Area of Interest
Miscellaneous(113)
Technical Documents(196)
Application
2D Electrophoresis(1)
Adrenergic agents(2)
Affinity chromatography(16)
AGE(43)
Agglutination(2)
Amino acid analysis(6)
Amplification(80)
Analgesics(2)
Anti-depressants(2)
Anti-inflammatory agents(13)
Antibiotics(18)
Anticoagulants(5)
Anticonvulsants(4)
Antifungals(4)
Antihypertensives(5)
Antimicrobials(9)
Antipyretics(1)
Antitumor agents(6)
Antivirals(7)
Asymmetric synthesis(6)
Atomic absorption spectroscopy(25)
Atomic emission spectroscopy(5)
BCA assay(4)
Bioactive small molecules(1)
Bradford assay(2)
Buffers(58)
Capillary electrophoresis(11)
Capture ELISA(3)
Carcinogens(5)
Cell biology(8)
Cell culture(31)
Cell disruption(52)
Cell dissociation(5)
Centrifugation(49)
Characterizations(2)
Chiral chromatography(2)
Chiral resolution(3)
Chromatin immunoprecipitation(18)
Chromatography(55)
Cloning(56)
Column chromatography(4)
Cryopreservation(1)
Culture media(3)
Derivatizations(5)
Detection methods(10)
Detergents(36)
Detoxification(2)
Diagnostic Imaging(1)
Digestions(314)
Direct immunofluorescence(1)
Displacement chromatography(2)
Diuretics(2)
DNA microarrays(1)
DNA purification(26)
DNA replication(6)
Dopamine agents(1)
Electroblotting(1)
Electron capture detector(1)
Electron spin resonance(1)
Electrophoresis(72)
Elemental analysis(9)
Emission spectroscopy(5)
Enzyme activity(17)
Enzyme-linked immunosorbent assay(14)
Extinction coefficient(9)
Fast protein liquid chromatography(1)
Flame ionization detector(3)
Flow cytometry(6)
Fluorescent microscopy(1)
Fractionation(13)
Gas chromatography(26)
Gas chromatography mass spectrometry(2)
Gel electrophoresis(52)
Gene knockdown(5)
Growth factors(18)
Hemostatics(1)
Herbicides(5)
High performance liquid chromatography(65)
Homogenization(19)
Hormones(11)
Immobilization(25)
Immunoassay(3)
Immunocytochemistry(4)
Immunofluorescence(4)
Immunohistochemistry(4)
Immunoprecipitation(19)
In Situ hybridization(4)
Indicators(7)
Indirect Immunofluorescense(2)
Individual protein Immunoprecipitation(8)
Inductively coupled plasma(17)
Infrared spectroscopy(3)
Insecticides(1)
Ion-exchange chromatography(3)
Isoelectric focusing(6)
Kjeldahl titrations(2)
Lab-on-a-chip(1)
Ligands(11)
Liquid chromatography mass spectrometry(27)
Lowry assay(1)
Mass selective detector(1)
Mass spectrometry(71)
Metabolites(17)
Microarray Analysis(20)
Microscopy(9)
Microwave synthesis(3)
Molecular biology techniques(4)
Molecular probes(5)
Mutagens(3)
Neurotransmitters(3)
Normal-phase chromatography(1)
Northern blot(3)
Nuclear magnetic resonance spectroscopy(6)
Nucleic acid annealing(22)
Nucleic acid denaturation(32)
Nucleic acid hybridization(25)
Nucleic acid purification(7)
Oligonucleotide microarrays(1)
PAGE(76)
Pesticides(3)
Pharmacological actions(3)
Polymerase chain reaction(114)
Polymerase chain reaction - quantitative(36)
Positron Emission Tomography(4)
Prebiotics(4)
Probiotics(4)
Protein assay(7)
Protein electrophoresis(1)
Protein extraction(10)
Pulsed field gel electrophoresis(4)
Purification(129)
Resolving agents(3)
Reversed-phase chromatography(4)
RNA immunoprecipitation(4)
RNA purification(13)
Sample preparations(45)
Scanning electron microscopy(6)
Scintillation(1)
SDS PAGE(3)
Separation(41)
Sequencing(76)
Simulated moving-bed chromatography(2)
