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126 matches found for chromatin_immunoprecipitation

Webinar: 3D Organ-on-a-Chip Applications Using the AIM Biotech Chip

In vitro 3D cell culture models have emerged as a bridge between conventional 2D cell culture models and the complex and expensive in vivo animal models. By observing, analyzing and comparing the biological behavior of tissues embedded in 3 dimensional hydrogels, results are significantly different ...

Potential Application for 5-Methylquinoxaline, FG

Available in Kosher and Halal There is a toasted nut character with lingering roasted corn notes that would work well in corn chips as well as graham crackers, imparting a toasted cereal note. At higher concentrations, this ingredient would be beneficial in roasted caramel, cocoa or coconut flavors....

Imprint® Chromatin Immunoprecipitation Kit - ChIP Kit

The Fastest and Most Convenient ChIP Kit Available! Sigma combines speed and convenience with performance in the Imprint Chromatin Immunoprecipitation Kit (CHP1). The Imprint ChIP Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification a...

Restriction Enzyme Cloning Glossary

3’ end / 3’ 3’ UTR / 3’ Un-translated region 5’ Cap /Cap 5’ end / 5’ 5’ UTR / 5’ Un-translated region Acrylamide Gel Agarose Gel Amp+/Kan+/Tet+/Chl+/Spc+/Str+ Ampicillin Antibiotic Resistance Assay ATG BAC Bacteriophage Blunt end BP / Base pairs CCAAT Box cDNA cDNA clone cDNA library ChIP assay Chlo...

Redi-Dri™ – The world of free-flowing hygroscopic products

The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times. Don’t waste valuable time and resources using reagents that readily absorb environmental moisture and harden pr...

Quantum Dots for Electronics and Energy Applications

What are Quantum Dots? Perovskite Quantum Dots PbS Quantum Dots Quantum Dot Applications Light Emitting Diodes (LEDs) Lighting Solar Cells Photodectors Biomedical Imaging Quantum dots (QDs) are nanometer sized (~2-10 nm) semiconducting material. Owing to their small size, QDs exhibit quantum confine...

Chromatin Immunoprecipitation (ChIP)

One of the most powerful tools available for investigating epigenetic mechanisms of disease is chromatin immunoprecipitation (ChIP). ChIP can detect and relatively quantify specific protein-DNA and protein- protein interactions in vivo at a single or multiple loci. ChIP involves chemically crosslink...

How to Clean Laboratory Glassware

Related Links • Laboratory Cleaning Supplies • Glassware • • Download PDF • Introduction Cleaning Glassware Cleaners Safe Use of Chromic Acid Removing Grease Rinsing Sterilizing Contaminated Glassware Handling and Storing Autoclaving-Cloudiness Cleaning Labeling and Marking Burets Culture Tubes Dish...

Guides to cell number for ChIP and endpoint analysis

ChIP can be performed on either cultured cells or tissues. In either case, it is important to consider the homogeneity of your sample, as each contributing cell type may have a different chromatin structure. It is also important to determine how many ChIP reactions you will be performing. The number...

ChIP Tested Antibodies

Chromatin structure plays a fundamental role in regulating processes that take place on the DNA, such as transcription, replication, and recombination, all of which occur on nucleosomal templates. Therefore, mapping the position of histones selectively modified and histone variants or non-histone co...

A Quantitative Mass Spectrometry-Based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity

• • Download PDF • Huang et al.1 have recently developed a quantitative mass spectrometry-based assay for identifying protein kinase substrates and quantifying kinase activity. This approach offers several advantages over current methods: • It is label-free and eliminates the need for fluorescent or...

Hypoxia Detection Assays

Related Products • Cellular Assays • Cell Culture Media • Fetal Bovine Serum • Cell Culture Reagents • Cancer Cell Lines • Primary Cell Culture • 3D Cell Culture • Live Cell Imaging Reagents • CellASIC® Microfluidic Live Cell Imaging System • PHK Cell Labeling Dyes • Live Cell Fluorescent Dyes • Ang...

3D Cell Culture Tools and Applications

Cultivate reality with advanced 3D cell culture models. 3D cell culture techniques have led to the creation of more predictive in vitro cell models for numerous applications including cancer research, drug discovery, neuroscience and regenerative medicine. Cells naturally grow and differentiate in t...

Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of those research papers. Other citations are available under the specific product pages for each of our...

