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832 matches found for cell_culture

Why We Use Cell Lines as Models Webinar [VIDEO]

Why We Use Cell Lines as Models A 30 Year Journey and What Lies Ahead Webinar This webinar will discuss the evolving role of Cell Lines and Cell Culture and their usefulness as models of physiological function and disease. The webinar will review the depth, breadth and best use of currently availabl...

Roche Products and Biochemical Reagents

Whether working within a competitive funding landscape or tasked with hitting key business milestones towards financial deliverables, research scientists are also presented with more scientific information and challenges than ever before. As a result, researchers require reliable reagents that are a...

Cell Death Detection ELISA PLUS Protocol

Product No. CELLDETH-RO Use with non-proliferating cells Since the Cell Death Detection ELISAPLUS assay does not require prelabeling of cells, it can detect internucleosomal degradation of genomic DNA during apoptosis even in cells that do not proliferate in vitro (for example, freshly isolated tumo...

Structural Proteins

Structural proteins are the most abundant class of proteins in nature. Collagen is recognized as the most abundant mammalian protein. Structural proteins such as collagen, fibronectin and laminin are utilized in cell culture applications as attachment factors. We offer the most comprehensive collect...

Small Molecules Drug Development

When analyzing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. Our tools support the requisite techniques, such as gas chro...

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

MaxGel™ ECM is an in vitro derived human basement membrane extracts (BME) that provides a rich three-dimensional environment to promote cellular proliferation for multiple cell types. MaxGel™ ECM contains extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin, a...

Cell Proliferation ELISA, BrdU (colorimetric) Protocol & Troubleshooting

Product No. 11647229001 Measurement at different wavelengths It is possible to measure the color development over a period of time without adding stop solution and measuring at 370/492 nm, to easily identify the optimal experimental conditions. With addition of stop solution and measurement at 450/6...

Small Molecule Workflow

When analysing small molecules the overarching need is to maximize sensitivity without compromising reproducibility and laboratory throughput. We have developed unique tools to maximize speed and selectivity throughout the entire workflow. To support the diverse techniques required such as GC, spect...

Sigma-Aldrich Product Portfolio

Sigma-Aldrich gives you easy access to the product you need • by streamlining our product range • by delivering clearly defined product categories • by providing brands and grades for every application Sigma-Aldrich Brands PRODUCT BRANDS Supelco® Sigma® Sigma-Aldrich® SAFC APPLICATIONS Analytical Ca...

Sigma Life Science Online Product Catalog

Biochemicals & Reagents • Amino Acids • Antibodies • Antibiotics • Biological Buffers • Carbohydrates • Detergents • Enzymes, Inhibitors, and Substrates • Life Science Labware • Lipids • Peptides • Proteins & Derivatives • Stable Isotopes • Additional Biochemicals & Reagents Techniques & Application...

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the ...

Dry Powder Media Global Supply

SAFC Overview Why Choose SAFC? Media Supply Strategy DPM Manufacturing Capability and Capacity Packaging and Logistics Global Quality Systems Raw Materials Scale-up & Support Services Small Volume Custom Media – imMEDIAte Advantage™ Erythropoietin Hormone (Molecular model) Erythropoietin (EPO) is a ...

Targeted Gene RNAi Knockdown Experiments Measured Using AQUA

Richard J. Mehigh, Jeffrey J. Porter, Beth K. Ewing, Justin Wildsmith, Kenneth E. Heuermann, Kelly L. Foster, John G. Dapron, Steven L. Cockrill, William K. Kappel, and Graham B. I. Scott Download pdf version. (1.96 mb PDF) Abstract Introduction Materials Methods Results Conclusions References Abstr...

Webinar: Safety First Throughout the Histology Workflow

From tissue processing to slide coverslipping, the histology workflow is laden with hazardous steps. Chemical sensitizers, strong acids, alkaline substances, and oxidizing agents are routinely used during histological workflows. These classes of chemicals are known to damage and even destroy living ...

Filtration in Dissolution Testing: Improving Throughput and Reducing Variability

In vitro dissolution testing is used to characterize drug compounds throughout their development. In early drug development it is used to support the choice of a particular formulation. During drug production it is a critical component of the quality control process and is used to assess the changes...

MISSION® R&D Production Facility

Sigma-Aldrich is proud to provide innovative solutions for academic research and pharmaceutical drug discovery. Our excellent systems, equipment and personnel provide the high quality tools you require. The dedicated MISSION™ RNAi R&D and manufacturing facilities are housed in the Sigma-Aldrich Life...

