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270 matches found for cloning

Nucleic Acid Electrophoresis & Hybridization

Sigma-Aldrich offers a wide variety of products for nucleic acid electrophoresis, hybridization and detection procedures. For Northern and Southern blotting we have products for every stage of the workflow. In addition, the Learning Center provides links to free handbooks, selection guides and proto...

Agarose and Acrylamide product lines for nucleic electrophoresis

Agarose Learning Center Agarose Pre-cast Agarose Gels Acrylamide Agarose We offer a range of molecular biology grade agarose products from routine use to wide range and low melt point agarose. Each lot of products is rigorously tested to ensure reproducibility from one lot to the next. Use the follo...

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and proteins that are at work within the cell. Molecular Biologists use some standard techniques that have ...

10x Uracil Cleavage System

Catalog Number KEM0011 Storage Temperature –20 °C Unit Size 750 Reactions The Uracil Cleavage System provides two enzymes, which, when added sequentially to a reaction containing a synthetic DNA fragment containing a deoxy-uracil, generate a single nucleotide gap at the location of the uracil residu...

Successful Amplification of Thermally Degraded DNA Using PCRboost™

Introduction DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions. The common end result of expos...

ABC Transporters and Cancer Drug Resistance

• • Download PDF • Role of ABC Transporters and Multi-Drug Resistance Reversal Using RNA Interference (RNAi)* Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a...

CHOZN® Platform Technical Bulletin

• • Download PDF • Product Description/Overview Precautions and Disclaimer Storage and Stability Background Part I: Medium Preparation Part II: Stock culture initiation/thawing and subculturing Part III: Stock culture maintenance Part IV: Cell banking Part V: Transfection Part VI: Stable pool select...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...

GenElute HP Endotoxin-Free Maxiprep Kit Article

By Dave Cutter and Goodarz Goodarzi Sigma-Aldrich Corporation, St. Louis, MO, USA View pdf version (209 KB) On this page: • Introduction • Materials and Methods • Results and Discussion • Conclusions • Acknowledgements • References • Ordering Information Introduction Plasmid DNA purification kits th...

endogenous retroviral pol gene-like sequence 1 (clone HLM2)
Synonyms HLM2
Species Human (2082)

Alkaline Phosphatase Detection Kit, Fluorescence

Catalog Number: APF Storage Temperature –20 °C Product Description Components Equipment and reagents required but not provided Precautions and Disclaimer Storage/Stability Procedure Troubleshooting Materials The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to det...

DNA & RNA Purification GenElute Agarose Spin Columns

Description: GenElute™ Agarose Spin Columns isolate DNA by centrifugation and filtration. Procedure: A slice of agarose gel containing the DNA of interest is excised, placed into a spin column, and spun at maximum speed in a microcentrifuge. Agarose is retained on a filter in the spin column. Solubl...

Bacterial Transformation

Related Articles • Transforming E. coli with Engineered Plasmid • Transformation Protocols • Introduction to Blue-White Screening • Preparation of Competent Cells and Transformation with pGEX DNA Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign...

Glutamate Receptors (G Protein Family)

The existence of G protein-coupled glutamate receptors (also called "€œmetabotropic" glutamate or mGlu's), belonging to the seven transmembrane spanning superfamily of receptors, was shown definitively with the cloning of the first member in 1991. Since then, eight receptors of this class have been ...

shRNA Frequently Asked Questions for TRC Design and pLKO.1 Vector Use

Why is the U6 promoter used? What restriction sites were used for cloning the shRNA sequences? Does pLKO.1 contain WPRE? What restriction enzymes are recommended for diagnostic digests of the clone DNA? How large of an insert can pLKO.1 handle? If I order TRC1.5 clones and controls, will they work w...

KAPA2G Fast PCR Kits FAQ

What are the recommended applications for KAPA2G Fast PCR Kits? • Standard, end-point PCR assays normally performed with wild-type Taq (or hot-start formulations thereof) – but completed in 20%–70% less time • High-throughput end-point PCR • End-point PCR for genetic testing or downstream Sanger seq...

Evaluation of DNA Extraction and Amplification Protocols to Investigate Bacterial Communities on Cultural Heritage

1A. Meloscia, M. Orsini, 1G. Lustrato, and 1G. Ranalli 1DISTAAM, Università degli Studi del Molise via De Sanctis, 86100 Campobasso (I) Correspondence to: Giancarlo Ranalli, DISTAAM, Università degli Studi del Molise via De Sanctis, 86100 Campobasso, Italy. Tel. +39-0874-404604 (fax –652). E-mail: r...

