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146 matches found for flow_cytometry

An Introduction to Antibodies: Antibody-Antigen Interaction

Now that you know what an antigen and antibody are, let us consider the interaction between them. The strength of interaction between antibody and antigen at single antigenic sites can be described by the affinity of the antibody for the antigen. Within each antigenic site, the variable region of th...

Atto Dyes for Superior Fluorescent Imaging

• Alternatives to common Fluorophores • Reactive Atto Dyes • Atto Dye Conjugates Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridiza...

Assessing apoptosis in cell cultures quickly and easily using the Guava® ViaCount® assay

Authors: Kathy Yokobata, Ellen Magallanes, and Colleen Helsel When using the Guava ViaCount reagent and the Guava PCA™ instrument to count cells, cell viability is detected by differential permeation of a DNA intercalating dye. Live cells with intact membranes exclude this stain, while dead and dyin...

Duolink® PLA Applications

With Duolink® PLA, you can detect, quantify, and visualize protein-protein interactions, post-translational modifications, and low expression protein detection with an easy-to-execute immunodetection protocol that is similar to traditional IF or IHC experiments, but yields far more sensitive signals...

How to Optimize the Duolink® Proximity Ligation Assay

Related Products • Duolink® PLA • Primary Antibodies Related Content • Duolink® PLA - Protein Detection Technology • Duolink® PLA Resource Center To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol....

Fluorescent Microparticles in Biochemistry

Fluorescently labeled particles are useful as standards in flow cytometry, confocal laser scanning microscopy, and with light scattering instruments. They also have been used in environmental science as tracers in flow measurements of gases and liquids, such as laser Doppler anemometry (LDA), partic...

Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages

Cellular differentiation, the process by which progenitor cells differentiate into specialized cell types, is fundamental to developmental biology. In the immune system, immune progenitor cells must differentiate into diverse innate and adaptive phenotypes for the body to respond to infections. In t...

Duolink® PLA Flow Cytometry Kits

Traditional flow cytometry has been limited in the ability to detect protein-protein interactions and low abundant proteins events — until now. We have combined Duolink® Proximity Ligation Assay (PLA) with flow cytometry in a convenient kit, making the analysis of protein-protein interactions with f...

Lead Discovery for Early Drug Identification

Using the correct tools can help guarantee that after you’ve identified your novel target, you can create the right key to unlock it. We can support you with the innovation you need to identify, evaluate, and rank new lead candidates in a large screen and then confirm them with secondary assays and ...

Secondary Antibodies, Conjugates and Kits

Related Links • Antibody Explorer • How to Choose a Secondary Antibody Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, purifi...

Protocol, FACS Titration of Lentivirus

FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recordin...

Mesenchymal Stem Cell Characterization

Mesenchymal stem cells (MSCs) are fibroblast-like multipotent adult stem cells derived from multiple tissue (including bone marrow, adipose tissue, umbilical cord, and dental pulp) with the capacity to self-renew. Historically, researchers have isolated these stem cells using various methods and a s...

Vascular Pericytes

Figure 1. Vascular pericyte overview. Pericytes surround blood vessel capillaries and endothelial cells, neurons and astrocytes within the blood-brain-barrier (BBB) neurovascular unit. Pericytes, also known as mural cells, are multipotent perivascular cells that play an important role in the develop...

Cell Biology | Highest Quality and Largest Selection of Biomolecules

Enhance your understanding of biology with our world-class biomolecules. Our portfolio includes over 50,000 primary antibodies, 12,000 of which are Prestige Antibodies® powered by Atlas Antibodies, as well as a vast range of proteins, bioactive peptides, and kinases. Offering over 3,000 Bioactive Sm...

Live Cell Calcium Indicators

Calcium ions (Ca2+) play vital cellular physiology roles in signal transduction pathways, in neurotransmitter release, in contraction of all muscle cell types, as enzyme cofactors, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable...

Benchtop flow cytometry facilitates discrimination between induction of DNA damage, oxidative stress and protein misfolding using a novel multicell GFP reporter assay

Giel Hendriks1, Remco S. Derr1, Harry Vrieling2, Robin T. Clark3 1Toxys B.V., Robert Boyleweg 4, 2333 CG, Leiden, The Netherlands 2Department of Human Genetics, Leiden University Medical Center, PO Box 9600, 2300 RC, Leiden, The Netherlands 3MilliporeSigma, a business of Merck KGaA, Darmstadt, Germa...

