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290 matches found for detergents

Avanti® FAQ

Drug Delivery / Pharmaceutical Applications • What are the differences between (advantages of) synthetic and natural phospholipids? • What are the advantages/disadvantages of including cholesterol in my liposomes? • How do I get my hydrophobic drug in the lipid membrane? • What issues should I consi...

California Cleaning Product Right to Know Act ‒ Global Implications

With a population of almost 40 million and a Gross State Product (GSP) of $2.75 trillion (€2.43 trillion), the state of California is, by far, the largest market in the USA. Because its economy is larger than most countries (outside of the US, only China, Japan, and Germany have larger economies), r...

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

Nature Protocols Volume 2 Issue 9, 13 September 2007, Pages 2212 - 2221 doi: 10.1038/nprot.2007.321 Nature Publishing Group Frank H Niesen a , Helena Berglund b , Masoud Vedadi c a Structural Genomics Consortium, Botnar Research Centre, Oxford University b Structural Genomics Consortium, Karolinska ...

Sample components that can interfere with LC-MS results

Purpose of sample preparation prior to LC-MS is to bring sample to a form that is suitable for chromatography (Dissolved in a solvent & particle free), but sometimes depending on the analytes and matrix components complex sample preparation is required to be performed on these samples. The reason fo...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Plant RNA/DNA Purification Kit

Norgen’s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants. The total RNA and DNA (including genomic DNA) and are both column purified in under 30 minutes using a single column. It is often nec...

HIS-Select Convenient Formats

• HIS-Select® iLAP® 5 ml Column • HIS-Select® High Flow Cartridges • HIS-Select® Cartridge • HIS-Select® Spin Column • EZView™ Red HIS-Select® Nickel Affinity Gel HIS-Select® iLAP® 5 ml Column, (H9913) The HIS-Select iLAP (Integrated Lysis and Affinity Purification) 5 ml Column is a single-use, disp...

Simple, efficient extraction of protein from hepatocytes with CytoBusterTM Reagent

Cytochromes p450 (CYP) are a large group of heme-containing mono-oxygenases which catalyse many important biological functions (1). These most notably include the oxidative conversions of many steroids, lipids, environmental toxins and xenobiotics. This family of enzymes plays a significant role in ...

Troubleshooting 2-D DIGE Results

Table 44 lists problems that may be encountered in 2-D electrophoresis results, describes the possible causes, and suggests ways to prevent problems in future experiments. For troubleshooting problems encountered during the various steps of the 2-D process, refer to the following: • Table 20, page 7...

carnitine palmitoyltransferase 1A (liver)
Synonyms CPT1, CPT1-L, L-CPT1
Species Human (1374) , Species Mouse (12894) , Species Rat (25757) , Species Zebrafish (558088) , Species cow (506812) , Species Horse (100009716) , Species domestic cat (101096105) , Species dog (403583) , Species chicken (423118) , Species sheep (443434) , Species domestic guinea pig (100728420) , Species naked mole-rat (101716055)
Summary: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Working with BRAND® Liquid Handling Instruments

The ever increasing demands on the quality of analytical results, and the growing number of samples to be processed result in a need for volumetric instruments which help to handle the routine work in sample preparation as efficiently as possible. Manufacturers of laboratory equipment have responded...

Agricultural Contaminants - Pesticide Classes & Exposure Hazards

(back to Agricultural Contaminants) Most pesticides contain an active ingredient or agent, synergists, solvents for dispersion and stabilization and adjuvants. Synergists enhance the killing power of the chemical agent. Adjuvants are additional additives, which may give many pesticides their competi...

Tricine–SDS-PAGE

Nature Protocols Volume 1 Issue 1, 12 June 2006, Pages 16 - 22 doi: 10.1038/nprot.2006.4 Nature Publishing Group Hermann Schägger a a Molekulare Bioenergetik, Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 26 Abstract Tricine–SDS-PAGE is commonly used to s...

Sample Purification & Quality Assessment - PCR Technologies Guide

• Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR qPCR dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Product No. 11719386001 Cell lysis and sample preparation 1. Wash cells/tissue at least twice with ice-cold PBS to remove any remaining serum proteins from the culture medium. For one immunoprecipitation reaction a sample volume of 1 to 3 ml is recommended. Using a microcentrifuge, a volume of 1 ml ...

