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115 matches found for detection_methods

High Purity SWNTs: Electronic & Biomedical Applications

Carbon Nanotubes (CNTs) are stable, hollow cylinders of pure carbon closely related to graphite, graphene and fullerenes.1 CNTs can be visualized by taking a single graphene sheet and rolling it into a tube with a diameter on the nanometer scale. CNTs are commonly classified into two categories, sin...

The MISSION® Target ID Library for Human miRNA Target Identification and Discovery

Carol A. Kreader and Kevin P. Forbes One of the main problems miRNA researchers face today is global identification of gene targets that are functionally regulated by their miRNA of interest. Unfortunately, bioinformatic predictions are unreliable and there is little agreement between different algo...

Class-Selective Enantiomeric Separation of beta-Blockers and beta-Agonists using CHIROBIOTIC™ T Stationary Phase

Daniel Shollenberger daniel.shollenberger@sial.com Understanding the role of chirality in pharmacology became extremely important following the tragedy of thalidomide use in the 1960s. At that time the effect of chirality was poorly understood, and whilst it was discovered that the sedative effect r...

NIOSH 5029 (Issue 1)– Products and Materials Guide

DEMETON The method is specific for MDA and the use of UV and electrochemical detectors in serie s gives greater reliability to the analysis, and allows a wide measurement range. The method has been applied to wipe samples. Analysis Type (Application Method): HPLC, UV and electrochemical Link to NIOS...

Milliflex Oasis® Filtration System for Bioburden and Pharmaceutical Water Testing

Membrane filtration is the method of choice for water and bioburden testing. We have taken time to closely observe your complete laboratory workflow to identify improvement areas and developed, hand-in-hand with users, a solution that simplifies and secures your microbial testing process. Vacuum pum...

Analysis of Metabolites by HPLC

Related Articles Fast HPLC Using Fused-Core® Particle Technology for Rapid Screening of Pharmaceutical Compounds Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 Alternative Retention and Selectivity Us...

MISSION® shRNA Guarantee

Based upon the design algorithm developed by The Broad Institute, upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest in viral format (this varies, but is typically 5 clones), we guarantee that at least one of those viral clones for a gene should yield ...

Lateral Flow Tests for Immunological Detection in Food and Environmental Samples

Lateral flow tests are immunoassays for detecting food pathogens with the ultimate convenience. Covering the major pathogens, the tests act as mini-laboratories and include a built-in control reaction for optimized pathogen testing. Definitive results are delivered in as little as 20 minutes. Immuno...

SPME in the Analysis of Illicit Drugs

Related Articles • SPE Method to Eliminate Matrix Effect in Analysis of NBOMe Hallucinogens from Urine • Utilizing Novel SPME LC Tips for Forensic Screening of Drugs of Abuse in Urine Samples • A Tryptamine Comeback: Interest Beyond Illicit Use • LC-MS of Illicit Bath Salts in Urine • Accelerated Bi...

Oligonucleotide Quality Control by Analytical HPLC

Related Articles • Oligo QC & QA • QC by Mass Spectrometry • QC by UV Spectroscopy • QC by PAGE Analysis • QC of Custom Formats • QMS Components • Quality Registrations RP-HPLC (reverse-phase high-performance liquid chromatography), IE-HPLC (ion-exchange high-performance liquid chromatography) and U...

Digital PCR - PCR Technologies Guide

PCR Technologies Guide Historical Timeline Polymerase Chain Reaction Quantitative PCR Quantitative PCR and Digital PCR Detection Methods PCR/qPCR/dPCR Assay Design Sample Purification and Quality Assessment Reverse Transcription Assay Optimization and Validation Data Analysis Troubleshooting Regulat...

Determination of Bisphenol A in Drinking Water

The level of bisphenol A (BPA) detected in foods and beverages has gained media attention over the last several years. Specifically, this interest is related to two types of containers: • Re-useable rigid containers made of polycarbonate plastic; commonly used for water bottles, baby bottles, plasti...

Water for Clinical Chemistry

In the clinical laboratory, water is a key factor. There are two significant reasons for the importance of water in the clinical setting: the need to comply with norms or guidelines (e.g., those of the Clinical and Laboratory Standards Institute® – CLSI®)1 and the sensitivity of the chemistries them...

Fluorescence Lifetime Measurement Advantages & Applications

Fluorescence lifetime (FLT) is the time a fluorophore spends in the excited state before emitting a photon and returning to the ground state. FLT can vary from picoseconds to hundreds of nanoseconds depending on the fluorophore. The lifetime of a population of fluorophores is the time measured for t...

