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382 matches found for filtration

Horsetail (Equisetum arvense) | Plant Profiler

Synonyms / Common Names / Related Terms Bottle brush, cola de caballo, common horsetail, common scouring rush, corncob plant, corn horsetail, Dutch rush, Equisetaceae (family), Equisetum arvense, Equisetum myriochaetum, Equisetum ramosissimum, Equisetum telmateia, field horsetail, horse willow, hipp...

Importance of TFF for concentration

Filtration is a pressure-driven separation process that uses membranes to separate components in a liquid solution or suspension based on their size and charge differences. The two kinds of filtration are tangential flow filtration and normal flow filtration. In Normal Flow Filtration (NFF), fluid i...

Sterile Millex® Filters for Cell Culture

Millex syringe filters are ideal for sterilizing 1 mL – 200 mL of cell culture reagents, such as cell culture media, antibiotics, supplements, sera, viruses for infection, and other biological solutions. Many sterile Millex filters are CE-marked and/or are approved as medical devices, meaning that t...

Biochemical / Biotechnical

Download PDF Product Name PDF file size No. of Pages Subject T111871 Bulletin 871 168 KB 4 Ready-to-Use, Sterile, Endotoxin-Free Adsorbent Cartridges for Purifying Biological Preparations. T195875 Bulletin 875 849 KB 40 Supelco Capillary Column Selection Guide T196898-90 Bulletin 898 (Spanish Versio...

Western Blot Troubleshooting Guide

As your Western blotting partner, our technical support team is ready to help you analyze protein expression and protein-protein interactions with sensitivity and reproducibility. Troubleshoot your Westerns using the reference guide below, download the Protein Blotting Handbook (PDF) for principles ...

Primary Cell Culture

Primary cells maintain physiological relevance because they are derived directly from living tissue and thus find increasing use in life science research and pharmaceutical drug. We are pleased to offer primary cells from Cell Applications Inc. (CAI). CAI has honed the isolation, purification, subcu...

Dot/Slot (Filtration) Blotting: Western Blot Troubleshooting

Related Links • Western Blotting (Immunoblotting) Protocol • 30-minute Immunodetection Protocol Using the SNAP i.d.® 2.0 System • Sample Preparation Methods from GE Healthcare • Products for Cell Lysis and Protein Extraction • Western Blotting Home Page • Western Blotting Literature & Resources • We...

Frequently Asked Questions about Solvents in Hematology & Histology Research

General Information Tax-Paid Ethanol Physical Properties Application Notes Returnable Containers General Information How do I get specific lot information or CofA for a solvent? A Certificate of Analysis is available by lot for each of our solvents and can be accessed through our Advanced Search Opt...

High-throughput Screening Using GST MultiTrap FF and GST MultiTrap 4B 96-well Filter Plates

GST MultiTrap FF and GST MultiTrap 4B (Fig 3.4) are prepacked, disposable 96-well filter plates for reproducible, high-throughput screening of GST-tagged proteins. The plates are filled with a defined amount of affinity medium, Glutathione Sepharose 4 Fast Flow (4% highly cross-linked agarose beads)...

nephrosis 1, congenital, Finnish type (nephrin)
Synonyms CNF, NPHN, nephrin
Species Human (4868) , Species Mouse (54631) , Species Rat (64563) , Species sheep (101115895) , Species naked mole-rat (101705086) , Species Domestic Rabbit (100354996) , Species domestic guinea pig (100713185) , Species Zebrafish (692352) , Species domestic cat (101101018) , Species dog (484571) , Species cow (519706)
Summary: This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Analysis of Mycotoxins in Corn, Wheat, and Peanut

The Supel™ Tox AflaZea SPE cartridge was used to demonstrate the cleanup of corn meal, wheat meal, and raw peanut paste matrices prior to the analysis of Aflatoxin B1, B2, G1, and G2. In the procedure, corn meal, wheat meal, and raw peanut paste extracts were spiked with Aflatoxin Standard Mix (Prod...

GenElute Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. The purified DNA is greater than 20 kb in length and can be used in sensitive downstream applications such as restriction endonuclease digests and PCR amplification. Re...

radiello® Diffusive Air Sampling Applications - Aldehydes

radiello Table of Contents Blue diffusive body Product No. RAD1201 Supporting plate Product No. RAD121 Vertical adapter Product No. RAD122 (optional) Chemiadsorbing cartridge Product No. RAD165 Filtration kit Product No. RAD174 (only for analysis) Or: radiello-ready-to-use sampler Product No. RAD123...

HIC for Intermediate Purification

The objective of intermediate purification steps is to remove most of the significant impurities such as proteins, nucleic acids, endotoxins and viruses. In a typical intermediate purification step, speed is less critical since sample volume has been reduced and damaging contaminants have been remov...

Cell Culture

As people, we hope for longer, healthier and more productive lives. As scientists, we work every day to make that hope a reality. For cell culture, that means creating a consistent environment to ensure validity of results. Our comprehensive portfolio of high quality products includes everything fro...

CBILS©

Aldrich ChemFiles 2006, 6.9, 8. A Revolutionary Tool for Ionic Liquid Synthesis Roland St. Kalb and Michael J. Kotschan, proionic Production of Ionic Substances GmbH, Leoben, Austria; www.proionic.at; info@proionic.at “When I first started to work with ionic liquids years ago, it always was an unful...

