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51 matches found for cell_attachment

A Dynamic Live Cell Assay Platform

A Dynamic Live Cell Assay Platform Autophagy (self-eating) is a complex cellular process essential for cell survival under stressed conditions. Recent evidence has further suggested that tumor cells use the autophagic pathway to promote survival under stressed conditions, such as nutrient deprivatio...

Media Expert® - cell culture media components

The Media Expert (ME®) is the Web's most comprehensive source of information on cell culture media components and formulation: • Provides reviews on all major components used in cell culture media • From albumin to zinc • Provides information on formulation strategies • Primary functions for each co...

Basal Salts for Cell Culture

Dulbecco's Phosphate Buffered Saline Earle's Balanced Salts Hanks' Balanced Salts Tyrode's Salts Other Salt Mixtures Families of basal salt solutions are often named for their physician or scientist inventor. In 1885, Sydney Ringer developed a solution of inorganic salts designed to maintain contrac...

Attachment-Dependent Cell Growth and Cell Proliferation in the MultiScreen Assay System

Year: 1995 Numerous bioassays utilize cell cultures to quantitate the effects of potential growth modifiers. These assays are typically performed in 96-well microwell plates for “convenience” and are usually followed by tedious cell harvesting. The MultiScreen assay system, however, offers an altern...

Establishing a time course for translocation of FOXO4 in live cells using a novel microfluidic culture platform assay

Cindy SY Chen, Fen Xu, Terry Gaige and Paul J. Hung | MilliporeSigma Introduction Forkhead box O (FOXO) belongs to the large Forkhead transcription factor family that were first found to be altered in several types of cancers such as alveolar rhabdomyosarcomas and mixed-lineage leukemias1,2. These t...

Chitosan-based Biomaterials

Chitin, a natural polysaccharide, is the second most abundant natural biopolymer in the world, after cellulose. It is the primary, high modulus fibrous component of the exoskeleton of arthropods including crab, shrimp and lobsters, as well as some fungi. Structurally, chitin is an insoluble, linear,...

Cell Dissociation with Trypsin

Related Content • Trypsin • Trypsin Inhibitors • Antibiotics • Antibiotic selector tool • StableCell™ Trypsin Related Products • Cell Culture Media and Reagents • Growth Factors • ECM Protein • ECACC cell lines • Cell Freezing Media and Reagents • Fetal Bovine Serum (FBS) • Human Sera • Cell Dissoci...

3D Bioprinting: Bioink Selection Guide

3D bioprinting enables the generation of precisely controlled 3D cell models and tissue constructs, by engineering anatomically-shaped substrates with tissue-like complexity. Due to the high degree of control on structure and composition, 3D bioprinting has the potential to solve many critical unmet...

Premium Tissue Culture Treated Plasticware

Our new line of cultureware meets the superior quality standards of premium tissue culture treated plasticware you’ve grown accustomed to, but is differentiated by our commitment to keep your work flowing seamlessly. We can provide you with a complete range of cell culture reagents, media, sera, cul...

Common Cell Culture Problems: Poor Attachment of Adherent Cells

Optimal cell attachment in vitro requires the interaction of healthy cells with a wide range of cell-derived attachment molecules present in media, serum, and supplements. Cultureware surfaces are frequently treated with extracellular matrix proteins such as collagen, fibronectin, laminin, or other ...

BRANDplates® cellGrade™ Plus Surface Minimizes Loss of Transfected Cells During Luminescence Assays

Related Products • Labware Cell attachment is considered to be critical for many cell culture applications since it triggers for instance differentiation, metabolic activity and gene expression (Chang et al. 2012). Medium changes and washing steps especially in automated culture processing or high c...

High-throughput Imaging of a Unique Continuous Flow Microfluidics Plate

The continued drive towards cell based assays that better mimic the in vivo environment has led to innovative cell culture systems such as the SciFlow™ 1000 Fluidic Culture System, which utilizes capillary channels to enable physiologically relevant fluid flow across cultured cells. In this applicat...

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the cell culture vessel, have proven to be efficient methods to better mimic in vivo cell behavior. We de...

HyStem™ Glossary of Terms

A | B | C | D | E | F | G | H | I | L | M | N | O | P | R | S | T | U A Acinus (plural: acini) - The word comes from the latin word “grape”. It refers to the sac-like termination of a secretary exocrine gland. In vitro, mammary epithelial cells cultured in a laminin-rich matrix form acinus-like sphe...

An ECM Mimetic Library for Engineering Surfaces to Direct Cell Surface Receptor Binding Specificity and Signaling

Sigma Product Listings The extracellular (ECM) microenvironment, defined by biochemical cues and physical cues, is a deciding factor in a wide range of cellular processes including cell adhesion, proliferation, differentiation, and expression of phenotype specific functions. For this reason, enginee...

