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360 matches found for centrifugation

Protein Concentration for Large Volumes Samples

Ultrafiltration (UF) is a pressure-driven, convective process that uses semipermeable membranes to separate species by molecular size and shape. UF is highly efficient, allowing for concentration and desalting at the same time. Unlike chemical precipitation (such as with ethanol or phenol/chloroform...

Preparation of Plasmid DNA Protocol

Product lists for commonly used protocols from Cold Spring Harbor Protocols Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell. Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation Joseph Sambrook Peter Maccallum Cancer...

In Pursuit of the Elements

Brefeldin A from Penicillium brefeldianum

CAS RN 20350-15-6 Sysnonyms: Decumbin; Ascotoxin; Cyanein; BFA; g,4-Dihydroxy-2-(6-hydroxy-1-heptenyl)-4 cyclopentanecrotonic acid l-lactone Molecular formula: C16H24O4 Molecular weight: 280.36 Brefeldin A (BFA) is a fungal metabolite produced by Penicillium brefeldianum. It is known to inhibit prot...

Evaluation of Three Media for Capture of Recombinant Human Serum Albumin from Pichia pastoris and Scale-up Using Capto MMC

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013 Three media (Capto MMC, SP Sepharose Fast Flow, and SP Sepharose XL) were evaluated for their ability to capture recombinant human serum albumin (rHSA, pI 5.5) from cell culture supernatant (CCS) of Pichia pastoris. The CCS was clar...

Collagen Attachment Protocols, Solubility, and Stability

• • Download PDF • Collagen Type I (Cat. Nos. C1809, C7661, C9791, C8919*) Optimal conditions for attachment must be determined for each cell line and application. 1. Add collagen to 0.1 M acetic acid to obtain 0.1% (w/v) collagen solution. Allow to stir at room temperature 1-3 hours until dissolved...

Vermont Genetics Network Microarray Programs use Pellet Paint® Co-Precipitant

An important mission for the Vermont Genetics Network (VGN) is to identify needs and develop facilities for biomedical research at the University of Vermont (UVM, Universitas Viridis Montis, Latin for “University of the Green Mountains”). Additional network goals are to: (1) assist UVM faculty resea...

DNA & RNA Purification GenElute Agarose Spin Columns

Description: GenElute™ Agarose Spin Columns isolate DNA by centrifugation and filtration. Procedure: A slice of agarose gel containing the DNA of interest is excised, placed into a spin column, and spun at maximum speed in a microcentrifuge. Agarose is retained on a filter in the spin column. Solubl...

WGA4 - GenomePlex® Single Cell Whole Genome Amplification Kit

Product Description Reagents Provided Storage/Stability Procedure References Troubleshooting Guide FAQs Posters and Publications Downstream applications Gel Electrophoresis & qPCR CGH Microarrays Short Tandem Repeats Analysis Single Nucleotide Ploymorphism (SNP) Genotypying Data Whole genome amplifi...

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

There are a variety of methods for cellular disintegration and extraction of proteins from E. coli ranging from enzymatic digestion and osmotic shock to ultrasonication and pressure disruption. Each method has inherent advantages and disadvantages. Generally, vigorous mechanical treatments reduce vi...

EX-CELL® Advanced™ CHO Fed-batch Medium

Without L-glutamine, without hypoxanthine, and without thymidine Liquid Catalog No. 14366C Powder Catalog No. 24366C EX-CELL® Advanced™ CHO Fed-Batch Medium is a chemically defined, next generation media platform. The formulation was developed using multivariate analysis of 10,000+ data points that ...

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

Related Articles • Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid • Troubleshooting Analyte Recovery when Using HybridSPE-Precipitation Technology • Minimizing Phospholipid Matrix Effects using HybridSPE-PL • Phospholipid Removal Using HybridSPE™ Increases Assay Speed Phospholipids ...

HTS and Process Development for Capture of Recombinant Pro-insulin from E. coli using Capto MMC

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013 This study describes the development of a robust capture method for recombinant pro-insulin. PreDictor plates and Assist software were used to determine a chromatographic medium for capture and identify promising binding and elution...

Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit

A general protocol for purification and concentration of DNA from PCR mixtures, restriction enzyme digests, and solutions using illustra™ GFX™ 96 PCR Purification Kit is provided below. The kit can be used with suitable centrifuges, vacuum manifolds, or automated robotic workstations. Buffers and GF...

