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1826 matches found for HPLC

HPLC Analysis of Ciprofloxacin in Plasma on Chromolith® High Resolution RP-18e

column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001) column temp. ambient mobile phase [A] methanol; [B] 20mM phosphat buffer pH=2.5: (15:85, A:B) flow rate 3 mL/min sample Ciprofloxacin 2,4 μg/mL solved in Albumin solution. injection 3 μL detector 277 nm cell: 11 μL Featur...

HPLC Analysis of Adenosine Nucleosides on Supelcosil™ LC-18-T

column SUPELCOSIL LC-18-T, 15 cm x 4.6 mm I.D, 3 µm particles (58970-U) column temp. 30 °C mobile phase [A] 0.1 M potassium phosphate, pH 7; [B] acetonitrile gradient 0 to 2.5% B in 4 min, to 7.5% B in 2 min, to 0% B in 0.1 min, held at 0% B for 3.9 min flow rate 1 mL/min sample each compound 5 µg/m...

HPLC Analysis of Isopromethazine Enantiomers on Astec® CHIROBIOTIC® V

column CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 µm particles (11024AST) column temp. 23 °C mobile phase [A] methanol; [B] acetic acid; [C] triethylamine; (100:0.1:0.1, A:B:C) flow rate 1 mL/min sample 1 mg/mL in methanol injection 5 µL detector UV, 240 nm Categories Analytical Chromatography, Chiral Ap...

UHPLC Analysis of Riboflavin and Impurities on Titan™ C18 with MS Detection

column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U) column temp. 35 °C mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile (95:5) gradient 5% to 25% in 6 min, return to 5% b in 0.1 min, and re-equilibrate for 4 min flow rate 500 µL/min pressure 5000 psi (3...

HPLC Analysis of Cis-(+/-)-3,3a,8,8a-Tetrahydro-2H-indeno[1,2-d]oxazol-2-one Enantiomers on Astec® (R,R) P-CAP™-DP

column Astec (R,R) P-CAP-DP, 25 cm x 4.6 mm I.D., 5 µm particles (35024AST) column temp. 25 °C mobile phase [A] acetonitrile; [B] methanol; (98:2, A:B) flow rate 1 mL/min sample 1 mg/mL in methanol injection 1 µL detector UV, 230 nm Categories Analytical Chromatography, Chiral Applications, Chemical...

HPLC Analysis of Aflatoxin Analogs on Ascentis® C18

column Ascentis C18, 15 cm x 4.6 mm I.D., 3 µm particles (581322-U) column temp. 30 °C or 50 °C mobile phase [A] acetonitrile: [B] methanol: [C] water; (20:20:60, A:B:C) flow rate 1.0 mL/min sample Aflatoxin Standard Mix, diluted 1:1 with water injection 20 µL detector fluorescence, 360 nm excitatio...

HPLC Analysis of mPEG and mPEG-Peptide (Methoxy-PEG-Maleimide) on Zenix® and Zenix®-C SEC

column Zenix-C SEC-300, 30 cm x 7.8 mm, I.D., 3 µm particles (Z777092) column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777033) column temp. 25 °C mobile phase 150 mM sodium phosphate buffer, pH 7.0 flow rate 1.0 mL/min sample 20kD peptide mPEG-MAL (methoxy-PEG-Maleimide), 1 mg/mL injecti...

HPLC Analysis of Isoproterenol Enantiomers on Astec® CHIROBIOTIC® T

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST) column temp. 25 °C mobile phase [A] acetonitrile; [B] methanol; [C] acetic acid; [D] triethylamine; (55:45:0.3:0.2, A:B:C:D) flow rate 2 mL/min sample 5 mg/mL in methanol injection 2 µL detector UV, 230 nm Categories Analytical Chr...

UHPLC Analysis of SigmaMAb Universal Antibody Standard on BIOshell™ IgG 1000 Ã… C4

column BIOshell IgG 1000 Ã… C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U) column temp. 75 °C mobile phase [A] 70:30 0.1% TFA (water): 0.1% TFA (acetonitrile); [B] 50:50 0.1% TFA (water): 0.1% TFA (acetonitrile) gradient 0% B to 50% B in 50 min; 50% B to 100% B in 10 min flow rate 0.2 mL/min pr...

