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134 matches found for bacterial_conjugations

Model Systems for Metabolomics and Drug Toxicity Studies

Robert Gates Robert Gates Product Manager Email: robert.gates@sial.com Over the past three decades non-cellular and cellular systems have supplanted whole animal studies for the preliminary screening of drug candidates and well as for initial drug metabolism and toxicology studies. In 1991 inadequat...

Secondary Antibodies, Conjugates and Kits

Related Links • Antibody Explorer • How to Choose a Secondary Antibody Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purifi...

"Click" Chemistry in (Bio)Materials Science

• • Download PDF • 1Organic Chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, The Netherlands 2Encapson B.V, The Netherlands j.vanhest@science.ru.nl Combining biomolecules and synthetic polymers into a new class of versatile biohybrid materials with many (future) fields ...

Graphene in Biotechnology

Alba Centeno and Amaia Zurutuza Elorza* Graphenea S.A. Tolosa Hiribidea, 76, E-20018 Donostia - San Sebastian, Spain *Email: a.zurutuza@graphenea.com Graphene has emerged as the new wonder material. Being only one atom thick and composed of carbon atoms arranged in a hexagonal honeycomb lattice stru...

NIPAM based Polymers

Gangadhar Panambur, Ilya Koltover and Scott Batcheller MilliporeSigma, 6000 N. Teutonia Milwaukee, WI 53209 Stimuli-responsive Polymers Stimuli-responsive polymers, also known as “smart” polymers or “environmentally sensitive” polymers, undergo rapid changes in their microstructure from a hydrophili...

Chemistry at Surfaces with Self-Assembled Monolayers and SAMDI-ToF Mass Spectrometry

Prof. Milan Mrksich Department of Chemistry The University of Chicago Chicago, IL mmrksich@uchicago.edu Modern synthetic chemistry permits the construction of elaborate molecular structures and has been vital to the development of pharmaceuticals, catalysts, and functional polymers. Chemists no long...

Properties and Applications of Functionalized Graphene Oxide

1Claremont University, Keck Graduate Institute, School of Applied Life Sciences Claremont, CA 91711, USA 2Keck Science Department, Claremont McKenna College, Claremont, CA, 91711, USA 3Keck Science Department, Pitzer College, Claremont, CA, 91711, USA *E-mail: Kiana_Aran@kgi.edu Article Sections • I...

Light-Emitting Polymers

Related eTool • OLED eFabricator • • Download PDF • Department of Materials Science and Engineering, California NanoSystems Institute, and Henry Samuli School of Engineering and Applied Science, UCLA Conjugated polymers with long range p-electron delocalization behave as processable organic “metals”...

Safe Manufacture of Antibody Drug Conjugates

Although antibody-drug conjugates show promise in the development of effective cancer therapies, there are still a variety of challenges involved in enabling the safe manufacture of these hazardous drugs. Antibody-drug conjugates (ADCs) – anti-cancer drugs which combine the specificity of a monoclon...

Partial PROTACs for Targeted Protein Degradation

An emerging strategy in drug discovery to access difficult-to-treat diseases is targeted protein degradation. While traditional small-molecule or antibody drugs may only allow access to ~20% of the proteome, degradation techniques may open the door to the other 80%.1 The molecules used in these appr...

Duolink® PLA Probemaker Guide

This guide describes the use of the Duolink® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink® PLA experiments. Probemaker-generated PLA antibodies can be used in combination with or independent from standard Duolink® PLA Probes, as lo...

Silica-coated Gold Nanoparticles: Surface Chemistry, Properties, Benefits and Applications

In the past few decades, research and commercial use of gold nanoparticles has rapidly expanded into fields extending from biomedical engineering1 to photovoltaics.2 Most applications take advantage of the excellent optical properties of gold nanoparticles, which can be fine-tuned by varying their s...

Development and Manufacturing Highly Potent APIs

SAFC’S leading position in the fast-growing Highly Potent Active Pharmaceutical Ingredients (HPAPI) market includes conjugation of HPAPIs to antibodies for exciting new anti-cancer drugs. HPAPIs are proven to be effective at much smaller dosage levels than traditional APIs, but their potent characte...

Create Your Own PLA® Probes

Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue. Direct detection and localization of protein interactions are achieved with unparalleled specifi...

NTz Building Blocks for Organic Semiconductors

Fax: +81-48-462-1473, E-mail: takimiya@riken.jp The field of organic electronics has emerged as the next-generation technology potentially enabling ultra-thin, large-area, and/or flexible devices, consisting of organic field-effect transistors (OFETs), organic light-emitting diodes (OLEDs), and orga...

Organic Bioelectronic Materials and Devices for Bridging Biology and Traditional Electronics

1 Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA 2 Simpson Querrey Institute for BioNanotechnology, Northwestern University, Chicago, IL 60611, USA 3 Department of Chemistry, Imperial College London, London SW7 2AZ, UK 4 Department of Physics and Center for Pl...