DNase I Recombinant, RNase-free Protocol

Product No. 04716728001 REMOVAL OF DNA FROM RT-PCR REACTIONS: Overview DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation. DNase I is a DNA-specific endonuclease that hydrolyzes ds or ssDNA to a mixture of oligo and mon...

Further Applications of Ficoll-Paque PLUS and Ficoll-Paque PREMIUM

Extracted from Isolation of Mononuclear Cells, Methodology and applications (PDF) GE Healthcare, 2010 A great many modifications and extensions of the method have come into use following the introduction of the technique described by Bøyum in 1968 and its subsequent widespread adoption. For example,...

EZview™ Red - High Visibility Affinity Gel

Technical Poster Data Ordering Information A major disadvantage of affinity-based molecular pull-down and immunoprecipitation procedures is that the affinity matrix is difficult to see in the microcentrifuge tube following centrifugation steps. To facilitate speed and accuracy, we have developed a d...

User Guide: MultiScreen® Solvinert Filter Plates

The MultiScreen® Solvinert Filter Plate is a single-use, automation-compatible 96-well device with chemically-resistant 0.45 µm PTFE membrane providing high flow for use in the processing of a wide range of aqueous and organic solutions. It is designed for use with vacuum or centrifugal pressure, ac...

Development of C18 SPME Fiber Probes for LC Applications

Related Articles • Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples • Accelerated Bioanalytical LC-MS-MS Using Ascentis® Express Fused-Core® HPLC Columns • Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column • Bioanalysis with S...

GenomePlex Whole Genome Amplification Kit Protocol

Product Description Components Storage/Stability Procedure Troubleshooting Guide Frequently Asked Questions Precautions and Disclaimer Materials References GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, ~500-fold amplification of geno...

Cell disruption and membrane preparation

Related Links • Conditioning Purified Membrane Proteins • Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus • Performing a purity and homogeneity check • Performing a small-scale expressio...

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Appendix 1, extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014 Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, reduce...

Protocol Guide: CRISPR/CAS9 Gene Editing of Human Induced Pluripotent Stem Cells (iPSCs)

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • RNA Reprogramming Kits • Human iPSC Media • Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media Filtration • Growth Factors • CRISPR Genome Editing Basics • ...

BioUltra Reagents Density Gradient

Introduction References Product List To meet highest demands for quality, we offer a wide range of BioUltra Reagents for density gradient centrifugation. Introduction Criteria for an ideal density gradient centrifugation medium are: ○ the additive must form a solution within the required density ran...

DNA Cleanup using illustra™ GFX Kits

GE Healthcare provides three silica-based kits for DNA cleanup. illustra™ GFX™ PCR and Gel Band Purification Kit purifies PCR products and other DNAs from solution or gel slices. This product is discussed in more detail below. illustra™ GFX 96 PCR Purification Kit purifies PCR products from solution...

Preactivated Magnetic Beads

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014 Preactivated magnetic beads are designed for covalent coupling of antibodies, aptamers, and proteins. After coupling is performed, proteins of interest can be affinity captured and enriched using immunop...

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

BugBuster® Protein Extraction Reagent has become a valuable tool for extraction of proteins from E. coli without the need for mechanical disruption. It is also known that supplementing BugBuster® (as well as its derivative, PopCulture™ Reagent) with a small amount of lysozyme enhances the efficiency...

CBILS© - A Revolutionary Tool for Ionic Liquid Synthesis

. Additional Information . Materials List A Revolutionary Tool for Ionic Liquid Synthesis: The use of CBILS© – Carbonate Based Ionic Liquid Synthesis – developed by the Austrian company proionic, offers one of the simplest, yet most elegant synthetic methods for the production of ionic liquids.1 The...

Post-Reaction Clean-Up Plates for DNA & RNA Purification

SigmaSpin Post-Reaction Clean-Up Columns SigmaSpin Sequencing Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean up of probe labeling reactions or small numbers of sequencing reactions. These columns can accept sample volumes up to 100 µl. Each column comes with a c...

Chloroplast Iso Kit

Description: The Chloroplast Isolation Kit provides a quick and efficient procedure to isolate intact chloroplasts from plant leaves. Intact chloroplasts are the best starting material for studies of chloroplastic processes such as carbon assimilation, electron flow and phosphorylation, metabolic tr...

HTS and Optimization of a Multimodal Polishing Step in a MAb Purification Process Using Capto Adhere

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013 This application describes the use of Capto adhere and MabSelect SuRe chromatography media to significantly reduce the level of IgG antibody aggregates in a sample using an efficient two-step method. The method resulted in high yiel...