Area of Interest
Product Directory(1)
Technical Documents(6)
Application
Amplification(1)
Buffers(4)
Cell culture(2)
Cell disruption(6)
Centrifugation(3)
Chromatin immunoprecipitation(3)
Culture media(1)
Detergents(3)
DNA purification(1)
Electrophoresis(2)
Enzyme activity(2)
Gel electrophoresis(2)
Homogenization(3)
Immunoprecipitation(5)
Individual protein Immunoprecipitation(1)
PAGE(5)
Polymerase chain reaction(1)
Polymerase chain reaction - quantitative(1)
Purification(1)
Sample preparations(3)
Solvents(1)
Western blot(2)
Process / Function
Adsorption(2)
Degradations(2)
Evaporation(1)
Precipitation(2)
Sonication(2)

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7 matches found for dounce homogenizer

Immunoprecipitation Kit (Protein G) Protocol & Troubleshooting

Product No. 11719386001 Cell lysis and sample preparation 1. Wash cells/tissue at least twice with ice-cold PBS to remove any remaining serum proteins from the culture medium. For one immunoprecipitation reaction a sample volume of 1 to 3 ml is recommended. Using a microcentrifuge, a volume of 1 ml ...

Immunoprecipitation Kit (Protein A) Protocol & Troubleshooting

Product No. 11719394001 Cell lysis and sample preparation 1. Wash cells/tissue at least twice with ice-cold PBS to remove any remaining serum proteins from the culture medium. For one immunoprecipitation reaction a sample volume of 1 to 3 ml is recommended. Using a microcentrifuge, a volume of 1 ml ...

ChIP-X: Cross-linking proteins to DNA and cell lysis

Cross-linking stabilizes the association of your target protein with its interacting DNA sequences. In some cases, target proteins are already tightly associated with DNA and additional chemical cross-links are not required to preserve the protein/DNA complex during the experimental analysis. This i...

ChIP Troubleshooting Tips

Problems Suggested Solutions High background in negative control (IgG or mock IP) samples Excessive antibody resulting in binding to non-targets: Optimize the concentration of the antibody. Nonspecific binding to beads: Include a pre-clearing step to exclude these non-targets or add a blocking agent...

ChIP Supplementary Protocols

1. If necessary, stimulate or treat adherent mammalian cells at ~80 to 90% confluence in a 150 mm culture dish containing 20 mL of growth media. Include one extra plate of cells to be used solely for estimation of cell number. 2. Prepare 22 mL of 1X PBS (2.2 mL 10X PBS and 19.8 mL water) for each 15...

Tissue Homogenizers - Labware

A homogenizer is a common piece of equipment used in laboratory or industrial applications for the homogenization of soil, plant tissue, food and other substances. They are required for sample preparation prior to the analysis of cells, proteins, nucleic acids, and many other biological samples. Dou...

TriPure Isolation Reagent Protocol

Product No. TRIPURE-RO Isolation of nucleic acids and proteins from plant sample material, e.g. leafs Roche has not tested this experimental protocol that has reportedly worked successfully for at least Dandelion and Philodendron samples. In general, yields are better for plants having thin leaf wal...