LPLC Purification Media: Custom Solutions to Your Unique Challenges

Sigma-Aldrich offers a broad media portfolio supported by custom services that optimize your purification results Lori Fields It has been said that the only thing that all purification applications have in common is that they are all unique. Most users share the common desire to obtain purified mate...

Sample Purification & Quality Assessment - PCR Technologies Guide

• Introduction and Historical Timelines • Polymerase Chain Reaction • Quantitative PCR • Digital PCR • Quantitative PCR and Digital PCR Detection Methods • PCR qPCR dPCR Assay Design • Sample Purification and Quality Assessment • Reverse Transcription • Assay Optimization and Validation • Data Analy...

Graphene Quantum Dots: Properties, Synthesis & Applications

Colloidal semiconductor quantum dots (QDs) have numerous potential applications in solar cells, light emitting diodes, bioimaging, electronic displays, and other optoelectronic devices due to their unique size-dependent electro-optical properties, and have thus been of significant research interest....

Selected Applications of Metal-Organic Frameworks in Sustainable Energy Technologies

• • Download PDF • Stephen R. Caskey Adam J. Matzger Stephen R. Caskey1 and Adam J. Matzger2 1Sigma-Aldrich Corp., Milwaukee, WI 53209 2Department of Chemistry and the Macromolecular Science and Engineering Program, University of Michigan, Ann Arbor, MI 48109 Email: stephen.caskey@sial.com, matzger@...

Mass Spectrometry of Glycans | BioFiles Volume 5, Number 1 Glycobiology

BioFiles Volume 5, Number 1 — Glycobiology Download Biofiles v5 n1 (4.4 Mb PDF) Table of Contents • Introduction • Sialic Acid Synthesis and Signaling • Mass Spectrometry of Glycans • Histological Detection of Pathogenic Fungi in Human Tissue • Select-HA™ • New Products for Glycobiology and Glycoche...

Protein Sample Ultrafiltration

Since our original research on protein concentration using ultrafiltration1, we’ve arrived at perfection. Today’s Amicon® Ultra filters are made with high-quality Ultracel® regenerated cellulose membrane. On this page, you’ll find technical information on the properties of ultrafiltration membranes ...

Assay Procedure for Lipase

The appearance of quinoneimine dye is measured at 545nm by spectrophotometry. One unit causes the formation of one micromole of glycerol (half a micromole of quinoneimine dye) per minute under the conditions described below. Reagents A. Olive oil emulsion :Sonicate the mixture of 5.0g of olive oil[r...

Salt-Free Diorganozincs

Zn(OMe)2 is an exceptional precursor of reactive and selective organozinc reagents under salt-free conditions. Historically, the difficulty in preparing organozinc reagents, their highly pyrophoric nature, and the generation of by-products has decreased their widespread use. The use of additives to ...

Extracellular Vesicle Protocol

Related Links • Extracellular Vesicle Preparation • Extracellular Vesicle Supplementary Protocols • Key Publications and Guidance on Extracellular Vesicle Preparation Enrich for extracellular vesicles in just 40-60 minutes using centrifugal filters. If you are discouraged by ultracentrifugation or p...

Human Albumin

Gene ID: ALB CAS#: 70024-90-7 • Methods of Preparation • Molecular Weight • Structure • Amino Acid Composition • Fatty Acid Composition of Human Albumin • Physical Properties • Applications • Products • References Methods of Preparation: Sigma-Aldrich produces human serum albumin using a cold alcoho...

Selective Extraction and Low Level Quantification of the Mycotoxin Patulin from Organic Apple Puree

Related Products • Supel Tox SPE Products Related Articles • Reporter US Volume 33.2 (4 Mb PDF) • Selective Extraction and Cleanup of Patulin Mycotoxin from Apple Juice Samples • Better Tools for Fast and Convenient Patulin Analysis • Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Mil...

Why Outsource Liquid Solutions?

Greater Simplicity Greater Flexibility Greater Security View the PDF Liquid reagents are growing in importance within bioproduction process development, both upstream and downstream. Market research shows that an increasing number of biologics manufacturers are outsourcing more liquid reagents for t...

Sterility Testing

Sterility testing is an essential part of validation for products manufactured according to GMP purporting to be sterile. False negative results could lead to the release of contaminated products, whereas false positives could give rise to lengthy and costly investigations. Have confidence in your s...

HIC in a Purification Strategy (Cipp)

To ensure efficient, reproducible purification giving the required degree of purity, it is beneficial to develop a multi-step process using the purification strategy of Capture, Intermediate Purification and Polishing (Cipp), shown in Fig 49. Fig 49. Strategy for protein purification. Imagine the pu...

SPE Cleanup of Wheat Extracts Prior to the HPLC Analysis of the Mycotoxin Deoxynivalenol

Deoxynivalenol (DON) is a mycotoxin produced by the Fusarium fungal species graminearum and culmorum. These Fusarium fungi infect cereal crops primarily in wet conditions when temperatures range from 15–30 °C, resulting in grain head damage and DON contamination1. DON is the most common contaminant ...