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    Search term:"centrifugation"

    343 matches found for centrifugation

    Mammalian Genomic DNA Miniprep Kit

    For purification of genomic DNA from a variety of mammalian sources Description: The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources (Figure 1 and Figure 2). These kits use a silica-ba...

    Nanoarchitecture of Protective Coatings for Air Sensitive Metal Hydrides

    • • Download PDF • Institute for Micromanufacturing, Louisiana Tech University Hydrogen storage materials are being considered for safe on-board vehicle storage of H2 gas used to power proton exchange membrane fuel cells. There are various methods for storing hydrogen,1 which include high pressure s...

    DNA & RNA Purification:Minus EtBr Spin Columns

    Description: Description: GenElute™ Minus EtBr Spin Columns are based on the GenElute Agarose Spin Column but incorporate a membrane for the selective removal of ethidium bromide (Figure 1). Procedure: The DNA band is excised from an agarose gel and loaded onto the spin column. The membranes, embedd...

    Quick Spin Columns for radiolabeled RNA purification Troubleshooting

    Product No. 11273990001, 11274015001 Nucleotide contamination of filtered sample using mini Quick Spin Columns or Quick Spin Columns Possible reasons are: 1. Sample should not be applied to the side of the column, so that molecules to flow around the matrix; samples should be placed on top of the ma...

    Hematopoietic Stem Cell Culture

    Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter. Stromal Cell Lines For T-Cell Differentiation Hematopoietic Stem Cell Media and Cytokines Hematopoietic Stem Cell Characterization Stem Cell Culture Protocols He...

    Extract-N-Amp™ Blood Product Information

    The Extract-N-Amp Blood PCR kits contain all of the reagents necessary to rapidly extract genomic DNA from whole blood and amplify targets of interest by PCR (Figure 1). • Novel – single-step extraction of genomic DNA to PCR • Fast – tissue to PCR in 15 minutes • Convenient – no long enzymatic diges...

    Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

    Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016 Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step. GE o...

    Resuscitation of Frozen Cell Lines

    Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability of the culture and enable the culture to recover more quickly. Some cryop...

    Reverse Transcriptase AMV Protocol

    Product No. 10109118001 HANDLING OF RNA Short-term storage Resuspended RNA is best stored at -20 °C in RNase-free buffer TE buffer (10 mM Tris, 1mM EDTA) or RNase-free H2O (with 0.1 mM EDTA). Please note: It is important to use an EDTA solution known to be RNase-free for this purpose (older EDTA sol...

    Sequencing Clean Up in DNA & RNA Purification

    Click on the product for more information Product Speed Starting Material Product Description Technology Applications UltraClear™ Sequencing Reaction Clean-Up Kit Flexible based on user interface ≤10 µl sequencing reaction Removes unincorporated dye terminators, including BigDye version 3.1, dNTPs, ...

    Quick Spin High Capacity Columns Troubleshooting

    Product No. 03117928001 Nucleotide contamination of final sample for mini Quick Spin Columns or Quick Spin Columns Possible reasons would be: • Sample should not be applied to the side of the column, so that molecules flow around the matrix instead of through the matrix (without purification). Recom...

    Sample Preparation & Gel Electrophoresis: Western Blot Troubleshooting

    Related Links • Western Blotting (Immunoblotting) Protocol • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Sample Preparation Methods from GE Healthcare • Products for Cell Lysis and Protein Extraction • Western Blotting Home Page • Western Blotting Literature & Resources • We...

    Sample Labeling and Processing of Proteins

    Use freshly prepared extracts for protein labeling is highly recommended. Use of extracts from frozen tissues or cell lines with low viability, or old protein extracts may give inadequate results. For successful labeling, the extract should be clear. If small particles are observed, it is recommende...

    Troubleshooting & Frequently Asked Questions for HybridSPE-PL

    Yes. The HybridSPE-PL Small Volume plate (52794-U) is designed for processing plasma/ serum volumes between 20-40 µL. Larger sample volumes of 100-300 µL should be processed on the standard HybridSPE-PL plate (575656-U). Acetonitrile is a commonly used protein precipitation agent when prepping plasm...

    Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit

    Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regulatory small RNAs are divided into two main classes; (a) small interfering RNAs (siRNAs) are double-st...

