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171 matches found for enzyme_activity

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate. The Spectrophotometric Stop Rate Determination [Absorbance at 340 nm (A340), Light path = 1 cm] is based on the following reactions: Amyloglucosidase Starch + water ––––––––––––––––> D-Glucos...

Enzyme Explorer Lectin Index

We have the largest selection of lectins and lectin conjugates available, with over 100 products from over 35 sources. Backed by quality and service you’ve come to expect, we offer lectins for all your life science applications, including: • Histochemical Studies • Lymphocyte Subpopulations Studies ...

In Pursuit of the Elements

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Based on SSSTAR02. Refer to CR SOP DEK ENZ 62. To standardize a procedure for determining the enzymatic activity of β-Amylase. This procedure applies to all products that have a specification for β-Amylase. 3.1 Purified Water - water from a deionizing system, resistivity > or = 18MΩ•cm @ 25ºC 3.2. U...

Metabolomics

Comprehensive Metabolomic resources, reagents and kits. We offer a large collection of Amino Acid Metabolites and Carbohydrate Metabolites. We carry enzymatic-based kits for the quantitation of nutrients and metabolites. Our alliance with the IUBMB enables us to feature the Nicholson Metabolic Pathw...

Strategy for Toxicity Screening of Nanomaterials - Material Matters Volume 5 Issue 3

• • Download PDF • 1Division of NanoMedicine, Department of Medicine, University of California, Los Angeles, CA 90095 2California NanoSystems Institute, University of California, Los Angeles, CA 90095 3Department of Chemistry & Biochemistry, University of California, Los Angeles, CA 90095 *Email: an...

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes

Product lists for commonly used protocols from Cold Spring Harbor Protocols Thomas Lufkin This protocol was adapted from "Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems," Chapter 16, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kris...

Ammonium Sulfate Suspensions - Technical Note

— If the ammonium sulfate might interfere with the reaction(s): 1. Remove the enzyme from the refrigerator. Note: We strongly recommend that ammonium sulfate suspensions not be frozen. 2. Make sure the cap is on tight, and then invert the vial/bottle several times to provide a milky suspension. This...

Bio-Inspired “Green” Synthesis of Nanomaterials and their Applications

Dr. Siddharth V. Patwardhan Department of Chemical and Process Engineering University of Strathclyde, 75 Montrose Street Glasgow G1 1XJ, UK Email: Siddharth.Patwardhan@strath.ac.uk Silica is a very popular inorganic nanomaterial used in a wide range of applications including fillers for rubber, cata...

ubiquitin-like modifier activating enzyme 2
Synonyms ARX, SAE2
Species Human (10054) , Species Mouse (50995) , Species Rat (308508) , Species cow (507005) , Species sheep (101112668) , Species domestic guinea pig (100719212) , Species Domestic Rabbit (100355779) , Species naked mole-rat (101719788) , Species fruit fly (44496) , Species Horse (100057234) , Species domestic cat (101089562) , Species dog (476490) , Species chicken (415784) , Species Zebrafish (406672)
Summary: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]

Characteristic Metabolites for Inborn Errors of Lipid Metabolism

BioFiles Volume 5, Number 7 — Lipid Metabolism Download BioFiles v5 n7 (PDF) Table of Contents • Introduction • Natural Abundance Lipid Metabolites Glycerolipid Metabolsim Glycerophospholipid Metabolism Ether Lipid Metabolism Spingolipid Metabolism Fatty Acid Metabolism Terpenoid Metabolism Steroid ...

Enzymatic Deglycosylation

Overview N-Linked Glycan Strategies Native N-Linked Strategies O-Linked Strategies Endoglycosidase Specificities Kits for Enzymatic Deglycosylation References Overview Removal of carbohydrates from glycoproteins is useful for a number of reasons: • To simplify analysis of the peptide portion of the ...

BioUltra Reagents Density Gradient

Introduction References Product List To meet highest demands for quality, we offer a wide range of BioUltra Reagents for density gradient centrifugation. Introduction Criteria for an ideal density gradient centrifugation medium are: ○ the additive must form a solution within the required density ran...

RNA stabilization and protein stabilization in tissue extracts with Stabilyser™ Reagent

Related Products • Protein Sample Prep • Western Blotting • Proteomics Maintain nucleic acids and functional proteins with Stabilyser™ reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture. Now, you can stabilize complete, uniform tiss...

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275). This ratio (A403/A275) is not a measure of enzyme activity. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P8125, P8170, P8250, P8375, P8415, ...