Size-exclusion chromatography(19)
Solid phase extractions(13)
Solid phase microextractions(3)
Solvents(23)
Southern blot(1)
Spectroscopy(16)
Sterilizations(3)
Supercritical fluid chromatography(4)
Tagged proteins(4)
Teratogens(1)
Thin layer chromatography(7)
Tissue microarrays(1)
Titrations(8)
Transduction(7)
Transfection(31)
Transmission electron microscopy(1)
Ultraviolet-Visible spectroscopy(1)
Vitamins(12)
Western blot(22)
Whole genome amplification(8)
X-Ray diffraction(2)
Disease
Alzheimer Disease(4)
Cancer(39)
Cardiovascular(10)
Central Nervous System(4)
Dermatology(1)
Diabetes(12)
Diseases(17)
Gastrointestinal(9)
Huntington Disease(1)
Infectious Diseases(2)
Industry
Agriculture(5)
Allergens(1)
Analytical Chemistry(2)
Bio-Analytical(1)
Chemical industry(1)
Clinical(37)
Clinical Chemistry(5)
Cosmetics(5)
Diagnostic(18)
Environmental(13)
Forensic(5)
Industries(3)
Inorganic trace analysis(1)
Petrochemical(1)
Pharmaceutical(17)
Semiconductor(1)
Veterinary(1)
Process / Function
Absorption(44)
Acetylations(7)
Acylations(3)
Addition reactions(6)
Adhesion(14)
Adsorption(19)
Aerobic(2)
Alkylations(10)
Amidations(1)
Aminations(1)
Anaerobic(3)
Angiogenesis(9)
Apoptosis(22)
Arylations(1)
Autophagy(2)
Bacterial conjugations(2)
Biological processes(2)
Biotinylations(2)
C-mannosylation(1)
Calcification(1)
Carbohydrate metabolism(1)
Carboxylations(2)
Catalysis(22)
Cell division(2)
Cell proliferation(14)
Cell signaling(7)
Cellular processes(6)
Chemical vapor deposition(1)
Coagulation(11)
Combustion(1)
Condensations(2)
Coupling reactions(3)
Crystallization(6)
Deamidations(1)
Deaminations(1)
Degradations(45)
Dehydration reaction(4)
Deposition(4)
Desiccation(1)
Dialysis(8)
Diffraction(2)
Diffusion(7)
Dihydroxylations(1)
Eliminations(17)
Epimerizations(1)
Epoxidations(2)
Evaporation(10)
Fermentation(8)
Filtration(34)
Fucosylation(1)
Gene expression(81)
Genetic(27)
Germination(1)
Glycosylations(17)
Grignard Reaction(1)
Hydration reaction(4)
Hydrogenations(1)
Ion Exchange(9)
Isomerizations(2)
Lipid Metabolism(5)
Lipid peroxidation(6)
Lipid signaling(1)
Lithiations(1)
Melting(13)
Metabolic Pathways(2)
Metabolism(26)
Methylations(15)
Morphogenesis(1)
N-linked glycosylation(3)
Neurotransmission(1)
Oxidations(17)
Palmitoylations(2)
PEGylations(1)
Peptide synthesis(35)
Peroxidations(6)
Phase transitions(31)
Phosphorylations(22)
Physiological Processes(4)
Polymerization reactions(6)
Polymorphisms(15)
Post translational modifications(5)
Precipitation(57)
Rearrangements(4)
Recombination(11)
Reductions(41)
Reductive aminations(1)
Reproduction(3)
Respiration(1)
Sharpless Dihydroxylation(1)
Solid state metathesis(1)
Solvation(2)
Sonication(17)
Substitutions(6)
Sulfations(1)
Transcription(33)
transformation(26)
Tropism(1)
Ubiquitinations(1)
Research Area
Biochemistry(13)
Ceramics(1)
Chemical biology(1)
Chemistry research(2)
Drug discovery(6)
Electronics(1)
Endocrinology(2)
Enzymology(5)
Epigenetics(4)
Functional genomics(4)
Genetics(12)
Genomics(14)
Hematology(2)
Immunology(7)
Medicinal chemistry(5)
Metabolomics(1)
Microbiology(8)
Molecular biology(46)
Nanomaterials(1)
Nanotechnology(2)
Neurology(2)
Neuroscience(4)
Nutrition(7)
Oncology(4)
Plant biotechnology(1)
Printed electronics(1)
Proteomics(40)
Stem cell biology(2)
Surface Science(2)

