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    Search term:"chromatin_immunoprecipitation"

    104 matches found for chromatin_immunoprecipitation

    DNA & RNA Purification: mRNA and GenElute Direct mRNA Miniprep Kit

    Description Features and Benefits RNA Kits Kit Contents Preparation Time Comparison RNA Yield Specific mRNA Yield mRNA—Suitable for Northern Blots Description Procedures such as cDNA synthesis, expression profiling, and others, require separation of mRNA from the vastly more abundant rRNA and tRNA. ...

    Target Identification & Validation for Early Drug Discovery

    Early stages of drug discovery start with initial steps of target identification and moves to the later stages of lead optimization. Multiple sources including academic research, clinical works and commercial sector help in the identification of a suitable disease target. The chosen target is then u...

    Vitamin D, Multiple Sclerosis Connection

    Download this article (pdf 3.46MB) Abstract Multiple sclerosis (MS) is a complex trait in which allelic variation in the MHC class II region exerts the single strongest effect on genetic risk. Epidemiological data in MS provide strong evidence that environmental factors act at a population level to ...

    Non-Organic-Based Isolation of Plant microRNA using Norgen's Plant/Fungi RNA Purification Kit

    Small RNAs, including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are key components of an evolutionarily conserved system of RNA-based gene regulation in eukaryotes. In eukaryotes, regulatory small RNAs are divided into two main classes; (a) small interfering RNAs (siRNAs) are double-st...

    Building and Engineering Micro/Nano Architectures of Single-Walled Carbon Nanotubes for Electronic Applications

    1Department of Mechanical and Industrial Engineering, Northeastern University, Boston, MA 02115, USA 2Department of Physics, Northeastern University, Boston, MA 02115, USA 3Department of Mechanical Engineering, Villanova University, Villanova, PA 19085, USA *Email: y.jung@northeastern.edu Single-wal...

    Fluorescence Lifetime Measurement Advantages & Applications

    Fluorescence lifetime (FLT) is the time a fluorophore spends in the excited state before emitting a photon and returning to the ground state. FLT can vary from picoseconds to hundreds of nanoseconds depending on the fluorophore. The lifetime of a population of fluorophores is the time measured for t...

    A Quantitative Mass Spectrometry-Based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity

    • • Download PDF • Huang et al.1 have recently developed a quantitative mass spectrometry-based assay for identifying protein kinase substrates and quantifying kinase activity. This approach offers several advantages over current methods: • It is label-free and eliminates the need for fluorescent or...

    Proteomics & Protein Expression Poster Gallery

    Featured Poster Use of an Animal-free Synthetic Surrogate Serum Matrix for Preparation of Calibrators and Controls in Immuno- and LC-MS Based Clinical Assays Click on the quick links below to see posters on each topic • Mass Spectrometry Tools • ProteoPrep™ Plasma Immunodepletion • NEW! SILu™Prot Ma...

    Perovskite-phase Metal Oxide Nanostructures: Synthesis, Properties, and Applications

    Yuanbing Mao1, Hongjun Zhou1, and Stanislaus S. Wong1,2,* 1Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400 *Email: sswong@notes.cc.sunysb.edu 2Condensed Matter Physics and Materials Sciences Department, Brookhaven National Laboratory, Upton, NY 11973 ...

    The Sigma-Aldrich Microreactor Explorer Kit — Made by Chemists for Chemists

    • • Download PDF • All-in-One Solution The Sigma-Aldrich Microreactor Explorer Kit was designed to give newcomers an opportunity to start discovering this innovative technology right away. Designed by chemists for chemists, it contains everything that is needed to perform flow chemistry experiments ...

    Cold Spring Harbor Protocols for Epigenetics Research

    Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications View the protocol on BioSupplyNet.com Yeast Chromatin Immunoprecipitation (ChIP) Assay

    Read the latest developments in CRISPR research

    read abstract Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting. Chen Fuqiang, Ding Xiao, Feng Yongmei, Seebeck Timothy, Jiang Yanfang, Davis GregoryD Nature Communications 2017-04-01 Bacterial CRISPR-Cas systems comprise diverse effector en...