Vector Maps | FLAG® and 3xFLAG® Overview | System for Detection and Purification of Proteins

A Proven System for Detection and Purification of Proteins The FLAG Expression System is an established way to express, purify and detect recombinant fusion proteins. FLAG and 3xFLAG have proven utility in numerous applications such as Western blotting, immunocytochemistry, immunoprecipitation, flow...

PBMC Macrophage Differentiation

Macrophages are tissue-resident professional phagocytes and antigen-presenting cells (APC), which differentiate from circulating peripheral blood monocytes. They perform important active and regulatory functions in innate as well as adaptive immunity.1 Activated macrophages of different phenotypes a...

Target Discovery for Early Drug Identification

Is your lab having difficulty discovering novel targets? Our broad portfolio of assays, reagents, and libraries can help you find the right lock so you can begin the work to unlock it. From the Sanger Whole Genome CRISPR Library to Duolink® PLA to measure protein-protein interactions, and bioactive ...

Efficient Delivery of Sigma-Aldrich CRISPR via a Non-Liposomal Polymeric Transfection Reagent, TransIT-CRISPR®

Efficient intracellular delivery of CRISPR/Cas9 components is needed to achieve high nuclease activity for genome editing. The potent delivery of CRISPR Cas9 plasmids, RNA and RNP (ribonucleoprotein) Complexes into two adherent cell lines, U2OS and HepaRG was achieved using TransIT-CRISPR. Sigma-Ald...

Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

Download BioFiles v6 n5 (4.66 Mb PDF) The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate a...

Target Identification & Validation for Early Drug Discovery

Early stages of drug discovery start with initial steps of target identification and moves to the later stages of lead optimization. Multiple sources including academic research, clinical works and commercial sector help in the identification of a suitable disease target. The chosen target is then u...

How to Optimize Duolink® PLA for Flow Cytometry Detection

Adaptation of Duolink® in situ proximity ligation assay (PLA) into flow cytometry workflows allows quantitative detection of protein-protein interactions, protein post-translational modifications, and protein expression with greater statistical power. However, to successfully execute Duolink® PLA fo...

Characterisation of Antibodies with BRAND® PCR Plates

Introduction The generation of fully human antibodies with unique functional properties that are exploitable for tailored therapeutic interventions remains a major challenge in the antibody technology field. However, it is possible to isolate these antibodies from human B-cellls in contact with spec...

Duolink® Webinar

Duolink® proximity ligation assay (PLA) technology allows you to visualize protein interactions with cellular localization and quantities by amplifying signals corresponding to single and post-translational protein events. With 1000x sensitivity and high specificity, this protein detection technolog...

Bioactive Small Molecules Literature References

Cell Biology Applications Center Bioactive Small Molecules Literature References Looking for current application references for Bioactive Small Molecules? Look no further! • Apoptosis • Kinase Phosphatase Biology • Neuroscience Apoptosis Publications: Click on the title to view the abstract on PubMe...

FSL Construct Protocol

The following protocol describes the generic procedure for the insertion of FSL constructs (Figure 1) into biological membranes. For simplicity the protocol will only refer to cells (kodecytes), which are at a 100% concentration. However, the term cells is interchangeable with virions (kodevirions) ...

Lemongrass (Cymbopogon) | Plant Profiler

Synonyms / Common Names / Related Terms Abafado (Portuguese), alpha-citral, alpha-terpineole, Andropogon citratus, Andropogon nardus, bai mak nao (Lao), beta-citral (neral), beta-myrcene, bhustrina (Indian), British Indian lemongrass, capim-cidrao, carene-2, caspase-3, Ceylon citronella grass, citra...

Stem Cell Culture Glossary and Terms

Related Products • EBiSC Human iPS Cell Lines • Custom Engineered iPSC Lines • Pluripotent Stem Cell Culture • Neural Stem Cell Culture • Mesenchymal Stem Cell Culture • Hematopoietic Stem Cell Culture • Stem Cell Protocols • MEF Feeder Cells • 3D Cell Culture • Cell Culture Media • Stericup® Media ...

Choosing Lipophilic Dye Kit for Cell Tracking

• • Download PDF • Supplemental Information Katharine A. Muirhead, Joseph D. Tario, Jr., Paul K. Wallace Table S1. Non-Perturbing Membrane-Dye Labeling Conditions for Selected Cell Types1 Cell Type Final Cell Concentration Final Dye Concentration hPBMC* (large cell number) 1 x 107 cells/mL 3 x 107 c...