Available for Sale

Search term:"digestions"

Compare Products: Select up to 4 products.

*Please select more than one item to compare

314 matches found for digestions

Psyllium (Plantago ovata) | Plant Profiler

Synonyms / Common Names / Related Terms Arabinose, Bran Buds® cereal, bulk laxative, Effersyllium®, fiber, Fiberall®, Fiber-loop cereal, flea seed, fleawort, Fybogel®, Heartwise® cereal, hemicellulose, Hydrocil®, Indian plantago seed, isabgol, I-so-gel®, ispaggol, ispaghula, ispaghula husk, ispaghul...

Clone and Downstream Analysis - MISSION shRNA FAQs

What restriction enzymes are recommended for diagnostic digests of the clone DNA? We recommend Pvu II. Restriction digest with Pvu II results in three bands; however, clones with an additional Pvu II site in the hairpin insert will produce four bands: 2513 bp, 2325 bp, 1478 bp and 776 bp. The band s...

A Universal Dual Peptide Assay for Antibody-Based Drug Candidates in Animal PK Studies Using a Full-Length Stable Isotope Labeled Internal Standard

There is a growing demand for reliable LC–MS/MS assays to support the bioanalysis of a human monoclonal antibody-based drug candidates in preclinical studies. Traditional LC-MS/MS assays rely on analyte specific stable isotope labeled (SIL) peptides that provide poor absolute quantitation due to lat...

Restriction Enzyme Double Digest Buffer Guide

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When using two restriction enzymes at once, first check the enzyme activities in each buffer, using table on of the Restriction Enzyme Buffer Reference. If they both have 100% activity in the same buffer, y...

Characterization of Stable Isotope Labeled APO-A1 for Use as an Internal Standard in a Quantitative MS Workflow

Characterize heavy SIL-APOA1 (SILu™ Prot APOA1 Apolipoprotein A-I) for use in quantitative MS. The ideal internal standard for quantitative LC/MS assays would be a stable-isotope-labeled full-length protein that is equivalent to the native target protein which can be introduced at the beginning of t...

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more or less like a Y. Basic structure of the Ig monomer (Figure 1) consists of two identical halves conne...

Avanti® FAQ

Drug Delivery / Pharmaceutical Applications • What are the differences between (advantages of) synthetic and natural phospholipids? • What are the advantages/disadvantages of including cholesterol in my liposomes? • How do I get my hydrophobic drug in the lipid membrane? • What issues should I consi...

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to inhibit the activity of lambda phage by the enzymatic cleavage of the phage DNA was studied and the e...

Restriction Enzymes

Click here to return to the Molecular Biology Guide Restriction endonucleases popularly referred to as restriction enzymes, are ubiquitously present in prokaryotes. The function of restriction endonucleases is mainly protection against foreign genetic material especially against bacteriophage DNA. T...

digestive organ expansion factor homolog (zebrafish)
Synonyms C1orf107, DEF, DJ434O14.5, UTP25
Species Human (27042) , Species Mouse (215193) , Species Rat (305076) , Species sheep (101104924) , Species naked mole-rat (101711120) , Species domestic guinea pig (100730009) , Species Zebrafish (286787) , Species cow (510385) , Species domestic cat (101093911) , Species dog (490274) , Species chicken (421384)

Plasmid DNA purification kits by Sigma Aldrich

Extraction of macromolecules such as DNA, RNA, and protein is one of the basic methods used in molecular biology. The process of extraction and purification of nucleic acids has evolved from being a complex, prolonged, and labor-intensive procedure. Nucleic acid purification technologies now give hi...

Agarose and Acrylamide product lines for nucleic electrophoresis

Agarose Learning Center Agarose Pre-cast Agarose Gels Acrylamide Agarose We offer a range of molecular biology grade agarose products from routine use to wide range and low melt point agarose. Each lot of products is rigorously tested to ensure reproducibility from one lot to the next. Use the follo...

DNA & RNA Purification Five-Minute Miniprep Kit

Description: The GenElute™ Five-Minute Miniprep Kit features an ultra streamlined protocol yielding up to 5 µg high-quality plasmid DNA in about five minutes. • Sophisticated – no pelleting cells or clearing lysates • Flexible – binding column works with any standard laboratory vacuum manifold • Ple...

Plasmid Product Nomenclature

The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. We currently have hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of plasmids. This page contains a ...

