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279 matches found for antibiotics

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein View PDF Version (246 Kb) Abstract Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has a...

Contact Plates for Personnel and Surface Monitoring

Our ready-to-use contact plates can be used for environmental monitoring of surfaces in critical clean rooms and isolators (ICR series) as well as in non-critical areas (RT series). To minimize the risk of contamination, our contact plates are produced in clean rooms. They can be stored at room temp...

Sigma Life Science Online Product Catalog

Biochemicals & Reagents • Amino Acids • Antibodies • Antibiotics • Biological Buffers • Carbohydrates • Detergents • Enzymes, Inhibitors, and Substrates • Life Science Labware • Lipids • Peptides • Proteins & Derivatives • Stable Isotopes • Additional Biochemicals & Reagents Techniques & Application...

Protocol Guide: MTT Assay for Cell Viability and Proliferation

Introduction The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT) to purple ...

What is Zinc Finger Nuclease (ZFN) Technology?

Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Zinc Finger Nucleases: Highly-specific Genomic Scissors Figure 1: Each Zinc Finger Nuclease (ZFN) consists o...

MISSIONTM CRISPRa Synergistic Activation Mediator (SAM)

What is CRISPRa and how can it complement your research? Loss-of-function (LOF) screening is a powerful approach to uncovering gene targets to understand the molecular mechanisms behind health and disease. However, screening the over-expression, or activation, of genes with CRISPR activators (CRISPR...

Method Validation for Determination of Chloramphenicol in Urine Using Molecularly Imprinted Polymer (MIP) SPE

• • Download PDF • Rejtharová Martina, Rejthar Libor rejthar@uskvbl.cz Institute for State Control of Veterinary Biologicals and Medicaments, Hudcova 56a, CZ 621 00 Brno, Czech Republic The method for determination of low concentrations of chloramphenicol in bovine and porcine urine was developed. S...

Protocol Guide: XTT Assay for Cell Viability and Proliferation

Product No. 11465015001 The non-radioactive, colorimetric assay system using XTT (sodium 3´-[1- (phenylaminocarbonyl)- 3,4- tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was first described by Scudiero, P.A. et al. 1, 2 and improved in subsequent years by several other investiga...

Drugs & Pharmaceuticals - Chromatography and Sample Prep Literature from Supelco

Pub. Id. Subject Type Size (KB) No. of Pages T196901 Solid Phase Microextraction/Capillary GC Analysis of Drugs, Alcohols, and Organic Solvents in Biolog Bulletin 901 116 8 T198922 SPME/GC for Forensic Applications: Explosives, Fire Debris, and Drugs of Abuse Bulletin 922 235 8 T199925 SPME Applicat...

dipeptidase 2
Synonyms MBD2
Species Human (64174) , Species Mouse (319446) , Species Rat (291984) , Species sheep (101110863) , Species domestic guinea pig (100726848) , Species naked mole-rat (101699075) , Species chicken (415714) , Species domestic cat (101091578) , Species cow (539370) , Species Horse (100066437) , Species dog (611083)
Summary: DPEP2 belongs to the membrane-bound dipeptidase (EC 3.4.13.19) family. These enzymes hydrolyze a variety of dipeptides, including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (Habib et al., 2003 [PubMed 12738806]

Blue-White Screening & Protocols for Colony Selection

Related Resources • Molecular Biology Handbook • Guide to E. coli markers Transformation • Introduction to Bacterial Transformation • Introduction to Yeast Transformation • Competent Cell Selection Guide • Transformation Protocols for Bacteria • Transformation Protocols for Yeast Return to the Molec...

Sterility Testing

Request More Information Sterility testing is an essential part of validation for products manufactured according to GMP purporting to be sterile. False negative results could lead to the release of contaminated products, whereas false positives could give rise to lengthy and costly investigations. ...

Analysis of Difficult Polar Compounds using Sigma-Aldrich Ion Pair Reagents and Supelco Ascentis HPLC Columns

Scientist Quality Assurance … htinner@europe.sial.com Because reversed-phase HPLC is primarily dependent on hydrophobic interactions between the stationary phase and analyte, ion pairing is occasionally necessary to obtain sufficient retention of polar, ionizable compounds. Although there is debate ...

Thiazoles and Imidazoles

• • Download PDF • Thiazoles and imidazoles have been frequently discovered as a vital component of novel and structurally diverse natural products that exhibit a wide variety of biological activities. The exceptional range of antitumor, antiviral, and antibiotic activities, as well as their presenc...

CONTROLLER Chemically Competent Cells

Sigma CONTROLLER Chemically Competent Cells are provided in 40 μL aliquots, sufficient for a single transformation. Transformation is performed by incubation on ice followed by heat shock at 42°C. For maximal transformation efficiency, the heat shock is performed in 15 mL polypropylene culture tubes...

Corning Cellgro Cross-Reference

Corning Cellgro, cell culture cross-reference chart for our cell culture products. Find direct replacements for Corning Cellgro's cell culture products. Stock up now on cell culture media and reagents We currently have large amounts of these products available for delivery today. You can depend on o...

