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145 matches found for flow_cytometry

Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes

Related Products • Exosome Isolation Protocol • Exosome Research Poster • Exosome Isolation Wall Chart • Live Cell Imaging Dyes • Flow Cytometry Instruments • Amnis® Imaging Flow Cytometer • Amicon® Ultra Centrifugal Filter Units • Cell Culture Media • Cancer Cell Lines • Primary Cell Culture • Stem...

MISSION® LentiPlex® Pooled shRNA Libraries - Human, Mouse, Custom

For Rapid, Convenient Genome-wide shRNA Screens [VIDEO] MISSION® LentiPlex® Pooled shRNA Library Screening in Mammalian Cells MISSION LentiPlex Pooled shRNA Libraries bring the power of the MISSION TRC shRNA collection together with our lentiviral manufacturing expertise to enable genome-wide RNAi s...

In Pursuit of the Elements

Amnis® Imaging Flow Cytometers

Combining microscopy and flow cytometry, Amnis imaging flow cytometry systems provide bright-field, dark-field (SSC), and several fluorescent images of each cell in flow, at high speed. Paired with IDEAS® software for quantitative image analysis and population statistics, the technology gives you th...

Cell Biology | Highest Quality and Largest Selection of Biomolecules

Enhance your understanding of biology with our world-class biomolecules. Our portfolio includes over 50,000 primary antibodies, 12,000 of which are Prestige Antibodies® powered by Atlas Antibodies, as well as a vast range of proteins, bioactive peptides, and kinases. Offering over 3,000 Bioactive Sm...

Cell Signaling Assays Features & Benefits

β Amyloid ELISAs Apoptosis Made Easy Kit Flow Cytometry Kit for Apoptosis Cathepsin Kits Cytochrome c Oxidase Kit Cytochrome P450 Fluorescent Detection Kits Leukotriene and Prostanoid Enzyme Immunoassay (EIA) Assays ELISA Kits FAM-FLICA Caspase Assays Caspase Activity Assays Fluorometric Cell-Associ...

Choosing Lipophilic Dye Kit for Cell Tracking

• • Download PDF • Supplemental Information Katharine A. Muirhead, Joseph D. Tario, Jr., Paul K. Wallace Table S1. Non-Perturbing Membrane-Dye Labeling Conditions for Selected Cell Types1 Cell Type Final Cell Concentration Final Dye Concentration hPBMC* (large cell number) 1 x 107 cells/mL 3 x 107 c...

Response Modeling

Assessing the activity of effector cell modifications in vitro is critical to refining on-target efficacy and predicting clinical response of immunotherapies. The potency of cellular therapeutics can be predicted by analyzing activation markers, antigen presentation, cytotoxicity, and the quantitati...

Stem Cell Culture Glossary and Terms

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • Pluripotent Stem Cell Culture • Neural Stem Cell Culture • Mesenchymal Stem Cell Culture • Hematopoietic Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media ...

Antibody Standard Validation

Antibody information Target information Species validation Application validation Storage and handling information Lot validation Citations Quality control The success of any immunodetection experiment depends on the quality of the antibodies which are employed. However, antibody reagents vary signi...

Use of PEPscreen® Libraries to Identify CD8 Epitopes Presented During Malaria Infection

• • Download PDF • Malaria caused by Plasmodium species kills more than one million people per year, 90% of them in sub-Saharan Africa. An understanding of immunity to this disease can contribute to the development of vaccines, which would be invaluable in eradicating this disease. Our previous work...

Echinacea (Echinacea) | Plant Profiler

Synonyms / Common Names / Related Terms Alkamides, American coneflower, Asteraceae (family), black sampson, black susan, cichoric acid and polysaccharides, cock-up-hat, combflower, Echinacea angustifolia, Echinacea pallida, Echinacea Plus, Echinacea purpurea, Echinacin®, Echinacin® EC31, Echinaforce...

Hematopoietic Stem Cell Culture Protocols

Isolation of Human CD34+ Hematopoietic Progenitor Cells Expansion of Human CD34+ Hematopoietic Progenitor Cells The Colony Formation Unit (CFU) Cell Assay Hematopoietic stem cells residing within mouse bone-marrow were first discovered by Till and McCulloch in the 1960s and indicated that (1) hemato...

Hematopoietic Stem and Immune Cell Culture

• Hematopoietic Stem Cells • Human Monocytes • Human Mononuclear Cells (PBMCs) • Human Macrophages • Human Dendritic Cells • Stromal Cells for T-Cell Differentiation • Stem Cell Protocols Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myelo...