Competent Cell Selection & E. coli Markers Guide

Transformation • Introduction to Bacterial Transformation • Introduction to Yeast Transformation • Introduction to Blue-white Screening • Transformation Protocols for Bacteria • Transformation Protocols for Yeast Related Resources • Molecular Biology Handbook • E. coli genotypes • Cloning & Expressi...

FITC-labelled polysaccharides

FITC-Dextran FITC-DEAE-dextran (FDD) FITC-carboxymethyl-dextran (FCM-Dextran) FITC-polysucrose (FITC-Ficoll®) FITC-DEAE-polysucrose FITC-CM-polysucrose (FITC-CM-Ficoll®) FITC-inulin Fluorescein hyaluronic acid (FHA-Se) TRITC-labelled polysaccharides TRITC-polysucrose (TRITC-Ficoll®) Tetramethyl-rhod...

Functionalized Pei and Its Role in Gene Therapy

1Department of Pharmacy, Pharmaceutical Technology and Biopharmacy, Ludwig-Maximilians-Universität München, Butenandtstraße 5, 81377 München, Germany 2Nanosystems Initiative Munich (NIM), Ludwig-Maximilians-Universität München, Schellingstraße 4, 80799 München, Germany *Email: Olivia.merkel@lmu.de G...

Drug Conjugate Analysis

• • Download PDF • Glucuronidation by the human UDPglucuronosyltransferase (UGT) family of enzymes plays an important role in the metabolic fate of many drugs and other xenobiotics. This biosynthetic reaction also has a role in the conjugation and excretion of endogenous substrates, such as steroids...

Non-Toxic ADC-Mimic for LC-MS Method Development

ADCs conjugated at Cys residues of inter-chain disulfide bonds give mAbs bearing 0-4 pairs of drug-linkers. Characterizing this distribution is paramount to understanding ADC efficacy and process control. Here we show how LC-MS and immunopurification methods may be optimized using a non-toxic surrog...

Trifunctional Probe Building Blocks

Related Articles • Chemical Synthesis Technology Spotlights Chemical probes that mimic a pharmacophore, ligand, or lead compound are of increasing interest for target identification and validation, protein profiling, imaging, or other applications in chemical biology. However, synthesis of the ideal...

Fundamental Evaluation of the Stationary Phase Retention and Selectivity on the Ascentis®Express Biphenyl

While C18 alkyl-based phases remain the gold standard for reversed-phase liquid chromatography, stationary phases that invoke interactions in addition to hydrophobic partitioning afford the method developer a viable option for improving resolution without sacrificing robustness or reproducibility. P...

MALDI-TOF Characterization of Functionalized Polymers

With an increased demand for high performance and specialized polymeric materials, tools for selective end group functionalization and modification have been a focus of synthetic development. Due to their tunable physical and chemical properties, polymers are an essential component for a variety of ...

Enzyme Explorer Drug Metabolism Tech Review

Multi-Drug Resistance in Cancer - Role of ABC Transporter Proteins Balázs Sarkadi1, Gergely Szakás1,2 and András Váradi3 1. National Medical Center, Institute of Hematology and Immunology, Budapest, Hungary 2. National Cancer Institute, NIH, Bethesda, MD, USA 3. Institute of Enzymology, Hungarian Ac...

Autophagy and Mitophagy in Tumor Cell Survival and Death

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them. In mammalian cells, autophagy occurs constitutively at basal rates and its primary function is to...

Designing Custom Peptides

While peptide synthesis can be straightforward, it is important to consider several factors before synthesis commences. The sequence, amino acid composition and length of a peptide will influence whether correct assembly and purification are feasible. These factors also determine the solubility of t...

Duolink® PLA Product Selection Guide

Getting started with Duolink® PLA is simple. The protocols are very similar to that of IF, IHC, and flow cytometry, however Duolink® PLA offers distinct advantages over these and other traditional methods of protein detection. The Duolink® PLA Starter Kit contains all the necessary reagents you need...

Functional Biomaterials Synthesized by Double-Head Polymerization Agents

Hua Wei1 and Suzie H. Pun2* 1Department of Chemistry, Lanzhou University, Lanzhou 730000 China 2Department of Bioengineering and Molecular Engineering and Sciences Institute University of Washington, Seattle, Washington 98195 USA *Email: spun@u.washington.edu Over the past two decades, the rapid adv...

Functionalized Polyethylene Glycol (PEG) Reagents

Related Links • PEG Selection Guide The chemical modification of biologically active compounds, such as peptides, proteins, antibody fragments, aptamers, enzymes, and small molecules with polyethylene glycol (referred to as “PEGylation”), is an effective method to tailor molecular properties to part...