HPLC Analysis of cis/trans-Dothiepin Enantiomers on Astec® CYCLOBOND® I 2000 AC

column CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 µm particles (20124AST) column temp. 22 °C mobile phase [A] 1.0% triethylamine acetate, pH 4.1; [B] acetonitrile; (85:15, A:B) flow rate 1 mL/min sample 1 mg/mL in methanolpeak 1: 9.48 min.peak 2: 12.48 min. injection 2 µL detector UV, 254 nm Catego...

HPLC Analysis of p-Fluorophenyl Methyl Sulfoxide Enantiomers on Astec® CHIROBIOTIC® TAG

column CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 µm particles (14024AST) mobile phase [A] hexane; [B] ethanol; (50:50, A:B) flow rate 2 mL/min detector UV, polarimetry to confirm elution order Analysis Note Details: A. Berthod et al, J. Chromatogr A, 955 (2002) 53-69. Categories Analytical Chromatogra...

HPLC Analysis of SILu Lite Antibody Standard on BIOshell™ A400 Protein C4 Versus Competitor Column

column BIOshell A400 Protein C4, 10 cm x 2.1 mm I.D., 3.4 µm particles (66825-U) column Competitor Column, 10 cm x 2.1 mm I.D., 1.8 µm particles column temp. 75 °C mobile phase [A] 70:30 water (0.1% trifluoroacetic acid): acetonitrile (0.1% trifluoroacetic acid); [B] 50:50 water (0.1% trifluoroaceti...

HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (23 °C)

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST) column temp. 23 °C mobile phase [A] 160 mM sodium phosphate, pH 4.5; [B] ethanol; (40:60, A:B) flow rate 1 mL/min sample 1 mg/mL in methanol injection 5 µL detector UV, 205 nm Categories Analytical Chromatography, Chiral Applicatio...

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea and Fluorescence Detection

sample preparation SPE (Solid Phase Extraction) sample/matrix 25 g corn meal in 100 mL acetonitrile:water (84:16) SPE tube/cartridge Supel™ Tox AflaZea, 6 mL (55314-U) sample addition 2 mL elution collect sample in 5 mL silanized glass vial eluate post-treatment transfer 200 µL sample, add 300 µL TF...

HPLC Analysis of Antipsychotic Drugs on Ascentis® RP-Amide

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U) column temp. 35 °C mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] methanol; (40:60, A:B) flow rate 1 mL/min sample 50 µg/mL each in mobile phase injection 10 µL detector UV, 220 nm Categ...

HPLC Analysis of N-(1-Phenylethyl)maleimide Enantiomers on Astec® CHIROBIOTIC® R

column CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 µm particles (13024AST) column temp. 23 °C mobile phase [A] 0.1% triethylamine acetate, pH 4.1; [B] methanol; (70:30, A:B) flow rate 1 mL/min sample 5 mg/mL in methanol injection 3 µL detector UV, 254 nm Categories Analytical Chromatography, Chiral Applic...

HPLC Analysis of Itraconazol on Chromolith® Performance 100-3mm

sample preparation 50mg Itraconazol dissolved in 50mL Solution (5mL HCl 30% in 1L Methanol) to get the impurities. column Chromolith® Performance 100 x 3 mm (1.52001.0001) column temp. 30 °C mobile phase [A] acetonitrile; [B] methanol; [C] 1L Water + 3,4g TBAH-sulfat; (45:10:45, A:B:C) flow rate 850...

UHPLC Analysis of SIL Humira on BIOshell IgG 1000 Å C4

instrument Thermo Ultimate 3000 BioRS Column BIOshell IgG 1000 Å C4, 10 cm x 2.1 mm I.D., 2.7 µm column FPP C4, 10 cm x 2.1 mm I.D., 1.7 µm mobile phase [A] water (0.1% difluoroacetic acid); [B] acetonitrile (0.1% difluoroacetic acid) gradient 0 to 100% B in 15 min flow rate 0.3 mL/min column temp. ...

HPLC Analysis of Five Antibodies TSKgel® CM-STAT vs. Competitive Non-porous Cation Exchange Column

column TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 µm particles (821966) column temp. ambient mobile phase [A] 20 mM MES, pH 6.0; [B] 20 mM MES, 0.5 M NaCl, pH 6.0 (821966) gradient 10 to 30% B in 15 min; step to 100% B; held for 2 min; step to 10% B; held for 4 min (competive column: 10 to 30% B in 30 m...

