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143 matches found for flow_cytometry

Validating the Guava ViaCount® Assay, an Automated Cell Counting/Viability Method for Use in ELISpot Assays

Many of the standard methods currently used for cell counts and viability assessments of cell suspensions, e.g., peripheral blood mononuclear cells (PBMC), do not provide very accurate or reliable measurements. The Guava PCA™ System with the ViaCount assay pro-vides enhanced sensitivity and better p...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...

Duolink® PLA Applications

With Duolink® PLA, you can detect, quantify, and visualize protein-protein interactions, post-translational modifications, and low expression protein detection with an easy-to-execute immunodetection protocol that is similar to traditional IF or IHC experiments, but yields far more sensitive signals...

What is a DNA Component?

Our plasmids are composed of both a core vector backbone and a series of DNA components. Our plasmid platform is all built around the same core backbone, which means that the DNA components within each plasmids can be interchanged into each other. Below follows a brief introduction to what we consid...

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter™ 2.0 Cell Counter

Mastitis is an inflammatory change in the mammary gland most frequently characterized by pathological changes in mammary tissue. This potentially fatal infection is the most common and costly disease facing the US dairy industry1. Milk from cows with mastitis exhibits increased somatic cell counts (...

Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection

Protein-protein interaction events Interacting proteins often function as key components in the initiation and cascade of inter-and intracellular communications and influence a large variety of cellular functions and behaviors. However, accurately identifying protein-protein interactions in fixed ti...

How Proximity Ligation Assay (PLA) Works

Duolink® Proximity Ligation Assay (PLA) is a powerful tool that allows in situ detection of endogenous proteins, protein modifications, and protein interactions with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution in unmodified ...

Cell Characterization

Our in-house R&D scientists and engineers have developed state-of-the art instruments and assays to characterize cell health, genotype, phenotype, and the immune response of genetically engineered T cells. Innovative instruments and validated assays ensure confident analysis of cell identity, purity...

Precision Genetic Modifications

Project Types Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address your scientific questi...

At-line monitoring of cell cultures: Rapid cytometric evaluation of cellular physiology

N.T. Mukwena1, F. Veraitch1, S. Santhalingham1, M. Al-Rubeai1 and P. Goix2 1Department of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK 2Guava Technologies, Inc., 25801 Industrial Blvd., Hayward, CA 94545, USA, www.guavatechnologies.com Mammalian cell culture processes face ...

TRC Library Citations

Publish Sooner When You Partner with the MISSION® shRNA Team! The MISSION® TRC1.5 human and mouse shRNA libraries are the proven performers for small-scale to high-throughput RNAi screening. Below you will find a selection of papers where researchers have published in peer-reviewed journals using th...

EMD Millipore Products Now Available

Key portions of the EMD Millipore portfolio are now available through Sigma-Aldrich.com to conveniently provide you with scientific content, selection tools and online ordering for EMD Millipore and Sigma-Aldrich products, simultaneously. Ordering EMD Millipore products on Sigma-Aldrich.com is an op...

Flow Cytometry Protocol

By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. We will be featuring different cancer research tools throughout the year, providing you with the latest techniques and products. Flow Cytometry in Cancer Research Flow cytometry a...

Key Publications and Guidance on Extracellular Vesicle Preparation

EV preparations can vary in molecular composition and function, depending on: • Cell culture viability • Apoptotic index • Cell proliferation rate • Status of the cell culture medium • Handling conditions • Other experimental parameters To support EV researchers trying to navigate this rapidly chang...

Efficient Delivery of Sigma-Aldrich CRISPR via a Non-Liposomal Polymeric Transfection Reagent, TransIT-CRISPR®

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein) Complexes into two adherent cell lines, U2OS and HepaRG was achieved using TransIT-CRISPR. Sigma-Ald...

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology. Various methods for monitoring cell proliferation have been developed. Counting cells using a hemocytometer is one of the simplest methods used to monitor c...

Assessing apoptosis in cell cultures quickly and easily using the Guava® ViaCount® assay

Authors: Kathy Yokobata, Ellen Magallanes, and Colleen Helsel When using the Guava ViaCount reagent and the Guava PCA™ instrument to count cells, cell viability is detected by differential permeation of a DNA intercalating dye. Live cells with intact membranes exclude this stain, while dead and dyin...

Extracellular Vesicle Protocol

Related Links • Extracellular Vesicle Preparation • Extracellular Vesicle Supplementary Protocols • Key Publications and Guidance on Extracellular Vesicle Preparation • Protocol Guide: Exosomes Labeling Using PKH Dyes Enrich for extracellular vesicles in just 40-60 minutes using centrifugal filters....

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recordin...

Predicting Cell Death by Rapidly Assessing Size Changes with the Scepter™ Cell Counter

Apoptosis, the process of programmed cell death, is an essential component of many biological processes. Initial studies revealed that reduction in cell volume was an early morphological change during apoptosis1. Recent studies have shown that this cell shrinkage is not simply a passive consequence ...

Common Cell Culture Problems: Cell Clumping

Cells in suspension may attach to one another and form clumps for a variety of reasons. The most common cause of cell clumping is the presence of free DNA and cell debris in the culture medium, which occurs following cell lysis. The sticky nature of DNA causes cells and other debris to aggregate int...

A Comprehensive Workflow Real-time Analysis of Cell Migration

Amedeo Cappione, Senior Scientist | Cindy Chen, Scientist | Charles Neville, Scientist | Timothy Nadler, R&D Manager Cell migration is directed by the interaction of cells with the extracellular matrix (ECM), neighboring cells, and biomolecules present in the milieu. Cell migration contributes to bo...

Benchtop flow cytometry facilitates discrimination between induction of DNA damage, oxidative stress and protein misfolding using a novel multicell GFP reporter assay

Giel Hendriks1, Remco S. Derr1, Harry Vrieling2, Robin T. Clark3 1Toxys B.V., Robert Boyleweg 4, 2333 CG, Leiden, The Netherlands 2Department of Human Genetics, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands 3MilliporeSigma, a business of Merck KGaA, Darmstadt, Germa...