    Preparation of Plasmid DNA Protocol

    Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. View the protocol on BioSupplyNet.com. Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell. Preparation of Plasmid DNA by Alkaline Lysis with...

    Purification Using GST SpinTrap

    GST SpinTrap columns are designed for rapid, small-scale purification of GST-tagged proteins using mild conditions of affinity purification. More than 90% purity can be achieved in a single step. The columns are suitable for purification of multiple samples in parallel, for screening experiments, or...

    5'/3' RACE Kit, 2nd Generation Troubleshooting

    Product No. 03353621001 POOR RESULTS In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction, ...

    Forskolin from Coleus forskohlii

    CAS RN 66575-29-9 Synonyms: Coleonol, Colforsin T-cell activation is normally triggered by the interaction of a cell surface receptor to its specific ligand molecule. This binding event triggers the rapid hydrolysis of inositol phospholipids to diacylglycerol and inositol phosphates by phospholipase...

    Veterinary Drug Residues

    Related Articles • Molecularly Imprinted Polymer SPE for the Highly Selective Extraction of Fluoroquinolones from Bovine Kidney • Extraction of Nitroimidazoles from Milk and Eggs using Molecularly Imprinted Polymers Related Links • Analytical Standards, Antibiotics • Certified Reference Materials (C...

    Pellet Paint NF Co-precipitant: Non-fluorescent Visible DNA Co-precipitant for Automated Sequencing and Other Applications

    Pellet Paint NF CoPrecipitant* is a nonfluorescent dye labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye™ Terminators (PE Corp.) during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly...

    Total RNA Purification using illustra™ RNAspin Mini and Midi RNA Isolation Kits

    Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 The following protocol summarizes use of the illustra™ RNAspin Mini and Midi RNA Isolation Kits. Most of the materials and preparations are the same for the RNAspin 96 Kit, but the RNAspin 96 Kit includes one...

    How to make and use gradients of Percoll

    Extracted from Cell Separation Media Methodology and Applications, GE Healthcare, 2007 In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions. Adding 9 p...

    Plasmid DNA purification kits by Sigma Aldrich

    Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a complex, prolonged, and labor-intensive procedure. Nucleic acid purification technologies now give hi...

    Cyclosporin A from Tolypocladium inflatum

    CAS RN 59865-13-3 Synonyms: Cyclosporine; Antibiotic S 7481F1; Ciclosporin; CsA Cyclosporin A is a non-polar cyclic oligopeptide produced by the fungus Tolypocladium inflatum. It is a potent immunosuppressive agent, affecting primarily T-lymphocytes.2 It has been shown to inhibit the functioning of ...

    Anti-HA Affinity Matrix Protocol & Troubleshooting

    Product No. 11815016001 Concentrated Eluate To concentrate the protein eluate, Roche recommends following the immunoprecipitation protocol given in the package insert from step 2 to 5. Use 50 μl of resuspended Anti-HA Affinity Matrix in a microcentrifuge tube. Take the wash and elution buffer as des...

    A Comprehensive Workflow Real-time Analysis of Cell Migration

    Amedeo Cappione, Senior Scientist | Cindy Chen, Scientist | Charles Neville, Scientist | Timothy Nadler, R&D Manager Cell migration is directed by the interaction of cells with the extracellular matrix (ECM), neighboring cells, and biomolecules present in the milieu. Cell migration contributes to bo...

    Improve LC-MS Performance | HybridSPE

    When analyzing biological samples via reversed-phase LC-MS, ion-suppression often occurs in the early (e.g., salts) and late regions (e.g., phospholipids) of a chromatogram. However, as analysts strive for faster run times (=3 min.), resolving analytes of interest from interfering matrix components ...

    Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

    Download BioFiles v6 n5 (4.66 Mb PDF) The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate a...

    3D Neural Stem Cell Models of Alzheimer’s Disease Protocol

    Introduction Protocol • Human ReNcell® VM NSC Maintenance and Passaging • Viral Infection of Human ReNcell® VM NSCs • Enrichment of High-Expressing Transgenic Human ReNcell® VM NSCs • Culturing Sorted Human ReNcell® VM NSCs • 3D Matrix Cultures of Human ReNcell® VM NSCs • 3D Culture Cell Seeding of ...