HPLC Analysis of Cisplatin and Monohydrated Cisplatin on SeQuant ZIC-HILIC

column Sequant ZIC-HILIC, 150 x 2.1 mm, 5 µm, 200 Å mobile phase [A] 25 mM ammonium formate, pH 6.5; [B] 1,4-dioxane; (20:80, A:B) flow rate 0.1 mL/min column temp. ambient detector UV, 305 nm detector other analytical flow cell 1µL/10mm injection 1 µL Analysis Note Antineoplastic agents separated w...

HPLC Analysis of Contents of Energy Drinks on Chromolith® Performance 100-3mm

sample preparation 1ml Sample on Extrelut N1, wait 10min., eluate with 6ml Ethylacetate, blow up Ethylacetate with nitrogen, dillute sample in 1ml water. column Chromolith® Performance 100 x 3 mm (1.52001.0001) column temp. 40 °C mobile phase [A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)...

HPLC Analysis of p-Toluenesulfonic Acid (PTSA) and Pyrimethamine on SeQuant ZIC-HILIC

column SeQuant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 µm, 200 Ã… (1.50455.0001) column temp. ambient mobile phase [A] acetonitrile; [B] ammonium acetate; (90:10, A:B) flow rate 0.5 mL/min injection 1 µL detector 254 nm Featured Industry Chemicals and Industrial Polymers suitability application for HPLC

HPLC Analysis of Voriconazole Without Stress on Chromolith Performance 100-2mm

column Chromolith® Performance 100 x 2 mm (1.52006.0001) column temp. ambient mobile phase [A] acetonitrile; [B] water; (50:50, A:B) flow rate 190 μL/min pressure 302 psi (21 bar) sample Voriconazole 50 μg/mL injection 2 μL detector 256 nm (cell: 1.4 μL) Featured Industry Pharmaceutical (small molec...

HPLC Analysis of Silymarin on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U) column temp. 35 °C mobile phase [A] water with 0.1% formic acid; [B] methanol gradient held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min flow rate 0.6 mL/min pressure 4105 psi (2...

HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano

column Ascentis Cyano, 15 cm x 4.6 mm I.D., 5 µm particles (577306-U) column temp. 35 °C mobile phase [A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; (70:30, A:B) flow rate 1.0 mL/min sample 20 µg/mL in 80:12:8, water: acetonitrile: dimethylsulfoxide injection 5 µL det...

HPLC Analysis of Histidine Enantiomers on Astec® CHIROBIOTIC® T (15 °C)

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST) column temp. 15 °C mobile phase [A] water; [B] methanol; [C] formic acid; (30:70:0.006, A:B:C) flow rate 0.5 mL/min sample 200 mug /mL in 50:50 water:methanol injection 10 µL detector UV, 205 nm Categories Analytical Chromatography...

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

column Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 µm particles (53931-U) column temp. 30 °C mobile phase methanol flow rate 1.0 mL/min pressure 1653 psi (114 bar) sample 0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol injection 5 µL detector UV, 2...

HPLC Analysis Report of Aflatoxins on Ascentis® Express C18

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U) column temp. 35 °C mobile phase [A] 5 mM ammonium formate, pH 2.5 (titrated with formic acid); [B] methanol gradient 5 to 100% B in 10 min flow rate 0.4 mL/min pressure 4714 psi (325 bar) sample 0.15 - 30 mg/L in 90:10, wat...

HPLC Analysis of Antipsychotic Drugs on Ascentis® Express RP-Amide

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U) column temp. 35 °C mobile phase [A] 5 mM ammonium formate (in water); [B] 5 mM ammonium formate (in 95:5 methanol:water) flow rate 3 mL/min sample 200 µg/L in water injection 1 µL detector ESI(+), TIC 100 - 1000 m/z Ca...

HPLC Analysis of Sotalol Enantiomers on Astec® CHIROBIOTIC® T

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST) column temp. 25 °C mobile phase 15 mM ammonium formate in methanol flow rate 1 mL/min sample 1 mg/mL in methanol injection 5 µL detector UV, 220 nm Categories Analytical Chromatography, Chiral Applications, Pharmaceutical (small mo...