells and are the most powerful antigen presenting cells (APCs) of the mammalian immune system.1 DC’s act as messengers between the innate and the adaptive immune systems by continuously sampling their environment for antigens by means of endocytosis.1 ...

T Cell Receptor Webinar

T cell biology is integral to the study of normal immune regulation as well as cancer biology, Car-T cells, epitope specificity and antigen presentation. However, primary T cells can be difficult to propagate in culture for the length of time necessary for functional assays. In addition, primary T c...

Echinacea (Echinacea) | Plant Profiler

Synonyms / Common Names / Related Terms Alkamides, American coneflower, Asteraceae (family), black sampson, black susan, cichoric acid and polysaccharides, cock-up-hat, combflower, Echinacea angustifolia, Echinacea pallida, Echinacea Plus, Echinacea purpurea, Echinacin®, Echinacin® EC31, Echinaforce...

Cloning and Expression | FLAG® and 3xFLAG® Overview

• • Download PDF • Related Pages • Expression Vectors • Tac Promoter System • Stable Expression • Transient Expression • BICEP Vectors for Bicistronic Expression • T7 Promoter System The FLAG Expression System is an established way to express, purify and detect recombinant fusion proteins. FLAG and ...

Predicting Cell Death by Rapidly Assessing Size Changes with the Scepter™ Cell Counter

Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change during apoptosis1. Recent studies have shown that this cell shrinkage is not simply a passive consequence ...

Cell Signaling & Neuroscience Assay Kits by Name

A-B C D-G H-L M-O P R-T Product Code NAME Area of Interest Platform Size Sensitivity Species Description CS0740 Acid Phosphatase Assay Kit Nitric Oxide/ Cell Stress Enzymatic 1,000 in microwell plate 0.2 mUnit mammalian Quantitative Acid Phosphatase activity measurement in tissues, whole cell extrac...

Silver Nanomaterials for Biological Applications

Steven J. Oldenburg* and Aaron E. Saunders nanoComposix, Inc., San Diego, California 92111 *Email: steve.oldenburg@nanocomposix.com Silver nanomaterials have unique physical, chemical, and optical properties that are currently being leveraged for a wide variety of biological applications. A resurgen...

Hematopoietic Stem and Immune Cell Culture

• Hematopoietic Stem Cells • Human Monocytes • Human Mononuclear Cells (PBMCs) • Human Macrophages • Human Dendritic Cells • Stromal Cells for T-Cell Differentiation • Stem Cell Protocols Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myelo...

Laboratory Support and Custom Services

Need an extra pair of hands in the lab? Experimental design can be notably challenging, encountering many unknown and time sensitive paths along the way. Count on us to be those extra pair of hands providing guidance, ideas, green chemistry options and custom consultations. Accelerate your lab perfo...

Webinar: Duolink® PLA Technology

Duolink® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technolog...

Target Identification & Validation for Early Drug Discovery

Early stages of drug discovery start with initial steps of target identification and moves to the later stages of lead optimization. Multiple sources including academic research, clinical works and commercial sector help in the identification of a suitable disease target. The chosen target is then u...

Live Cell Imaging Reagents

Bring your experiments to life with live cell analysis. Analyzing cellular events in real time can lead to new biological discoveries once unachievable using traditional cellular analysis techniques. Historically, techniques such as PCR, flow cytometry and antibody immunocytochemistry staining were ...

Cancer Research Learning Center

• Bioactive Nutrient Explorer • Cancer Research Techniques • Carcinogen Selector • Comet Assay • Flow Cytometry • Overview of Cancer Biology • Protease Finder • PTK/PTP Expression Profiling • Protocols

Guava® easyCyte Flow Cytometers

Guava benchtop microcapillary flow cytometers now provide up to 14 parameters of detection, with more powerful violet, blue, green, and red lasers for exceptional sensitivity to enable detection of small particles and rare signals. Video Discover the Versatility of Microcapillary Flow Cytometry Down...

Duolink® PLA Resource Center

Understanding protein function in the cell can create solutions to improve human health. For that reason we created the Duolink® PLA Resource Center, to provide insightful educational resources so you become knowledgeable and stay well-informed on protein detection technology. From preliminary proce...

FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), which are at a 100% concentration. However, the term cells is interchangeable with virions (kodevirions) ...

Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter

Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) cells. PBMCs are a critical component of the immune system, playing an integral role in the body’s de...