    Chemical Deposition Techniques in Materials Design

    • • Download PDF • Sigma-Aldrich Materials Science During the last decade, chemical deposition techniques have played an exceptionally important role in the design and manufacture of novel advanced devices. Physical deposition techniques, such as molecular beam epitaxy (MBE), physical vapor depositi...

    Imprint® Methylated DNA Quantification

    The Imprint Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. Compared to the conventional methods of analyzing hypo- and hyper- global methylation like reverse HPLC, MALDI-TOF-MS, TLC, etc. Sigma’s Imprint. Methylated DNA Quantif...

    Binding and Kinetic Analysis of Protein Interactions Probed by Label-Free SpotMatrix SPR Technology in a Peptide Chip Format

    • • Download PDF • Protein-protein interactions play central roles in almost all cellular responses. Specific interactions between peptide motifs and signaling domains such as SH2 and WW domains are critical for a variety of signaling pathways. Many techniques have been developed to study protein bi...

    Cold Spring Harbor Laboratory Protocols

    Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. The product lists correspond to protocols for: • Antibodies • RNAi • Genomics • Neuroscience • Proteomics • Stem Cell Biology • Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatograph...

    Retinoic Acid and Gene Expression

    • • Download PDF • All-trans retinoic acid (RA, ATRA) is a pleiotropic activation factor that regulates genes associated with normal vertebrate cellular processes such as cell differentiation, cell proliferation, apoptosis, and embryonic development. Retinoic acid and the receptor proteins for which...

    Parkinson's Disease

    Download BioFiles v7 n2 (13.0 Mb PDF) Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurodegeneration characterized by akinesia, rigidity and resting tre...

    Water for Electrophysiology

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    Transplex® RNA Amplification Kit: Whole Transcriptome Amplification of Highly-Degraded RNA from FFPE Tissues.

    Ken Heuermann and Brian Ward Research and Development, Biotechnology Division, Sigma-Aldrich The TransPlex Complete Whole Transcriptome Amplification (WTA2 ) Kit effectively amplifies intact and highly degraded RNA. To benchmark maintenance of representative RNA levels during amplification, differen...

    Gold Nanoparticles: Properties and Applications

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    Redi-Dri™ – The world of free-flowing hygroscopic products

    The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times. Don’t waste valuable time and resources using reagents that readily absorb environmental moisture and harden pr...

    Isolation of Plant and Fungal RNA from Challenging Samples using Norgen's Plant/Fungi RNA Purification Kit

    Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. Al...

    Custom Peptide Library | PEPscreen®

    Related Links • Peptide Portfolio • Quotation Request FAQ Peptide Library Stability Storage & Handling Guidelines Quotation Request Order Now Experience affordable and fast delivery of custom peptide libraries for target identification. The PEPscreen peptide library is configured in a convenient pla...

    Downstream Applications after Genomic DNA Purification

    Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 The following table summarizes the general quantity and purity considerations for several common applications for genomic DNA. Table 4.3. Summary of downstream applications for genomic DNA and substances that...

    Rapid SYBR® Green qPCR on the Illumina Eco™ Real-Time PCR System

    Related Content • Kapa Genomics Reagents • Roche Biochemical Reagents Fast real-time PCR is a demanding application that requires consistent and reproducible results from the difficult amplicons and low-volume reactions. The combination of the Illumina Eco™ Real-Time PCR System and KAPA SYBR® FAST q...

    Quantum Dots for Electronics and Energy Applications

    What are Quantum Dots? Perovskite Quantum Dots PbS Quantum Dots Quantum Dot Applications Light Emitting Diodes (LEDs) Lighting Solar Cells Photodectors Biomedical Imaging Quantum dots (QDs) are nanometer sized (~2-10 nm) semiconducting material. Owing to their small size, QDs exhibit quantum confine...

    Tutorial in Nanomaterials

    Introduction Nanotechnology Nanotools Nanodevices Nanostructured Materials References Although the idea of carrying on manipulations at smaller and smaller scales has been around for quite some time the birth of nanotechnology, at least on an ideological level, is usually traced back to a speech by ...

    Imprint® Ultra Chromatin Immunoprecipitation Kit

    Sigma’s second generation chromatin immunoprecipitation (ChIP) kit was developed for maximum sensitivity and optimum next-generation sequencing results. The DNA-blocked “Staph-Seq” cells are ideally suited for studying recruitment of low abundance transcription factors (TF) in genome wide location a...