Genotyping by Oligonucleotide Ligation Assay (OLA)

Product lists for commonly used protocols from Cold Spring Harbor Protocols Genotyping by Oligonucleotide Ligation Assay (OLA) Stuart J. Macdonald Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 67705, USA Department of Molecular Biosciences, University of Kans...

Cell line Authentication Test: STR profile

Cell culture underpins research throughout the life sciences. Continuous cell lines have been used as culture models for many cell types, and assembled into panels for drug discovery and development. However, problems like cross-contamination are quite common and can render some cell lines unsuitabl...

Serum-Free Stem Cell Media & Supplements

Historically, fetal bovine serum (FBS) has been the supplement of choice for in vitro mammalian cell culture including stem cells. However, due to the undefined composition of FBS, researchers have transitioned to more chemically defined serum-free and xeno-free media and supplements for stem cell r...

Cell Signaling & Neuroscience Assay Kits by Name

A-B C D-G H-L M-O P R-T Product Code NAME Area of Interest Platform Size Sensitivity Species Description CS0740 Acid Phosphatase Assay Kit Nitric Oxide/ Cell Stress Enzymatic 1,000 in microwell plate 0.2 mUnit mammalian Quantitative Acid Phosphatase activity measurement in tissues, whole cell extrac...

Cell Growth to Confluence [VIDEO]

Cell Growth to Confluence Return to Cell Culture Videos homepage

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) belongs to the interleukin-6 cytokine family. LIF binds to a heterodimeric receptor consisting of the low-affinity LIF receptor and gp130, with downstream signals being transmitted through gp130. There are a number of signaling pathways downstream of gp130, including...

Hematopoietic Stem and Immune Cell Culture

• Hematopoietic Stem Cells • Human Monocytes • Human Mononuclear Cells (PBMCs) • Human Macrophages • Human Dendritic Cells • Stromal Cells for T-Cell Differentiation • Stem Cell Protocols Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myelo...

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Related Products • Mesenchymal Stem Cell Culture • Stem Cell Culture • Stem Cell Protocols • Mobius Single Use Bioreactors • 3D Cell Culture • Primary Cell Culture • ECACC Cancer Cell Lines • Cell Culture Media and Reagents • Stericup® Media Filtration • Growth Factors • ECM Protein • Antibodies • B...

High-throughput Imaging of a Unique Continuous Flow Microfluidics Plate

The continued drive towards cell based assays that better mimic the in vivo environment has led to innovative cell culture systems such as the SciFlow™ 1000 Fluidic Culture System, which utilizes capillary channels to enable physiologically relevant fluid flow across cultured cells. In this applicat...

Preventing Culture Shocks: Understanding the Issues of Cell Line Authenticitycell-culture-webinar

Understanding the Issues of Cell Line Authenticity & How to Avoid Invalidating your Research Speaker: Jim Cooper, Cell Biology Applications Scientist, ECACC Date and time: 20th March 2014, 14:00 GMT / 15:00 CET / 09:00 CDT Cell culture underpins basic life science research, translational research, d...

siRNA Frequently Asked Questions for Functional Genomics and RNAi

If you do not find the answer here in our FAQ (frequently asked questions), please send a request to sirnarequest@sial.com. General Duplex Design Experiment & Analysis in vivo Applications & N-TER Use Validation & Guarantee What is siRNA? siRNA (short [or small] interfering RNA) is a class of small ...

Human iPS Cell Dissociation Reagents

Related Links • Stem Cell Culture • iPS Cell Culture Protocols • CRISPR Editing of Human iPSCs • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • RNA Reprogramming Kits • Human iPSC Media • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media Filtration • Growth Facto...

BRANDplates® cellGrade™ Plus Surface Minimizes Loss of Transfected Cells During Luminescence Assays

Related Products • Labware Cell attachment is considered to be critical for many cell culture applications since it triggers for instance differentiation, metabolic activity and gene expression (Chang et al. 2012). Medium changes and washing steps especially in automated culture processing or high c...

3D Cell Culture Tools and Applications

Cultivate reality with advanced 3D cell culture models. 3D cell culture techniques have led to the creation of more predictive in vitro cell models for numerous applications including cancer research, drug discovery, neuroscience and regenerative medicine. Cells naturally grow and differentiate in t...