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology. Various methods for monitoring cell proliferation have been developed. Counting cells using a hemocytometer is one of the simplest methods used to monitor c...

Assay Kits by AOI

Apoptosis/Cell Cycle Cytokines, Growth Factors and Hormones Cytoskeleton/Extracellular Matrix G Proteins Gene Regulation Intracellular Calcium Lipid Signaling Multi-Drug Resistance Neuroscience Neurotransmission Nitric Oxide/Cell Stress Protein Phosphorylation Product Code NAME Area of Interest Plat...

Efficient Delivery of Sigma-Aldrich CRISPR via a Non-Liposomal Polymeric Transfection Reagent, TransIT-CRISPR®

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein) Complexes into two adherent cell lines, U2OS and HepaRG was achieved using TransIT-CRISPR. Sigma-Ald...

PKH Bibliography

1. Akewanlop C, Watanabe M, Singh B. et al. Phagocytosis of breast cancer cells mediated by anti-MUC- monoclonal antibody, DF3, and its bispecific antibody. Cancer Res 2001 May; 61(10):4061-5. 2. lbertine KH, Gee MH. In vivo labeling of neutrophils using a fluorescent cell linker. J Leukoc Biol 1996...

Cloning and Expression | FLAG® and 3xFLAG® Overview

• • Download PDF • Related Pages • Expression Vectors • Tac Promoter System • Stable Expression • Transient Expression • BICEP Vectors for Bicistronic Expression • T7 Promoter System The FLAG Expression System is an established way to express, purify and detect recombinant fusion proteins. FLAG and ...

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter

Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease facing the US dairy industry1. Milk from cows with mastitis exhibits increased somatic cell counts (...

Nanostructured Materials Through Ultrasonic Spray Pyrolysis | Technical Article

Jin Ho Bang,1,2 Yuri T. Didenko,3 Richard J. Helmich,1,4 and Kenneth S. Suslick1* 1School of Chemical Sciences, University of Illinois at Urbana-Champaign, 600 S. Mathews Av., Urbana, IL 61801 USA 2Current address: Department of Chemistry and Applied Chemistry, Hanyang University, 55 Hanyangdaehak-r...

Fluorescent Microparticles and Nanobeads

Related Links • Product List • Fluorescent Silica Nanobeads • • Download PDF • Microparticles have been found to have widespread applications in medicine, biochemistry, colloid chemistry, and aerosol research. Uses include chromatographic separation media, supports for immobilized enzymes, and space...

Guava® easyCyte Flow Cytometers

Guava benchtop microcapillary flow cytometers now provide up to 14 parameters of detection, with more powerful violet, blue, green, and red lasers for exceptional sensitivity to enable detection of small particles and rare signals. Video Discover the Versatility of Microcapillary Flow Cytometry Down...

Duolink® PLA Flow Cytometry Protocol

This protocol describes the use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry. Duolink® PLA Reagents To run a Duolink® PLA experiment for detection by flow cytometry, the following ...

Human PBMC Isolation and Counting Using Scepter™ 2.0 Handheld Automated Cell Counter

Peripheral blood mononuclear cells (PBMCs) are blood cells with round nuclei, such as monocytes and lymphocytes, with the lymphocyte population comprised of T cells, B cells, and natural killer (NK) cells. PBMCs are a critical component of the immune system, playing an integral role in the body’s de...

Webinar: Duolink® PLA Technology

Duolink® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technolog...

Common Cell Culture Problems: Cell Clumping

Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in the culture medium, which occurs following cell lysis. The sticky nature of DNA causes cells and other debris to aggregate int...

Functionalization of Fluorescent Nanodiamonds for Bioimaging

Related Products • Fluorescent Nanodiamonds • PEGs & PEOs • Nanomaterials Related Content • Nanomaterial Bioconjugation Techniques • Monodispersed Nanodiamonds & their Applications • Nanomaterials What are Fluorescent Nanodiamonds? Fluorescent nanodiamonds (FNDs) exhibit distinct properties such as ...

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

Introduction Protocol • Human ReNcell® VM NSC Maintenance and Passaging • Viral Infection of Human ReNcell® VM NSCs • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs • Culturing Sorted Human ReNcell® VM NSCs • 3D Matrix Cultures of Human ReNcell® VM NSCs • 3D Culture Cell Seeding of ...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Duolink® References

Related Links • View Product List • Duolink® and PLA® Technology Duolink proximity ligation assay (PLA) has been used in countless studies in a variety of fields. Browse the following tables to view highlights from the many applications of this technology. Cancer Neuroscience Cell Biology Epigenetic...