Working with PCR

Sign-up to receive the Genomics Express eNewsletter. Subscribe now! • General Considerations ○ Setting up the laboratory to avoid contamination ○ Special equipment ○ PCR reaction components ○ Thermal cycling profile for standard PCR ○ Properties of PCR enzymes • Factors to Consider in RT-PCR ○ Choic...

Applications of Y-Shape Peg Derivatives for Drug Delivery

JenKem Technology Co. Ltd., Beijing, P.R. China Email: tech@jenkemusa.com The immune system protects the body from disease by resisting the invasion of foreign molecules into its cells, for example peptides and proteins are hydrolyzed by proteolytic enzymes; nucleic acids are hydrolyzed by nucleases...

Application Index: Chymase

Recent discoveries have implicated chymase to be a key mediator in cardiovascular and inflammatory cell signaling pathways. Sigma now offers recombinant human Chymase expressed in Pichia pastoris Applications in: • Angiotensin II Generation • Big Endothelin Conversion • ECM Degradation • NO Producti...

Liberase™ Research Grade Selection Kit Protocol & Troubleshooting

Product No. 05401046001 Selection of the appropriate Liberase Research Grade Enzyme Blend Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend. Select the Liberase ...

Analytical Enzymes - Proteinase K

E.C. 3.4.21.64 Synonym: Endoproteinase K On this page: • Products and Manufacturing Capabilities • Specificity • Physical Properties and Kinetics • Inhibitors • Applications • Solubility and Solution Stability This protein is available in the BioUltra Grade. Learn more here. Proteinase K exhibits br...

Growth Regulators - Plant Tissue Culture Protocol

• Preparation and Use • Table for Molar Equivalence and Solution Preparation The importance of plant growth regulators in plant tissue culture is well documented. We offer a broad range of plant growth regulators specifically tested for plant cell culture. Each product is assayed for physical and ch...

DNA Ligation Kit

Product Code: LIG1 Product Description Reagents Provided Precautions and Disclaimer Storage Procedure Materials References One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. This ability to join fragments of DNA through recombinant technology ...

Sigma Receptors

Although they have undergone a substantial transformation from the time they were first proposed in the mid-1970s, a unifying picture appears to be emerging for sigma receptors. Current interest is related to the possibility that sigma receptors may constitute favorable targets for drug design in co...

Universal Protease Activity Assay: Casein as a Substrate [VIDEO]

A. Abstract Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality contro...

Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

This assay protocol is suitable for the colorimetric detection of branched-chain amino acids, leucine, isoleucine, and valine, in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Branched Chain Amino Acid Kit (MAK003). BCAA concentration is determine...

General Considerations for Plasmid DNA Preparation

Extracted from Nucleic Acid Sample Preparation for Downstream Analyses, GE Healthcare, 2007 The yield and purity of isolated plasmid DNA will be influenced not only by the method of isolation, that is, whether a commercially available purification kit is used, but also by a number of external factor...

Arnica (Arnica montana) | Plant Profiler

Synonyms / Common Names / Related Terms 6-methoxykaempferol, Aconitum napellus, alisma, American arnica, Arnica augustifolia, Arnica chamissonis, Arnica cordifolia, arnica da serra, arnica flower, Arnica fulgens, Arnica latifolia, Arnica lonchophylla, Arnica montana, arnica root, Arnica sororia, arn...

Measuring Kinase Inhibitor Residence Times

Introduction This Application Note provides a protocol and experimental example for the use of the Transcreener® ADP2 FP Assay to determine the residence time of a drug (or drug candidate) during its interaction with a kinase. The duration of residence time may be highly relevant for assessing the d...

Casein Blocking Buffer for Southern or Western Blotting

Related Links • Western Blotting Tools Home Page • Introduction to Western Blotting • Western Blot Literature & Resources • Western Blot Reagents • Chemiluminescent Substrates • Western Blot Troubleshooting Guide • Cell Lysis and Protein Extraction Products • Antibody Explorer • Western Blotting Han...

SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantities of double-stranded cDNA are generated from low nanogram to picogram quantities of total RNA in ei...

Enzymes for Cell Detachment and Tissue Dissociation

• • Download PDF • BioFiles 2006, 1.2, 3. Collagenase cleaves the peptide bonds in native, triple-helical collagen. Because of its unique ability to hydrolyze native collagen, it is widely used in isolation of cells from animal tissue. Collagenases occur in a variety of microorganisms and many diffe...

Protein Prenyltransferases

Protein prenylation involves attachment of either the 15-carbon farnesyl or 20-carbon geranylgeranyl isoprenoid to conserved carboxyl-terminal cysteine residues of proteins. Three known enzymes catalyze this type of modification; these are termed protein farnesyltransferase (FTase), protein geranylg...