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and proteins that are at work within the cell. Molecular Biologists use some standard techniques that have ...

Cell Signaling & Neuroscience Cell Biology Assays

Cell Biology Assay Kits Sorted Alphabetically Sorted by Area of Interest Sorted by Platform Formats Kit Platform Descriptions Features and Benefits of Selected Assay Kits Think Inside the Box Sigma is committed to providing its customers with the broadest available offering of innovative Cell Biolog...

Assurance® GDS Services

Pathogen testing using the Assurance® GDS system As increasing numbers QC labs adopt rapid detection methods to monitor microbial contamination in food products, we can help you to implement our Assurance® GDS rapid detection system and related consumables in a fast and efficient way. Let’s work tog...

Enzymatic Assay Kits for Food and Beverage Testing

We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis. Like many other analytical methods, enzymatic analysis was used first in clinical diagnosis, then adapted for the determination of food ingredients. The high specificity of enzymes enables the ana...

Chiral Chromatography FAQ

Chirality & Chiral Chromatography Chiral HPLC Column Selection & Screening Astec CHIROBIOTIC Chiral HPLC Astec CYCLOBOND Chiral HPLC Preparative Chiral HPLC Tips and Tricks for Chiral HPLC Chiral GC What is chirality? Chirality belongs to the discipline of stereochemistry, which is the study of the ...

GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011 Western blotting, also known as immunoblotting, is a well established and widely used technique for the detection and analysis of proteins. The method is based on building an antibody:protein compl...

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and the detection involves exposure to X-ray or autoradiography film in the dark. In western blotting, the ...

Applications Using Peptide Libraries

Related Links • Peptide Portfolio • Quotation Request Several applications in the area of drug design and drug delivery utilize peptide libraries. Select a link on our interactive display for descriptions and publications for the highlighted peptide library applications. Application Description Refe...

Choosing the Proper Ferrules for Your Capillary GC Column

Choosing the Proper Ferrules for Your Capillary GC Column Each GC instrument manufacturer requires a ferrule with different dimensions; hence, you may find that ferrules are usually not included in the box with a supplied capillary column. Additionally, mass spectrometer detectors may require the us...

Cell Culture Protocol 9: Testing Cells for Mycoplasma Contamination by Culture Isolation

Related Links • Cell Culture Homepage • Fundamental Techniques in Cell Culture- ECACC Handbook • ECACC Cell Lines • Fetal Bovine Serum • Classic Media and Salts • Cell Culture Reagents • Cell Culture Supplements • Stericup® Media Filtration • Primary Cell Culture • Stem Cell Culture • 3D Cell Cultur...

Solvent Center - Let us help you find just the right solvent for your specific need.

We are committed to providing our customers with the right solvent for their specific application. The Solvent Center was created to aid all visitors in their exploration of the right solvent by providing a concise summary of solvents by purity, grade, application, product lines or market. Packaging...

Surface Enhanced Raman Spectroscopy (SERS)

In last decades, surface-enhanced Raman spectroscopy (SERS) has been extensively explored and developed as an emerging technology to detect various chemical and biological analytes in environmental, agricultural, food, and medical applications1-7. SERS combined Raman spectroscopy and nanotechnology....

Regulatory Agencies & Conclusions - PCR Technologies Guide

PCR Technologies Guide Historical Timeline Polymerase Chain Reaction Quantitative PCR Digital PCR Quantitative PCR and Digital PCR Detection Methods PCR/qPCR/dPCR Assay Design Sample Purification and Quality Assessment Reverse Transcription Assay Optimization and Validation Data Analysis Troubleshoo...

Serum-Free 3D Spheroid Media

Related Products • 3D Cell Culture Tools and Applications • 3D Scaffolds • 3D Hydrogels • Stem Cell Culture Basics: Media, Cell Lines and Culture Reagents • The Cancer Stem Cell Hypothesis • Stem Cell Protocols • ECACC Cell Lines • Cell Culture • Growth Factors and Cytokines • Extracellular Matrix P...

General Considerations for Purification of GST-tagged Protein

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used. Table 3.2 shows cell culture, medium, and buffer volumes for obtaining an average yield ...

Apoptosis Assays

Related Links • Cellular Assays • Cell Viability and Proliferation Assays • Autophagy Assays • Cell Culture Media • ECACC Authenticated Cell Lines • 3D Cell Culture • Primary Cell Culture • Stem Cell Culture • Doulink™ PLA Protein Interaction Assays • Research Antibodies • Apoptosis Inhibitors • Apo...