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145 matches found for flow_cytometry

Biology and Analytical Chemistry Webinar Guide

Biology Webinar Guide Quality Systems and Regulatory Compliance Drug Discovery Webinar Guide Analytical Webinar Guide Materials Science Webinar Guide • Proteomics • qPCR • CRISPR • Cell Culture • Cell Design Studio • Duolink • RNAi • Regulatory Compliance • Late Stage Functionalization of Drug-Like ...

Atto Dyes for Superior Fluorescent Imaging

• Alternatives to common Fluorophores • Reactive Atto Dyes • Atto Dye Conjugates Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridiza...

Peptide Modifications: N-Terminal, Internal, and C-Terminal

Peptide Modification Applications Acetylation Increase peptide stability by preventing N-terminal degradation Biotin Commonly used in immunoassays, histocytochemistry, and fluorescence based flow cytometry Dansyl and 2, 4-Dinitrophenyl Fluorescence based assays Fluorescein and 7-methoxycoumarin acet...

LentiBrite™ for fluorescent cell imaging

Introduction Methods • Construction of lentiviral vectors encoding fluorescent protein fusions • Cell seeding and lentiviral transduction • Live cell imaging and antibody staining • Analysis of GFP-LC3 localization by flow cytometry Results Conclusion Materials Autophagy, a degradative pathway that ...

New M2 Antibody Technical Information Page

New Affinity Purified FLAG® M2 Antibody! Our NEW peptide affinity purified FLAG M2 antibody delivers increased sensitivity and is now supplied in a 50% glycerol solution for even greater stability. We've optimized the purification process to enable sensitive detection of FLAG fusion proteins with a ...

Cell Division Monitoring using Cell Tracking Dyes [VIDEO]

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of different cell types in vitro and in vivo.1-5 Although there are literally thousands of publications using such dyes, some of the most commonly encounter...

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must differentiate into diverse innate and adaptive phenotypes for the body to respond to infections. In t...

Antibodies in Practice: Conjugated and Secondary Antibody Use

Conjugated Antibodies Secondary Antibodies Often for signal amplification and detection purposes, purified antibodies are conjugated to enzymes, fluorophores, or haptens, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), rhodamine, fluorescein isothiocyanate (FITC), or biotin. The var...

Nanostructured Materials Through Ultrasonic Spray Pyrolysis

Related Articles • A Review of Mesoprous TiO2 Thin Film • Synthesis of Melting Gels using Mono-Substituted and Di-Substituted Alkoxysiloxanes • Advanced Inorganic Materials for Solid State Lighting • Materials for Energy Efficiency (4.18 Mb PDF) • Nanoscale Surface Modification (3.24 Mb PDF) • Organ...

PBMC Dendritic Cell Culture Media

Dendritic Cells (DCs) are highly motile immune cells and are the most powerful antigen presenting cells (APCs) of the mammalian immune system.1 DC’s act as messengers between the innate and the adaptive immune systems by continuously sampling their environment for antigens by means of endocytosis.1 ...

Duolink® PLA Custom Services

Duolink® Proximity Ligation Assay (PLA) is a powerful tool that allows in situ detection of endogenous proteins, protein interactions, and protein modifications with high specificity and sensitivity. Although Duolink® PLA experiments are relatively straight-forward, there are many scenarios in which...

Comparing Quantitative Viability Bioassays: An Evaluation of MTT, alamarBlue™, and Guava® ViaCount® Methods

Krista McCutcheon and David Fei, Department of Analytical Sciences, Genentech Inc., South San Francisco, CA 94080-4990 Viability assays are crucial tools in the development of many drugs, particularly of cytotoxic agents. To best characterize potential compounds, viability assays must ideally be abl...

Cancer Research Learning Center

• Bioactive Nutrient Explorer • Cancer Research Techniques • Carcinogen Selector • Comet Assay • Flow Cytometry • Overview of Cancer Biology • Protease Finder • PTK/PTP Expression Profiling • Protocols

Fluorescent Microparticles in Biochemistry

Fluorescently labeled particles are useful as standards in flow cytometry, confocal laser scanning microscopy, and with light scattering instruments. They also have been used in environmental science as tracers in flow measurements of gases and liquids, such as laser Doppler anemometry (LDA), partic...