Protein Expression Systems

Click here to return to the Molecular Biology Guide. The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant p...

RT-PCR | Reverse Transcriptase PCR

RT-PCR, or Reverse transcriptase PCR, is a variation of standard PCR technique, which involves the amplification of specific mRNA obtained from very small samples. It eliminates the need for the tedious mRNA purification process required for conventional cloning techniques. In RT-PCR, reverse transc...

KAPA2G Robust PCR Kits FAQs

What are the recommended applications for KAPA2G Robust PCR Kits? • Amplification of templates with high GC or AT content • Amplification of templates containing common PCR inhibitors at levels inhibitory to wild-type Taq • Amplification from crude samples, e.g. buccal swabs; cultured mammalian, bac...

Post Reaction Cleanup in DNA & RNA Purification

We provide GenElute™ PCR clean-up, agarose spin columns and minus EtBr spin columns for purification of single or double stranded PCR amplification fragments; as well as extraction of DNA fragments from Ethidium Bromide (EtBr) stained agarose gels. Applications for these kits would include enzymatic...

New Lab Start-Up Product Savings

Sigma-Aldrich New Lab Start-Up Program – Product Savings With the Sigma-Aldrich New Lab Start Up Program, you will receive: Savings on Essential Life Science Products Cell Culture Discounts (up to) Liquid Media* 50%* Powdered Media 20% Antibiotics 15% Growth Factors 10% Reagents 20% PCR Reagents Dis...

Starting materials needed for the purification of integral membrane proteins for structural and functional studies

Related Links • Cell disruption and membrane preparation • Conditioning Purified Membrane Proteins • Performing a purification and on-column refolding of an insoluble histidine-tagged protein from a 100 ml E. coli culture using HisTrap FF 1 ml with ÄKTAprime plus • Performing a purity and homogeneity...

Whole Genome Amplification for Single Cell Biology - Life Science Innovations

• • Download PDF • Sigma-Aldrich Corp., St. Louis, MO, USA The ultimate biological unit lies within a single cell. Many biological disciplines have taken aim to elucidate the causes of cellular differentiation at this level. The secret triggers that signal human maturation, regeneration and genetic ...

An Introduction to Antibodies: Generation of Antibodies

Antibody Formats Biological Effects of Antibodies Antibodies are normally produced by B cells, which are part of the immune system, in response to the introduction of foreign substances, such as infectious agents, into the animal’s body. The antibodies bind to the antigens that cause their generatio...

Reprogramming Human Fibroblasts using the Dox Inducible Reprogramming Polycistronic Lentivirus Set: Human 4F2A LoxP

• • Download PDF • The following protocol describes the reprogramming of one well of BJ Human Fibroblasts (BJ cells) into induced pluripotent stem (iPS) cells in a 6-well format. Transduction efficiency can vary depending on such factors as the amount of virus used, the type of cell transduced, the ...

Automated High Throughput Method for Plasmid DNA Purification

Simple, rapid, high-throughput purification of transfection quality plasmid DNA in less than 50 minutes The GenElute™ HP 96 Well Plasmid Miniprep Kit has been developed for use as a high-throughput method for the purification of plasmid DNA from recombinant E. coli cultures. This rapid and cost-effe...

x-tracta Gel Extraction Tool

The x-tracta gel extraction tool is an easy to use, quick alternative to using scalpel or razor blades for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis. The cutting blade and ergonomic design mean that extractions can be performed in a rapid, easy to follow pro...

Glycine Receptors

The amino acid glycine is a major inhibitory neurotransmitter in the vertebrate CNS. Glycinergic synapses are particularly abundant in spinal cord and brain stem, but are also found in higher brain regions including the hippocampus. The inhibitory actions of glycine are potently blocked by the alkal...

MSX Amplification

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992). However, most Chinese Hamster Ovary (CHO) cell production lines contain endogenous GS, and Methionine Sulfoximine (MSX) is required to inhibit e...

Procedures for Dephosphorylation

Procedures for Dephosphorylation Procedure Recommended Product 1. Dephosphorylation of DNA using Calf Intestinal Alkaline Phosphatase P4978 2. Dephosphorylation of DNA using Shrimp Alkaline Phosphatase P9088 3. Dephosphorylation of Protein using Bovine Intestinal Alkaline Phosphatase P0114 1. Dephos...