A MALDI-based Chip Technology for Single-Cell Mass Spectrometry (SCMS)

Heterogeneity is a characteristic attribute of all populations of living organisms. Cell-to-cell variability can be due to a number of reasons; e.g., non-uniformity of the cellular environment, such as a given cell within the cell cycle, genetic differences resulting from mutations or stochastic eve...

ChIP Troubleshooting Questions

• On page 3 of the bulletin, the tissue prep procedure calls for making the cross-link solution with formaldehyde in cell culture medium but there is no mention of cell culture medium anywhere else and why would this be used instead of an extraction buffer? What else can be used? • What if there are...

Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. Al...

Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit

Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regulatory small RNAs are divided into two main classes; (a) small interfering RNAs (siRNAs) are double-st...

UPS1 and UPS2 Proteomic Standards

We now offer both the Universal Proteomics Standard and the Proteomics Dynamic Range Standard as complex, well-defined, well characterized reference standards for mass spectrometry. Both standards contain the same 48 human proteins ranging in molecular mass from 6,000 to 83,000 Daltons. Each constit...

Astec Chiral Chromatography Bibliography - Recent Citations

Copyright restrictions do not permit us to provide copies of the articles listed here. A direct HPLC method for the resolution and quantitation of the R-(-)- and S-(+)-enantiomers of vigabatrin (γ-vinyl-GABA) in pharmaceutical dosage forms using teicoplanin aglycone chiral stationary phase. Al-Majed...

Binding and Kinetic Analysis of Protein Interactions Probed by Label-Free SpotMatrix SPR Technology in a Peptide Chip Format

• • Download PDF • Protein-protein interactions play central roles in almost all cellular responses. Specific interactions between peptide motifs and signaling domains such as SH2 and WW domains are critical for a variety of signaling pathways. Many techniques have been developed to study protein bi...

Chemical Deposition Techniques in Materials Design

• • Download PDF • Sigma-Aldrich Materials Science During the last decade, chemical deposition techniques have played an exceptionally important role in the design and manufacture of novel advanced devices. Physical deposition techniques, such as molecular beam epitaxy (MBE), physical vapor depositi...

Read the latest developments in CRISPR research

read abstract Unconventional Trafficking of Mammalian Phospholipase D3 to Lysosomes Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15 23-01-2018 Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late...

Sample Purification & Quality Assessment - PCR Technologies Guide

• Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR qPCR dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

Agarose beads Vs. Magnetic beads in ChIP

The ChIP antibody may be conjugated directly to agarose or magnetic beads or it may be immobilized on beads conjugated to protein A and/or protein G. Original ChIP protocols used agarose beads, but many long time ChIP users have moved to magnetic beads. Magnetic beads enable rapid isolation of prote...

Genome engineering using the CRISPR-Cas9 system

Nature Protocols Volume 8 Issue 11, 24 November 2013, Pages 2281 - 2308 doi: 10.1038/nprot.2013.143 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Sc...

Biology and Analytical Chemistry Webinar Guide

Biology Webinar Guide Quality Systems and Regulatory Compliance Drug Discovery Webinar Guide Analytical Webinar Guide Materials Science Webinar Guide • Proteomics • qPCR • CRISPR • Cell Culture • Cell Design Studio • Duolink • RNAi • Regulatory Compliance • Late Stage Functionalization of Drug-Like ...

Acrylamide in Food: Kits, columns and tubes for sensitive GC-MS and LC-MS analyses and solid phase extraction

Rainer Walz PhD, Product Manager An Trinh, Product Manager, Supelco Acrylamide is a commonly analyzed food-borne carcinogen. Sigma-Aldrich has convenient kits, LC and GC columns and sample prep methods for fast and reliable analysis of acrylamide by both analytical techniques. Acrylamide analysis ha...

Commercial Volumes of Quantum Dots: Controlled Nanoscale Synthesis and Micron-Scale Applications.

• • Download PDF • Nanoco Technologies, Ltd., Manchester, United Kingdom Quantum dots (QDs) are luminescent semiconductor nanoparticles, with diameters in the range of 1 to 20 nm. The unique optical and electronic properties of QDs are being exploited in a number of applications including flat panel...

Cold Spring Harbor Laboratory Protocols

We have prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. The product lists correspond to protocols for: • Antibodies • RNAi • Genomics • Neuroscience • Proteomics • Stem Cell Biology • Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography F...