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PerfectHyb™ Plus

Product No. H7033 Technical Bulletin No. MB-610 Store at room temperature Product Description Precautions and Disclaimer Storage/Stability Preparation Instructions Procedure Troubleshooting Guide Materials Reference PerfectHyb™ Plus hybridization buffer has been optimized to yield maximum signal wit...

Troubleshooting PCR and RT-PCR Amplification

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 Many of the common problems with PCR and RT-PCR are identified following agarose gel electrophoresis of the reaction products. These include the absence of the expected amplification product, the presence of ...

Research Essentials - Technical Library

Buffer pKa/pH Tables and Formulas: Useful pH Ranges of Selected Biological Buffers (25 °C, 0.1M) Trizma Buffer Table - pH vs. Temperature Phosphate Buffer Table - 0.2M solution Commonly Used Electrophoresis Buffers Helpful Formulas DNA / Protein Electrophoresis and Troubleshooting Tables: Effective ...

RNAlater Storage Solution for DNA & RNA Purification

Description: RNAlater™ is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing. RNAlater ca...

DNA / Protein Electrophoresis and Troubleshooting Tables

• Effective Range of Separation of DNA in Polyacrylamide Gels • Effective Range of Separation of DNA in Agarose Gels • DNA Size Migration of Sample Loading Dyes • Troubleshooting Guide for SDS-PAGE Protein Electrophoresis % Acrylamide (w/v)1 Efficient Range of Separation (bp) 3.5 1000-2000 5.0 80-50...

Glycoprotein Analysis

• Main Page • Purification & Enrichment • Characterization & Detection • Protein Deglycosylation Featured Products • Additional Products for Glycoprotein Analysis • Additional Products for Phosphoprotein Analysis • Trypsin In-Gel Digest Kit • Phosphatase Inhibitor Cocktails • Chart of mass changes r...

An Investigation of Norpropoxyphene Cyclization in Methanol

• • Download PDF • • Abstract • Introduction • Observations from Norpropoxyphene Analysis by GC/MS and LC/MS • HPLC Analysis of Norpropoxyphene • LC/MS Analysis of Norpropoxyphene • LC/MS Analysis of Norpropoxyphene Amide • LC/MS Analysis of Norpropoxyphene Solution Behavior in Methanol • 1D & 2D 1H...

Nucleic Acid Electrophoresis Protocols & Introduction

Related Links • Molecular Biology Handbook • Electrophoresis & Hybridization • Agarose Learning Center Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference Electrophoresis is a method of separation and purification ...

Seppro® References

Title Author / Journal Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery Guryča et al. Biology . Mar 2014; 3(1): 205–219. The Serum Proteome of Non-Alcoholic Fatty Liver Disease - A multimodal approach to discovery of biomarkers of Non-Alcoho...

advanced glycosylation end product-specific receptor
Synonyms RAGE
Species Human (177) , Species Mouse (11596) , Species Rat (81722) , Species sheep (101111586) , Species domestic guinea pig (100726130) , Species naked mole-rat (101709133) , Species dog (403168) , Species cow (280986) , Species domestic cat (101096892)
Summary: The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

New Cancer Biomarker | BioFiles Volume 5 Number 6 - Cancer

BioFiles Volume 5, Number 6 — Cancer Download BioFiles v5 n6 (3.38 Mb PDF) Table of Contents • Introduction • New Cancer Biomarker Breast Cancer Malignant Melanoma Epithelial Ovarian Cancer Colon Cancer Summary • Cancer Research: Epigentic Mechanisms • Folic Acid Metabolism: A Role in Cancer's Cause...

Characterisation of Antibodies with BRAND® PCR Plates

Introduction The generation of fully human antibodies with unique functional properties that are exploitable for tailored therapeutic interventions remains a major challenge in the antibody technology field. However, it is possible to isolate these antibodies from human B-cellls in contact with spec...

n-Butanol Molecular Biology Reagent

CAS RN 71-36-3 EC 200-751-6 Synonyms: 1-Butanol, Butyl Alcohol n-Butanol is useful for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation (Sambrook, p. 1.46). It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions...

Trending Papers

February 2017 Chimera animals have been created to both study drug effects in humans and also the goal of creating organs for use in humans. A recent breakthrough using CRISPR has occurred to knockout an animal fetus’ immune response to human cells. Now that the human cells can flourish in a non-hum...

Restriction Enzymes

Click here to return to the Molecular Biology Guide Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA. T...

Selecting Correctly Expressing Recombinants

• Blue White Screening • DNA Minipreps • Screening by Restriction Digestion • Screening by PCR • Confirm cut plasmid sizes by agarose gel electrophoresis • DNA Maxipreps • DNA Precipitation • RNase Treatment • Clean-up of DNA Blue / White colony screening is a strategy to quickly and easily distingu...