Hit Discovery & Confirmation for Early Drug Discovery

Hit discovery and confirmation phase helps in the identification of molecules with activity against the target. This phase needs the development of compound screening assays, depending on the drug target. A hit is a compound which has the desired activity in a compound screen. This activity should b...

Neuroscience 2018

We are excited and optimistic about the future of Neuroscience research as we seek to cultivate curiosity, encourage discussion and connect researchers from around the world. Join us at the 2018 Society for Neuroscience Annual meeting to see our newest product innovations we can't wait to share. We ...

At-line monitoring of cell cultures: Rapid cytometric evaluation of cellular physiology

N.T. Mukwena1, F. Veraitch1, S. Santhalingham1, M. Al-Rubeai1 and P. Goix2 1Department of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK 2Guava Technologies, Inc., 25801 Industrial Blvd., Hayward, CA 94545, USA, www.guavatechnologies.com Mammalian cell culture processes face ...

Mesenchymal Stem Cell Characterization

Mesenchymal stem cells (MSCs) are fibroblast-like multipotent adult stem cells derived from multiple tissue (including bone marrow, adipose tissue, umbilical cord, and dental pulp) with the capacity to self-renew. Historically, researchers have isolated these stem cells using various methods and a s...

Laboratory Support and Custom Services

Need an extra pair of hands in the lab? Experimental design can be notably challenging, encountering many unknown and time sensitive paths along the way. Count on us to be those extra pair of hands providing guidance, ideas, green chemistry options and custom consultations. Accelerate your lab perfo...

Lead Discovery for Early Drug Identification

Using the correct tools can help guarantee that after you’ve identified your novel target, you can create the right key to unlock it. We can support you with the innovation you need to identify, evaluate, and rank new lead candidates in a large screen and then confirm them with secondary assays and ...

Duolink® PLA Product Selection Guide

Getting started with Duolink® PLA is simple. The protocols are very similar to that of IF, IHC, and flow cytometry, however Duolink® PLA offers distinct advantages over these and other traditional methods of protein detection. The Duolink® PLA Starter Kit contains all the necessary reagents you need...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

TRC Library Citations

Publish Sooner When You Partner with the MISSION® shRNA Team! The MISSION® TRC1.5 human and mouse shRNA libraries are the proven performers for small-scale to high-throughput RNAi screening. Below you will find a selection of papers where researchers have published in peer-reviewed journals using th...

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recordin...

T Cell Receptor Webinar

T cell biology is integral to the study of normal immune regulation as well as cancer biology, Car-T cells, epitope specificity and antigen presentation. However, primary T cells can be difficult to propagate in culture for the length of time necessary for functional assays. In addition, primary T c...

Key Publications and Guidance on Extracellular Vesicle Preparation

EV preparations can vary in molecular composition and function, depending on: • Cell culture viability • Apoptotic index • Cell proliferation rate • Status of the cell culture medium • Handling conditions • Other experimental parameters To support EV researchers trying to navigate this rapidly chang...

PBMC Macrophage Differentiation

Macrophages are tissue-resident professional phagocytes and antigen-presenting cells (APC), which differentiate from circulating peripheral blood monocytes. They perform important active and regulatory functions in innate as well as adaptive immunity.1 Activated macrophages of different phenotypes a...

Validating the Guava ViaCount® Assay, an Automated Cell Counting/Viability Method for Use in ELISpot Assays

Many of the standard methods currently used for cell counts and viability assessments of cell suspensions, e.g., peripheral blood mononuclear cells (PBMC), do not provide very accurate or reliable measurements. The Guava PCA™ System with the ViaCount assay pro-vides enhanced sensitivity and better p...

Precision Genetic Modifications

Project Types Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address your scientific questi...

Flow Cytometry Webinars

Related Links • Flow Cytometry Instruments • Amnis® Imaging Flow Cytometers • Amnis® Resources • Muse® Cell Analyzer • Guava® Flow Cytometers • CellStream™ Benchtop Flow Cytometers • Applications of imaging flow cytometry for the diagnosis of leukaemia • ToxTracker Assay for High-throughput Screenin...