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133 matches found for bacterial_conjugations

Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides

This protocol is for conjugating NHS-ester modifications, such as TxRd (Sulforhodamine 101-X) (protocol is effective for most NHS-ester modifications), to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6 (Figure 1). Since TxRd (Sulforhodamine 101-X) and several other modification...

Authenticated Colorectal Cancer Cell Lines for Cancer Research

Related Links • Cell Culture • ECACC Cell Lines • Protocols for ECACC Cell Lines • Browse Colorectal Cancer Cell Lines Related Articles • Exploring Colon Cancer with Prestige Antibodies® • MSI Testing and IHC • Colorectal Cancer and Immunohistochemistry • Breast Cancer Cell Lines • Liver Cancer Cell...

Precise Nanoparticles for Optoelectronics Applications

Benny Pacheco* and Scott Kordyban** Product and Business Development Manager Cytodiagnostics, Inc. 919 Fraser Dr, Unit 11 Burlington, ON L7L 4X8 *Email: bpacheco@cytodiagnostics.com **Not pictured Inorganic nanomaterials are tunable by size, shape, structure, and/or composition. Advances in the synt...

Chemistry at Surfaces with Self-Assembled Monolayers and SAMDI-ToF Mass Spectrometry

Prof. Milan Mrksich Department of Chemistry The University of Chicago Chicago, IL mmrksich@uchicago.edu Modern synthetic chemistry permits the construction of elaborate molecular structures and has been vital to the development of pharmaceuticals, catalysts, and functional polymers. Chemists no long...

Procedure for Conjugation of Alkaline Phosphatase to Antibodies and Other Proteins

Procedure for Conjugation of Alkaline Phosphatase to Antibodies and Other Proteins At least five different methods for conjugation of alkaline phosphatases to proteins have been described.1 The most popular method is the one-step glutaraldehyde coupling method.2 Procedure: 1. Combine 10 mg of antibo...

Extended Lifetime Ethanol Metabolites and Method Development for Ethyl Sulfate and Ethyl Glucuronide Separation

In this study methods are presented for ethanol metabolites ethyl glucuronide (EtG) and ethyl sulfate (EtS) Alcohol abuse remains an epidemic causing widespread health, legal, and economic impacts. Extreme abuse has escalated in volume and cases of alcohol abuse are consistently increasing in younge...

Free Radical Initiators

Photoinitiators | Thermal Initiators | Stabilizers Initiator/Stabilizer FAQ Dr. S.S. Newaz Polyorganix Inc., Houston, TX Q. How does one choose the appropriate initiator? In a free radical addition polymerization, the choice of polymerization initiator depends mainly on two factors: a) its solubilit...

Enzyme Explorer Drug Metabolism Tech Review

Multi-Drug Resistance in Cancer - Role of ABC Transporter Proteins Balázs Sarkadi1, Gergely Szakás1,2 and András Váradi3 1. National Medical Center, Institute of Hematology and Immunology, Budapest, Hungary 2. National Cancer Institute, NIH, Bethesda, MD, USA 3. Institute of Enzymology, Hungarian Ac...

Duolink® PLA Probemaker Guide

This guide describes the use of the Duolink® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink® PLA experiments. Probemaker-generated PLA antibodies can be used in combination with or independent from standard Duolink® PLA Probes, as lo...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Development of Small Molecule Donors for Solution-Processed Organic Solar Cells

Abby-Jo Payne and Gregory C. Welch Dalhousie University, Department of Chemistry 6274 Coburg Road, Halifax, Nova Scotia, Canada B3H 4R2 Email: gregory.welch@dal.ca Solution-processed organic photovoltaic devices (OPVs) have emerged as a promising clean energy generating technology due to their ease ...

FITC-labelled polysaccharides

FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

Partial PROTACs for Targeted Protein Degradation

An emerging strategy in drug discovery to access difficult-to-treat diseases is targeted protein degradation. While traditional small-molecule or antibody drugs may only allow access to ~20% of the proteome, degradation techniques may open the door to the other 80%.1 The molecules used in these appr...

NTz Building Blocks for Organic Semiconductors

Fax: +81-48-462-1473, E-mail: takimiya@riken.jp The field of organic electronics has emerged as the next-generation technology potentially enabling ultra-thin, large-area, and/or flexible devices, consisting of organic field-effect transistors (OFETs), organic light-emitting diodes (OLEDs), and orga...