Horseradish (Armoracia rusticana) | Plant Profiler

Synonyms / Common Names / Related Links Allyl isothiocyanate, allylisothiocyanate, Armoracia lapathifolia Gilib., Armoracia rusticana, Armoracia rusticana Gaertner, Armoracia sativa Heller, Amoraciae Rusticanae Radix, Bohemian horseradish, Brassicaceae (family), Cochlearia armoracia, Cochlearia rust...

Long and Accurate PCR Amplification of DNA D8045 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1. effective denaturation of DNA template 2. adequate extension times to produce large products and 3. protection of target DNA from damage by depurination. Effective denaturation is accomplished by the use of higher temper...

KAPA Long Range PCR Kits FAQs

For which applications should I use KAPA Long Range PCR Kits? • PCR amplification of long targets and/or PCR using low amounts of template DNA. • Standard short- and mid-range PCR amplification. • Amplification of PCR products to be used for ligation into 3′-T-overhang cloning vectors. What is the e...

Carrot (Daucus carota) | Plant Profiler

Synonyms / Common Names / Related Terms Alpha-carotene, anthocyanins, beta-carotene, carotenoid, carotenoids, carrot cake, carrot jam, carrot juice, carrot puree, carrot soup, Daucus carota, dietary fiber, grated carrots, lycopene, lycopene red carrots, myristicin, purple carrots, red carrots, Umbel...

GenElute Bacterial Genomic DNA Kit Protocol

Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and purify genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in co...

Mixed Bed Resin Molecular Biology Reagent

CAS RN 100915-97-7 A self-indicating Mixed Bed Resin for deionizing formamide, acrylamide, glyoxal, PEG and urea. The beads change color from blue to gold when exchange capacity is reached. Room Temperature This product is for R&D use only, not for drug, household, or other uses. Please consult the ...

Clove (Syzygium aromaticum) | Plant Profiler

Synonyms / Common Names / Related Terms 2-methoxy-4-(2-propenyl)-phenol, Caryophylli atheroleum, Caryophylli Flos, caryophyllum, caryophyllus, Caryophyllus aromaticus, cengke, cengkeh, chiodo di garofano (Italian), choji, chor boghbojh, chor poghpch, cinnamon nails, clau, clavos, clou de girofle, Cl...

Industrial Microbiology Testing

Higher Quality with Microbiology Testing Beyond a complete range of high quality microbial testing solutions for the pharmaceutical, cosmetic, municipal water, beverage and food industries, we also provide regulatory expertise, comprehensive services, and trust, so you can achieve that one invaluabl...

Cancer Research: Epigenetic Mechanisms | BioFiles Volume 5 Number 6 - Cancer

BioFiles Volume 5, Number 6 — Cancer Download BioFiles v5 n6 (3.38 Mb PDF) Table of Contents • Introduction • New Cancer Biomarker Breast Cancer Malignant Melanoma Epithelial Ovarian Cancer Colon Cancer Summary • Cancer Research: Epigentic Mechanisms • Folic Acid Metabolism: A Role in Cancer's Cause...

Probiotics

The name “Probiotics” is derived from Latin (pro) and Greek (biotic) roots meaning “for life”. Probiotics are defined as live bacteria with beneficial effects to the health of the host organism. Today, primarily lactic acid bacteria (LAB) and bifidobacteria are used as probiotics, however certain ye...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

About the Sigma-Aldrich NAOSMM Program

Become the VIP of your Lab Members of the MilliporeSigma NAOSMM Program gain access to dedicated member discounts, freight savings and member only promotions. Check out some of our highlighted product areas below. Want to view your pricing? If you have already registered with MilliporeSigma to recei...

Cell Dissociation with Trypsin

Related Content • Trypsin • Trypsin Inhibitors • Antibiotics • Antibiotic selector tool • StableCell™ Trypsin Related Products • Cell Culture Media and Reagents • Growth Factors • ECM Protein • ECACC cell lines • Cell Freezing Media and Reagents • Fetal Bovine Serum (FBS) • Human Sera • Cell Dissoci...

Exploring Colon Cancer with Prestige Antibodies®

Colorectal cancer (CRC) is one of the most common types of cancer. Each year, approximately one million new cases are detected, and approximately 600,000 deaths can be attributed to this disease worldwide. Today, surgery is the only curative treatment for colorectal cancer, but adjuvant treatment ma...

Metabolomic Profiling of Neurospora crassa Fungi

Related Articles Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell Fused-Core HPLC Columns Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical Compounds Metabolites – A Serious Challenge for LC/MS Separations and Usual Detection Techniques Serum ...