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

Product No. CELLPRO-RO The measurement of cell proliferation and cell viability has become a key technique in the life sciences. The need for sensitive, reliable, fast and easy methods has led to the development of several standard assays. Proliferation assays have become available for analyzing the...

Formats — Glycerol FAQs

What should I do if I have trouble obtaining high yields of plasmid DNA? How should I store my bacterial cultures? What is the expected pDNA yield from glycerol stocks? I have several of the MISSION® shRNA clones in the bacterial stocks. I was wondering what the competent cells used are and if you h...

Mycoplasma Detection and Elimination

• Mycoplasma contamination is a major headache for the cell culturist. Mycoplasma are small, simple bacteria which lack a cell wall and represent one of the most prevalent and serious sources of cell line contamination. Mycoplasma contamination is invisible with no discernible change in turbidity or...

Settle Plates for Active and Passive Air Monitoring

Request More Information Related Links • View Product List Gamma-irradiated Settle Plates • View Product List Non-irradiated Settle Plates • Industrial Microbiology Testing Portfolio • On-demand Webinar: Environmental Monitoring in Aseptic Manufacturing • Pharmaceutical Microbiology Testing • Brochu...

EnPresso FAQs

Top Three Questions General topics E. coli cultures P. pastoris cultures Q. What are the most common reasons for lower than expected yield? • Use of incorrect closures leads to poor oxygenation (DO NOT USE aluminum foil, or plastic lids) • Overfilling of flask leads to poor oxygenation (maximum 10% ...

OverExpress™ Chemically Competent Cells

OverExpress™ Chemically Competent Cells are provided in aliquots of 40 μL sufficient for one transformation reaction. Transformation is performed by heat shock at 42°C, followed by incubation on ice. To ensure successful transformation results, the following precautions must be taken: • For best res...

PKH Bibliography

1. Akewanlop C, Watanabe M, Singh B. et al. Phagocytosis of breast cancer cells mediated by anti-MUC- monoclonal antibody, DF3, and its bispecific antibody. Cancer Res 2001 May; 61(10):4061-5. 2. lbertine KH, Gee MH. In vivo labeling of neutrophils using a fluorescent cell linker. J Leukoc Biol 1996...

Paints & Coatings Solvents Air Monitoring Applications

Solvents are one of the three components of paints. They are petroleum-based chemicals which dissolve the pigment and binding agent for application. Most enamel-based paints use a mild petroleum-based solvent with an alkyd vehicle, and have a long drying and curing time. Conversely, lacquer-based pa...

Ascentis Application Index by Compound Class

Listing by compound class Return to index selection HPLC Applications Application Report No. C18 RP-Amide C8 Aromatics 279 140 Artificial Sweeteners 266 Ascorbic Acid 193 Benzoic Acid Derivatives 270 146 Catechols and Resorcinols 141 Drugs of Abuse 336, 337, 338, 339, 340, 341, 342, 343, 344 Estroge...

Amberlite and Amberlyst Resins - Technical Information Bulletin

Home | Amberlite® and Amberlyst® Resins | Dowex® Resins | Sephadex® Products Merrifield Peptide Resins | Polyethylenimine-Based Products | Polyamide Resins A. Specialty Resins and Adsorbants 1. Amberlite Chromatographic Grade Ion-Exchange Resins 2. Amberlite Selective Ion-Exchange Resins 3. Amberlit...

Web Toolbox - Calculators, Search Explorers and Resources

Web Toolbox - Interactive Calculators, Search Explorers, and Resources The Web Toolbox features helpful online science research tools and resoures for Analytical Chemistry, Life Science, Chemical Synthesis and Materials Science. Structure Search Normality Molarity Calculator Pressure Temperature Nom...

Plant Tissue Culture Products | Agriculture

Our combination of quality products, services and global business capabilities help you to maintain reagent consistency while ensuring suitability. Sigma-Aldrich allows you to standardize reagents across multiple global locations by using a single lot of material. Our distribution capabilities and r...

Biotech Startup Essentials Program

GET STARTED NOW With the multitude of bioscience suppliers and business service options available, it can be a challenge just to figure out where to start. Our Biotechnology Essentials Starter Program simplifies the task of procuring all of the supplies and equipment your team needs to transform exp...

Organic TraceCERT Portfolio Keeps Growing! New Organic Certified Reference Materials

Analytix Reporter Issue 1 | 2018 Products & Related Products • View Product List • Organic CRMs for Chromatography (neats) • Organic CRMs • TraceCERT® CRMs for quantitative NMR (qNMR) Related Articles • Analytix Reporter Vol. 1 (PDF) • Caffeine, Taurine and Arginine in Shampoos by HPTLC • ±threo-Rit...

Ready-to-use validated zinc fingers for permanent knockout of your favorite gene

We offer ready-to-use, pre-constructed and functionally validated CompoZr Knockout Zinc Finger Nucleases (ZFNs) that are designed to permanently knockout any human, mouse or rat gene in a quick, simple and target specific manner. CompoZr Knockout ZFNs are a class of engineered DNA-binding proteins t...