Initiator/Stabilizer FAQs

• • Download PDF • Dr. S. S. Newaz Polyorganix Inc. Houston, TX Q. How does one choose an appropriate initiator? In a free-radical addition polymerization, the choice of polymerization initiator depends mainly on two factors: a) its solubility and b) its decomposition temperature. If the polymerizat...

Functional RAFT Polymers for Biomedical Applications

M. Shahinur Rahman, Sebastian Grajales MilliporeSigma, Milwaukee, WI Reversible addition–fragmentation chain transfer (RAFT) polymerization is rapidly moving to the forefront in construction of drug and gene delivery vehicles. The RAFT technique allows unprecedented latitude in the synthesis of wate...

Triazolylphenylglyoxal Reagents: Arginine-Directed Bioconjugation

Chemical ligation strategies are widely utilized for the modification of biological macromolecules in biotechnology and medicine and for the development of functional materials. In particular, reagents that can modify the surface of proteins, typically through reactive side chains, have found broad ...

Mono-Protected Diamino-PEG Spacers

• • Download PDF • Our highly purified mono-protected homobifunctional polyethyleneglycols display oligomer purities of more then 90%. This extremely narrow distribution of oligomers ensures homogeneous products in many applications such as conjugation reactions or introduction of solubilizing units...

Duolink® PLA Product Selection Guide

Getting started with Duolink® PLA is simple. The protocols are very similar to that of IF, IHC, and flow cytometry, however Duolink® PLA offers distinct advantages over these and other traditional methods of protein detection. The Duolink® PLA Starter Kit contains all the necessary reagents you need...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Atto Dyes for Superior Fluorescent Imaging

• Alternatives to common Fluorophores • Reactive Atto Dyes • Atto Dye Conjugates Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridiza...

Functional Biomaterials Synthesized by Double-Head Polymerization Agents

Hua Wei1 and Suzie H. Pun2* 1Department of Chemistry, Lanzhou University, Lanzhou 730000 China 2Department of Bioengineering and Molecular Engineering and Sciences Institute University of Washington, Seattle, Washington 98195 USA *Email: spun@u.washington.edu Over the past two decades, the rapid adv...

Polyethylene Glycol Building Blocks for PEGylation

Related Links • PEG Selection Guide • • Download PDF • Circulatory half-life is a key success factor for new drugs. In this respect, PEGylation or PEG-ing—the modification of potential candidates ranging from non-peptidic small molecules to peptides and proteins, antibody fragments, aptamers, and sa...

Non-Toxic ADC-Mimic for LC-MS Method Development

ADCs conjugated at Cys residues of inter-chain disulfide bonds give mAbs bearing 0-4 pairs of drug-linkers. Characterizing this distribution is paramount to understanding ADC efficacy and process control. Here we show how LC-MS and immunopurification methods may be optimized using a non-toxic surrog...

Antibody-Drug Conjugation [VIDEO]

Antibody-Drug Conjugation Video SAFC offers a comprehensive range of ADC services needed for development, manufacturing, and testing of bulk drug substance and drug product. Our latest offering to simplify your path to the patient is the opening of our state-of-the-art Commercial ADC manufacturing s...

Enzyme Explorer Product Application Index for Superoxide Dismutase

Superoxide Dismutase (SOD) catalyzes the reduction of superoxide anions to hydrogen peroxide. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion, which inactivates NO to form peroxynitrite. Therefore...

Clean-up of Antibody Labeling Reactions Using Amicon® Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample. Use the protocol below to remove unreacted fluorescent labels using centrifugal ultrafiltration devices. Remova...

Superior Fluorescent Imaging Using Atto Dyes

• • Download PDF • Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, and enzyme assays. The A...

Duolink® PLA Multicolor Detection Protocol

Related Products • Duolink® PLA • Duolink® PLA Probemaker Guide for Multicolor Detection • PLA-Validated Primary Antibodies • Duolink® PLA Custom Services Related Articles • How to optimize the Duolink® Proximity Ligation Assay • Duolink® PLA Trouble Shooting Guide • How Proximity Ligation Assay (PL...

Chitosan Biopolymer from Fungal Fermentation for Delivery of Chemotherapeutic Agents

David Brown,1 Keith Brunt,2 Nils Rehmann3 1CTO Mycodev Group, Fredericton, NB E3B 6B3 Canada 2Dalhousie School of Medicine New Brunswick, Saint John, NB E2K 5E2, Canada 3NiRem Consulting, 8 Fish Hatchery Lane, French Village, NB E3E 2H5, Canada Email: Dave@mycodevgroup.com,1 Nils@nirem.ca3 Chitosan ...