Experienced User Protocol GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page. 1. Release DNA · Enzymatic digestion. 2. Prepare Column · Add solution and spin. 3. Bind DNA · Add ethanol and spin. 4. Wash Column · Was...

x-tracta Gel Extraction Tool

The x-tracta gel extraction tool is an easy to use, quick alternative to using scalpel or razor blades for the extraction of DNA and RNA bands from agarose gels following gel electrophoresis. The cutting blade and ergonomic design mean that extractions can be performed in a rapid, easy to follow pro...

Development of Simple and Rapid Workflows for Quantitation of Infliximab and Adalimumab in Human Serum by LC-MS/MS

There is a growing demand for reliable LC-MS/MS assays to support quantitation of serum levels of infliximab and adalimumab monoclonal antibodies, as clinical responses differ between patients due to varying pharmacokinetics and the formation of autoantibodies. We have optimized a simple “pellet dig...

Sample Purification & Quality Assessment - PCR Technologies Guide

• Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR qPCR dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

Cloning the Gene-of-Interest into a Plasmid Vector

Click here to return to the Cloning Manual. • Selecting the Cloning System and Plasmid Vector • Plasmid Restriction Digestion • Fragment Restriction Digestion • Gel Excission • Clean-up of Gel Fragments • Annealing DNA Oligos for Ligation • Adding 5’ Phosphates to DNA • Dephosphorylating DNA • Prepa...

DIG RNA Labeling Mix Protocol & Troubleshooting

Product No. 11277073910 Evaluation of DIG RNA labeling efficiency: Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction). Roche recommends loading an aliquot on ...

Secondary Antibodies, Conjugates and Kits

Related Links • Antibody Explorer • How to Choose a Secondary Antibody Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purifi...

Additional Products

• Main Page • Purification & Enrichment • Characterization & Detection • Protein Deglycosylation Featured Products • Additional Products for Glycoprotein Analysis • Additional Products for Phosphoprotein Analysis • Trypsin In-Gel Digest Kit • Phosphatase Inhibitor Cocktails • Chart of mass changes r...

Genome engineering using the CRISPR-Cas9 system

Nature Protocols Volume 8 Issue 11, 24 November 2013, Pages 2281 - 2308 doi: 10.1038/nprot.2013.143 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Sc...

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Related Articles • Reporter US Volume 34.4 • Robustness of the Discovery® HS F5 HPLC Column in the Study of Metabolomics • Reaching the “Peak” of Recovery: Improving Antibody Separations with the Inclusion of Organic Alcohols • Analysis of Human Plasma Lipids Using sub-2 μm C18 UHPLC Column with MS ...

Proteomics & Protein Expression Poster Gallery

Featured Poster Use of an Animal-free Synthetic Surrogate Serum Matrix for Preparation of Calibrators and Controls in Immuno- and LC-MS Based Clinical Assays Click on the quick links below to see posters on each topic • Mass Spectrometry Tools • ProteoPrep™ Plasma Immunodepletion • NEW! SILu™Prot Ma...

OSHA 1006– Products and Materials Guide

Arsenic, Cadmium, Cobalt, Copper, Lead, and Nickel (Open Vessel Microwave Digestion/ICP-MS Analysis) This method describes the collection and subsequent analysis of airborne metal and metalloid particulates by ICP-MS. It provides rapid preparation of samples collected on MCE filters, and simultaneou...

Astec Chiral Chromatography Bibliography by Subject

Amino Acids, Peptides, Biomolecules Chemical, Environmental, Petrochemical Chiral LC-MS Food, Beverage, Natural Products Mechanistic Studies Pharmaceutical, Clinical Preparative, SFC, SMB Daniel W. Armstrong Publications Copyright restrictions do not permit us to provide copies of the articles liste...

Neural Stem Cell FAQs

The use of neural stem cells (NSCs) in biomedical research is becoming increasingly popular, resulting in breakthrough studies rejected the longstanding belief that neuronal tissue is incapable of regeneration. The discovery that neurons, astrocytes, and oligodendrocytes arise from neural stem cells...

Tag Removal by Enzymatic Cleavage

Related Articles • Affinity Chromatography in a Purification Strategy (CIPP) • Characteristics of Dextrin Sepharose® High Performance Products • Characteristics of Glutathione Sepharose ® Products • Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose®...

New Enzymes for Antibody Characterization

• Antibodies now make up the fastest growing category of therapeutic drugs. Even with today's highly-defined recombinant humanized antibodies, the control and analysis of micro-heterogeneities relating to functional impact have become critical to the quality by design paradigm.1 Alain Beck and his c...

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Our GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, a...