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media. Problem Possible cause Solution GST-tagged protein does not bind or binds poorly to the medium. The flow rate used during sample loading is t...

BioUltra Reagents Detergents & Surfactants

Introduction Structures of common surfactants Analytical Information Applications References Alphabetical List of Products Introduction Surfactants constitute the most important group of detergent components. Generally, these are water-soluble surface-active agents comprised of a hydrophobic portion...

HIS-Select® iLAP®

Screening a large number of recombinant clones for the production of soluble target proteins can be time consuming and laborious. Sigma’s patented HIS-Select® iLAP® (Integrated Lysis and Affinity Purification) column and plate technology meets the need of researchers who screen large numbers of solu...

Sample Preparation in Ion Exchange Chromatography

Appendix 1, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007 Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, may reduce the need...

Purification or Removal of NADP+ Dependent Dehydrogenases and Other Enzymes with Affinity for NADP+

Extracted from Affinity Chromatography Vol. 3: Specific Groups of Biomolecules, GE Healthcare, 2014 NADP+-dependent dehydrogenases interact strongly with 2’5’ ADP. Selective elution with gradients of NAD+ or NADP+ has allowed the resolution of complex mixtures of dehydrogenase isoenzymes using 2’5’ AD...

Western Blot Protocol | Immunoblotting Protocol

Related Links • Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Western Blotting Home Page • Western Blotting Literature & Resources • Antibody Explorer: Search primary and secondary antibodies Western ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

The past is a source of knowledge, and the future is a source of hope. S. Ambrose (1936-2002) Preserved samples from medical, forensic, museum and other archival collections represent a rich source of study material, much of it meticulously collected, characterized and preserved through many decades...

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MSpngase fast asms

• We have developed a proprietary detergent-based buffer for rapid deglycosylation of N-linked glycans using PNGase F. • Complete release of N-glycans is achieved in 15 minute incubation vs typical overnight digestions. • Substrate reduction before PNGase F treatment was evaluated as an optional vs ...

Salt Types and Additives in Multimodal Chromatography Media

Extracted from Multimodal Chromatography (PDF), GE Healthcare, 2013 Different salt types and additives can modulate the interactions of target molecule with multimodal chromatography media. Because hydrophobic interaction is one of the interaction modes that is often involved, the choice of salt may...

Enzyme Reagents - Proteases for Mitochondrial Isolation

Mitochondria isolation is commonly utilized for apoptosis studies.1 Such studies are of central importance for the investigation of a number of major debilitating diseases including Parkinson’s disease and cancer.2,3 In addition, mitochondrial protein isolation is of importance in proteome studies.4...

Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells

The baculovirus expression vector system (BEVS) has been widely used for high-level expression of eukaryotic genes in insect cells.1,2 Cultured insect cells are capable of producing 100 mg target protein per 109 cells with baculovirus vectors expressing target genes under the control of AcNPV polh o...

Mesoporous Silica and their Applications

Since their discovery in the late 1970s, mesoporous silica have attracted quite a bit of attention. This is mainly due to their unique properties such as ordered pore structures, very high specific surface areas and their possible synthesis in a wide range of morphologies such as spheres rods, discs...

Downstream Applications after Genomic DNA Purification

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 The following table summarizes the general quantity and purity considerations for several common applications for genomic DNA. Table 4.3. Summary of downstream applications for genomic DNA and substances that...

Nanodisc Technology: A Revolutionary System for Study of Membrane Proteins

Membrane proteins are central to biological signaling pathways, regulating transfer of information and energy across cell membranes. They compose roughly 30% of the human proteome, but due to their key roles in signaling control, represent as much as 50% of therapeutic drug targets. Functionality of...

Enzyme Explorer Product Application Index for Elastase

On this page: • Physical Properties • Clinical Implications • Specificity, Kinetics and Activity Determination • Storage and Stability • Related Products Enzyme Commission (EC) Number: 3.4.21.37 Product Number: E8140 Synonyms: lysosomal elastase, neutrophil elastase, polymorphonuclear leukocyte elas...

Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots

This calculator enables the accurate preparation of this Western blot blocking solution whether you are making enough for a single experiment or multiple liters for the entire lab. Input your desired concentration and volume and click the CALCULATE button. The table will populate with the amounts of...