Viral Invaders

Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing A Contamination-Free Future A New Line of Defense Lightening the (Viral) Load What is ZFN Technology? Choosing CHO An Ongoing Battle Scanning the Horizon Great progress has been made in t...

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Introduction HyStem™ Features and Benefits Protocols and Support Publications using HyStem™ with Applications Examples of Cells That Have Been Cultivated Successfully with HyStem™ Platform HyStem™ is a semi-synthetic 3D hydrogel platform that allows cell growth in a 3D environment. Their main compon...

Bioprinting for Tissue Engineering and Regenerative Medicine

Chi-Chun Pan,1,2 Arnaud Bruyas,1 Yunzhi Peter Yang1,3,4 Departments of 1Orthopedic Surgery, 2Mechanical Engineering, 3Materials Science and Engineering, and 4Bioengineering Stanford University, 300 Pasteur Drive, Stanford, CA 94305 Email: ypyang@stanford.edu In the past two decades, tissue engineeri...

Cell Culture Fundamentals: Alternative High-Yield Cell Culture Systems

Most tissue culture is performed on a small scale where relatively small numbers of cells are required for experiments. At this scale cells are usually grown in T-flasks ranging from 25cm2 to 175cm2. Typical cell yields from a T175 flask range from 1x107 for an attached line to 1x108 for a suspensio...

The BRAND® Insert 2in1 Cell Culture Inserts

Overview • Suitable for 2D and 3D cell cultures • Surface: cellGrade™ plus • Membrane: PC or PET • Pore sizes: 0.4 µm and 8.0 µm • Can be used with all common 6-, 12- or 24-well multiwell plates Flexible Keep using the multiwell plates or dishes you already use in your cell culture lab – no change o...

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular material organized in a highly specialized manner. It is difficult to mimic all aspects of human biology wit...

Cell Culture Contamination

Cell Culture Contamination Download BioFiles v7 n1 (2.55 Mb PDF) Table of Contents • Antibiotics • Mycoplasma Detection/Elimination • Cell Culture Equipment Introduction Don Finley Life science research using cell cultures has resulted in numerous improvements to the treatment of human disease. In a...

High yields of transfected cells with BRANDplates® cellGrade™ premium surface

Transfection is defined as non-viral DNA/ gene delivery into eukaryotic cells performed by several chemical, physical or biological methods. The subsequent exogenous expression of a tagged protein in cell culture is a well established approach to investigate function and localization of the protein ...

Media Formulation Products

Carrier and Transport Proteins Hormones Hydrolysates Lipids and Lipid Carriers Other Supplements Carrier and Transport Proteins Cell culture is essential to biotechnology. Historically, cells have been cultured in serum containing media. Serum is a source of many nutrients required by cells to proli...

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

Product Number 3447-020-01 3-D Culture is an innovative approach to modeling the morphological effects of early oncogenesis on three-dimensional microenvironments. When healthy, differentiating cells exhibit a structured, polarized morphology that is critical for cellular formation and function. Dur...

Coating Tissue Culture Plates with Vitronectin

Product No. SRP3186 Vitronectin is used to coat culture vessels for cells attachment. Below is a suggested coating protocol for a 96-well plate, at a coating concentrations of 0.5 µg/cm2. Note that the optimal concentration vary with each cell line. For culture vessels other than 96-well, this proto...

ECM Coating Protocols for Millicell

Coating of membranes and plastic surfaces with extra cellular matrices (ECMs) promotes cell attachment and monolayer formation. We have developed protocols for four types of ECMs on Millicell®-CM inserts. They are also useful for growing cells on plastic feeder trays in co-culture experiments. Mater...

Fouling Resistant Biomimetic Poly(Ethylene Glycol) Based Grafted Polymer Coatings

• • Download PDF • Biomedical Engineering Department Northwestern University Evanston, IL philm@northwestern.edu Accumulation of biological matter at surfaces is an inevitable event in virtually any environment in which natural and man-made materials are used. Although sometimes fouling of surfaces ...

What is a Cell Delivery Vehicle?

Related Links • 3D cell culture • 3D hydrogels • HyStem® hydrogel Related Products • Cell culture • 3D cell culture • Definition • Purpose • Types of Vehicles • Vehicle Comparison • HyStem® Hydrogel Advantage • Materials • References A cell delivery vehicle is a matrix (made from natural or syntheti...

SciFlow™ 1000 Fluidic Culture System

Protocols • Coating of Well Surfaces Pre-Coat of a Thin Protein Layer Monolayer Culture • Fluorescein Standard Curve Calibration for Flow Tracking Drug Exposure & Monolayer Culture • Multiple Cell Types Adherent Cells Monolayer Culture • One Cell Type Adherent Cells Monolayer Culture Related Product...